JP2013526273A5 - - Google Patents
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- Publication number
- JP2013526273A5 JP2013526273A5 JP2013510047A JP2013510047A JP2013526273A5 JP 2013526273 A5 JP2013526273 A5 JP 2013526273A5 JP 2013510047 A JP2013510047 A JP 2013510047A JP 2013510047 A JP2013510047 A JP 2013510047A JP 2013526273 A5 JP2013526273 A5 JP 2013526273A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- deoxynucleoside
- fluorescent moiety
- kit
- bound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004414 DNA Proteins 0.000 claims 21
- 102000053602 DNA Human genes 0.000 claims 14
- 238000000034 method Methods 0.000 claims 12
- 239000003298 DNA probe Substances 0.000 claims 8
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 8
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 8
- 230000001419 dependent effect Effects 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 5
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 5
- 239000000523 sample Substances 0.000 claims 5
- 230000000694 effects Effects 0.000 claims 4
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 claims 3
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 claims 3
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 claims 2
- -1 BrdU and / or IdU Chemical class 0.000 claims 2
- 108020001019 DNA Primers Proteins 0.000 claims 2
- 239000003155 DNA primer Substances 0.000 claims 2
- 108030003689 Deoxynucleoside kinases Proteins 0.000 claims 2
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 claims 2
- 108010006785 Taq Polymerase Proteins 0.000 claims 2
- 108010020713 Tth polymerase Proteins 0.000 claims 2
- 102100020797 UMP-CMP kinase Human genes 0.000 claims 2
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical class O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 238000010791 quenching Methods 0.000 claims 2
- 230000000171 quenching effect Effects 0.000 claims 2
- 108010068698 spleen exonuclease Proteins 0.000 claims 2
- 101001138544 Homo sapiens UMP-CMP kinase Proteins 0.000 claims 1
- 108091000080 Phosphotransferase Proteins 0.000 claims 1
- 102100037357 Thymidylate kinase Human genes 0.000 claims 1
- 101710100179 UMP-CMP kinase Proteins 0.000 claims 1
- 101710119674 UMP-CMP kinase 2, mitochondrial Proteins 0.000 claims 1
- 108010000742 dTMP kinase Proteins 0.000 claims 1
- 102000020233 phosphotransferase Human genes 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE1050473-6 | 2010-05-14 | ||
| SE1050473 | 2010-05-14 | ||
| PCT/SE2011/050605 WO2011142719A1 (en) | 2010-05-14 | 2011-05-13 | Kit and method |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016192924A Division JP6409233B2 (ja) | 2010-05-14 | 2016-09-30 | キットおよび方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013526273A JP2013526273A (ja) | 2013-06-24 |
| JP2013526273A5 true JP2013526273A5 (enExample) | 2014-07-03 |
Family
ID=44641364
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013510047A Pending JP2013526273A (ja) | 2010-05-14 | 2011-05-13 | キットおよび方法 |
| JP2016192924A Active JP6409233B2 (ja) | 2010-05-14 | 2016-09-30 | キットおよび方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016192924A Active JP6409233B2 (ja) | 2010-05-14 | 2016-09-30 | キットおよび方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US9429518B2 (enExample) |
| EP (1) | EP2569441B2 (enExample) |
| JP (2) | JP2013526273A (enExample) |
| CN (1) | CN103038361B (enExample) |
| DK (1) | DK2569441T4 (enExample) |
