JP2013059275A5 - - Google Patents
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- Publication number
- JP2013059275A5 JP2013059275A5 JP2011198949A JP2011198949A JP2013059275A5 JP 2013059275 A5 JP2013059275 A5 JP 2013059275A5 JP 2011198949 A JP2011198949 A JP 2011198949A JP 2011198949 A JP2011198949 A JP 2011198949A JP 2013059275 A5 JP2013059275 A5 JP 2013059275A5
- Authority
- JP
- Japan
- Prior art keywords
- exchange resin
- nucleic acid
- cation exchange
- acid purification
- purification method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 19
- 238000001821 nucleic acid purification Methods 0.000 claims description 16
- 239000003957 anion exchange resin Substances 0.000 claims description 13
- 150000002500 ions Chemical class 0.000 claims description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims 20
- 239000003729 cation exchange resin Substances 0.000 claims 17
- 102000039446 nucleic acids Human genes 0.000 claims 10
- 108020004707 nucleic acids Proteins 0.000 claims 10
- 150000007523 nucleic acids Chemical class 0.000 claims 10
- 239000000126 substance Substances 0.000 claims 8
- 238000001962 electrophoresis Methods 0.000 claims 3
- 238000000605 extraction Methods 0.000 claims 3
- 239000003456 ion exchange resin Substances 0.000 claims 3
- 229920003303 ion-exchange polymer Polymers 0.000 claims 3
- 150000001875 compounds Chemical class 0.000 claims 2
- 238000006482 condensation reaction Methods 0.000 claims 2
- 230000018044 dehydration Effects 0.000 claims 2
- 238000006297 dehydration reaction Methods 0.000 claims 2
- 125000000524 functional group Chemical group 0.000 claims 2
- 230000002378 acidificating effect Effects 0.000 claims 1
- 125000000129 anionic group Chemical group 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- 230000007717 exclusion Effects 0.000 claims 1
- 229920001477 hydrophilic polymer Polymers 0.000 claims 1
- 239000000138 intercalating agent Substances 0.000 claims 1
- 238000005342 ion exchange Methods 0.000 claims 1
- 239000002184 metal Substances 0.000 claims 1
- 239000002736 nonionic surfactant Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000012535 impurity Substances 0.000 description 2
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011198949A JP5899731B2 (ja) | 2011-09-13 | 2011-09-13 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
| PCT/JP2012/005384 WO2013038604A1 (ja) | 2011-09-13 | 2012-08-28 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
| US14/239,340 US9498737B2 (en) | 2011-09-13 | 2012-08-28 | Method of purifying nucleic acids, method of extracting nucleic acids and kit for purifying nucleic acids |
| EP12832581.8A EP2757156B1 (en) | 2011-09-13 | 2012-08-28 | Nucleic acid purification method, nucleic acid extraction method, and nucleic acid purification kit |
| CN201280043107.3A CN103781907B (zh) | 2011-09-13 | 2012-08-28 | 纯化核酸的方法、提取核酸的方法和用于纯化核酸的试剂盒 |
| US15/295,720 US10023860B2 (en) | 2011-09-13 | 2016-10-17 | Method of purifying nucleic acids and kit for purifying nucleic acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011198949A JP5899731B2 (ja) | 2011-09-13 | 2011-09-13 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013059275A JP2013059275A (ja) | 2013-04-04 |
| JP2013059275A5 true JP2013059275A5 (enExample) | 2014-10-16 |
| JP5899731B2 JP5899731B2 (ja) | 2016-04-06 |
Family
ID=47882857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011198949A Expired - Fee Related JP5899731B2 (ja) | 2011-09-13 | 2011-09-13 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9498737B2 (enExample) |
| EP (1) | EP2757156B1 (enExample) |
| JP (1) | JP5899731B2 (enExample) |
| CN (1) | CN103781907B (enExample) |
