CA2861387A1 - High throughput screen for biologically active polypeptides - Google Patents

High throughput screen for biologically active polypeptides Download PDF

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Publication number
CA2861387A1
CA2861387A1 CA2861387A CA2861387A CA2861387A1 CA 2861387 A1 CA2861387 A1 CA 2861387A1 CA 2861387 A CA2861387 A CA 2861387A CA 2861387 A CA2861387 A CA 2861387A CA 2861387 A1 CA2861387 A1 CA 2861387A1
Authority
CA
Canada
Prior art keywords
nucleic acid
cells
polypeptide
acid molecules
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2861387A
Other languages
English (en)
French (fr)
Inventor
Roland Green
Bryan Glaser
Ivar Meyvantsson
Kimberly KAUFMAN
Madison GREEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Invenra Inc
Original Assignee
Invenra Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invenra Inc filed Critical Invenra Inc
Publication of CA2861387A1 publication Critical patent/CA2861387A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2861387A 2012-02-02 2013-02-01 High throughput screen for biologically active polypeptides Abandoned CA2861387A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261594149P 2012-02-02 2012-02-02
US61/594,149 2012-02-02
PCT/US2013/024406 WO2013116698A2 (en) 2012-02-02 2013-02-01 High throughput screen for biologically active polypeptides

Publications (1)

Publication Number Publication Date
CA2861387A1 true CA2861387A1 (en) 2013-08-08

Family

ID=47750048

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2861387A Abandoned CA2861387A1 (en) 2012-02-02 2013-02-01 High throughput screen for biologically active polypeptides

Country Status (5)

Country Link
US (1) US9701959B2 (https=)
EP (1) EP2809783A2 (https=)
JP (1) JP6196241B2 (https=)
CA (1) CA2861387A1 (https=)
WO (1) WO2013116698A2 (https=)

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Also Published As

Publication number Publication date
US20150018236A1 (en) 2015-01-15
JP6196241B2 (ja) 2017-09-13
WO2013116698A2 (en) 2013-08-08
WO2013116698A3 (en) 2013-10-17
JP2015506699A (ja) 2015-03-05
US9701959B2 (en) 2017-07-11
EP2809783A2 (en) 2014-12-10

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Effective date: 20190201