| WO (1) | WO2011142719A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9429518B2 (en) | 2010-05-14 | 2016-08-30 | Csens Ab | Kit and method |
| CN110904187A (zh) * | 2019-11-05 | 2020-03-24 | 翌圣生物科技（上海）有限公司 | 一种Taq酶活性测定方法 |
| JP2025514424A (ja) | 2022-04-29 | 2025-05-02 | ビオヴィカ インターナショナル アクティエ ボラーグ | 免疫チェックポイント阻害剤の有効性のためのマーカーとしてのチミジンキナーゼ |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5273879A (en) * | 1987-07-23 | 1993-12-28 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
| US5328827A (en) * | 1987-08-27 | 1994-07-12 | Institut National De La Recherche Agronomique - Inra | Specific DNA molecular probes for Bos-type male genome |
| DE4020529A1 (de) * | 1989-09-12 | 1991-03-21 | Roxana Vasiloiu | Multifunktionelle(s) enzym(e) mit nucleosid-didesoxyribosyltransferase(n) und/oder nucleoside-desoxyribosyltransferasen(n) und/oder kinease- und/oder reduktase- und/oder desaminase- und/oder polymerase-aktivitaet |
| US5189019A (en) * | 1990-04-23 | 1993-02-23 | Merck & Co., Inc. | Antistasin derived anticoagulant protein |
| US5321010A (en) * | 1991-12-10 | 1994-06-14 | Merck & Co., Inc. | Proteins for inhibiting adhesion of platelets to collagen |
| WO1995012313A1 (en) * | 1993-11-05 | 1995-05-11 | Pathogenesis Corporation | Virus associated multiple sclerosis: treatments, prevention and diagnosis thereof |
| EP0745686A1 (en) * | 1995-06-01 | 1996-12-04 | Roche Diagnostics GmbH | The use of DNA polymerase 3'-intrinsic editing activity |
| GB0022458D0 (en) | 2000-09-13 | 2000-11-01 | Medical Res Council | Directed evolution method |
| US7045319B2 (en) | 2001-10-30 | 2006-05-16 | Ribomed Biotechnologies, Inc. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
| ATE352039T1 (de) | 2002-07-27 | 2007-02-15 | Diasorin Ab | Verfahren zur bestimmung der aktivität von thymidinkinase-1 und dessen verwendung |
| US9505846B2 (en) | 2005-01-28 | 2016-11-29 | Life Technologies Corporation | Multi-component inhibitors of nucleic acid polymerases |
| AU2006217129B2 (en) * | 2005-02-25 | 2011-06-30 | Ronnerbol International Ab | A method and kit for determination of thymidine kinase activity and use thereof |
| WO2008024052A1 (en) | 2006-08-24 | 2008-02-28 | Rönnerbol International Ab | A method and a kit for determination of an enzyme activity involved in metabolic production of a deoxynucleoside triphosphate and use thereof |
| GB0722449D0 (en) | 2007-11-15 | 2007-12-27 | Univ Abertay Dundee | bIOASSAY |
| US20090197254A1 (en) * | 2007-12-14 | 2009-08-06 | Ming-Chou Lee | Variant scorpion primers for nucleic acid amplification and detection |
| JP2009268381A (ja) | 2008-05-01 | 2009-11-19 | Univ Of Fukui | 癌特異的酢酸代謝を標的とした癌バイオマーカー・検査方法・治療剤 |
| GB0814591D0 (en) * | 2008-08-08 | 2008-09-17 | Randox Lab Ltd | Assay |
| WO2010036359A2 (en) * | 2008-09-25 | 2010-04-01 | Allelogic Biosciences Corporation | Methods of polymerase activity assay |
| US9429518B2 (en) | 2010-05-14 | 2016-08-30 | Csens Ab | Kit and method |
-
2011
- 2011-05-13 US US13/697,279 patent/US9429518B2/en active Active
- 2011-05-13 JP JP2013510047A patent/JP2013526273A/ja active Pending
- 2011-05-13 EP EP11780890.7A patent/EP2569441B2/en active Active
- 2011-05-13 DK DK11780890.7T patent/DK2569441T4/en active
- 2011-05-13 CN CN201180024021.1A patent/CN103038361B/zh active Active
- 2011-05-13 WO PCT/SE2011/050605 patent/WO2011142719A1/en not_active Ceased
-
2016
- 2016-08-22 US US15/243,764 patent/US10190149B2/en active Active
- 2016-09-30 JP JP2016192924A patent/JP6409233B2/ja active Active
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