JP2014505594A - 液滴を分裂させるためのシステムおよび方法 - Google Patents

液滴を分裂させるためのシステムおよび方法 Download PDF

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JP2014505594A
JP2014505594A JP2013552712A JP2013552712A JP2014505594A JP 2014505594 A JP2014505594 A JP 2014505594A JP 2013552712 A JP2013552712 A JP 2013552712A JP 2013552712 A JP2013552712 A JP 2013552712A JP 2014505594 A JP2014505594 A JP 2014505594A
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droplet
channel
droplets
microfluidic
microfluidic channel
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JP2014505594A5 (enExample
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アダム アール． アバテ，
デイビッド エー． ウェイツ，
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Harvard University
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/8593—Systems
    • Y10T137/85938—Non-valved flow dividers

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
JP2013552712A 2011-02-07 2012-02-06 液滴を分裂させるためのシステムおよび方法 Pending JP2014505594A (ja)

Applications Claiming Priority (3)

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US201161440198P 2011-02-07 2011-02-07
US61/440,198 2011-02-07
PCT/US2012/023961 WO2012109138A1 (en) 2011-02-07 2012-02-06 Systems and methods for splitting droplets

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US (1) US20140026968A1 (enExample)
EP (1) EP2673088A1 (enExample)
JP (2) JP2014505594A (enExample)
KR (1) KR20140063507A (enExample)
CN (2) CN105689030A (enExample)
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Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL229042B1 (pl) * 2011-09-30 2018-06-29 Inst Chemii Fizycznej Polskiej Akademii Nauk Sposób dzielenia kropel w złączu mikroprzepływowym i układ do dzielenia kropel w złączu mikroprzepływowym
US9475013B2 (en) 2012-02-08 2016-10-25 President And Fellows Of Harvard College Droplet formation using fluid breakup
DE102012217487A1 (de) * 2012-09-26 2014-04-17 Agilent Technologies, Inc. - A Delaware Corporation - Fluidschnittstelle zwischen Fluidleitungen unterschiedlicher Querschnitte
EP2964390B1 (en) * 2013-03-06 2018-12-26 President and Fellows of Harvard College Use of devices for forming relatively monodisperse droplets
CN103285946A (zh) * 2013-05-27 2013-09-11 苏州扬清芯片科技有限公司 一种生物芯片及其操控方法
CN103285947A (zh) * 2013-05-27 2013-09-11 苏州扬清芯片科技有限公司 一种液滴微流控芯片及其操控方法
WO2015112985A1 (en) * 2014-01-24 2015-07-30 The Johns Hopkins University System and device for high throughput generation of combinatorial droplets and methods of use
CN105478177A (zh) * 2014-09-18 2016-04-13 苏州贝和医疗科技有限公司 一种用于数字pcr的液滴生成装置及方法
US10178027B2 (en) * 2016-01-27 2019-01-08 Oracle International Corporation System and method for supporting inter subnet partitions in a high performance computing environment
US10171353B2 (en) * 2016-03-04 2019-01-01 Oracle International Corporation System and method for supporting dual-port virtual router in a high performance computing environment
CA3020913C (en) * 2016-04-15 2024-01-02 President And Fellows Of Harvard College Systems and methods for the collection of droplets and/or other entities
US10143129B2 (en) * 2017-02-27 2018-12-04 Cnh Industrial Canada, Ltd. Flow splitter for distributing agricultural products and related system
US10408852B2 (en) 2017-04-26 2019-09-10 Lawrence Livermore National Security, Llc Automated control of microfluidic devices based on machine learning
CN107323090B (zh) * 2017-07-27 2019-01-18 京东方科技集团股份有限公司 一种打印喷头、打印设备及打印方法
CN107754960B (zh) * 2017-10-26 2019-09-03 北京工业大学 基于附加流量实现双乳液滴非对称分裂的微流控芯片
DE102018203047A1 (de) * 2018-03-01 2019-09-05 Robert Bosch Gmbh Mikrofluidische Vorrichtung
CN112399882B (zh) 2018-04-16 2023-08-01 派特恩生物技术有限公司 用于形成二维微滴阵列的方法和设备
CN108956558B (zh) * 2018-05-24 2023-09-15 深圳市帝迈生物技术有限公司 一种微流控芯片和免疫荧光分析仪
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US10486155B1 (en) * 2018-10-22 2019-11-26 Klaris Corporation Vacuum-loaded, droplet-generating microfluidic chips and related methods
CN109289951B (zh) * 2018-10-29 2021-01-05 深圳先进技术研究院 液滴分裂微流控芯片及应用
JP6761153B1 (ja) * 2019-03-20 2020-09-23 京セラ株式会社 粒子計測デバイスならびに粒子分離計測デバイスおよび粒子分離計測装置
CN111821927B (zh) * 2019-04-17 2021-12-07 北京大学 一种制备微/纳结构材料的方法及装置
CN112108192B (zh) * 2019-06-19 2022-03-22 中国科学院大连化学物理研究所 一种微流控芯片及其应用
EP3763438A1 (en) * 2019-07-12 2021-01-13 Curiosity Diagnostics sp. z o.o Microfluidic chip, production process and uses
CN110449195A (zh) * 2019-09-05 2019-11-15 北京工业大学 一种提高液滴在不对称通道分裂均匀度的装置
CN110643488A (zh) * 2019-09-27 2020-01-03 晶准生物医学（深圳）有限公司 微流控液滴操纵分割装置及其操纵分割方法
WO2021168768A1 (zh) * 2020-02-28 2021-09-02 京东方科技集团股份有限公司 一种微流控芯片和微流控系统
CN111537398B (zh) * 2020-05-29 2021-10-15 河南农业大学 一种控制液滴撞击形态、接触时间及液滴可控分裂的方法
CN116171200A (zh) * 2020-09-02 2023-05-26 10X基因组学有限公司 用于高通量液滴形成的装置、系统和方法
EP4180123A1 (en) * 2021-11-12 2023-05-17 Ecole Polytechnique Fédérale de Lausanne (EPFL) EPFL-TTO Microfluidic device for sample encapsulation and method for operation thereof
CN114534811B (zh) * 2022-02-28 2024-10-18 上海天马微电子有限公司 微流控装置及其制作方法
CN115254217B (zh) * 2022-07-27 2023-12-01 领航基因科技（杭州）有限公司 液滴制备装置及方法
CN117205975A (zh) * 2022-10-20 2023-12-12 中国科学技术大学 一种基于约束圆的微流控芯片分叉结构及通用设计方法
CN118892767A (zh) * 2024-07-16 2024-11-05 四川大学 并行多级分裂倍增微流控装置及单分散双重乳液的高通量制备方法
CN119657247B (zh) * 2025-01-20 2025-10-10 中国工程物理研究院激光聚变研究中心 一种基于液滴分裂制备单固核固水油液滴的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010053053A (ja) * 2008-08-27 2010-03-11 Ns Materials Kk ペプチドポリマーの製造方法
JP2010058119A (ja) * 2009-12-07 2010-03-18 Tosoh Corp 微小粒子構造体およびそれを用いた微小粒子の製造方法

