JP2014511704A - Ｔ細胞のプライミングのための方法 - Google Patents
Ｔ細胞のプライミングのための方法 Download PDFInfo
- Publication number
- JP2014511704A JP2014511704A JP2014504317A JP2014504317A JP2014511704A JP 2014511704 A JP2014511704 A JP 2014511704A JP 2014504317 A JP2014504317 A JP 2014504317A JP 2014504317 A JP2014504317 A JP 2014504317A JP 2014511704 A JP2014511704 A JP 2014511704A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- antigen
- specific
- viral
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 210000001744 T-lymphocyte Anatomy 0.000 title claims abstract description 124
- 238000000034 method Methods 0.000 title claims abstract description 68
- 230000037452 priming Effects 0.000 title claims abstract description 13
- 239000000203 mixture Substances 0.000 claims abstract description 28
- 208000036142 Viral infection Diseases 0.000 claims abstract description 27
- 230000009385 viral infection Effects 0.000 claims abstract description 27
- 238000000338 in vitro Methods 0.000 claims abstract description 11
- 210000004027 cell Anatomy 0.000 claims description 94
- 239000000427 antigen Substances 0.000 claims description 70
- 108091007433 antigens Proteins 0.000 claims description 70
- 102000036639 antigens Human genes 0.000 claims description 70
- 210000004443 dendritic cell Anatomy 0.000 claims description 55
- 230000000735 allogeneic effect Effects 0.000 claims description 54
- 210000004698 lymphocyte Anatomy 0.000 claims description 53
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims description 47
- 241000700605 Viruses Species 0.000 claims description 34
- 102100027207 CD27 antigen Human genes 0.000 claims description 33
- 101000914511 Homo sapiens CD27 antigen Proteins 0.000 claims description 33
- 210000001616 monocyte Anatomy 0.000 claims description 33
- 210000000612 antigen-presenting cell Anatomy 0.000 claims description 29
- 239000000463 material Substances 0.000 claims description 17
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 17
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 230000003612 virological effect Effects 0.000 claims description 17
- 238000012258 culturing Methods 0.000 claims description 15
- 108010074328 Interferon-gamma Proteins 0.000 claims description 13
- 239000003446 ligand Substances 0.000 claims description 13
- 102000004169 proteins and genes Human genes 0.000 claims description 13
- 208000015181 infectious disease Diseases 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 12
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 11
- 230000001684 chronic effect Effects 0.000 claims description 10
- 108010002350 Interleukin-2 Proteins 0.000 claims description 9
- 230000006907 apoptotic process Effects 0.000 claims description 9
- 210000000265 leukocyte Anatomy 0.000 claims description 9
- 108010067390 Viral Proteins Proteins 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 101000831496 Homo sapiens Toll-like receptor 3 Proteins 0.000 claims description 6
- 102000043129 MHC class I family Human genes 0.000 claims description 6
- 108091054437 MHC class I family Proteins 0.000 claims description 6
- 102100024324 Toll-like receptor 3 Human genes 0.000 claims description 6
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims description 6
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 229960004854 viral vaccine Drugs 0.000 claims description 6
- 108090000672 Annexin A5 Proteins 0.000 claims description 5
- 102000004121 Annexin A5 Human genes 0.000 claims description 5
- -1 IL-1β Proteins 0.000 claims description 5
- 102000043131 MHC class II family Human genes 0.000 claims description 5
- 108091054438 MHC class II family Proteins 0.000 claims description 5
- 206010070834 Sensitisation Diseases 0.000 claims description 5
- 230000008313 sensitization Effects 0.000 claims description 5
- 230000001225 therapeutic effect Effects 0.000 claims description 5
- 101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 claims description 4
- 102000008070 Interferon-gamma Human genes 0.000 claims description 4
- 108090000172 Interleukin-15 Proteins 0.000 claims description 4
- 108091036414 Polyinosinic:polycytidylic acid Proteins 0.000 claims description 4
- 102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 claims description 4
- 230000002458 infectious effect Effects 0.000 claims description 4
