JP2015506699A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015506699A5 JP2015506699A5 JP2014555774A JP2014555774A JP2015506699A5 JP 2015506699 A5 JP2015506699 A5 JP 2015506699A5 JP 2014555774 A JP2014555774 A JP 2014555774A JP 2014555774 A JP2014555774 A JP 2014555774A JP 2015506699 A5 JP2015506699 A5 JP 2015506699A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- nucleic acid
- polypeptide
- item
- acid molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261594149P | 2012-02-02 | 2012-02-02 | |
| US61/594,149 | 2012-02-02 | ||
| PCT/US2013/024406 WO2013116698A2 (en) | 2012-02-02 | 2013-02-01 | High throughput screen for biologically active polypeptides |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015506699A JP2015506699A (ja) | 2015-03-05 |
| JP2015506699A5 true JP2015506699A5 (https=) | 2016-03-10 |
| JP6196241B2 JP6196241B2 (ja) | 2017-09-13 |
Family
ID=47750048
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014555774A Expired - Fee Related JP6196241B2 (ja) | 2012-02-02 | 2013-02-01 | 生物学的に活性なポリペプチドのハイスループットスクリーニング |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9701959B2 (https=) |
| EP (1) | EP2809783A2 (https=) |
| JP (1) | JP6196241B2 (https=) |
| CA (1) | CA2861387A1 (https=) |
| WO (1) | WO2013116698A2 (https=) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| JP2009536313A (ja) | 2006-01-11 | 2009-10-08 | レインダンス テクノロジーズ, インコーポレイテッド | ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法 |
| US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| ATE540750T1 (de) | 2006-05-11 | 2012-01-15 | Raindance Technologies Inc | Mikrofluidische vorrichtung und verfahren |
| US8772046B2 (en) | 2007-02-06 | 2014-07-08 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| US8592221B2 (en) | 2007-04-19 | 2013-11-26 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
| EP2315629B1 (en) | 2008-07-18 | 2021-12-15 | Bio-Rad Laboratories, Inc. | Droplet libraries |
| US12038438B2 (en) | 2008-07-18 | 2024-07-16 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| EP3415235B1 (en) | 2009-03-23 | 2025-11-12 | Bio-Rad Laboratories, Inc. | Manipulation of microfluidic droplets |
| CA2789425C (en) | 2010-02-12 | 2020-04-28 | Raindance Technologies, Inc. | Digital analyte analysis with polymerase error correction |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| WO2012045012A2 (en) | 2010-09-30 | 2012-04-05 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| EP3859011A1 (en) | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Methods for forming mixed droplets |
| EP3736281A1 (en) | 2011-02-18 | 2020-11-11 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| EP3709018A1 (en) | 2011-06-02 | 2020-09-16 | Bio-Rad Laboratories, Inc. | Microfluidic apparatus for identifying components of a chemical reaction |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| WO2013134261A1 (en) | 2012-03-05 | 2013-09-12 | President And Fellows Of Harvard College | Systems and methods for epigenetic sequencing |
| US9290552B2 (en) | 2012-12-21 | 2016-03-22 | Dna Twopointo, Inc. | Fluorescent and colored proteins and methods for using them |
| US8975042B2 (en) * | 2012-12-21 | 2015-03-10 | Dna Twopointo, Inc. | Fluorescent and colored proteins and methods for using them |
| US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
| US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
| WO2015164212A1 (en) * | 2014-04-21 | 2015-10-29 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| EP3268462B1 (en) * | 2015-03-11 | 2021-08-11 | The Broad Institute, Inc. | Genotype and phenotype coupling |
| WO2016168584A1 (en) | 2015-04-17 | 2016-10-20 | President And Fellows Of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| WO2016187466A1 (en) * | 2015-05-20 | 2016-11-24 | Invenra, Inc. | Methods, apparatus and compositions for expression and high throughput screening |
