RU2612789C2 - Способ детектирования и характеризации токсиногенного штамма clostridium difficile - Google Patents

Способ детектирования и характеризации токсиногенного штамма clostridium difficile Download PDF

Info

Publication number
RU2612789C2
RU2612789C2 RU2011144853A RU2011144853A RU2612789C2 RU 2612789 C2 RU2612789 C2 RU 2612789C2 RU 2011144853 A RU2011144853 A RU 2011144853A RU 2011144853 A RU2011144853 A RU 2011144853A RU 2612789 C2 RU2612789 C2 RU 2612789C2
Authority
RU
Russia
Prior art keywords
seq
gene
sample
deletion
deletions
Prior art date
Application number
RU2011144853A
Other languages
English (en)
Russian (ru)
Other versions
RU2011144853A (ru
Inventor
ДЕН БОГААРД Патрик Т. К. ВАН
Астрид Е. ВИССЕР
Original Assignee
Конинклейке Филипс Электроникс Н.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42224104&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RU2612789(C2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Конинклейке Филипс Электроникс Н.В. filed Critical Конинклейке Филипс Электроникс Н.В.
Publication of RU2011144853A publication Critical patent/RU2011144853A/ru
Application granted granted Critical
Publication of RU2612789C2 publication Critical patent/RU2612789C2/ru

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145Clostridium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
RU2011144853A 2009-04-04 2010-03-31 Способ детектирования и характеризации токсиногенного штамма clostridium difficile RU2612789C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09157547.2 2009-04-07
EP09157547 2009-04-07
PCT/IB2010/051396 WO2010116290A1 (en) 2009-04-07 2010-03-31 Method for the detection and characterization of a toxinogenic clostridium difficile strain

Publications (2)

Publication Number Publication Date
RU2011144853A RU2011144853A (ru) 2013-05-20
RU2612789C2 true RU2612789C2 (ru) 2017-03-13

Family

ID=42224104

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2011144853A RU2612789C2 (ru) 2009-04-04 2010-03-31 Способ детектирования и характеризации токсиногенного штамма clostridium difficile

Country Status (8)

Country Link
US (2) US20120028819A1 (enExample)
EP (1) EP2419527B1 (enExample)
JP (1) JP5859424B2 (enExample)
KR (1) KR101798211B1 (enExample)
CN (1) CN102378817B (enExample)
BR (1) BRPI1006757A2 (enExample)
RU (1) RU2612789C2 (enExample)
WO (1) WO2010116290A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2841390C1 (ru) * 2024-11-27 2025-06-06 Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный аграрный университет" (ФГБОУ ВО СПбГАУ) Способ оценки генов токсинов Clostridium perfringens и Clostridioides difficile в консервированных объемистых кормах для крупного рогатого скота

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120028819A1 (en) 2009-04-07 2012-02-02 Koninklijke Philips Electronics N.V. Method for the detection and characterization of a toxinogenic clostridium difficile strain
WO2012087135A1 (en) 2010-12-22 2012-06-28 Academisch Ziekenhuis Leiden H.O.D.N. Lumc Genetic markers specific for clostridium difficile ribotypes 027 (nap01/b1; rt 027) and 078 (nap7/8; rt 078) and their use
GB201110712D0 (en) 2011-06-23 2011-08-10 Univ Ulster Diagnostic methods
CN108866169A (zh) * 2011-07-06 2018-11-23 探索诊断投资公司 病毒和细菌病原体的直接扩增和检测
GB201207998D0 (en) * 2012-05-08 2012-06-20 Univ Cardiff A screening method for the detection of clostridium difficle
US9133527B2 (en) * 2012-05-11 2015-09-15 Techlab, Inc. Cell wall protein CwpV (CD0514) as a diagnostic marker for Clostridium difficile ribotype 027
KR101955329B1 (ko) * 2012-06-08 2019-03-07 삼성전자주식회사 클로스트리디움 디피실리 균주 검출용 조성물과 키트 및 이를 이용한 검출 방법
CN102952886A (zh) * 2012-11-28 2013-03-06 中华人民共和国张家港出入境检验检疫局 艰难梭菌肠毒素a、b双重荧光定量pcr检测方法及检测用试剂盒
WO2014164563A1 (en) 2013-03-13 2014-10-09 Quidel Corporation Pcr assays and reagents for molecular detection of infectious agents
TWI504751B (zh) * 2013-09-25 2015-10-21 Univ Nat Cheng Kung 檢測困難梭狀桿菌核醣分型027之序列、技術平台及其方法
FI126289B (en) * 2014-12-19 2016-09-15 Mobidiag Ltd Method for detecting the presence of a hypervirulent strain of Clostridium difficile
US20190211377A1 (en) * 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
JP2021506274A (ja) * 2017-12-15 2021-02-22 ジェン−プローブ・インコーポレーテッド 毒素産生Clostridium difficileを検出するための組成物および方法
EP3822370A1 (en) 2019-11-15 2021-05-19 Curiosity Diagnostics Sp. z o.o. Method of determining the presence of a hyper-virulent clostridioides difficile strain of the b1/nap1/027 group in a sample
CN111705150B (zh) * 2020-08-19 2020-12-25 中南大学湘雅医院 用于检测艰难梭菌核糖体027型毒力调节基因tcdC的方法、引物和试剂盒