| WO (1) | WO2013038604A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5899731B2 (ja) * | 2011-09-13 | 2016-04-06 | ソニー株式会社 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
| WO2016183012A1 (en) * | 2015-05-11 | 2016-11-17 | 3M Innovative Properties Company | Composition for reducing inhibition of nucleic acid amplification |
| FR3038616B1 (fr) * | 2015-07-06 | 2020-11-06 | Gl Biocontrol | Procede de purification et de concentration d'acides nucleiques. |
| WO2020047095A1 (en) * | 2018-08-28 | 2020-03-05 | The Scripps Research Institute | Use of non-covalent immobilization in dna encoded libraries |
| JPWO2023095704A1 (enExample) * | 2021-11-25 | 2023-06-01 | ||
| KR102654881B1 (ko) * | 2023-06-23 | 2024-04-17 | 주식회사 진시스템 | 핵산 분리 장치 및 방법 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| US6270970B1 (en) * | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
| US6310199B1 (en) * | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| US6504021B2 (en) * | 2000-07-05 | 2003-01-07 | Edge Biosystems, Inc. | Ion exchange method for DNA purification |
| US20030065285A1 (en) * | 2001-07-23 | 2003-04-03 | Higuchi William I. | Method and apparatus for increasing flux during reverse iontophoresis |
| US20050032105A1 (en) * | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
| GB0212826D0 (en) * | 2002-05-31 | 2002-07-10 | Dna Res Innovations Ltd | Materials and methods relating to polyions and substance delivery |
| US20040018559A1 (en) * | 2002-07-26 | 2004-01-29 | Applera Corporation | Size-exclusion ion-exchange particles |
| GB0229287D0 (en) * | 2002-12-16 | 2003-01-22 | Dna Res Innovations Ltd | Polyfunctional reagents |
| US7964343B2 (en) * | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| JP2005080555A (ja) | 2003-09-08 | 2005-03-31 | Arkray Inc | 核酸の濃縮方法 |
| KR100647306B1 (ko) * | 2004-12-23 | 2006-11-23 | 삼성전자주식회사 | 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 |
| KR100657957B1 (ko) * | 2005-04-12 | 2006-12-14 | 삼성전자주식회사 | 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 및 상기방법에 사용될 수 있는 핵산 분리용 고상 물질 |
| WO2008035991A2 (en) * | 2006-09-19 | 2008-03-27 | Michael Ronald Cook | A nucleic acid extraction method |
| GB2445441B (en) * | 2006-09-26 | 2010-06-30 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
| WO2008042218A1 (en) * | 2006-10-03 | 2008-04-10 | Tris Pharma, Inc. | Formulations containing an ionic mineral-ion exchange resin complex and uses thereof |
| US9347055B2 (en) | 2007-11-05 | 2016-05-24 | Eiken Kagaku Kabushiki Kaisha | Method and kit for preparation of sample for use in nucleic acid amplification |
| AU2009242587B2 (en) * | 2008-04-30 | 2015-05-28 | Strike Bio, Inc. | Highly pure plasmid DNA preparations and processes for preparing the same |
| FR2933989B1 (fr) * | 2008-07-16 | 2013-03-08 | Commissariat Energie Atomique | Procede de purification de microorganismes presents dans des echantillons liquides |
| DE102008063001A1 (de) | 2008-12-23 | 2010-06-24 | Qiagen Gmbh | Nukleinsäureaufreinigungsverfahren |
| US8415467B2 (en) * | 2009-12-14 | 2013-04-09 | Betty Wu | Method and materials for separating nucleic acid materials |
| JP5899731B2 (ja) * | 2011-09-13 | 2016-04-06 | ソニー株式会社 | 核酸精製方法、核酸抽出方法、及び核酸精製用キット |
-
2011
- 2011-09-13 JP JP2011198949A patent/JP5899731B2/ja not_active Expired - Fee Related
-
2012
- 2012-08-28 EP EP12832581.8A patent/EP2757156B1/en not_active Not-in-force
- 2012-08-28 CN CN201280043107.3A patent/CN103781907B/zh not_active Expired - Fee Related
- 2012-08-28 US US14/239,340 patent/US9498737B2/en not_active Expired - Fee Related
- 2012-08-28 WO PCT/JP2012/005384 patent/WO2013038604A1/ja not_active Ceased
-
2016
- 2016-10-17 US US15/295,720 patent/US10023860B2/en active Active
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