JP2014507950A5 - - Google Patents
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- Publication number
- JP2014507950A5 JP2014507950A5 JP2013556801A JP2013556801A JP2014507950A5 JP 2014507950 A5 JP2014507950 A5 JP 2014507950A5 JP 2013556801 A JP2013556801 A JP 2013556801A JP 2013556801 A JP2013556801 A JP 2013556801A JP 2014507950 A5 JP2014507950 A5 JP 2014507950A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- strand
- sequence
- target
- complementary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 34
- 108020004707 nucleic acids Proteins 0.000 claims 32
- 102000039446 nucleic acids Human genes 0.000 claims 32
- 230000000903 blocking effect Effects 0.000 claims 27
- 238000012163 sequencing technique Methods 0.000 claims 22
- 230000000295 complement effect Effects 0.000 claims 16
- 238000000034 method Methods 0.000 claims 12
- 239000011541 reaction mixture Substances 0.000 claims 12
- 125000003729 nucleotide group Chemical group 0.000 claims 7
- 230000003321 amplification Effects 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- 108091027305 Heteroduplex Proteins 0.000 claims 4
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 4
- 229910019142 PO4 Inorganic materials 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 239000010452 phosphate Substances 0.000 claims 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 238000001712 DNA sequencing Methods 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 238000004925 denaturation Methods 0.000 claims 2
- 230000036425 denaturation Effects 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 238000002844 melting Methods 0.000 claims 2
- 230000008018 melting Effects 0.000 claims 2
- 230000002438 mitochondrial effect Effects 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- 108020004705 Codon Proteins 0.000 claims 1
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 claims 1
- 101100193693 Kirsten murine sarcoma virus K-RAS gene Proteins 0.000 claims 1
- 208000035177 MELAS Diseases 0.000 claims 1
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000012165 high-throughput sequencing Methods 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 238000012175 pyrosequencing Methods 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161447490P | 2011-02-28 | 2011-02-28 | |
| US61/447,490 | 2011-02-28 | ||
| US201161532887P | 2011-09-09 | 2011-09-09 | |
| US61/532,887 | 2011-09-09 | ||
| PCT/US2012/026938 WO2012118802A1 (en) | 2011-02-28 | 2012-02-28 | Kit and method for sequencing a target dna in a mixed population |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014507950A JP2014507950A (ja) | 2014-04-03 |
| JP2014507950A5 true JP2014507950A5 (cg-RX-API-DMAC7.html) | 2015-04-16 |
Family
ID=45815993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013556801A Pending JP2014507950A (ja) | 2011-02-28 | 2012-02-28 | 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20120225421A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2681332A1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2014507950A (cg-RX-API-DMAC7.html) |
| KR (1) | KR20140010093A (cg-RX-API-DMAC7.html) |
| CN (1) | CN103517993A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2012223438A1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2828535A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2012118802A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2545189B1 (en) | 2010-03-08 | 2018-01-10 | Dana-Farber Cancer Institute, Inc. | Full cold-pcr enrichment with reference blocking sequence |
| US11130992B2 (en) | 2011-03-31 | 2021-09-28 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex COLD-PCR |
| WO2014089797A1 (zh) * | 2012-12-13 | 2014-06-19 | 深圳华大基因科技服务有限公司 | 用于高通量测序的锁核酸修饰的dna片段 |
| US10913977B2 (en) | 2013-07-24 | 2021-02-09 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR |
| KR20160003444A (ko) * | 2014-07-01 | 2016-01-11 | 주식회사 유진셀 | 핵산말단분해효소를 이용한 서열-특이적 핵산 검출 방법 및 장치와 이에 사용되는 키트 |
| CN107532203B (zh) * | 2015-03-06 | 2019-09-13 | 真固生物科技有限公司 | 重叠扩增子的选择性扩增 |
| JP2018518155A (ja) * | 2015-04-15 | 2018-07-12 | ザ ジェネラル ホスピタル コーポレイション | Ｌｎａベース突然変異体エンリッチメント次世代シーケンシングアッセイ |
| WO2016172265A1 (en) * | 2015-04-20 | 2016-10-27 | Neogenomics Laboratories, Inc. | Method to increase sensitivity of next generation sequencing |
| WO2017070339A1 (en) * | 2015-10-20 | 2017-04-27 | Richardson Katherine | Microfluidic device for enrichment of nucleic acid sequence alterations |
| WO2018111835A1 (en) | 2016-12-12 | 2018-06-21 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
| WO2019023243A1 (en) | 2017-07-24 | 2019-01-31 | Dana-Farber Cancer Institute, Inc. | METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE |
| CN115927566B (zh) * | 2017-09-20 | 2025-02-18 | 深圳华大智造科技股份有限公司 | 一种用于Small RNA的测序方法、测序试剂和应用 |
| CN114096679B (zh) * | 2019-07-11 | 2024-04-09 | 学校法人东京理科大学 | 使用固相载体的核酸扩增方法 |
| CN113567404A (zh) * | 2021-06-11 | 2021-10-29 | 上海交通大学 | 一种分析肿瘤细胞耐药性的方法和试剂盒 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2293238A (en) * | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
| US5849497A (en) * | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
| FR2779154B1 (fr) * | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
| US6045993A (en) * | 1998-05-30 | 2000-04-04 | Visible Genetics Inc. | Method, reagent and kit for genotyping of human papillomavirus |
| DE10012540B4 (de) * | 2000-03-15 | 2004-09-23 | Vermicon Ag | Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion |
| JP2004520812A (ja) * | 2000-08-30 | 2004-07-15 | ハプロゲン・エルエルシー | 対立遺伝子を決定するための方法 |
| GB0406863D0 (en) * | 2004-03-26 | 2004-04-28 | Qiagen As | Nucleic acid sequencing |
| CA2695264C (en) * | 2007-08-01 | 2015-01-27 | Dana-Farber Cancer Institute | Enrichment of a target sequence |
| US8071338B2 (en) * | 2007-08-08 | 2011-12-06 | Roche Molecular Systems, Inc. | Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity |
| ES2359058B1 (es) * | 2009-07-02 | 2012-03-27 | Consejo Superior De Investigaciones Cient�?Ficas (Csic) | Quimera de adn polimerasa del fago ph1 29. |
| JP5851990B2 (ja) | 2009-07-29 | 2016-02-03 | ネルビアーノ・メデイカル・サイエンシーズ・エツセ・エルレ・エルレ | Ｐｌｋ阻害剤の塩 |
| EP2545189B1 (en) | 2010-03-08 | 2018-01-10 | Dana-Farber Cancer Institute, Inc. | Full cold-pcr enrichment with reference blocking sequence |
-
2012
- 2012-02-28 JP JP2013556801A patent/JP2014507950A/ja active Pending
- 2012-02-28 US US13/407,274 patent/US20120225421A1/en not_active Abandoned
- 2012-02-28 CA CA2828535A patent/CA2828535A1/en not_active Abandoned
- 2012-02-28 CN CN201280020801.3A patent/CN103517993A/zh active Pending
- 2012-02-28 WO PCT/US2012/026938 patent/WO2012118802A1/en not_active Ceased
- 2012-02-28 EP EP12708466.3A patent/EP2681332A1/en not_active Withdrawn
- 2012-02-28 AU AU2012223438A patent/AU2012223438A1/en not_active Abandoned
- 2012-02-28 KR KR1020137025264A patent/KR20140010093A/ko not_active Withdrawn
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