JP2014530626A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2014530626A5 JP2014530626A5 JP2014537306A JP2014537306A JP2014530626A5 JP 2014530626 A5 JP2014530626 A5 JP 2014530626A5 JP 2014537306 A JP2014537306 A JP 2014537306A JP 2014537306 A JP2014537306 A JP 2014537306A JP 2014530626 A5 JP2014530626 A5 JP 2014530626A5
- Authority
- JP
- Japan
- Prior art keywords
- vol
- cells
- gene product
- expression
- isolated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims 24
- 108090000623 proteins and genes Proteins 0.000 claims 12
- 230000014509 gene expression Effects 0.000 claims 9
- DCXXMTOCNZCJGO-UHFFFAOYSA-N tristearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC DCXXMTOCNZCJGO-UHFFFAOYSA-N 0.000 claims 8
- 150000007523 nucleic acids Chemical class 0.000 claims 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 6
- 229910052799 carbon Inorganic materials 0.000 claims 6
- 235000014113 dietary fatty acids Nutrition 0.000 claims 6
- 229930195729 fatty acid Natural products 0.000 claims 6
- 239000000194 fatty acid Substances 0.000 claims 6
- 150000004665 fatty acids Chemical class 0.000 claims 5
- 238000006243 chemical reaction Methods 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 238000012239 gene modification Methods 0.000 claims 3
- 230000005017 genetic modification Effects 0.000 claims 3
- 235000013617 genetically modified food Nutrition 0.000 claims 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 2
- 101150023395 DGA1 gene Proteins 0.000 claims 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 2
- 238000009825 accumulation Methods 0.000 claims 2
- 230000009286 beneficial effect Effects 0.000 claims 2
- 230000033228 biological regulation Effects 0.000 claims 2
- 239000008103 glucose Substances 0.000 claims 2
- 230000001105 regulatory effect Effects 0.000 claims 2
- 210000005253 yeast cell Anatomy 0.000 claims 2
- 101150058703 ACC1 gene Proteins 0.000 claims 1
- 241000195493 Cryptophyta Species 0.000 claims 1
- 101150048395 SCD gene Proteins 0.000 claims 1
- 108700009124 Transcription Initiation Site Proteins 0.000 claims 1
- 241000235015 Yarrowia lipolytica Species 0.000 claims 1
- 210000000577 adipose tissue Anatomy 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000006378 damage Effects 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 125000005313 fatty acid group Chemical group 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 230000002018 overexpression Effects 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 150000003626 triacylglycerols Chemical class 0.000 claims 1
- 210000003934 vacuole Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161548901P | 2011-10-19 | 2011-10-19 | |
| US61/548,901 | 2011-10-19 | ||
| US201261663263P | 2012-06-22 | 2012-06-22 | |
| US61/663,263 | 2012-06-22 | ||
| PCT/US2012/061101 WO2013059649A1 (en) | 2011-10-19 | 2012-10-19 | Engineered microbes and methods for microbial oil production |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014530626A JP2014530626A (ja) | 2014-11-20 |
| JP2014530626A5 true JP2014530626A5 (https=) | 2015-12-10 |
| JP6294827B2 JP6294827B2 (ja) | 2018-03-14 |
Family
ID=48141406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014537306A Expired - Fee Related JP6294827B2 (ja) | 2011-10-19 | 2012-10-19 | 操作された微生物および微生物油生成のための方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US8951776B2 (https=) |