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2348695C2 (ru) * 2006-05-23 2009-03-10 Закрытое акционерное общество "Молекулярно-медицинские технологии" Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа
WO2009030031A1 (en) * 2007-09-06 2009-03-12 Geneohm Sciences Canada, Inc. Detection of toxigenic strains of clostridium difficile

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5298421A (en) 1990-04-26 1994-03-29 Calgene, Inc. Plant medium-chain-preferring acyl-ACP thioesterases and related methods
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
WO1997046714A1 (en) 1996-06-04 1997-12-11 University Of Utah Research Foundation Monitoring hybridization during pcr
FR2812306B1 (fr) * 2000-07-28 2005-01-14 Gabriel Festoc Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles
JP4999103B2 (ja) * 2004-10-27 2012-08-15 セフィード 閉鎖系の多段階核酸増幅反応
US20070026426A1 (en) * 2005-04-26 2007-02-01 Applera Corporation System for genetic surveillance and analysis
JP5596893B2 (ja) * 2006-11-10 2014-09-24 キヤノン株式会社 プローブセット、プローブ固定担体及び遺伝子検査方法
US7687084B2 (en) 2007-08-06 2010-03-30 Tien Linsheng W Cool-pet system
WO2009100215A2 (en) * 2008-02-08 2009-08-13 Mayo Foundation For Medical Education And Research Detection of clostridium difficile
US20120028819A1 (en) 2009-04-07 2012-02-02 Koninklijke Philips Electronics N.V. Method for the detection and characterization of a toxinogenic clostridium difficile strain

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2348695C2 (ru) * 2006-05-23 2009-03-10 Закрытое акционерное общество "Молекулярно-медицинские технологии" Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа
WO2009030031A1 (en) * 2007-09-06 2009-03-12 Geneohm Sciences Canada, Inc. Detection of toxigenic strains of clostridium difficile

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BOUVET P.J. et al., Genetic relatedness of Clostridium difficile isolates from various origins determined by triple-locus sequence analysis based on toxin regulatory genes tcdC, tcdR, and cdtR, J Clin Microbiol. 2008, v.46, no.11, p. 3703-3713. *
CURRY S.R. et al., tcdC genotypes associated with severe TcdC truncation in an epidemic clone and other strains of Clostridium difficile, J Clin Microbiol, 2007, v.45, no.1, p. 215-221. *
MACCANNELL D.R. et al., Molecular analysis of Clostridium difficile PCR ribotype 027 isolates from Eastern and Western Canada, J Clin Microbiol, 2006, v.44, no.6, p. 2147-2152. *
PERSSON S. et al., New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA/cdtB) genes applied to a Danish strain collection, Clin Microbiol Infect, 2008, v.14, no.11, p.1057-1064. *
SPIGAGLIA P. et al., Molecular analysis of the pathogenicity locus and polymorphism in the putative negative regulator of toxin production (TcdC) among Clostridium difficile clinical isolates, J Clin Microbiol, 2002, v.40, no.9, p. 3470-3475. *
SPIGAGLIA P. et al., Molecular analysis of the pathogenicity locus and polymorphism in the putative negative regulator of toxin production (TcdC) among Clostridium difficile clinical isolates, J Clin Microbiol, 2002, v.40, no.9, p. 3470-3475. CURRY S.R. et al., tcdC genotypes associated with severe TcdC truncation in an epidemic clone and other strains of Clostridium difficile, J Clin Microbiol, 2007, v.45, no.1, p. 215-221. BOUVET P.J. et al., Genetic relatedness of Clostridium difficile isolates from various origins determined by triple-locus sequence analysis based on toxin regulatory genes tcdC, tcdR, and cdtR, J Clin Microbiol. 2008, v.46, no.11, p. 3703-3713. PERSSON S. et al., New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA/cdtB) genes applied to a Danish strain collection, Clin Microbiol Infect, 2008, v.14, no.11, p.1057-1064. WOLFF D. et al., Rapid detection of the Clostridium difficile ribotype 0 *
VAN DEN BERG R.J. et al., Characterization of toxin A-negative, toxin B-positive Clostridium difficile isolates from outbreaks in different countries by amplified fragment length polymorphism and PCR ribotyping, J Clin Microbiol, 2004, v.42, no.3, p.1035-1041. *
WOLFF D. et al., Rapid detection of the Clostridium difficile ribotype 027 tcdC gene frame shift mutation at position 117 by real-time PCR and melt curve analysis, Eur J Clin Microbiol Infect Dis, 2009, v.28, no.8, p. 959-962. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2841390C1 (ru) * 2024-11-27 2025-06-06 Федеральное государственное бюджетное образовательное учреждение высшего образования "Санкт-Петербургский государственный аграрный университет" (ФГБОУ ВО СПбГАУ) Способ оценки генов токсинов Clostridium perfringens и Clostridioides difficile в консервированных объемистых кормах для крупного рогатого скота

