CN103764814B - 利用编码具有硫解酶、hmg-coa合酶和hmg-coa还原酶酶活性的多肽的基因产生甲羟戊酸、异戊二烯和类异戊二烯 - Google Patents

利用编码具有硫解酶、hmg-coa合酶和hmg-coa还原酶酶活性的多肽的基因产生甲羟戊酸、异戊二烯和类异戊二烯 Download PDF

Info

Publication number
CN103764814B
CN103764814B CN201280031905.4A CN201280031905A CN103764814B CN 103764814 B CN103764814 B CN 103764814B CN 201280031905 A CN201280031905 A CN 201280031905A CN 103764814 B CN103764814 B CN 103764814B
Authority
CN
China
Prior art keywords
cell
gene
polypeptide
bacillus
mvas
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201280031905.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN103764814A (zh
Inventor
Z·Q·贝克
M·C·米勒
C·M·派瑞斯
Y·A·普里马克
J·P·普奇
D·H·韦尔斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Goodyear Tire and Rubber Co
Danisco USA Inc
Original Assignee
Goodyear Tire and Rubber Co
Danisco USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Goodyear Tire and Rubber Co, Danisco USA Inc filed Critical Goodyear Tire and Rubber Co
Publication of CN103764814A publication Critical patent/CN103764814A/zh
Application granted granted Critical
Publication of CN103764814B publication Critical patent/CN103764814B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01034—Hydroxymethylglutaryl-CoA reductase (NADPH) (1.1.1.34)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01016—Acetyl-CoA C-acyltransferase (2.3.1.16)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/0301—Hydroxymethylglutaryl-CoA synthase (2.3.3.10)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201280031905.4A 2011-04-29 2012-04-27 利用编码具有硫解酶、hmg-coa合酶和hmg-coa还原酶酶活性的多肽的基因产生甲羟戊酸、异戊二烯和类异戊二烯 Expired - Fee Related CN103764814B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161481098P 2011-04-29 2011-04-29
US61/481,098 2011-04-29
PCT/US2012/035655 WO2012149469A1 (en) 2011-04-29 2012-04-27 Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities

Publications (2)

Publication Number Publication Date
CN103764814A CN103764814A (zh) 2014-04-30
CN103764814B true CN103764814B (zh) 2016-11-16

Family

ID=46027009

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280031905.4A Expired - Fee Related CN103764814B (zh) 2011-04-29 2012-04-27 利用编码具有硫解酶、hmg-coa合酶和hmg-coa还原酶酶活性的多肽的基因产生甲羟戊酸、异戊二烯和类异戊二烯

Country Status (10)

Country Link
US (3) US8889383B2 (enExample)
EP (1) EP2702139A1 (enExample)
JP (1) JP2014519811A (enExample)
CN (1) CN103764814B (enExample)
AU (1) AU2012249389A1 (enExample)
BR (1) BR112013027553A2 (enExample)
CA (1) CA2834379A1 (enExample)
HK (1) HK1197266A1 (enExample)
SG (1) SG194691A1 (enExample)
WO (1) WO2012149469A1 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2737223A1 (en) * 2008-09-15 2010-03-18 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
US9121038B2 (en) * 2011-04-29 2015-09-01 The Goodyear Tire & Rubber Company Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
JP2014519811A (ja) 2011-04-29 2014-08-21 ダニスコ・ユーエス・インク チオラーゼ、ＨＭＧ−ＣｏＡ合成酵素及びＨＭＧ−ＣｏＡ還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産
SG11201401249PA (en) 2011-10-07 2014-05-29 Danisco Us Inc Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
WO2013181647A2 (en) * 2012-06-01 2013-12-05 Danisco Us Inc. Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms
ES2735331T3 (es) 2013-04-10 2019-12-18 Goodyear Tire & Rubber Fosfocetolasas para mejorar la producción de metabolitos derivados de acetil-coenzima A, isopreno, precursores de isoprenoides e isoprenoides
CN104178443B (zh) * 2013-05-24 2017-04-12 中国科学院天津工业生物技术研究所 生产丁二酸的重组大肠杆菌及其应用
MY203868A (en) 2014-06-11 2024-07-22 Univ Duke Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
CN104164457B (zh) * 2014-07-15 2016-05-04 青岛农业大学 一种利用新mva途径合成异戊二烯的方法及其相应重组细胞和应用
DE102015103608A1 (de) * 2015-03-11 2016-09-15 Basf Se Verfahren zur mikrobiellen de novo Synthese von Terpenen
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
KR101782891B1 (ko) * 2015-07-08 2017-09-29 한국과학기술연구원 미어신 생산용 벡터 및 균주, 및 이를 이용한 미어신의 생산방법
CN106544348B (zh) * 2015-09-23 2020-03-31 中国科学院微生物研究所 异戊烯基焦磷酸异构酶基因及其应用
KR101936825B1 (ko) 2016-01-26 2019-01-11 경상대학교산학협력단 이소프렌의 생산 방법
WO2017131488A1 (ko) * 2016-01-26 2017-08-03 경상대학교산학협력단 이소프렌의 생산 방법
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CA3047268A1 (en) * 2016-12-16 2018-06-21 Danisco Us Inc. Bifunctional phosphoketolase-phosphotransacetylase fusion polypeptides
US10509601B2 (en) * 2016-12-28 2019-12-17 Amazon Technologies, Inc. Data storage system with multi-tier control plane
CN107418978B (zh) * 2017-06-24 2020-10-23 天津大学 一种利用生物柴油副产物制备金合欢烯的方法
CN109234216B (zh) * 2017-07-10 2022-05-20 上海医药工业研究院 一种生产鲨烯的基因工程菌及其方法
CN107841523A (zh) * 2017-10-19 2018-03-27 浙江大学 利用群体感应分子诱导从桦褐孔菌中提取三萜类物质方法
CN114634888B (zh) * 2022-03-04 2023-05-26 中南大学 一种铊氧化菌、微生物菌剂及其应用
AU2023289395A1 (en) 2022-06-21 2025-01-09 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates
WO2024263850A1 (en) * 2023-06-21 2024-12-26 Lanzatech, Inc. Engineered autotrophic microorganisms for isoprenoid production and methods
CN117025462B (zh) * 2023-08-04 2024-02-23 西湖大学 一种降解杀虫剂的肠球菌菌株及其用途
CN117925653A (zh) * 2024-03-13 2024-04-26 扬州大学 黄瓜CsHMGR1基因及其抗枯萎病的应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000078935A1 (en) * 1999-06-22 2000-12-28 Smithkline Beecham Corporation Mevalonate pathway genes
EP1510583A1 (en) * 2002-05-10 2005-03-02 Kyowa Hakko Kogyo Co., Ltd. Process for producing mevalonic acid
WO2007140339A2 (en) * 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Production of isoprenoids
WO2009076676A2 (en) * 2007-12-13 2009-06-18 Danisco Us Inc. Compositions and methods for producing isoprene
CN101484584A (zh) * 2006-05-26 2009-07-15 阿米瑞斯生物技术公司 类异戊二烯的生产
WO2010078457A2 (en) * 2008-12-30 2010-07-08 Danisco Us Inc. Methods of producing isoprene and a co-product

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
RU2004124226A (ru) 2004-08-10 2006-01-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Использование фосфокетолазы для продукции полезных метаболитов
US7541173B2 (en) 2006-06-15 2009-06-02 E.I. Du Pont De Nemours And Company Solvent tolerant microorganisms and methods of isolation
US7947478B2 (en) 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
WO2009100231A2 (en) 2008-02-06 2009-08-13 The Regents Of The University Of California Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi
JP5405030B2 (ja) * 2008-03-03 2014-02-05 キリンホールディングス株式会社 組換え大腸菌及びそれを用いたイソプレノイドの製造方法
JP2011518564A (ja) 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体
US7632995B2 (en) 2008-05-09 2009-12-15 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH852179
EP2310490B1 (en) 2008-07-02 2019-01-02 Danisco US Inc. Methods for producing isoprene
HU3516U (en) 2008-07-31 2008-11-28 Zoltan Kuthi Anti-theft protection of draining for fuel tanks
WO2010031079A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Systems using cell culture for production of isoprene
BRPI0918936A2 (pt) 2008-09-15 2019-09-24 Danisco Us Inc conversão de derivados de prenila em isopreno
CA2737223A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
MY156562A (en) 2008-09-15 2016-02-26 Danisco Us Inc Increased isoprene production using the archaeal lower mevalonate pathway
CA2737082A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
BRPI0913680A2 (pt) 2008-09-29 2019-09-24 Butamax Advanced Biofuels Llc "célula bacteriana ácido-láctica, método para aumentar a atividade de um polipeptídeo dihidroxi-ácido desidratase heterólogo em uma célula bacteriana ácido-láctica e método de produção de insobutanol"
US20100297749A1 (en) 2009-04-21 2010-11-25 Sapphire Energy, Inc. Methods and systems for biofuel production
EP2421966A2 (en) 2009-04-23 2012-02-29 Danisco US Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
TWI434921B (zh) 2009-06-17 2014-04-21 Danisco Us Inc 從生物異戊二烯組合物製造燃料成分之方法及系統
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
CA2773675A1 (en) 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
IN2012DN02396A (enExample) 2009-09-29 2015-08-21 Butamax Tm Advanced Biofuels
US20110244536A1 (en) 2009-09-29 2011-10-06 Butamax(Tm) Advanced Biofuels Llc Fermentive production of isobutanol using highly effective ketol-acid reductoisomerase enzymes
CN102884028B (zh) 2009-12-18 2015-08-19 丹尼斯科美国公司 从可再生资源中纯化异戊二烯
AU2010336342B2 (en) 2009-12-23 2015-02-26 Danisco Us Inc. Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (PGL)
JP2014519811A (ja) 2011-04-29 2014-08-21 ダニスコ・ユーエス・インク チオラーゼ、ＨＭＧ−ＣｏＡ合成酵素及びＨＭＧ−ＣｏＡ還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000078935A1 (en) * 1999-06-22 2000-12-28 Smithkline Beecham Corporation Mevalonate pathway genes
EP1510583A1 (en) * 2002-05-10 2005-03-02 Kyowa Hakko Kogyo Co., Ltd. Process for producing mevalonic acid
WO2007140339A2 (en) * 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Production of isoprenoids
CN101484584A (zh) * 2006-05-26 2009-07-15 阿米瑞斯生物技术公司 类异戊二烯的生产
WO2009076676A2 (en) * 2007-12-13 2009-06-18 Danisco Us Inc. Compositions and methods for producing isoprene
CN102027124A (zh) * 2007-12-13 2011-04-20 丹尼斯科美国公司 用于生产异戊二烯的组合物和方法
WO2010078457A2 (en) * 2008-12-30 2010-07-08 Danisco Us Inc. Methods of producing isoprene and a co-product

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli;Douglas J. Pitera et al.;《Metabolic Engineering》;20061123;第193-207页 *
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids;Vincent JJ Martin et al.;《NATURE BIOTECHNOLOGY》;20030731;第21卷(第7期);第796-802页 *

Also Published As

Publication number Publication date
US20120276603A1 (en) 2012-11-01
US20180002727A1 (en) 2018-01-04
EP2702139A1 (en) 2014-03-05
WO2012149469A1 (en) 2012-11-01
US8889383B2 (en) 2014-11-18
HK1197266A1 (en) 2015-01-09
AU2012249389A1 (en) 2013-11-14
CA2834379A1 (en) 2012-11-01
US9752162B2 (en) 2017-09-05
SG194691A1 (en) 2013-12-30
CN103764814A (zh) 2014-04-30
JP2014519811A (ja) 2014-08-21
US20150037861A1 (en) 2015-02-05
BR112013027553A2 (pt) 2019-09-24
US10364443B2 (en) 2019-07-30

Similar Documents

Publication Publication Date Title
CN103764814B (zh) 利用编码具有硫解酶、hmg-coa合酶和hmg-coa还原酶酶活性的多肽的基因产生甲羟戊酸、异戊二烯和类异戊二烯
US10975394B2 (en) Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
US10113185B2 (en) Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
US11371035B2 (en) Phosphoketolases for improved production of acetyl coenzyme A-derived metabolites, isoprene, isoprenoid precursors, and isoprenoid
US20130122562A1 (en) Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1197266

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20161116

Termination date: 20170427

REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1197266

Country of ref document: HK