CN102378817B - 用于检测和表征产毒性艰难梭菌菌株的方法 - Google Patents

用于检测和表征产毒性艰难梭菌菌株的方法 Download PDF

Info

Publication number
CN102378817B
CN102378817B CN201080014449.3A CN201080014449A CN102378817B CN 102378817 B CN102378817 B CN 102378817B CN 201080014449 A CN201080014449 A CN 201080014449A CN 102378817 B CN102378817 B CN 102378817B
Authority
CN
China
Prior art keywords
nucleotide
deletion
seq
sample
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201080014449.3A
Other languages
English (en)
Chinese (zh)
Other versions
CN102378817A (zh
Inventor
P.T.C.范登博加尔德
A.E.维塞尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Philips NV
Original Assignee
Koninklijke Philips Electronics NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42224104&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN102378817(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Koninklijke Philips Electronics NV filed Critical Koninklijke Philips Electronics NV
Publication of CN102378817A publication Critical patent/CN102378817A/zh
Application granted granted Critical
Publication of CN102378817B publication Critical patent/CN102378817B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201080014449.3A 2009-04-07 2010-03-31 用于检测和表征产毒性艰难梭菌菌株的方法 Active CN102378817B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09157547 2009-04-07
EP09157547.2 2009-04-07
PCT/IB2010/051396 WO2010116290A1 (en) 2009-04-07 2010-03-31 Method for the detection and characterization of a toxinogenic clostridium difficile strain

Publications (2)

Publication Number Publication Date
CN102378817A CN102378817A (zh) 2012-03-14
CN102378817B true CN102378817B (zh) 2015-09-30

Family

ID=42224104

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080014449.3A Active CN102378817B (zh) 2009-04-07 2010-03-31 用于检测和表征产毒性艰难梭菌菌株的方法

Country Status (8)

Country Link
US (2) US20120028819A1 (enExample)
EP (1) EP2419527B1 (enExample)
JP (1) JP5859424B2 (enExample)
KR (1) KR101798211B1 (enExample)
CN (1) CN102378817B (enExample)
BR (1) BRPI1006757A2 (enExample)
RU (1) RU2612789C2 (enExample)
WO (1) WO2010116290A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120028819A1 (en) 2009-04-07 2012-02-02 Koninklijke Philips Electronics N.V. Method for the detection and characterization of a toxinogenic clostridium difficile strain
WO2012087135A1 (en) 2010-12-22 2012-06-28 Academisch Ziekenhuis Leiden H.O.D.N. Lumc Genetic markers specific for clostridium difficile ribotypes 027 (nap01/b1; rt 027) and 078 (nap7/8; rt 078) and their use
GB201110712D0 (en) 2011-06-23 2011-08-10 Univ Ulster Diagnostic methods
CN108866169A (zh) 2011-07-06 2018-11-23 探索诊断投资公司 病毒和细菌病原体的直接扩增和检测
GB201207998D0 (en) * 2012-05-08 2012-06-20 Univ Cardiff A screening method for the detection of clostridium difficle
US9133527B2 (en) * 2012-05-11 2015-09-15 Techlab, Inc. Cell wall protein CwpV (CD0514) as a diagnostic marker for Clostridium difficile ribotype 027
KR101955329B1 (ko) 2012-06-08 2019-03-07 삼성전자주식회사 클로스트리디움 디피실리 균주 검출용 조성물과 키트 및 이를 이용한 검출 방법
CN102952886A (zh) * 2012-11-28 2013-03-06 中华人民共和国张家港出入境检验检疫局 艰难梭菌肠毒素a、b双重荧光定量pcr检测方法及检测用试剂盒
WO2014164563A1 (en) 2013-03-13 2014-10-09 Quidel Corporation Pcr assays and reagents for molecular detection of infectious agents
TWI504751B (zh) * 2013-09-25 2015-10-21 Univ Nat Cheng Kung 檢測困難梭狀桿菌核醣分型027之序列、技術平台及其方法
FI126289B (en) 2014-12-19 2016-09-15 Mobidiag Ltd Method for detecting the presence of a hypervirulent strain of Clostridium difficile
US20190211377A1 (en) * 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
WO2019118735A1 (en) * 2017-12-15 2019-06-20 Gen-Probe Incorporated Compositions and methods for detecting toxigenic clostridium difficile
EP3822370A1 (en) 2019-11-15 2021-05-19 Curiosity Diagnostics Sp. z o.o. Method of determining the presence of a hyper-virulent clostridioides difficile strain of the b1/nap1/027 group in a sample
CN111705150B (zh) * 2020-08-19 2020-12-25 中南大学湘雅医院 用于检测艰难梭菌核糖体027型毒力调节基因tcdC的方法、引物和试剂盒