| EP (2) | EP2768954B1 (https=) |
| JP (1) | JP6294827B2 (https=) |
| KR (1) | KR102036389B1 (https=) |
| CN (2) | CN109468241A (https=) |
| BR (1) | BR112014009245A2 (https=) |
| RU (1) | RU2652875C2 (https=) |
| WO (1) | WO2013059649A1 (https=) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8765404B2 (en) * | 2010-03-02 | 2014-07-01 | Massachusetts Institute Of Technology | Microbial engineering for the production of fatty acids and fatty acid derivatives |
| JP6294827B2 (ja) * | 2011-10-19 | 2018-03-14 | マサチューセッツ インスティテュート オブ テクノロジー | 操作された微生物および微生物油生成のための方法 |
| JP2015512653A (ja) * | 2012-04-09 | 2015-04-30 | ビーピー・バイオフューエルズ・ユーケイ・リミテッド | バイオ燃料及び他の再生可能な材料の産生のための低多糖微生物 |
| CA2911158A1 (en) * | 2013-05-03 | 2014-11-06 | Board Of Regents, The University Of Texas System | Compositions and methods for fungal lipid production |
| AU2015252916B2 (en) | 2014-05-01 | 2018-08-09 | Ginkgo Bioworks, Inc. | Increasing cellular lipid production by increasing the activity of diacylglycerol acyltransferase and decreasing the activity of triacylglycerol lipase |
| EP3149153B1 (en) | 2014-05-29 | 2022-07-13 | Ginkgo Bioworks, Inc. | Increasing lipid production in oleaginous yeast |
| KR102311681B1 (ko) | 2015-07-28 | 2021-10-12 | 삼성전자주식회사 | 내산성을 갖는 효모 세포, 그를 이용하여 유기산을 생산하는 방법 및 상기 내산성 효모 세포를 생산하는 방법 |
| JP2017039078A (ja) * | 2015-08-19 | 2017-02-23 | 太平洋セメント株式会社 | 排水の処理方法 |
| US11142770B2 (en) | 2015-10-20 | 2021-10-12 | Massachusetts Institute Of Technology | Isolated oleaginous yeast |
| CA3069697A1 (en) | 2017-07-13 | 2019-01-17 | Radici Chimica S.P.A. | Biological methods for modifying cellular carbon flux |
| CN107904199A (zh) * | 2017-11-30 | 2018-04-13 | 杭州唯铂莱生物科技有限公司 | 用于合成山扁豆酸的工程改造的宿主细胞和方法 |
| CN110358692B (zh) * | 2018-04-09 | 2021-07-27 | 中国科学院青岛生物能源与过程研究所 | 生产神经酸的重组酵母菌株及其应用 |
| WO2019241322A1 (en) * | 2018-06-13 | 2019-12-19 | Manus Bio, Inc. | Enzymes, methods, and host cells for producing carminic acid |
| CN109929870B (zh) * | 2019-02-20 | 2021-03-16 | 天津大学 | 糖代谢与脂质代谢协同提高解脂耶氏酵母合成脂肪酸衍生物的产量的应用 |
| GB202010630D0 (en) * | 2020-07-10 | 2020-08-26 | Imp College Innovations Ltd | Methods |
| US20240150800A1 (en) * | 2022-11-04 | 2024-05-09 | Viridos, Inc. | Recombinant algae having high biomass and lipid productivity |
| WO2025229117A1 (en) | 2024-05-02 | 2025-11-06 | Vib Vzw | Yeast for increased lipid production |
| WO2026057627A1 (en) * | 2024-09-11 | 2026-03-19 | Vib Vzw | Yeast for increased lipid production |
| CN119799810A (zh) * | 2025-03-14 | 2025-04-11 | 山西鑫晋商生物科技有限公司 | 基于发酵工艺优化的二酯油微生物发酵方法及系统 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE356213T1 (de) * | 1999-04-01 | 2007-03-15 | Basf Plant Science Gmbh | Enzyme des triacylglycerol-biosynthesewegs und dafür kodierende rekombinante dna moleküle |
| US7267976B2 (en) * | 2003-07-02 | 2007-09-11 | E.I. Du Pont De Nemours And Company | Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts |
| WO2005049805A2 (en) * | 2003-11-14 | 2005-06-02 | E.I. Dupont De Nemours And Company | Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast |
| CA2568689A1 (en) * | 2004-06-04 | 2005-12-15 | Fluxome Sciences A/S | Metabolically engineered cells for the production of polyunsaturated fatty acids |
