JP2013526273A - キットおよび方法 - Google Patents

キットおよび方法 Download PDF

Info

Publication number
JP2013526273A
JP2013526273A JP2013510047A JP2013510047A JP2013526273A JP 2013526273 A JP2013526273 A JP 2013526273A JP 2013510047 A JP2013510047 A JP 2013510047A JP 2013510047 A JP2013510047 A JP 2013510047A JP 2013526273 A JP2013526273 A JP 2013526273A
Authority
JP
Japan
Prior art keywords
dna
polymerase
fluorescent moiety
deoxynucleoside
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2013510047A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013526273A5 (enExample
Inventor
ストールハンシェ，ペール
レネルストランド，ユハン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stalhandske per
Original Assignee
Stalhandske per
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44641364&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP2013526273(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Stalhandske per filed Critical Stalhandske per
Publication of JP2013526273A publication Critical patent/JP2013526273A/ja
Publication of JP2013526273A5 publication Critical patent/JP2013526273A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91235—Phosphotransferases in general with a phosphate group as acceptor (2.7.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
    • G01N2333/9126—DNA-directed DNA polymerase (2.7.7.7)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2013510047A 2010-05-14 2011-05-13 キットおよび方法 Pending JP2013526273A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE1050473-6 2010-05-14
SE1050473 2010-05-14
PCT/SE2011/050605 WO2011142719A1 (en) 2010-05-14 2011-05-13 Kit and method

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016192924A Division JP6409233B2 (ja) 2010-05-14 2016-09-30 キットおよび方法

Publications (2)

Publication Number Publication Date
JP2013526273A true JP2013526273A (ja) 2013-06-24
JP2013526273A5 JP2013526273A5 (enExample) 2014-07-03

Family

ID=44641364

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2013510047A Pending JP2013526273A (ja) 2010-05-14 2011-05-13 キットおよび方法
JP2016192924A Active JP6409233B2 (ja) 2010-05-14 2016-09-30 キットおよび方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2016192924A Active JP6409233B2 (ja) 2010-05-14 2016-09-30 キットおよび方法

Country Status (6)

Country Link
US (2) US9429518B2 (enExample)
EP (1) EP2569441B2 (enExample)
JP (2) JP2013526273A (enExample)
CN (1) CN103038361B (enExample)
DK (1) DK2569441T4 (enExample)
WO (1) WO2011142719A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9429518B2 (en) 2010-05-14 2016-08-30 Csens Ab Kit and method
CN110904187A (zh) * 2019-11-05 2020-03-24 翌圣生物科技（上海）有限公司 一种Taq酶活性测定方法
JP2025514424A (ja) 2022-04-29 2025-05-02 ビオヴィカ インターナショナル アクティエ ボラーグ 免疫チェックポイント阻害剤の有効性のためのマーカーとしてのチミジンキナーゼ

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008531012A (ja) * 2005-02-25 2008-08-14 レンネルボル・ホールディング・アクチボラゲット チミジンキナーゼ活性の測定のための方法およびキットならびにその使用
JP2009268381A (ja) * 2008-05-01 2009-11-19 Univ Of Fukui 癌特異的酢酸代謝を標的とした癌バイオマーカー・検査方法・治療剤

