JP2012528567A5 - - Google Patents
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- JP2012528567A5 JP2012528567A5 JP2012504107A JP2012504107A JP2012528567A5 JP 2012528567 A5 JP2012528567 A5 JP 2012528567A5 JP 2012504107 A JP2012504107 A JP 2012504107A JP 2012504107 A JP2012504107 A JP 2012504107A JP 2012528567 A5 JP2012528567 A5 JP 2012528567A5
- Authority
- JP
- Japan
- Prior art keywords
- deletion
- nucleotide
- gene
- seq
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012217 deletion Methods 0.000 claims 31
- 230000037430 deletion Effects 0.000 claims 31
- 239000002773 nucleotide Substances 0.000 claims 30
- 125000003729 nucleotide group Chemical group 0.000 claims 30
- 239000000523 sample Substances 0.000 claims 16
- 238000000034 method Methods 0.000 claims 12
- 108700012359 toxins Proteins 0.000 claims 9
- 101150032575 tcdA gene Proteins 0.000 claims 8
- 101150089436 tcdB gene Proteins 0.000 claims 8
- 101150008941 cdtA gene Proteins 0.000 claims 7
- 230000003321 amplification Effects 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 241000193163 Clostridioides difficile Species 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 239000003053 toxin Substances 0.000 claims 5
- 231100000765 toxin Toxicity 0.000 claims 5
- 101710112752 Cytotoxin Proteins 0.000 claims 4
- 231100000599 cytotoxic agent Toxicity 0.000 claims 4
- 239000002619 cytotoxin Substances 0.000 claims 4
- 238000007403 mPCR Methods 0.000 claims 4
- 238000003556 assay Methods 0.000 claims 3
- 239000003269 fluorescent indicator Substances 0.000 claims 3
- 230000003287 optical effect Effects 0.000 claims 3
- 101150076489 B gene Proteins 0.000 claims 2
- 108091093088 Amplicon Proteins 0.000 claims 1
- 101710146739 Enterotoxin Proteins 0.000 claims 1
- 238000011529 RT qPCR Methods 0.000 claims 1
- 101150108363 cdtB gene Proteins 0.000 claims 1
- 239000000147 enterotoxin Substances 0.000 claims 1
- 231100000655 enterotoxin Toxicity 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09157547.2 | 2009-04-07 | ||
| EP09157547 | 2009-04-07 | ||
| PCT/IB2010/051396 WO2010116290A1 (en) | 2009-04-07 | 2010-03-31 | Method for the detection and characterization of a toxinogenic clostridium difficile strain |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012528567A JP2012528567A (ja) | 2012-11-15 |
| JP2012528567A5 true JP2012528567A5 (enExample) | 2013-05-09 |
| JP5859424B2 JP5859424B2 (ja) | 2016-02-10 |
Family
ID=42224104
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012504107A Active JP5859424B2 (ja) | 2009-04-07 | 2010-03-31 | 毒素産生クロストリジウムディフィシル菌の検出及び特性解析方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20120028819A1 (enExample) |
| EP (1) | EP2419527B1 (enExample) |
| JP (1) | JP5859424B2 (enExample) |
| KR (1) | KR101798211B1 (enExample) |
| CN (1) | CN102378817B (enExample) |
| BR (1) | BRPI1006757A2 (enExample) |
| RU (1) | RU2612789C2 (enExample) |
| WO (1) | WO2010116290A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120028819A1 (en) | 2009-04-07 | 2012-02-02 | Koninklijke Philips Electronics N.V. | Method for the detection and characterization of a toxinogenic clostridium difficile strain |
| WO2012087135A1 (en) | 2010-12-22 | 2012-06-28 | Academisch Ziekenhuis Leiden H.O.D.N. Lumc | Genetic markers specific for clostridium difficile ribotypes 027 (nap01/b1; rt 027) and 078 (nap7/8; rt 078) and their use |
| GB201110712D0 (en) | 2011-06-23 | 2011-08-10 | Univ Ulster | Diagnostic methods |
| CN108866169A (zh) * | 2011-07-06 | 2018-11-23 | 探索诊断投资公司 | 病毒和细菌病原体的直接扩增和检测 |
