JP2013505739A - 乳酸菌におけるアセト乳酸誘導生成物への改善されたフラックス - Google Patents

乳酸菌におけるアセト乳酸誘導生成物への改善されたフラックス Download PDF

Info

Publication number
JP2013505739A
JP2013505739A JP2012532272A JP2012532272A JP2013505739A JP 2013505739 A JP2013505739 A JP 2013505739A JP 2012532272 A JP2012532272 A JP 2012532272A JP 2012532272 A JP2012532272 A JP 2012532272A JP 2013505739 A JP2013505739 A JP 2013505739A
Authority
JP
Japan
Prior art keywords
seq
lactic acid
cells
lactate dehydrogenase
acid bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2012532272A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013505739A5 (https=
Inventor
ブライアン・ジェームズ・ポール
ウォンチョル・シュウ
Original Assignee
ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー filed Critical ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー
Publication of JP2013505739A publication Critical patent/JP2013505739A/ja
Publication of JP2013505739A5 publication Critical patent/JP2013505739A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01005—Acetolactate decarboxylase (4.1.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2012532272A 2009-09-29 2010-09-29 乳酸菌におけるアセト乳酸誘導生成物への改善されたフラックス Pending JP2013505739A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24671709P 2009-09-29 2009-09-29
US61/246,717 2009-09-29
PCT/US2010/050705 WO2011041402A1 (en) 2009-09-29 2010-09-29 Improved flux to acetolactate-derived products in lactic acid bacteria

Publications (2)

Publication Number Publication Date
JP2013505739A true JP2013505739A (ja) 2013-02-21
JP2013505739A5 JP2013505739A5 (https=) 2013-11-14

Family

ID=43093547

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012532272A Pending JP2013505739A (ja) 2009-09-29 2010-09-29 乳酸菌におけるアセト乳酸誘導生成物への改善されたフラックス

Country Status (9)

Country Link
US (1) US9169499B2 (https=)
EP (1) EP2483400A1 (https=)
JP (1) JP2013505739A (https=)
CN (1) CN102712912A (https=)
AU (1) AU2010300722A1 (https=)
BR (1) BR112012006941A2 (https=)
CA (1) CA2776151A1 (https=)
IN (1) IN2012DN02396A (https=)
WO (1) WO2011041402A1 (https=)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
CN103540559A (zh) 2007-02-09 2014-01-29 加利福尼亚大学董事会 利用重组微生物生产生物燃料
CN101680007A (zh) 2007-04-18 2010-03-24 纳幕尔杜邦公司 使用高活性酮醇酸还原异构酶来发酵生产异丁醇
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
NZ591126A (en) 2008-09-29 2012-08-31 Butamax Tm Advanced Biofuels INCREASED HETEROLOGOUS Fe-S ENZYME ACTIIVTY IN YEAST
BR112012006939A2 (pt) * 2009-09-29 2015-09-08 Butamax Tm Advanced Biofuels célula hospedeira de produção de levedura recombinante e método para a produção de um produto selecionado do grupo que consiste em 2,3-butanodiol, isobutanol, 2-butanol, 1-butanol, 2-butanona, valina, leucina, ácido lático, ácido málico, álcool, isoampilico e isoprenoides
JP2013515507A (ja) 2009-12-29 2013-05-09 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー 組換え宿主細胞におけるヘキソースキナーゼの発現
NZ600509A (en) 2009-12-29 2014-08-29 Butamax Tm Advanced Biofuels Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
BR112012020589B8 (pt) 2010-02-17 2021-05-18 Butamax Tm Advanced Biofuels célula hospedeira recombinante de levedura, método para fabricar um produto, método para converter 2,3-diidroxiisovalerato em alfa-cetoisovalerato, método para aumentar a atividade específica de um polipeptídeo heterólogo, método para aumentar o fluxo em uma via biossintética e método para aumentar a atividade de uma proteína
CA2810244C (en) 2010-09-07 2020-10-06 Butamax(Tm) Advanced Biofuels Llc Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
WO2012129555A2 (en) 2011-03-24 2012-09-27 Butamax (Tm) Advanced Biofuels Llc Host cells and methods for production of isobutanol
SG194691A1 (en) 2011-04-29 2013-12-30 Danisco Us Inc Production of mevalonate, isoprene, and isoprenoids using genes encodingpolypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities
AU2012286640B2 (en) 2011-07-28 2016-09-01 Butamax Advanced Biofuels Llc Keto-isovalerate decarboxylase enzymes and methods of use thereof
CA2861613A1 (en) 2011-12-30 2013-07-04 Butamax Advanced Biofuels Llc Fermentative production of alcohols
CN102559736B (zh) * 2012-02-24 2014-01-08 常茂生物化学工程股份有限公司 提高富马酸产量的基因工程菌株及构建方法及其应用
CA2868153A1 (en) 2012-03-23 2013-09-26 Butamax Advanced Biofuels Llc Acetate supplemention of medium for butanologens
CN104619835B (zh) 2012-05-11 2018-06-19 布特马斯先进生物燃料有限责任公司 酮醇酸还原异构酶及其使用方法
CN102816787B (zh) * 2012-06-20 2015-05-06 江南大学 利用重组大肠杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶
WO2014025604A2 (en) * 2012-08-07 2014-02-13 Gevo, Inc. Microorganisms for improved production of fuels, chemicals, and amino acids
AU2013305714A1 (en) 2012-08-22 2015-02-05 Butamax Advanced Biofuels Llc Production of fermentation products
CN102876704A (zh) * 2012-09-20 2013-01-16 江南大学 利用重组枯草芽孢杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶
WO2014047421A1 (en) 2012-09-21 2014-03-27 Butamax(Tm) Advanced Biofuels Llc Production of renewable hydrocarbon compositions
CA2886157A1 (en) 2012-09-26 2014-04-03 Butamax Advanced Biofuels Llc Polypeptides with ketol-acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
US9650624B2 (en) 2012-12-28 2017-05-16 Butamax Advanced Biofuels Llc DHAD variants for butanol production
AU2014244216A1 (en) 2013-03-14 2015-09-24 E.I. Du Pont De Nemours And Company Glycerol 3- phosphate dehydrogenase for butanol production
US9771602B2 (en) 2013-03-15 2017-09-26 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
WO2014151190A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Dhad variants and methods of screening
US20150122558A1 (en) 2013-09-09 2015-05-07 Columbia Trailer Co., Inc. Lifting jack assembly with rotatable hydraulic cylinder for steering and method of operation
JP6499587B2 (ja) * 2013-11-22 2019-04-10 Ｊｍｔｃエンザイム株式会社 形質転換体およびその製造方法、ならびに乳酸の製造方法
CA2957920A1 (en) 2014-08-11 2016-02-18 Butamax Advanced Biofuels Llc Yeast preparations and methods of making the same
US10155967B2 (en) * 2015-06-12 2018-12-18 Cj Cheiljedang Corporation Microorganism having enhanced productivity of lactic acid and a process for producing lactic acid using the same
US10563271B2 (en) * 2015-10-09 2020-02-18 Danmarks Tekniske Universitet High-level production of diacetyl in a metabolically engineered lactic acid bacterium
JP7182545B2 (ja) * 2016-12-14 2022-12-02 ヴァーヘニンゲン ユニヴェルシテット 熱安定性ｃａｓ９ヌクレアーゼ
CN108570438B (zh) * 2017-03-08 2020-06-30 中国科学院上海高等研究院 提升克雷伯氏肺炎杆菌生产α-酮异戊酸的方法及改造菌
CN108486176B (zh) * 2018-05-18 2021-08-03 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
IT201800007846A1 (it) * 2018-08-03 2020-02-03 Università Degli Studi Di Milano - Bicocca Procedimento per la biosintesi cellulare di acido poli d-lattico e di acido poli l-lattico
BR112021014779A2 (pt) * 2019-02-01 2021-10-05 Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement Cepas de lactococcus lactis para a prevenção e/ou o tratamento de dor visceral
CN111893083A (zh) * 2019-05-05 2020-11-06 中国科学院上海高等研究院 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用
CN113278655B (zh) * 2020-05-13 2022-05-17 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
BR9711341A (pt) * 1996-08-22 1999-08-17 Hansens Lab BactïÕïrias do cido l ctico metabolicamente manipuladas e seu uso
FR2777905B1 (fr) * 1998-04-23 2002-07-19 Agronomique Inst Nat Rech Bacteries surproductrices d'alpha-acetolactate, et procede d'obtention
DE19855312A1 (de) 1998-12-01 2000-06-08 Degussa Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien
US20080026123A1 (en) 2003-05-22 2008-01-31 Hilltop Ranch, Inc. Apparatus and Process for Almond Pasteurization
TWI348262B (en) 2005-02-10 2011-09-01 Bruno Ferrario A circuit and method for adaptive frequency compensation for dc-to-dc converter
EP1869194A2 (en) 2005-04-12 2007-12-26 E.I.Du pont de nemours and company Treatment of biomass to obtain a target chemical
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
RU2394913C2 (ru) 2005-10-26 2010-07-20 Е.И.Дюпон Де Немур Энд Компани Ферментативное получение четырехуглеродных спиртов
CN101680007A (zh) 2007-04-18 2010-03-24 纳幕尔杜邦公司 使用高活性酮醇酸还原异构酶来发酵生产异丁醇
US8130756B2 (en) 2007-07-13 2012-03-06 Hewlett-Packard Development Company, L.P. Tunnel configuration associated with packet checking in a network
WO2009085953A2 (en) 2007-12-20 2009-07-09 E. I. Du Pont De Nemours And Company Ketol-acid reductoisomerase using nadh
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
US8828695B2 (en) 2008-06-04 2014-09-09 Butamax Advanced Biofuels Llc Method for producing butanol using two-phase extractive fermentation
BRPI0909989A2 (pt) 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc célula de levedura recombinante e método para a produção de um produto
WO2010037105A1 (en) 2008-09-29 2010-04-01 Butamax™ Advanced Biofuels LLC Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
BRPI0913680A2 (pt) 2008-09-29 2019-09-24 Butamax Advanced Biofuels Llc "célula bacteriana ácido-láctica, método para aumentar a atividade de um polipeptídeo dihidroxi-ácido desidratase heterólogo em uma célula bacteriana ácido-láctica e método de produção de insobutanol"
US8455224B2 (en) 2008-09-29 2013-06-04 Butamax(Tm) Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
BRPI0914521A2 (pt) 2008-10-27 2016-07-26 Butamax Advanced Biofuels Llc célula hospedeira microbiana recombinante, método de aumento da produção de isobutanol e método de produção de isobutanol
US20110195505A1 (en) 2009-10-08 2011-08-11 Butamax(Tm) Advanced Biofuels Llc Bacterial strains for butanol production
PL2957322T3 (pl) 2014-06-18 2020-05-18 Fotona D.O.O. Laserowa głowica zabiegowa i system laserowy
US10078608B2 (en) 2016-09-16 2018-09-18 Intel Corporation USB-C multiple connector support for host and device mode configurations

Also Published As

Publication number Publication date
EP2483400A1 (en) 2012-08-08
WO2011041402A1 (en) 2011-04-07
BR112012006941A2 (pt) 2015-09-08
CA2776151A1 (en) 2011-04-07
AU2010300722A1 (en) 2012-04-12
US9169499B2 (en) 2015-10-27
US20110136192A1 (en) 2011-06-09
CN102712912A (zh) 2012-10-03
IN2012DN02396A (https=) 2015-08-21

Similar Documents

Publication Publication Date Title
US9169499B2 (en) Flux to acetolactate-derived products in lactic acid bacteria
JP5805094B2 (ja) 高有効性のケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生産
US9080179B2 (en) Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
WO2010037105A9 (en) Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
US20110195505A1 (en) Bacterial strains for butanol production
US20140186928A1 (en) Recombinant Microorganism and Methods of Production Thereof
JP2009539407A (ja) 溶媒耐性微生物および単離方法
JP2011522541A (ja) イソブタノールを生産するための欠失突然変異体
US20150376654A1 (en) Recombinant microorganisms with increased tolerance to ethanol
KR102109763B1 (ko) 2,3―부탄디올의 생성능이 증강된 재조합 미생물 및 이를 이용한 2,3―부탄디올의 생산 방법
JP6454000B2 (ja) 一酸化炭素デヒドロゲナーゼ（ｃｏｄｈ）活性が改変された遺伝子組換え微生物
CN114901827A (zh) 在乙酰乳酸脱羧酶基因座敲入的微生物
EP0560885A1 (en) Recombinant cells that highly express chromosomally-integrated heterologous genes
EP4689059A1 (fr) Bacteries clostridium modifiees, outil d'edition genetique du plasmide psol de bacteries clostridium, et utilisations
JP2006230329A (ja) 酢酸発酵能が増強された酢酸菌、及び該酢酸菌を用いた食酢の製造方法
CN121100174A (zh) 能够生产丙-2-醇的修饰的梭菌属细菌、其制备和用途
JP2006246701A (ja) 中央代謝系の酵素活性が増強された酢酸菌、及び該酢酸菌を用いた食酢の製造方法
JP2005013105A (ja) 酢酸耐性に関与する遺伝子、該遺伝子を用いて育種された酢酸菌、及び該酢酸菌を用いた食酢の製造方法
NZ614459A (en) Recombinant microorganisms and uses therefor

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130927

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130927

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150106

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150406

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20150630