| US7198937B2 (en) * | 2004-11-04 | 2007-04-03 | E. I. Du Pont De Nemours And Company | Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms |
| US7273746B2 (en) * | 2004-11-04 | 2007-09-25 | E.I. Dupont De Nemours And Company | Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms |
| US7550286B2 (en) | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
| EP2166100B1 (en) * | 2005-03-08 | 2012-07-18 | BASF Plant Science GmbH | Expression enhancing intron sequences |
| EP2147109B1 (en) * | 2007-05-24 | 2014-03-19 | E. I. Du Pont de Nemours and Company | Dgat genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean |
| WO2009073822A2 (en) * | 2007-12-04 | 2009-06-11 | The Ohio State University Research Foundation | Molecular approaches for the optimization of biofuel production |
| WO2010025374A2 (en) * | 2008-08-29 | 2010-03-04 | E. I. Du Pont De Nemours And Company | Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms |
| US8394621B2 (en) | 2008-10-23 | 2013-03-12 | Matrix Genetrics, LLC | Modified photosynthetic microorganisms for producing triglycerides |
| AU2009330014A1 (en) * | 2008-12-23 | 2011-08-11 | Matrix Genetics, Llc | Modified photosynthetic microorganisms with reduced glycogen and their use in producing carbon-based products |
| EP2470665A4 (en) | 2009-08-28 | 2013-04-17 | Phycal Inc | BIOFUEL FROM RECOMBINANT OIL-CONTAINING ALGAE USING SUGAR CARBON SOURCES |
| EP2516638A1 (en) * | 2009-12-24 | 2012-10-31 | E.I. Du Pont De Nemours And Company | Plant membrane bound o-acyl transferase (mboat) family protein sequences and their uses for altering fatty acid compositions |
| WO2011088364A2 (en) | 2010-01-15 | 2011-07-21 | Massachuseits Institute Of Technology | Bioprocess and microbe engineering for total carbon utilization in biofuelproduction |
| US8765404B2 (en) | 2010-03-02 | 2014-07-01 | Massachusetts Institute Of Technology | Microbial engineering for the production of fatty acids and fatty acid derivatives |
| TW201144442A (en) | 2010-05-17 | 2011-12-16 | Dow Agrosciences Llc | Production of DHA and other LC-PUFAs in plants |
| US9523096B2 (en) | 2010-12-20 | 2016-12-20 | Matrix Genetics, Llc | Modified photosynthetic microorganisms for producing lipids |
| WO2012087963A1 (en) | 2010-12-20 | 2012-06-28 | Targeted Growth, Inc. | Modified photosynthetic microorganisms for producing lipids |
| TW201307553A (zh) | 2011-07-26 | 2013-02-16 | Dow Agrosciences Llc | 在植物中生產二十二碳六烯酸（ｄｈａ）及其他長鏈多元不飽和脂肪酸（ｌｃ－ｐｕｆａ）之技術 |
| JP6294827B2 (ja) * | 2011-10-19 | 2018-03-14 | マサチューセッツ インスティテュート オブ テクノロジー | 操作された微生物および微生物油生成のための方法 |
| EP2809790A4 (en) * | 2012-02-03 | 2015-11-18 | Matrix Genetics Llc | MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR THE CONTINUOUS PRODUCTION OF CARBON-CONTAINING COMPOUNDS |
| WO2013134139A1 (en) * | 2012-03-09 | 2013-09-12 | Matrix Genetics, Llc | Modified diacylglycerol acyltransferase proteins and methods of use thereof |
| WO2013192520A1 (en) * | 2012-06-22 | 2013-12-27 | Massachusetts Institute Of Technology | Engineered microbes and methods for microbial oil overproduction from cellulosic materials |
-
2012
- 2012-10-19 JP JP2014537306A patent/JP6294827B2/ja not_active Expired - Fee Related
- 2012-10-19 CN CN201811471219.4A patent/CN109468241A/zh active Pending
- 2012-10-19 EP EP12840947.1A patent/EP2768954B1/en not_active Not-in-force
- 2012-10-19 EP EP18161442.1A patent/EP3388516A1/en not_active Withdrawn
- 2012-10-19 US US13/656,086 patent/US8951776B2/en active Active
- 2012-10-19 WO PCT/US2012/061101 patent/WO2013059649A1/en not_active Ceased
- 2012-10-19 BR BR112014009245-1A patent/BR112014009245A2/pt not_active Application Discontinuation
- 2012-10-19 CN CN201280060959.3A patent/CN104160020A/zh active Pending
- 2012-10-19 RU RU2014119784A patent/RU2652875C2/ru active
- 2012-10-19 KR KR1020147013207A patent/KR102036389B1/ko not_active Expired - Fee Related
-
2014
- 2014-11-19 US US14/547,304 patent/US9862977B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2014530626A5 (https=) | ||
| Yan et al. | Overexpression of acetyl-CoA synthetase increased the biomass and fatty acid proportion in microalga Schizochytrium | |
| Zheng et al. | Lipid and carotenoid production by the Rhodosporidium toruloides mutant in cane molasses | |
| Sakuradani et al. | Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S-4 | |
| Wang et al. | Optimizing eicosapentaenoic acid production by grafting a heterologous polyketide synthase pathway in the thraustochytrid Aurantiochytrium | |
| Cui et al. | Tung tree (Vernicia fordii, Hemsl.) genome and transcriptome sequencing reveals co-ordinate up-regulation of fatty acid β-oxidation and triacylglycerol biosynthesis pathways during eleostearic acid accumulation in seeds | |
| RU2014119784A (ru) | Сконструированные микробы и способы получения микробного масла | |
| JP7261815B2 (ja) | ネルボン酸を生産する組換え酵母菌株およびその使用 | |
| US20130102040A1 (en) | Use of endogenous promoters in genetic engineering of nannochloropsis gaditana | |
| CN108271383A (zh) | 真菌细胞和生产非常长链脂肪酸衍生产物的方法 | |
| JP2010142240A5 (https=) | ||
| JP6448682B2 (ja) | アルデヒドおよび対応するアルコールの微生物合成 | |
| US9926540B2 (en) | Myrmecia incisa reisigl diacylglycerol acyltransferase gene sequence and use thereof | |
| US10081821B2 (en) | Methods for efficient production of polyunsaturated fatty acids (PUFA) in Rhodosporidium and Rhodotorula species | |
| CN105026546A (zh) | 重组生物 | |
| Sha et al. | Efficient secretion of lipase r27RCL in Pichia pastoris by enhancing the disulfide bond formation pathway in the endoplasmic reticulum | |
| Zhou et al. | Dynamic regulation combined with systematic metabolic engineering for high-level palmitoleic acid accumulation in oleaginous yeast | |
| Legendre et al. | Evolution of a desaturase involved in female pheromonal cuticular hydrocarbon biosynthesis and courtship behavior in Drosophila | |
| Periyasamy et al. | Isolation, characterization and evaluation of the Pichia pastoris sorbitol dehydrogenase promoter for expression of heterologous proteins | |
| JP2014527803A (ja) | トリアシルグリセロール（ｔａｇ）リパーゼの遺伝子操作による微細藻類における脂質含量の増大 | |
| Zhang et al. | Manipulation of triacylglycerol biosynthesis in Nannochloropsis oceanica by overexpressing an Arabidopsis thaliana diacylglycerol acyltransferase gene | |
| Xie et al. | Sustainable production of omega-3 eicosapentaenoic acid by fermentation of metabolically engineered Yarrowia lipolytica | |
| Cheng et al. | Establishment of a transgene expression system for the marine microalga Schizochytrium by 18S rDNA-targeted homologous recombination | |
| CN101353661B (zh) | 具有△6脂肪酸脱氢酶功能的基因及其应用 | |
| CN117467704B (zh) | 构建apod条件敲入小鼠模型的方法 |