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2422804A (en) 1946-01-26 1947-06-24 Walter H Schroeder Kite
US5862808A (en) 1997-08-26 1999-01-26 Cigar Savor Enterprises Llc Cigar punch
WO2001088525A1 (en) * 2000-05-12 2001-11-22 University Of Cincinnati Structurally programmable microfluidic systems
US7901939B2 (en) * 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
JP2006507921A (ja) * 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20060078893A1 (en) * 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
WO2004091763A2 (en) * 2003-04-10 2004-10-28 President And Fellows Of Harvard College Formation and control of fluidic species
WO2004103565A2 (de) * 2003-05-19 2004-12-02 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Vorrichtung und verfahren zur strukturierung von flüssigkeiten und zum zudosieren von reaktionsflüssigkeiten zu in separationsmedium eingebetteten flüssigkeitskompartimenten
EP2662136A3 (en) * 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for handling and mixing droplets
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7655470B2 (en) * 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US7968287B2 (en) * 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1861194A2 (en) 2005-03-04 2007-12-05 The President and Fellows of Harvard College Method and apparatus for forming multiple emulsions
FR2890578B3 (fr) * 2005-09-09 2007-11-30 Rhodia Chimie Sa Dispositif d'ecoulement microfluidique permettant de determiner des parametres d'une transformation physique et/ ou chimique, et son utilisation
CN101300352B (zh) * 2005-09-01 2013-03-20 佳能美国生命科学公司 检测、分析和鉴定基因组dna的方法和分子诊断装置
TWI296711B (en) * 2005-10-11 2008-05-11 Ind Tech Res Inst Biochip with microchannels
ATE416508T1 (de) 2005-10-24 2008-12-15 Conergy Ag Wechselrichter
RU2008127327A (ru) 2005-12-06 2010-01-20 Ибара-Удилайт Ко., Лтд. (Jp) Комплексное соединение палладия и раствор для обеспечения каталитических свойств, содержащий его
US7811603B2 (en) * 2006-05-09 2010-10-12 The Regents Of The University Of California Microfluidic device for forming monodisperse lipoplexes
EP2021113A2 (en) * 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
WO2008063135A1 (en) * 2006-11-24 2008-05-29 Agency For Science, Technology And Research Apparatus for processing a sample in a liquid droplet and method of using the same
US7776927B2 (en) 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
US8883291B2 (en) 2007-08-07 2014-11-11 President And Fellows Of Harvard College Metal oxide coating on surfaces
US8802027B2 (en) 2008-03-28 2014-08-12 President And Fellows Of Harvard College Surfaces, including microfluidic channels, with controlled wetting properties
EP2286125B1 (en) 2008-05-16 2015-07-08 President and Fellows of Harvard College Valves and other flow control in fluidic systems including microfluidic systems
WO2009152363A2 (en) 2008-06-11 2009-12-17 David Stansberry Universal variable transmission line hanger
EP2340435A1 (en) * 2008-10-08 2011-07-06 Université de Strasbourg Microfluidic devices for reliable on-chip incubation of droplets in delay lines
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
US9625454B2 (en) * 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
TWI499552B (zh) * 2012-12-07 2015-09-11 Univ Nat Cheng Kung 液滴產生方法及裝置
US20140161686A1 (en) * 2012-12-10 2014-06-12 Advanced Liquid Logic, Inc. System and method of dispensing liquids in a microfluidic device
US11141730B2 (en) * 2013-06-14 2021-10-12 President And Fellows Of Harvard College Coalescence of droplets
CN105765055A (zh) * 2013-08-27 2016-07-13 基纽拜奥股份有限公司 微流体装置和其使用方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010053053A (ja) * 2008-08-27 2010-03-11 Ns Materials Kk ペプチドポリマーの製造方法
JP2010058119A (ja) * 2009-12-07 2010-03-18 Tosoh Corp 微小粒子構造体およびそれを用いた微小粒子の製造方法

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BR112013019880A2 (pt) 2016-10-11
JP2016224057A (ja) 2016-12-28
KR20140063507A (ko) 2014-05-27
US20140026968A1 (en) 2014-01-30
CN103347612A (zh) 2013-10-09
CN103347612B (zh) 2016-03-16
CN105689030A (zh) 2016-06-22
WO2012109138A1 (en) 2012-08-16
EP2673088A1 (en) 2013-12-18

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