- 229960003130 interferon gamma Drugs 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 238000001890 transfection Methods 0.000 claims description 4
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 claims description 3
- 101000669447 Homo sapiens Toll-like receptor 4 Proteins 0.000 claims description 3
- 101000669402 Homo sapiens Toll-like receptor 7 Proteins 0.000 claims description 3
- 102000006992 Interferon-alpha Human genes 0.000 claims description 3
- 108010047761 Interferon-alpha Proteins 0.000 claims description 3
- 108090000978 Interleukin-4 Proteins 0.000 claims description 3
- 108010002586 Interleukin-7 Proteins 0.000 claims description 3
- 102100039360 Toll-like receptor 4 Human genes 0.000 claims description 3
- 102100039390 Toll-like receptor 7 Human genes 0.000 claims description 3
- 230000007416 antiviral immune response Effects 0.000 claims description 3
- 108090000467 Interferon-beta Proteins 0.000 claims description 2
- 102000003996 Interferon-beta Human genes 0.000 claims description 2
- 108020004999 messenger RNA Proteins 0.000 claims description 2
- 229940124614 TLR 8 agonist Drugs 0.000 claims 2
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 claims 1
- 229940044616 toll-like receptor 7 agonist Drugs 0.000 claims 1
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 24
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 24
- 241000725303 Human immunodeficiency virus Species 0.000 description 19
- 230000004083 survival effect Effects 0.000 description 13
- 230000035755 proliferation Effects 0.000 description 12
- 101710191958 Amino-acid acetyltransferase Proteins 0.000 description 11
- 102100023167 Argininosuccinate lyase Human genes 0.000 description 11
- 230000028993 immune response Effects 0.000 description 11
- 210000004369 blood Anatomy 0.000 description 10
- 239000008280 blood Substances 0.000 description 10
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 230000004044 response Effects 0.000 description 10
- 102100037850 Interferon gamma Human genes 0.000 description 9
- 108091008874 T cell receptors Proteins 0.000 description 9
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 9
- 210000003719 b-lymphocyte Anatomy 0.000 description 9
- 210000000987 immune system Anatomy 0.000 description 9
- 230000002062 proliferating effect Effects 0.000 description 9
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 8
- 238000001994 activation Methods 0.000 description 8
- 230000000638 stimulation Effects 0.000 description 8
- 241000701022 Cytomegalovirus Species 0.000 description 7
- 102000000588 Interleukin-2 Human genes 0.000 description 7
- 230000004913 activation Effects 0.000 description 7
- 230000000139 costimulatory effect Effects 0.000 description 7
- 239000012636 effector Substances 0.000 description 7
- 230000002163 immunogen Effects 0.000 description 7
- 230000000670 limiting effect Effects 0.000 description 7
- 230000006044 T cell activation Effects 0.000 description 6
- 238000009169 immunotherapy Methods 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- 241000282412 Homo Species 0.000 description 5
- 208000037581 Persistent Infection Diseases 0.000 description 5
- 210000003162 effector t lymphocyte Anatomy 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 239000013603 viral vector Substances 0.000 description 5
- 102000004127 Cytokines Human genes 0.000 description 4
- 108090000695 Cytokines Proteins 0.000 description 4
- 241000711549 Hepacivirus C Species 0.000 description 4
- 241000712431 Influenza A virus Species 0.000 description 4
- 230000005867 T cell response Effects 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 210000005259 peripheral blood Anatomy 0.000 description 4
- 239000011886 peripheral blood Substances 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 238000002255 vaccination Methods 0.000 description 4
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 3
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 description 3
- 241000700721 Hepatitis B virus Species 0.000 description 3
- 102000004388 Interleukin-4 Human genes 0.000 description 3
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000030741 antigen processing and presentation Effects 0.000 description 3
- 210000001185 bone marrow Anatomy 0.000 description 3