Also Published As

Publication number Publication date
KR20120005019A (ko) 2012-01-13
EP2419527B1 (en) 2013-06-26
US10876174B2 (en) 2020-12-29
CN102378817A (zh) 2012-03-14
CN102378817B (zh) 2015-09-30
KR101798211B1 (ko) 2017-11-15
US20120028819A1 (en) 2012-02-02
WO2010116290A1 (en) 2010-10-14
JP2012528567A (ja) 2012-11-15
US20180237827A1 (en) 2018-08-23
EP2419527A1 (en) 2012-02-22
BRPI1006757A2 (pt) 2020-03-10
RU2011144853A (ru) 2013-05-20
JP5859424B2 (ja) 2016-02-10

Similar Documents

Publication Publication Date Title
RU2612789C2 (ru) Способ детектирования и характеризации токсиногенного штамма clostridium difficile
EP3320110B1 (en) 16s ribosomal rna universal primers and use thereof in microbiological analysis and diagnostics
JP7055020B2 (ja) 薬剤に対する生物の応答のマイクロ流体測定
EP2271767B1 (en) Amplicon rescue multiplex polymerase chain reaction for amplificaton of multiple targets
CA2840964C (en) Direct amplification and detection of viral and bacterial pathogens
TWI697560B (zh) 用於偵測細菌污染之方法及組合物
CA2895945C (en) Target capture system
Amita et al. Qualitative evaluation of mycobacterial DNA extraction protocols for polymerase chain reaction
US8895240B2 (en) Method, kit and system for collecting and processing samples for DNA and RNA detection
BRPI0517131B1 (pt) Métodos para simplificar ácidos nucléicos microbiais por modificação química de citosinas
WO2009061752A1 (en) Methods for detecting toxigenic microbes
De Felice et al. Latest trends in biosensors powered by nucleic acid isothermal amplification for the diagnosis of joint infections: From sampling to identification towards the point-of-care
Hockman et al. Comparison of multiplex PCR hybridization-based and singleplex real-time PCR-based assays for detection of low prevalence pathogens in spiked samples
US20250290165A1 (en) Viral variant detection
EP1851325B1 (en) Method for detecting viable cells in a sample by using a virus
Mohammed et al. Review on polymerase chain reaction and its diagnostic merit over conventional techniques in animal disease
KR101911017B1 (ko) 타일로렐라 에퀴제니탈리스의 검출용 조성물, 타일로렐라 에퀴제니탈리스 감염에 의한 말전염성자궁염의 진단용 조성물, 타일로렐라 에퀴제니탈리스의 검출방법, 및 타일로렐라 에퀴제니탈리스 감염에 의한 말전염성자궁염의 진단방법
RU2814556C1 (ru) Тест-система для выявления ДНК возбудителя пастереллеза (Pasteurella multocida) в биологическом материале животных и кормах с помощью полимеразной цепной реакции в режиме реального времени
TELLI PRINCIPLES of POLYMERASE CHAIN REACTION TECHNOLOGY an d APPLICATIONS n THE FOOD SCIENCE
WO2016209938A1 (en) Compositions and methods for processing a biological sample
Lindström et al. Detection of Clostridium botulinum by multiplex PCR in foods and feces
De Bruin et al. Molecular detection and typing of Coxiella burnetii
CN120752352A (zh) 可存活细胞检测及其实施方案
EP3169796A1 (en) Methods of detecting trichomonas vaginalis
Kubo et al. Hot cell-direct PCR aimed at specific cell detection