| GB201207998D0 (en) * | 2012-05-08 | 2012-06-20 | Univ Cardiff | A screening method for the detection of clostridium difficle |
| US9133527B2 (en) * | 2012-05-11 | 2015-09-15 | Techlab, Inc. | Cell wall protein CwpV (CD0514) as a diagnostic marker for Clostridium difficile ribotype 027 |
| KR101955329B1 (ko) * | 2012-06-08 | 2019-03-07 | 삼성전자주식회사 | 클로스트리디움 디피실리 균주 검출용 조성물과 키트 및 이를 이용한 검출 방법 |
| CN102952886A (zh) * | 2012-11-28 | 2013-03-06 | 中华人民共和国张家港出入境检验检疫局 | 艰难梭菌肠毒素a、b双重荧光定量pcr检测方法及检测用试剂盒 |
| WO2014164563A1 (en) | 2013-03-13 | 2014-10-09 | Quidel Corporation | Pcr assays and reagents for molecular detection of infectious agents |
| TWI504751B (zh) * | 2013-09-25 | 2015-10-21 | Univ Nat Cheng Kung | 檢測困難梭狀桿菌核醣分型027之序列、技術平台及其方法 |
| FI126289B (en) * | 2014-12-19 | 2016-09-15 | Mobidiag Ltd | Method for detecting the presence of a hypervirulent strain of Clostridium difficile |
| US20190211377A1 (en) * | 2016-12-22 | 2019-07-11 | Roche Molecular Systems, Inc. | Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile |
| JP2021506274A (ja) * | 2017-12-15 | 2021-02-22 | ジェン−プローブ・インコーポレーテッド | 毒素産生Ｃｌｏｓｔｒｉｄｉｕｍ ｄｉｆｆｉｃｉｌｅを検出するための組成物および方法 |
| EP3822370A1 (en) | 2019-11-15 | 2021-05-19 | Curiosity Diagnostics Sp. z o.o. | Method of determining the presence of a hyper-virulent clostridioides difficile strain of the b1/nap1/027 group in a sample |
| CN111705150B (zh) * | 2020-08-19 | 2020-12-25 | 中南大学湘雅医院 | 用于检测艰难梭菌核糖体027型毒力调节基因tcdC的方法、引物和试剂盒 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5298421A (en) | 1990-04-26 | 1994-03-29 | Calgene, Inc. | Plant medium-chain-preferring acyl-ACP thioesterases and related methods |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| WO1997046714A1 (en) | 1996-06-04 | 1997-12-11 | University Of Utah Research Foundation | Monitoring hybridization during pcr |
| FR2812306B1 (fr) * | 2000-07-28 | 2005-01-14 | Gabriel Festoc | Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles |
| JP4999103B2 (ja) * | 2004-10-27 | 2012-08-15 | セフィード | 閉鎖系の多段階核酸増幅反応 |
| US20070026426A1 (en) * | 2005-04-26 | 2007-02-01 | Applera Corporation | System for genetic surveillance and analysis |
| RU2348695C2 (ru) * | 2006-05-23 | 2009-03-10 | Закрытое акционерное общество "Молекулярно-медицинские технологии" | Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа |
| JP5596893B2 (ja) * | 2006-11-10 | 2014-09-24 | キヤノン株式会社 | プローブセット、プローブ固定担体及び遺伝子検査方法 |
| US7687084B2 (en) | 2007-08-06 | 2010-03-30 | Tien Linsheng W | Cool-pet system |
| US9096638B2 (en) | 2007-09-06 | 2015-08-04 | Geneohm Sciences Canada, Inc. | Detection of toxigenic strains of Clostridium difficile |
| WO2009100215A2 (en) * | 2008-02-08 | 2009-08-13 | Mayo Foundation For Medical Education And Research | Detection of clostridium difficile |
| US20120028819A1 (en) | 2009-04-07 | 2012-02-02 | Koninklijke Philips Electronics N.V. | Method for the detection and characterization of a toxinogenic clostridium difficile strain |
-
2010
- 2010-03-31 US US13/262,844 patent/US20120028819A1/en not_active Abandoned
- 2010-03-31 JP JP2012504107A patent/JP5859424B2/ja active Active
- 2010-03-31 CN CN201080014449.3A patent/CN102378817B/zh active Active
- 2010-03-31 KR KR1020117026359A patent/KR101798211B1/ko active Active
- 2010-03-31 BR BRPI1006757-4A patent/BRPI1006757A2/pt not_active Application Discontinuation
- 2010-03-31 EP EP10713524.6A patent/EP2419527B1/en not_active Revoked
- 2010-03-31 WO PCT/IB2010/051396 patent/WO2010116290A1/en not_active Ceased
- 2010-03-31 RU RU2011144853A patent/RU2612789C2/ru active
-
2017
- 2017-06-08 US US15/617,010 patent/US10876174B2/en active Active
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