| US11123740B2 (en) | 2015-06-29 | 2021-09-21 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis |
| WO2017117358A1 (en) * | 2015-12-30 | 2017-07-06 | Bio-Rad Laboratories, Inc. | Digital protein quantification |
| ES2996699T3 (en) * | 2016-04-12 | 2025-02-13 | Medimmune Llc | Immune repertoire mining |
| KR102657960B1 (ko) * | 2016-10-18 | 2024-04-16 | 삼성전자주식회사 | 힘 전달 프레임 및 이를 포함하는 운동 보조 장치 |
| WO2018098438A1 (en) | 2016-11-28 | 2018-05-31 | Arizona Board Of Regents On Behalf Of Arizona State University | Systems and methods related to continuous flow droplet reaction |
| JP7360324B2 (ja) | 2016-12-23 | 2023-10-12 | ビステラ, インコーポレイテッド | 結合ポリペプチドおよびそれを作製する方法 |
| KR20200004335A (ko) * | 2017-05-05 | 2020-01-13 | 스키피오 바이오사이언스 | 하이드로겔 내의 별개의 생물학적 단위를 트래핑하고 바코딩하는 방법 |
| CN111386351A (zh) | 2017-09-22 | 2020-07-07 | 华盛顿大学 | 细胞分子的原位组合标记 |
| CN112088212A (zh) * | 2018-02-15 | 2020-12-15 | 埃内沃夫公司 | 传感器系统 |
| SG11202009478PA (en) | 2018-04-02 | 2020-10-29 | Dropworks Inc | Systems and methods for serial flow emulsion processes |
| WO2020193368A1 (en) * | 2019-03-22 | 2020-10-01 | Qiagen Gmbh | Method for improving the amplification efficiency of bead-based emulsion pcr (empcr) |
| EP3975978A4 (en) * | 2019-06-03 | 2023-06-28 | Dropworks, Inc. | Systems and methods for stabilizing emulsions |
| AU2020361681A1 (en) | 2019-10-10 | 2022-05-05 | 1859, Inc. | Methods and systems for microfluidic screening |
| US20230287490A1 (en) * | 2020-07-28 | 2023-09-14 | Protillion Biosciences, Inc. | Systems and methods for assaying a plurality of polypeptides |
| CA3190249A1 (en) * | 2020-08-21 | 2022-02-24 | Liang SCHWEIZER | Functional screening using droplet-based microfluidics |
Family Cites Families (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3826364A (en) | 1972-05-22 | 1974-07-30 | Univ Leland Stanford Junior | Particle sorting method and apparatus |
| US4284412A (en) | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
| US4498766A (en) | 1982-03-25 | 1985-02-12 | Becton, Dickinson And Company | Light beam focal spot elongation in flow cytometry devices |
| US4661913A (en) | 1984-09-11 | 1987-04-28 | Becton, Dickinson And Company | Apparatus and method for the detection and classification of articles using flow cytometry techniques |
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4857451A (en) | 1984-12-24 | 1989-08-15 | Flow Cytometry Standards Corporation | Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor |
| US4774189A (en) | 1984-12-24 | 1988-09-27 | Flow Cytometry Standards Corp. | Fluorescent calibration microbeads simulating stained cells |
| US4767206A (en) | 1984-12-24 | 1988-08-30 | Flow Cytometry Standards Corporation | Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4989977A (en) | 1985-07-29 | 1991-02-05 | Becton, Dickinson And Company | Flow cytometry apparatus with improved light beam adjustment |
| US4714682A (en) | 1985-12-11 | 1987-12-22 | Flow Cytometry Standards Corporation | Fluorescent calibration microbeads simulating stained cells |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| AU622104B2 (en) | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| AU622426B2 (en) | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
| WO1989006700A1 (en) | 1988-01-21 | 1989-07-27 | Genentech, Inc. | Amplification and detection of nucleic acid sequences |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5858652A (en) | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
| US4932207A (en) | 1988-12-28 | 1990-06-12 | Sundstrand Corporation | Segmented seal plate for a turbine engine |
| US5160974A (en) | 1990-06-25 | 1992-11-03 | Flow Science, Inc. | Closed sample cell for use in flow cytometry |
| US5478722A (en) | 1991-02-17 | 1995-12-26 | The Curators Of The University Of Missouri | Preserved cell preparations for flow cytometry and immunology |
| US5846709A (en) | 1993-06-15 | 1998-12-08 | Imclone Systems Incorporated | Chemical process for amplifying and detecting nucleic acid sequences |
| FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
| US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
| NZ287118A (en) | 1994-05-28 | 1999-01-28 | Tepnel Medical Ltd | Method of detecting and amplifying oligonucleotides |
| US5942391A (en) | 1994-06-22 | 1999-08-24 | Mount Sinai School Of Medicine | Nucleic acid amplification method: ramification-extension amplification method (RAM) |
| JP3102800B2 (ja) | 1994-08-19 | 2000-10-23 | パーキン−エルマー コーポレイション | 増幅及び連結反応の共役法 |
| US5935825A (en) | 1994-11-18 | 1999-08-10 | Shimadzu Corporation | Process and reagent for amplifying nucleic acid sequences |
| US5843650A (en) | 1995-05-01 | 1998-12-01 | Segev; David | Nucleic acid detection and amplification by chemical linkage of oligonucleotides |
| WO1998004689A1 (en) | 1995-07-31 | 1998-02-05 | Urocor, Inc. | Biomarkers and targets for diagnosis, prognosis and management of prostate disease |
| US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| US20030215798A1 (en) | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
| US5612473A (en) | 1996-01-16 | 1997-03-18 | Gull Laboratories | Methods, kits and solutions for preparing sample material for nucleic acid amplification |
| US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| US5939291A (en) | 1996-06-14 | 1999-08-17 | Sarnoff Corporation | Microfluidic method for nucleic acid amplification |
| US5849546A (en) | 1996-09-13 | 1998-12-15 | Epicentre Technologies Corporation | Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates |
| US5849497A (en) | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| US5866366A (en) | 1997-07-01 | 1999-02-02 | Smithkline Beecham Corporation | gidB |
| JP4298912B2 (ja) | 1997-07-07 | 2009-07-22 | メディカル リサーチ カウンシル | invitro選別法 |
| US5916776A (en) | 1997-08-27 | 1999-06-29 | Sarnoff Corporation | Amplification method for a polynucleotide |
| US5932451A (en) | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| US6210910B1 (en) | 1998-03-02 | 2001-04-03 | Trustees Of Tufts College | Optical fiber biosensor array comprising cell populations confined to microcavities |
| GB9900298D0 (en) | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
| GB9917027D0 (en) * | 1999-07-20 | 1999-09-22 | Affibody Technology Sweeden Ab | In vitro selection and optional identification of polypeptides using solid support carriers |
| AU2001278613B2 (en) | 2000-08-15 | 2006-09-07 | Discerna Limited | Functional protein arrays |
| GB0022458D0 (en) | 2000-09-13 | 2000-11-01 | Medical Res Council | Directed evolution method |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| GB0221053D0 (en) | 2002-09-11 | 2002-10-23 | Medical Res Council | Single-molecule in vitro evolution |
| WO2005003375A2 (en) | 2003-01-29 | 2005-01-13 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| WO2004067742A1 (ja) * | 2003-01-31 | 2004-08-12 | Keio University | 切断可能な対応付け分子およびそれを用いるスクリーニング方法 |
| GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
| GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
| US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
| WO2005007796A2 (en) | 2003-07-21 | 2005-01-27 | Molecular Cytomics Ltd. | Improved multiwell plate |
| US7585815B2 (en) | 2003-07-24 | 2009-09-08 | Lawrence Livermore National Security, Llc | High throughput protein production screening |
| JP4430076B2 (ja) | 2003-10-01 | 2010-03-10 | イーティーエイチ・チューリッヒ | ポリペプチドの試験管内進化方法 |
| GB0325653D0 (en) | 2003-11-03 | 2003-12-10 | Medical Res Council | CST emulsions |
| CN101014716B (zh) | 2003-11-20 | 2015-06-10 | 小利兰·斯坦福大学托管委员会 | 体外蛋白质合成的改进方法 |
| WO2005049787A2 (en) | 2003-11-24 | 2005-06-02 | Yeda Research And Development Co.Ltd. | Compositions and methods for in vitro sorting of molecular and cellular libraries |
| US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| GB0511717D0 (en) | 2005-06-09 | 2005-07-13 | Babraham Inst | Repeatable protein arrays |
| WO2009145818A1 (en) * | 2008-03-31 | 2009-12-03 | Pacific Biosciences Of California, Inc | Single molecule loading methods and compositions |
| WO2009123756A1 (en) | 2008-04-05 | 2009-10-08 | Single Cell Technology, Inc. | Multi-well system |
| EP2315629B1 (en) | 2008-07-18 | 2021-12-15 | Bio-Rad Laboratories, Inc. | Droplet libraries |
| US8312249B1 (en) | 2008-10-10 | 2012-11-13 | Apple Inc. | Dynamic trampoline and structured code generation in a signed code environment |
| EP2452195A4 (en) | 2009-07-07 | 2012-12-05 | Agency Science Tech & Res | METHODS OF IDENTIFYING A PAIR OF LIAISON PARTNERS |
| FR2957821B1 (fr) | 2010-03-24 | 2014-08-29 | Inst Francais Du Petrole | Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives |
-
2013
- 2013-02-01 US US14/375,943 patent/US9701959B2/en not_active Expired - Fee Related
- 2013-02-01 EP EP13706121.4A patent/EP2809783A2/en not_active Withdrawn
- 2013-02-01 WO PCT/US2013/024406 patent/WO2013116698A2/en not_active Ceased
- 2013-02-01 CA CA2861387A patent/CA2861387A1/en not_active Abandoned
- 2013-02-01 JP JP2014555774A patent/JP6196241B2/ja not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015506699A5 (https=) | ||
| Wu et al. | Profiling surface proteins on individual exosomes using a proximity barcoding assay | |
| Lee et al. | Plasma/serum proteomics: depletion strategies for reducing high-abundance proteins for biomarker discovery | |
| Wang et al. | Multiparameter particle display (MPPD): a quantitative screening method for the discovery of highly specific aptamers | |
| JP7128792B2 (ja) | ハイスループットなレセプター:リガンド同定の方法 | |
| Gehring et al. | High-throughput biosensors for multiplexed food-borne pathogen detection | |
| Bole et al. | Advanced materials for the recognition and capture of whole cells and microorganisms | |
| Liébana et al. | Electrochemical immunosensors, genosensors and phagosensors for Salmonella detection | |
| Lucas et al. | Lab-on-a-chip immunoassay for multiple antibodies using microsphere light scattering and quantum dot emission | |
| Peikon et al. | Using high-throughput barcode sequencing to efficiently map connectomes | |
| González-González et al. | Aqueous two-phase systems strategies to establish novel bioprocesses for stem cells recovery | |
| Laghmouchi et al. | Long‐term in vitro persistence of magnetic properties after magnetic bead‐based cell separation of T cells | |
| JP2017514481A (ja) | 標的細胞を単離する方法 | |
| Lee et al. | Development of the “Three-step MACS”: a novel strategy for isolating rare cell populations in the absence of known cell surface markers from complex animal tissue | |
| DK2856162T3 (en) | Microplates with enhanced immobilization properties, controlled through magnetic field | |
| Choi | Advances in single cell technologies in immunology | |
| Rödiger et al. | Fluorescence dye adsorption assay to quantify carboxyl groups on the surface of poly (methyl methacrylate) microbeads | |
| Andrews et al. | Morphological landscapes from high content imaging reveal cytokine priming strategies that enhance mesenchymal stromal cell immunosuppression | |
| Chen et al. | Graphene Oxide Nanosheets Modified with Single‐Domain Antibodies for Rapid and Efficient Capture of Cells | |
| CN102692505A (zh) | 一种改进的免疫共沉淀技术方法 | |
| Hwang et al. | Aptamer-conjugated live human immune cell based biosensors for the accurate detection of C-reactive protein | |
| Glick et al. | Pathogen receptor discovery with a microfluidic human membrane protein array | |
| Gui et al. | A Functional Assay for Mining Noninhibitory Enzyme Ligands from One Bead One Compound Libraries: Application to E3 Ubiquitin Ligases | |
| Zhao et al. | Rapid Single‐Cell Proteomics Using Nanoconfined Enzyme Reactors on a Microscale Digital Microfluidics Platform | |
| Goodwin et al. | 1D genome sequencing on the oxford nanopore MinION |