- 238000003501 co-culture Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000001472 cytotoxic effect Effects 0.000 description 3
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000035800 maturation Effects 0.000 description 3
- 210000000581 natural killer T-cell Anatomy 0.000 description 3
- 210000000822 natural killer cell Anatomy 0.000 description 3
- 230000002085 persistent effect Effects 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 230000003827 upregulation Effects 0.000 description 3
- 229960005486 vaccine Drugs 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- 238000010600 3H thymidine incorporation assay Methods 0.000 description 2
- 208000030507 AIDS Diseases 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 102100036301 C-C chemokine receptor type 7 Human genes 0.000 description 2
- 101150013553 CD40 gene Proteins 0.000 description 2
- 102100035793 CD83 antigen Human genes 0.000 description 2
- 241000724252 Cucumber mosaic virus Species 0.000 description 2
- 206010061818 Disease progression Diseases 0.000 description 2
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 description 2
- 208000031886 HIV Infections Diseases 0.000 description 2
- 208000037357 HIV infectious disease Diseases 0.000 description 2
- 102100036242 HLA class II histocompatibility antigen, DQ alpha 2 chain Human genes 0.000 description 2
- 102000008949 Histocompatibility Antigens Class I Human genes 0.000 description 2
- 108010088652 Histocompatibility Antigens Class I Proteins 0.000 description 2
- 101000716065 Homo sapiens C-C chemokine receptor type 7 Proteins 0.000 description 2
- 101000946856 Homo sapiens CD83 antigen Proteins 0.000 description 2
- 101000930801 Homo sapiens HLA class II histocompatibility antigen, DQ alpha 2 chain Proteins 0.000 description 2
- 102000013462 Interleukin-12 Human genes 0.000 description 2
- 108010065805 Interleukin-12 Proteins 0.000 description 2
- 102000003979 Mineralocorticoid Receptors Human genes 0.000 description 2
- 108090000375 Mineralocorticoid Receptors Proteins 0.000 description 2
- 210000000447 Th1 cell Anatomy 0.000 description 2
- 102000008235 Toll-Like Receptor 9 Human genes 0.000 description 2
- 108010060818 Toll-Like Receptor 9 Proteins 0.000 description 2
- 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 238000011467 adoptive cell therapy Methods 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 230000000890 antigenic effect Effects 0.000 description 2
- 239000006143 cell culture medium Substances 0.000 description 2
- 230000006369 cell cycle progression Effects 0.000 description 2
- 230000001461 cytolytic effect Effects 0.000 description 2
- 231100000433 cytotoxic Toxicity 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000005750 disease progression Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000004073 interleukin-2 production Effects 0.000 description 2
- 238000001990 intravenous administration Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 210000001165 lymph node Anatomy 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229960004857 mitomycin Drugs 0.000 description 2
- OHDXDNUPVVYWOV-UHFFFAOYSA-N n-methyl-1-(2-naphthalen-1-ylsulfanylphenyl)methanamine Chemical compound CNCC1=CC=CC=C1SC1=CC=CC2=CC=CC=C12 OHDXDNUPVVYWOV-UHFFFAOYSA-N 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000009877 rendering Methods 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 102000006306 Antigen Receptors Human genes 0.000 description 1
- 108010083359 Antigen Receptors Proteins 0.000 description 1
- 210000001239 CD8-positive, alpha-beta cytotoxic T lymphocyte Anatomy 0.000 description 1
- 102000000844 Cell Surface Receptors Human genes 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010011831 Cytomegalovirus infection Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 1
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 1
- 102000011786 HLA-A Antigens Human genes 0.000 description 1
- 108010075704 HLA-A Antigens Proteins 0.000 description 1
- 102000025850 HLA-A2 Antigen Human genes 0.000 description 1
- 108010074032 HLA-A2 Antigen Proteins 0.000 description 1
- 102000015789 HLA-DP Antigens Human genes 0.000 description 1
- 108010010378 HLA-DP Antigens Proteins 0.000 description 1
- 101000746373 Homo sapiens Granulocyte-macrophage colony-stimulating factor Proteins 0.000 description 1
- 101000599940 Homo sapiens Interferon gamma Proteins 0.000 description 1
- 101001002709 Homo sapiens Interleukin-4 Proteins 0.000 description 1
- 101000914484 Homo sapiens T-lymphocyte activation antigen CD80 Proteins 0.000 description 1
- 101000800483 Homo sapiens Toll-like receptor 8 Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229930192392 Mitomycin Natural products 0.000 description 1
- 108010067902 Peptide Library Proteins 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 102100027222 T-lymphocyte activation antigen CD80 Human genes 0.000 description 1
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 1
- 102100033110 Toll-like receptor 8 Human genes 0.000 description 1
- 206010052779 Transplant rejections Diseases 0.000 description 1
- 102000008579 Transposases Human genes 0.000 description 1
- 108010020764 Transposases Proteins 0.000 description 1
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002424 anti-apoptotic effect Effects 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 230000001028 anti-proliverative effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000001640 apoptogenic effect Effects 0.000 description 1
- 230000003305 autocrine Effects 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 102000015736 beta 2-Microglobulin Human genes 0.000 description 1
- 108010081355 beta 2-Microglobulin Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000009172 cell transfer therapy Methods 0.000 description 1
- 229940030156 cell vaccine Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000003399 chemotactic effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000005860 defense response to virus Effects 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000012202 endocytosis Effects 0.000 description 1
- 230000002964 excitative effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 230000005745 host immune response Effects 0.000 description 1
- 102000046157 human CSF2 Human genes 0.000 description 1
- 102000043557 human IFNG Human genes 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000010874 in vitro model Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229960001388 interferon-beta Drugs 0.000 description 1
- 230000019734 interleukin-12 production Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 210000004324 lymphatic system Anatomy 0.000 description 1
- 210000000207 lymphocyte subset Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000013586 microbial product Substances 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000007799 mixed lymphocyte reaction assay Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000009696 proliferative response Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000008593 response to virus Effects 0.000 description 1
- 230000004043 responsiveness Effects 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000004017 serum-free culture medium Substances 0.000 description 1
- 230000036332 sexual response Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- YEENEYXBHNNNGV-XEHWZWQGSA-M sodium;3-acetamido-5-[acetyl(methyl)amino]-2,4,6-triiodobenzoate;(2r,3r,4s,5s,6r)-2-[(2r,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound [Na+].CC(=O)N(C)C1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I.O[C@H]1[C@H](O)[C@@H](CO)O[C@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 YEENEYXBHNNNGV-XEHWZWQGSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000007474 system interaction Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 102000003298 tumor necrosis factor receptor Human genes 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/145—Orthomyxoviridae, e.g. influenza virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/10—Cellular immunotherapy characterised by the cell type used
- A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
- A61K40/46—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
- C12N2502/1121—Dendritic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16211—Lymphocryptovirus, e.g. human herpesvirus 4, Epstein-Barr Virus
- C12N2710/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pulmonology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Developmental Biology & Embryology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161474892P | 2011-04-13 | 2011-04-13 | |
| US61/474,892 | 2011-04-13 | ||
| PCT/EP2012/056658 WO2012140127A2 (en) | 2011-04-13 | 2012-04-12 | Method for priming of t cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014511704A true JP2014511704A (ja) | 2014-05-19 |
| JP2014511704A5 JP2014511704A5 (enExample) | 2015-05-28 |
Family
ID=45953145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014504317A Withdrawn JP2014511704A (ja) | 2011-04-13 | 2012-04-12 | Ｔ細胞のプライミングのための方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9492529B2 (enExample) |
| EP (1) | EP2696894B1 (enExample) |
| JP (1) | JP2014511704A (enExample) |
| KR (1) | KR20140043340A (enExample) |
| CN (1) | CN103533955A (enExample) |
| WO (1) | WO2012140127A2 (enExample) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018511320A (ja) * | 2015-03-26 | 2018-04-26 | ブライアン ジェイ ツェルニキＣＺＥＲＮＩＥＣＫＩ， Ｂｒｉａｎ， Ｊ． | 治療およびエピトープマッピング用Ｔ細胞の感作および増殖のためのｉｎ ｖｉｔｒｏ人工リンパ節法 |
| JP2019500604A (ja) * | 2015-12-16 | 2019-01-10 | テーセーエーエル アーベー | Ｔ細胞反応性プラットフォーム |
| WO2019069561A1 (ja) * | 2017-10-03 | 2019-04-11 | 公益財団法人東京都医学総合研究所 | インフルエンザに対する医薬 |
| JP2022028677A (ja) * | 2016-07-08 | 2022-02-16 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | Ｈｉｖ予備免疫化および免疫療法 |
| US12090200B2 (en) | 2016-02-08 | 2024-09-17 | American Gene Technologies International Inc. | Methods of producing cells resistant to HIV infection |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2591230T3 (es) * | 2012-12-18 | 2016-11-25 | Immunicum Ab | Co-diferenciación y activación de monocitos procedentes de donantes alogénicos para producir células dendríticas pro-inflamatorias |
| US20150320799A1 (en) | 2012-12-20 | 2015-11-12 | Purdue Research Foundation | Chimeric antigen receptor-expressing t cells as anti-cancer therapeutics |
| CN107532174A (zh) | 2015-03-11 | 2018-01-02 | 得克萨斯州大学系统董事会 | 转座酶多肽及其用途 |
| WO2016154508A1 (en) * | 2015-03-26 | 2016-09-29 | The Trustees Of The University Of Pennsylvania | In vitro artificial lymph node for sensitization and expansion of t cells for therapy and epitope mapping |
| AU2016259020B2 (en) * | 2015-05-07 | 2021-12-09 | Baylor College Of Medicine | Dendritic cell immunotherapy |
| CN105543170A (zh) * | 2015-12-31 | 2016-05-04 | 中山大学 | 一种可刺激t细胞扩增的组合物 |
| JP7282521B2 (ja) | 2016-04-08 | 2023-05-29 | パーデュー・リサーチ・ファウンデイション | Ｃａｒ ｔ細胞療法のための方法および組成物 |
| EP3579870A4 (en) | 2017-02-07 | 2020-12-30 | Seattle Children's Hospital (DBA Seattle Children's Research Institute) | PHOSPHOLIPID ETHER (PLE) T CAR-LYMPHOCYTE TUMOR (CTCT) TARGETING AGENTS |
| WO2018160622A1 (en) | 2017-02-28 | 2018-09-07 | Endocyte, Inc. | Compositions and methods for car t cell therapy |
| AU2019209432A1 (en) | 2018-01-22 | 2020-08-06 | Seattle Children's Hospital (dba Seattle Children's Research Institute) | Methods of use for CAR T cells |
| CN111936518A (zh) | 2018-02-06 | 2020-11-13 | 西雅图儿童医院（Dba西雅图儿童研究所） | 对fl-ple标记的肿瘤表现出最佳的t细胞功能的荧光素特异性car |
| WO2019165237A1 (en) | 2018-02-23 | 2019-08-29 | Endocyte, Inc. | Sequencing method for car t cell therapy |
| CN109370987A (zh) * | 2018-11-23 | 2019-02-22 | 见多视光(北京)科技有限公司 | 外周血ctl细胞改良的培养方法 |
| KR102234394B1 (ko) * | 2019-03-08 | 2021-03-31 | 신지섭 | 타가면역세포배양방법, 그 방법으로 얻어진 면역세포배양액 및 이를 포함하는 면역세포치료제 |
| WO2020219812A1 (en) | 2019-04-26 | 2020-10-29 | Allogene Therapeutics, Inc. | Methods of manufacturing allogeneic car t cells |
| WO2021006733A1 (en) * | 2019-07-08 | 2021-01-14 | Erasmus University Medical Center Rotterdam | Rapid in vitro t cell exhaustion |
| CA3201851A1 (en) * | 2020-12-09 | 2022-06-16 | Ryan SAADI | Virus specific t-cells and methods of treating and preventing viral infections |
| CN115877017B (zh) * | 2023-03-01 | 2023-05-05 | 首都医科大学附属北京地坛医院 | 预测hiv/aids患者免疫重建的产品和系统 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6821778B1 (en) | 1993-12-01 | 2004-11-23 | The Board Of Trustees Of Leland Stanford Junior University | Methods for using dendritic cells to activate gamma/delta-T cell receptor-positive T cells |
| US5827642A (en) | 1994-08-31 | 1998-10-27 | Fred Hutchinson Cancer Research Center | Rapid expansion method ("REM") for in vitro propagation of T lymphocytes |
| ATE428769T1 (de) | 1997-10-27 | 2009-05-15 | Univ Rockefeller | Methode und zusammensetzung zur herstellung von reifen dendritischen zellen |
| JP2008521406A (ja) | 2004-11-24 | 2008-06-26 | フレッド ハッチンソン キャンサー リサーチ センター | 養子免疫療法のためのｉｌ−２１の使用法および腫瘍抗原の同定法 |
| EP1712634A1 (en) | 2005-04-13 | 2006-10-18 | Wittycell SAS | Selection of highly efficient antigen presenting cells for regulating immunity and uses thereof |
| HUE029164T2 (hu) | 2007-09-14 | 2017-02-28 | Univ Brussel Vrije | Humán antigén-prezentáló sejtek T-sejteket stimuláló képességének fokozása és azok alkalmazása vakcinázásra |
| CN102597222B (zh) * | 2009-10-27 | 2015-07-15 | 因缪尼卡姆股份公司 | 用于增殖抗原特异性t细胞的方法 |
-
2012
- 2012-04-12 US US14/110,899 patent/US9492529B2/en not_active Expired - Fee Related
- 2012-04-12 WO PCT/EP2012/056658 patent/WO2012140127A2/en not_active Ceased
- 2012-04-12 JP JP2014504317A patent/JP2014511704A/ja not_active Withdrawn
- 2012-04-12 KR KR1020137029247A patent/KR20140043340A/ko not_active Withdrawn
- 2012-04-12 CN CN201280018223.XA patent/CN103533955A/zh active Pending
- 2012-04-12 EP EP12713992.1A patent/EP2696894B1/en not_active Not-in-force
-
2016
- 2016-11-14 US US15/350,124 patent/US10000736B2/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018511320A (ja) * | 2015-03-26 | 2018-04-26 | ブライアン ジェイ ツェルニキＣＺＥＲＮＩＥＣＫＩ， Ｂｒｉａｎ， Ｊ． | 治療およびエピトープマッピング用Ｔ細胞の感作および増殖のためのｉｎ ｖｉｔｒｏ人工リンパ節法 |
| JP2019500604A (ja) * | 2015-12-16 | 2019-01-10 | テーセーエーエル アーベー | Ｔ細胞反応性プラットフォーム |
| US12090200B2 (en) | 2016-02-08 | 2024-09-17 | American Gene Technologies International Inc. | Methods of producing cells resistant to HIV infection |
| JP2022028677A (ja) * | 2016-07-08 | 2022-02-16 | アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド | Ｈｉｖ予備免疫化および免疫療法 |
| US11911458B2 (en) | 2016-07-08 | 2024-02-27 | American Gene Technologies International Inc. | HIV pre-immunization and immunotherapy |
| US12370253B2 (en) | 2016-07-08 | 2025-07-29 | American Gene Technologies International Inc. | Pre-immunization and immunotherapy |
| WO2019069561A1 (ja) * | 2017-10-03 | 2019-04-11 | 公益財団法人東京都医学総合研究所 | インフルエンザに対する医薬 |
| CN111065400A (zh) * | 2017-10-03 | 2020-04-24 | 公益财团法人东京都医学总合研究所 | 抗流感药物 |
| JPWO2019069561A1 (ja) * | 2017-10-03 | 2020-10-22 | 公益財団法人東京都医学総合研究所 | インフルエンザに対する医薬 |
Also Published As
| Publication number | Publication date |
|---|---|
| US10000736B2 (en) | 2018-06-19 |
| WO2012140127A2 (en) | 2012-10-18 |
| US9492529B2 (en) | 2016-11-15 |
| WO2012140127A3 (en) | 2013-01-03 |
| KR20140043340A (ko) | 2014-04-09 |
| EP2696894B1 (en) | 2017-11-01 |
| CN103533955A (zh) | 2014-01-22 |
| US20170152479A1 (en) | 2017-06-01 |
| EP2696894A2 (en) | 2014-02-19 |
| US20140093538A1 (en) | 2014-04-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10000736B2 (en) | Method for priming of T cells | |
| CN102597222B (zh) | 用于增殖抗原特异性t细胞的方法 | |
| JP6081483B2 (ja) | Ｔ細胞を増殖させるプロセス | |
| KR102028340B1 (ko) | 항원-특이적 t 세포의 증식 방법 | |
| Jabbari et al. | Secondary memory CD8+ T cells are more protective but slower to acquire a central–memory phenotype | |
| CN103898051B (zh) | 提高免疫反应性的方法 | |
| JP2014511704A5 (enExample) | ||
| Tsai et al. | In vitro immunization and expansion of antigen-specific cytotoxic T lymphocytes for adoptive immunotherapy using peptide-pulsed dendritic cells | |
| JP6697076B2 (ja) | ウイルス抗原特異的ｔ細胞の濃縮および拡大方法 | |
| JP2020533977A (ja) | がん処置における使用のための改良された同種樹状細胞 | |
| US20040219168A1 (en) | Method for generating highly active human dendritic cells from monocytes | |
| JP2025504109A (ja) | Ｅｂｖ複合抗原、樹状細胞ワクチンおよびその使用 | |
| RU2465324C1 (ru) | Способ генерации специфического иммунного ответа против антигенов вируса иммунодефицита человека | |
| Chen et al. | EBV LMP2A-specific T cell immune responses elicited by dendritic cells loaded with LMP2A protein | |
| JP2023534740A (ja) | Ｅｂｖを標的とした同種異系ｂ細胞ワクチン及びその調製方法 | |
| HK1168869B (en) | Method for proliferation of antigen-specific t cells | |
| HK1168869A1 (en) | Method for proliferation of antigen-specific t cells | |
| WO2004069272A2 (en) | Adjuvant combination for use in the immunization of a mamal comprising il2 and il12 | |
| AU2002317739A1 (en) | method for generating highly active human dendritic cells from monocytes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150406 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20150406 |
|
| A761 | Written withdrawal of application |
Free format text: JAPANESE INTERMEDIATE CODE: A761 Effective date: 20150928 |