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5328827A (en) * 1987-08-27 1994-07-12 Institut National De La Recherche Agronomique - Inra Specific DNA molecular probes for Bos-type male genome
DE4020529A1 (de) * 1989-09-12 1991-03-21 Roxana Vasiloiu Multifunktionelle(s) enzym(e) mit nucleosid-didesoxyribosyltransferase(n) und/oder nucleoside-desoxyribosyltransferasen(n) und/oder kinease- und/oder reduktase- und/oder desaminase- und/oder polymerase-aktivitaet
US5189019A (en) * 1990-04-23 1993-02-23 Merck & Co., Inc. Antistasin derived anticoagulant protein
US5321010A (en) * 1991-12-10 1994-06-14 Merck & Co., Inc. Proteins for inhibiting adhesion of platelets to collagen
WO1995012313A1 (en) * 1993-11-05 1995-05-11 Pathogenesis Corporation Virus associated multiple sclerosis: treatments, prevention and diagnosis thereof
EP0745686A1 (en) * 1995-06-01 1996-12-04 Roche Diagnostics GmbH The use of DNA polymerase 3'-intrinsic editing activity
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US7045319B2 (en) 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
ATE352039T1 (de) 2002-07-27 2007-02-15 Diasorin Ab Verfahren zur bestimmung der aktivität von thymidinkinase-1 und dessen verwendung
US9505846B2 (en) 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
WO2008024052A1 (en) 2006-08-24 2008-02-28 Rönnerbol International Ab A method and a kit for determination of an enzyme activity involved in metabolic production of a deoxynucleoside triphosphate and use thereof
GB0722449D0 (en) 2007-11-15 2007-12-27 Univ Abertay Dundee bIOASSAY
US20090197254A1 (en) * 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
GB0814591D0 (en) * 2008-08-08 2008-09-17 Randox Lab Ltd Assay
WO2010036359A2 (en) * 2008-09-25 2010-04-01 Allelogic Biosciences Corporation Methods of polymerase activity assay
US9429518B2 (en) 2010-05-14 2016-08-30 Csens Ab Kit and method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008531012A (ja) * 2005-02-25 2008-08-14 レンネルボル・ホールディング・アクチボラゲット チミジンキナーゼ活性の測定のための方法およびキットならびにその使用
JP2009268381A (ja) * 2008-05-01 2009-11-19 Univ Of Fukui 癌特異的酢酸代謝を標的とした癌バイオマーカー・検査方法・治療剤

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6015026433; Ａｎａｌｙ．　Ｂｉｏｃｈｅｍ． Ｖｏｌ．３６９, 2000, ｐ８０-８６ *
JPN6015026434; Ｂｉｏｃｈｅｍ．　Ｐｈａｒｍａｃｏｌｏｇｙ Ｖｏｌ．７１, 2006, ｐ８８２-８９０ *

Also Published As

Publication number Publication date
DK2569441T4 (en) 2017-12-18
JP2017042169A (ja) 2017-03-02
EP2569441A1 (en) 2013-03-20
WO2011142719A1 (en) 2011-11-17
DK2569441T3 (en) 2015-01-05
JP6409233B2 (ja) 2018-10-24
US20130071846A1 (en) 2013-03-21
US9429518B2 (en) 2016-08-30
EP2569441B1 (en) 2014-11-19
EP2569441A4 (en) 2013-11-27
CN103038361A (zh) 2013-04-10
US10190149B2 (en) 2019-01-29
EP2569441B2 (en) 2017-09-13
CN103038361B (zh) 2017-02-08
US20170029867A1 (en) 2017-02-02

Similar Documents

Publication Publication Date Title
US11208688B2 (en) Small RNA capture, detection and quantification
JP4558932B2 (ja) ハイブリダイゼーション及び不一致識別のための、ピラゾロ［３，４−ｄ］ピリミジン含有オリゴヌクレオチド
CN114250278B (zh) 捕获、检测和定量小rna的方法、组合物与试剂盒
RU2630999C2 (ru) Композиции для реакции обратной транскрипции с горячим стартом или для полимеразной цепной реакции с обратной транскрипцией с горячим стартом
US9315860B2 (en) Conjugates of nucleotides and method for the application thereof
JP2004532615A (ja) 核酸の検出および識別のためのプライマーおよび方法
KR20230012467A (ko) 표적 핵산을 검출하기 위한 루프형 프라이머 및 루프-드-루프 방법
US10087482B2 (en) Amplification of bisulfite-reacted nucleic acids
JP6409233B2 (ja) キットおよび方法
US20110212451A1 (en) Rna detection method
CA3223987A1 (en) Methods, compositions, and kits for preparing sequencing library
WO2025210056A1 (en) In vitro amplification of dna methylation patterns
CN101671672B (zh) 用于改进的核酸扩增的聚阴离子
JP7719721B2 (ja) 核酸を検出するための係留酵素の使用
JP2023552693A (ja) 標的遺伝子変異及びウイルスゲノムの検出システム並びにその製造及び使用の方法
EP4274911A1 (en) Sequencing an insert and an identifier without denaturation

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140513

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140513

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20150220

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20150220

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150630

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150929

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151027

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151130

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160104

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20160531