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5298421A (en) 1990-04-26 1994-03-29 Calgene, Inc. Plant medium-chain-preferring acyl-ACP thioesterases and related methods
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
FR2812306B1 (fr) * 2000-07-28 2005-01-14 Gabriel Festoc Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles
KR20070073935A (ko) * 2004-10-27 2007-07-10 세페이드 폐쇄 시스템의 다단계 핵산 증폭 반응
US20070026426A1 (en) * 2005-04-26 2007-02-01 Applera Corporation System for genetic surveillance and analysis
RU2348695C2 (ru) 2006-05-23 2009-03-10 Закрытое акционерное общество "Молекулярно-медицинские технологии" Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа
JP5596893B2 (ja) 2006-11-10 2014-09-24 キヤノン株式会社 プローブセット、プローブ固定担体及び遺伝子検査方法
US7687084B2 (en) 2007-08-06 2010-03-30 Tien Linsheng W Cool-pet system
US9096638B2 (en) 2007-09-06 2015-08-04 Geneohm Sciences Canada, Inc. Detection of toxigenic strains of Clostridium difficile
JP5665548B2 (ja) * 2008-02-08 2015-02-04 メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ クロストリジウム・ディフィシレの検出
US20120028819A1 (en) 2009-04-07 2012-02-02 Koninklijke Philips Electronics N.V. Method for the detection and characterization of a toxinogenic clostridium difficile strain

Also Published As

Publication number Publication date
RU2011144853A (ru) 2013-05-20
BRPI1006757A2 (pt) 2020-03-10
US10876174B2 (en) 2020-12-29
EP2419527B1 (en) 2013-06-26
JP5859424B2 (ja) 2016-02-10
CN102378817A (zh) 2012-03-14
JP2012528567A (ja) 2012-11-15
KR101798211B1 (ko) 2017-11-15
RU2612789C2 (ru) 2017-03-13
US20120028819A1 (en) 2012-02-02
EP2419527A1 (en) 2012-02-22
WO2010116290A1 (en) 2010-10-14
US20180237827A1 (en) 2018-08-23
KR20120005019A (ko) 2012-01-13

Similar Documents

Publication Publication Date Title
CN102378817B (zh) 用于检测和表征产毒性艰难梭菌菌株的方法
EP2935613B1 (en) Target capture system
AU2013312191C1 (en) Multiple amplification cycle detection
CN102066573A (zh) 用于扩增多个靶标的扩增子拯救多重聚合酶链式反应
TW201525145A (zh) 用於偵測細菌污染之方法及組合物
EP2902506B1 (en) Detection of listeria species in food and environmental samples, methods and compositions thereof
CN102329880B (zh) 结核菌检测用的引物和探针以及使用它们检测人型结核菌的方法
BRPI0517131B1 (pt) Métodos para simplificar ácidos nucléicos microbiais por modificação química de citosinas
US20110287965A1 (en) Methods and compositions to detect clostridium difficile
US9234248B2 (en) Simultaneous quantitative multiple primer detection of Clostridium difficile
EP2889382A1 (en) Oligonucleotides, use thereof, methods and a kit for detection of DNA Borrelia burgdorferi, Bartonella henselae, Babesia divergens, Anaplasma phagocytophilum
RU2289627C2 (ru) СПОСОБ И НАБОР ДЛЯ ОБНАРУЖЕНИЯ ДНК БАКТЕРИИ ЗАБОЛЕВАНИЯ ЛАЙМСКОГО БОРРЕЛИОЗА-Borrelia burgdorferi
RU2346045C1 (ru) ОЛИГОНУКЛЕОТИДНЫЕ ПРАЙМЕРЫ ДЛЯ ИДЕНТИФИКАЦИИ Coccidioides posadasii
KR101911017B1 (ko) 타일로렐라 에퀴제니탈리스의 검출용 조성물, 타일로렐라 에퀴제니탈리스 감염에 의한 말전염성자궁염의 진단용 조성물, 타일로렐라 에퀴제니탈리스의 검출방법, 및 타일로렐라 에퀴제니탈리스 감염에 의한 말전염성자궁염의 진단방법
AU2014400858B2 (en) Methods of detecting Trichomonas vaginalis
TELLI PRINCIPLES of POLYMERASE CHAIN REACTION TECHNOLOGY an d APPLICATIONS n THE FOOD SCIENCE
WO1999005325A1 (en) Methods and compositions for determining species of bacteria and fungi
CN106480219A (zh) 一种畜禽葡萄球菌大环内酯类药物耐药基因多重pcr检测试剂盒及使用方法
RU2397251C1 (ru) Набор для детекции возбудителя легионеллеза в биологическом материале и объектах окружающей среды
Wu et al. Identification and subspeciation of Mycobacterium avium complex isolates by PCR amplification of a fibronectin-attachment protein gene element.
De Bruin et al. Molecular detection and typing of Coxiella burnetii
Han et al. Enteritis caused by type a Clostridium perfringens producing α-toxin in a dog
KR20080106616A (ko) 변성고성능액체크로마토그래피를 이용한 결핵균 판별방법
JP2008136421A (ja) 核酸増幅方法および核酸増幅キット

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant