JP2014519811A - チオラーゼ、ＨＭＧ−ＣｏＡ合成酵素及びＨＭＧ−ＣｏＡ還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産 - Google Patents
チオラーゼ、ＨＭＧ−ＣｏＡ合成酵素及びＨＭＧ−ＣｏＡ還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産 Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01034—Hydroxymethylglutaryl-CoA reductase (NADPH) (1.1.1.34)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01016—Acetyl-CoA C-acyltransferase (2.3.1.16)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
- C12Y203/0301—Hydroxymethylglutaryl-CoA synthase (2.3.3.10)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017131488A1 (ko) * | 2016-01-26 | 2017-08-03 | 경상대학교산학협력단 | 이소프렌의 생산 방법 |
| JP2017148063A (ja) * | 2011-04-29 | 2017-08-31 | ダニスコ・ユーエス・インク | メバロン酸、イソプレン及びイソプレノイドの生産量を増加させるための組み換え微生物 |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2737223A1 (en) * | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
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| SG11201401249PA (en) | 2011-10-07 | 2014-05-29 | Danisco Us Inc | Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene |
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| DE102015103608A1 (de) * | 2015-03-11 | 2016-09-15 | Basf Se | Verfahren zur mikrobiellen de novo Synthese von Terpenen |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009207376A (ja) * | 2008-03-03 | 2009-09-17 | Kirin Holdings Co Ltd | 組換え大腸菌及びそれを用いたイソプレノイドの製造方法 |
| JP2009538601A (ja) * | 2006-05-26 | 2009-11-12 | アミリス バイオテクノロジーズ， インコーポレイテッド | イソプレノイドの産生 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19629568C1 (de) | 1996-07-15 | 1998-01-08 | Fraunhofer Ges Forschung | Verfahren zur Herstellung von Isopren |
| WO2000078935A1 (en) | 1999-06-22 | 2000-12-28 | Smithkline Beecham Corporation | Mevalonate pathway genes |
| US7172886B2 (en) | 2001-12-06 | 2007-02-06 | The Regents Of The University Of California | Biosynthesis of isopentenyl pyrophosphate |
| US20050287655A1 (en) | 2002-05-10 | 2005-12-29 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing mevalonic acid |
| AU2003287028B2 (en) | 2002-10-04 | 2008-09-04 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
| RU2004124226A (ru) | 2004-08-10 | 2006-01-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Использование фосфокетолазы для продукции полезных метаболитов |
| CN101484584B (zh) * | 2006-05-26 | 2013-03-27 | 阿米瑞斯公司 | 类异戊二烯的生产 |
| US7541173B2 (en) | 2006-06-15 | 2009-06-02 | E.I. Du Pont De Nemours And Company | Solvent tolerant microorganisms and methods of isolation |
| US7947478B2 (en) | 2006-06-29 | 2011-05-24 | The Regents Of The University Of California | Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria |
| BRPI0823506A8 (pt) | 2007-12-13 | 2018-05-15 | Danisco Us Inc Genencor Div | composições e métodos para a produção de isoprene |
| WO2009100231A2 (en) | 2008-02-06 | 2009-08-13 | The Regents Of The University Of California | Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi |
| JP2011518564A (ja) | 2008-04-23 | 2011-06-30 | ダニスコ・ユーエス・インク | 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体 |
| US7632995B2 (en) | 2008-05-09 | 2009-12-15 | Monsanto Technology Llc | Plants and seeds of hybrid corn variety CH852179 |
| EP2310490B1 (en) | 2008-07-02 | 2019-01-02 | Danisco US Inc. | Methods for producing isoprene |
| HU3516U (en) | 2008-07-31 | 2008-11-28 | Zoltan Kuthi | Anti-theft protection of draining for fuel tanks |
| WO2010031079A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Systems using cell culture for production of isoprene |
| BRPI0918936A2 (pt) | 2008-09-15 | 2019-09-24 | Danisco Us Inc | conversão de derivados de prenila em isopreno |
| CA2737223A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| MY156562A (en) | 2008-09-15 | 2016-02-26 | Danisco Us Inc | Increased isoprene production using the archaeal lower mevalonate pathway |
| CA2737082A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using mevalonate kinase and isoprene synthase |
| BRPI0913680A2 (pt) | 2008-09-29 | 2019-09-24 | Butamax Advanced Biofuels Llc | "célula bacteriana ácido-láctica, método para aumentar a atividade de um polipeptídeo dihidroxi-ácido desidratase heterólogo em uma célula bacteriana ácido-láctica e método de produção de insobutanol" |
| CN102333866B (zh) | 2008-12-30 | 2015-04-29 | 丹尼斯科美国公司 | 生产异戊二烯和共同产物的方法 |
| US20100297749A1 (en) | 2009-04-21 | 2010-11-25 | Sapphire Energy, Inc. | Methods and systems for biofuel production |
| EP2421966A2 (en) | 2009-04-23 | 2012-02-29 | Danisco US Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
| TWI434921B (zh) | 2009-06-17 | 2014-04-21 | Danisco Us Inc | 從生物異戊二烯組合物製造燃料成分之方法及系統 |
| TW201412988A (zh) | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造 |
| CA2773675A1 (en) | 2009-09-15 | 2011-03-24 | Sapphire Energy, Inc. | A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
| IN2012DN02396A (enExample) | 2009-09-29 | 2015-08-21 | Butamax Tm Advanced Biofuels | |
| US20110244536A1 (en) | 2009-09-29 | 2011-10-06 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of isobutanol using highly effective ketol-acid reductoisomerase enzymes |
| CN102884028B (zh) | 2009-12-18 | 2015-08-19 | 丹尼斯科美国公司 | 从可再生资源中纯化异戊二烯 |
| AU2010336342B2 (en) | 2009-12-23 | 2015-02-26 | Danisco Us Inc. | Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (PGL) |
| JP2014519811A (ja) | 2011-04-29 | 2014-08-21 | ダニスコ・ユーエス・インク | チオラーゼ、ＨＭＧ−ＣｏＡ合成酵素及びＨＭＧ−ＣｏＡ還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産 |
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2012
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- 2012-04-27 WO PCT/US2012/035655 patent/WO2012149469A1/en not_active Ceased
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009538601A (ja) * | 2006-05-26 | 2009-11-12 | アミリス バイオテクノロジーズ， インコーポレイテッド | イソプレノイドの産生 |
| JP2009207376A (ja) * | 2008-03-03 | 2009-09-17 | Kirin Holdings Co Ltd | 組換え大腸菌及びそれを用いたイソプレノイドの製造方法 |
Non-Patent Citations (3)
| Title |
|---|
| "acetyl-CoA acetyltransferase [Listeria grayi DSM 20601]", NCBI, [ONLINE], ACCESSION NO.EFI85313, 29-JUN-2010, vol. ［検索日:2016年4月15日］, JPN6016015020, ISSN: 0003302688 * |
| "hydroxymethylglutaryl-CoA synthase [Listeria grayi DSM 20601]", NCBI, [ONLINE], ACCESSION NO.EFI85314, 29-JUN-2010, vol. ［検索日:2016年4月15日］, JPN6016015021, ISSN: 0003302689 * |
| COMPREHENSIVE NATURAL PRODUCTS II, CHEMISTRY AND BIOLOGY, 2010, PP.493-516, JPN6016015022, ISSN: 0003302690 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017148063A (ja) * | 2011-04-29 | 2017-08-31 | ダニスコ・ユーエス・インク | メバロン酸、イソプレン及びイソプレノイドの生産量を増加させるための組み換え微生物 |
| WO2017131488A1 (ko) * | 2016-01-26 | 2017-08-03 | 경상대학교산학협력단 | 이소프렌의 생산 방법 |
Also Published As
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| CN103764814B (zh) | 2016-11-16 |
| US20120276603A1 (en) | 2012-11-01 |
| US20180002727A1 (en) | 2018-01-04 |
| EP2702139A1 (en) | 2014-03-05 |
| WO2012149469A1 (en) | 2012-11-01 |
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| CN103764814A (zh) | 2014-04-30 |
| US20150037861A1 (en) | 2015-02-05 |
| BR112013027553A2 (pt) | 2019-09-24 |
| US10364443B2 (en) | 2019-07-30 |
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