DK2569441T4 - KIT AND METHOD OF MEASURING THE ACTIVITY OF DEOXYNUCLEOSIDE KINASE USING DNA DEPENDENT DNA POLYMERASE AND NATURAL DEOXYNUCLEOSIDES - Google Patents

KIT AND METHOD OF MEASURING THE ACTIVITY OF DEOXYNUCLEOSIDE KINASE USING DNA DEPENDENT DNA POLYMERASE AND NATURAL DEOXYNUCLEOSIDES Download PDF

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Publication number
DK2569441T4
DK2569441T4 DK11780890.7T DK11780890T DK2569441T4 DK 2569441 T4 DK2569441 T4 DK 2569441T4 DK 11780890 T DK11780890 T DK 11780890T DK 2569441 T4 DK2569441 T4 DK 2569441T4
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Denmark
Prior art keywords
dna
kit
kinase
activity
probe
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DK11780890.7T
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Danish (da)
English (en)
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DK2569441T3 (en
Inventor
Johan Lennerstrand
Per Stålhandske
Original Assignee
Csens Ab
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Publication of DK2569441T3 publication Critical patent/DK2569441T3/en
Publication of DK2569441T4 publication Critical patent/DK2569441T4/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • C12Y207/07007DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/91Transferases (2.)
    • G01N2333/912Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205Phosphotransferases in general
    • G01N2333/91235Phosphotransferases in general with a phosphate group as acceptor (2.7.4)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/91Transferases (2.)
    • G01N2333/912Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205Phosphotransferases in general
    • G01N2333/91245Nucleotidyltransferases (2.7.7)
    • G01N2333/9125Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
    • G01N2333/9126DNA-directed DNA polymerase (2.7.7.7)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DK11780890.7T 2010-05-14 2011-05-13 KIT AND METHOD OF MEASURING THE ACTIVITY OF DEOXYNUCLEOSIDE KINASE USING DNA DEPENDENT DNA POLYMERASE AND NATURAL DEOXYNUCLEOSIDES DK2569441T4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE1050473 2010-05-14
PCT/SE2011/050605 WO2011142719A1 (en) 2010-05-14 2011-05-13 Kit and method

Publications (2)

Publication Number Publication Date
DK2569441T3 DK2569441T3 (en) 2015-01-05
DK2569441T4 true DK2569441T4 (en) 2017-12-18

Family

ID=44641364

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11780890.7T DK2569441T4 (en) 2010-05-14 2011-05-13 KIT AND METHOD OF MEASURING THE ACTIVITY OF DEOXYNUCLEOSIDE KINASE USING DNA DEPENDENT DNA POLYMERASE AND NATURAL DEOXYNUCLEOSIDES

Country Status (6)

Country Link
US (2) US9429518B2 (enExample)
EP (1) EP2569441B2 (enExample)
JP (2) JP2013526273A (enExample)
CN (1) CN103038361B (enExample)
DK (1) DK2569441T4 (enExample)
WO (1) WO2011142719A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9429518B2 (en) 2010-05-14 2016-08-30 Csens Ab Kit and method
CN110904187A (zh) * 2019-11-05 2020-03-24 翌圣生物科技(上海)有限公司 一种Taq酶活性测定方法
JP2025514424A (ja) 2022-04-29 2025-05-02 ビオヴィカ インターナショナル アクティエ ボラーグ 免疫チェックポイント阻害剤の有効性のためのマーカーとしてのチミジンキナーゼ

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5328827A (en) * 1987-08-27 1994-07-12 Institut National De La Recherche Agronomique - Inra Specific DNA molecular probes for Bos-type male genome
DE4020529A1 (de) * 1989-09-12 1991-03-21 Roxana Vasiloiu Multifunktionelle(s) enzym(e) mit nucleosid-didesoxyribosyltransferase(n) und/oder nucleoside-desoxyribosyltransferasen(n) und/oder kinease- und/oder reduktase- und/oder desaminase- und/oder polymerase-aktivitaet
US5189019A (en) * 1990-04-23 1993-02-23 Merck & Co., Inc. Antistasin derived anticoagulant protein
US5321010A (en) * 1991-12-10 1994-06-14 Merck & Co., Inc. Proteins for inhibiting adhesion of platelets to collagen
WO1995012313A1 (en) * 1993-11-05 1995-05-11 Pathogenesis Corporation Virus associated multiple sclerosis: treatments, prevention and diagnosis thereof
EP0745686A1 (en) * 1995-06-01 1996-12-04 Roche Diagnostics GmbH The use of DNA polymerase 3'-intrinsic editing activity
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US7045319B2 (en) 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
ATE352039T1 (de) 2002-07-27 2007-02-15 Diasorin Ab Verfahren zur bestimmung der aktivität von thymidinkinase-1 und dessen verwendung
US9505846B2 (en) 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
AU2006217129B2 (en) * 2005-02-25 2011-06-30 Ronnerbol International Ab A method and kit for determination of thymidine kinase activity and use thereof
WO2008024052A1 (en) 2006-08-24 2008-02-28 Rönnerbol International Ab A method and a kit for determination of an enzyme activity involved in metabolic production of a deoxynucleoside triphosphate and use thereof
GB0722449D0 (en) 2007-11-15 2007-12-27 Univ Abertay Dundee bIOASSAY
US20090197254A1 (en) * 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
JP2009268381A (ja) 2008-05-01 2009-11-19 Univ Of Fukui 癌特異的酢酸代謝を標的とした癌バイオマーカー・検査方法・治療剤
GB0814591D0 (en) * 2008-08-08 2008-09-17 Randox Lab Ltd Assay
WO2010036359A2 (en) * 2008-09-25 2010-04-01 Allelogic Biosciences Corporation Methods of polymerase activity assay
US9429518B2 (en) 2010-05-14 2016-08-30 Csens Ab Kit and method

Also Published As

Publication number Publication date
JP2017042169A (ja) 2017-03-02
EP2569441A1 (en) 2013-03-20
WO2011142719A1 (en) 2011-11-17
DK2569441T3 (en) 2015-01-05
JP2013526273A (ja) 2013-06-24
JP6409233B2 (ja) 2018-10-24
US20130071846A1 (en) 2013-03-21
US9429518B2 (en) 2016-08-30
EP2569441B1 (en) 2014-11-19
EP2569441A4 (en) 2013-11-27
CN103038361A (zh) 2013-04-10
US10190149B2 (en) 2019-01-29
EP2569441B2 (en) 2017-09-13
CN103038361B (zh) 2017-02-08
US20170029867A1 (en) 2017-02-02

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