CA2828535A1 - Kit and method for sequencing a target dna in a mixed population - Google Patents

Kit and method for sequencing a target dna in a mixed population Download PDF

Info

Publication number
CA2828535A1
CA2828535A1 CA2828535A CA2828535A CA2828535A1 CA 2828535 A1 CA2828535 A1 CA 2828535A1 CA 2828535 A CA2828535 A CA 2828535A CA 2828535 A CA2828535 A CA 2828535A CA 2828535 A1 CA2828535 A1 CA 2828535A1
Authority
CA
Canada
Prior art keywords
nucleic acid
sequencing
sequence
strand
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2828535A
Other languages
English (en)
French (fr)
Inventor
Katherine RICHARDSON
Benjamin Legendre Jr.
Yanggu Shi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Publication of CA2828535A1 publication Critical patent/CA2828535A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/173—Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2828535A 2011-02-28 2012-02-28 Kit and method for sequencing a target dna in a mixed population Abandoned CA2828535A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161447490P 2011-02-28 2011-02-28
US61/447,490 2011-02-28
US201161532887P 2011-09-09 2011-09-09
US61/532,887 2011-09-09
PCT/US2012/026938 WO2012118802A1 (en) 2011-02-28 2012-02-28 Kit and method for sequencing a target dna in a mixed population

Publications (1)

Publication Number Publication Date
CA2828535A1 true CA2828535A1 (en) 2012-09-07

Family

ID=45815993

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2828535A Abandoned CA2828535A1 (en) 2011-02-28 2012-02-28 Kit and method for sequencing a target dna in a mixed population

Country Status (8)

Country Link
US (1) US20120225421A1 (cg-RX-API-DMAC7.html)
EP (1) EP2681332A1 (cg-RX-API-DMAC7.html)
JP (1) JP2014507950A (cg-RX-API-DMAC7.html)
KR (1) KR20140010093A (cg-RX-API-DMAC7.html)
CN (1) CN103517993A (cg-RX-API-DMAC7.html)
AU (1) AU2012223438A1 (cg-RX-API-DMAC7.html)
CA (1) CA2828535A1 (cg-RX-API-DMAC7.html)
WO (1) WO2012118802A1 (cg-RX-API-DMAC7.html)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2545189B1 (en) 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
WO2014089797A1 (zh) * 2012-12-13 2014-06-19 深圳华大基因科技服务有限公司 用于高通量测序的锁核酸修饰的dna片段
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
KR20160003444A (ko) * 2014-07-01 2016-01-11 주식회사 유진셀 핵산말단분해효소를 이용한 서열-특이적 핵산 검출 방법 및 장치와 이에 사용되는 키트
CN107532203B (zh) * 2015-03-06 2019-09-13 真固生物科技有限公司 重叠扩增子的选择性扩增
JP2018518155A (ja) * 2015-04-15 2018-07-12 ザ ジェネラル ホスピタル コーポレイション Ｌｎａベース突然変異体エンリッチメント次世代シーケンシングアッセイ
WO2016172265A1 (en) * 2015-04-20 2016-10-27 Neogenomics Laboratories, Inc. Method to increase sensitivity of next generation sequencing
WO2017070339A1 (en) * 2015-10-20 2017-04-27 Richardson Katherine Microfluidic device for enrichment of nucleic acid sequence alterations
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
CN115927566B (zh) * 2017-09-20 2025-02-18 深圳华大智造科技股份有限公司 一种用于Small RNA的测序方法、测序试剂和应用
CN114096679B (zh) * 2019-07-11 2024-04-09 学校法人东京理科大学 使用固相载体的核酸扩增方法
CN113567404A (zh) * 2021-06-11 2021-10-29 上海交通大学 一种分析肿瘤细胞耐药性的方法和试剂盒

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
FR2779154B1 (fr) * 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
US6045993A (en) * 1998-05-30 2000-04-04 Visible Genetics Inc. Method, reagent and kit for genotyping of human papillomavirus
DE10012540B4 (de) * 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
JP2004520812A (ja) * 2000-08-30 2004-07-15 ハプロゲン・エルエルシー 対立遺伝子を決定するための方法
GB0406863D0 (en) * 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
CA2695264C (en) * 2007-08-01 2015-01-27 Dana-Farber Cancer Institute Enrichment of a target sequence
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
ES2359058B1 (es) * 2009-07-02 2012-03-27 Consejo Superior De Investigaciones Cient�?Ficas (Csic) Quimera de adn polimerasa del fago ph1 29.
JP5851990B2 (ja) 2009-07-29 2016-02-03 ネルビアーノ・メデイカル・サイエンシーズ・エツセ・エルレ・エルレ Ｐｌｋ阻害剤の塩
EP2545189B1 (en) 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence

Also Published As

Publication number Publication date
JP2014507950A (ja) 2014-04-03
KR20140010093A (ko) 2014-01-23
US20120225421A1 (en) 2012-09-06
WO2012118802A9 (en) 2013-07-18
EP2681332A1 (en) 2014-01-08
WO2012118802A1 (en) 2012-09-07
AU2012223438A1 (en) 2013-09-26
CN103517993A (zh) 2014-01-15

Similar Documents

Publication Publication Date Title
CA2828535A1 (en) Kit and method for sequencing a target dna in a mixed population
JP7209762B2 (ja) 核酸の発現、スプライス変異体、転座、コピー数、またはメチル化変化を識別及び定量化するための方法
EP2183387B1 (en) Analysis of nucleic acids by digital pcr
US9765390B2 (en) Methods, compositions, and kits for rare allele detection
CN105331733B (zh) Egfr基因的多态性检测用探针、扩增用引物及其应用
GB2506760A (en) Primers for detecting SNPs in exon 21 of EGFR
US20160273022A1 (en) Synthesis and enrichment of nucleic acid sequences
CN102242211A (zh) 检测突变核酸序列的方法及试剂盒
WO2020215652A1 (zh) 基于核酸酶偶联pcr原理富集低丰度dna突变的检测技术体系及应用
US20130309724A1 (en) Step-up method for cold-pcr enrichment
WO2010001969A1 (ja) 標的核酸配列の増幅方法、それを用いた変異の検出方法、および、それに用いる試薬
US10351903B2 (en) Allele amplification bias
CN107523630A (zh) 一种基于信号放大dna逻辑门的基因突变多重检测方法
WO2015073163A2 (en) Synthesis and enrichment of nucleic acid sequences
US20060183136A1 (en) Method for haplotyping and genotyping by melting curve analysis of hybridization probes
KR101606530B1 (ko) Hla-b*27 및 hla-b*51의 동시 검출 방법 및 이의 용도
CN102378815B (zh) 等位基因的偏向扩增
JP6754202B2 (ja) Ｋ−ｒａｓ遺伝子増幅用フォワードプライマーセット、Ｋ−ｒａｓ遺伝子増幅用キット、Ｋ−ｒａｓ遺伝子増幅方法、多型解析方法、及び薬効判定方法
CytoScan Genomic Copy Number Findings
EP3837383A1 (en) Reagents, mixtures, kits and methods for amplification of nucleic acids
WO2017070339A1 (en) Microfluidic device for enrichment of nucleic acid sequence alterations
EP2951321A2 (en) Synthesis and enrichment of nucleic acid sequences

Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20180228