JP2012525591A - プログラム可能な電気泳動ノッチフィルターシステムと方法 - Google Patents
プログラム可能な電気泳動ノッチフィルターシステムと方法 Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44713—Particularly adapted electric power supply
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- Electrostatic Separation (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17308709P | 2009-04-27 | 2009-04-27 | |
| US61/173,087 | 2009-04-27 | ||
| PCT/US2010/032598 WO2010126897A1 (en) | 2009-04-27 | 2010-04-27 | Programmable electrophoretic notch filter systems and methods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012525591A true JP2012525591A (ja) | 2012-10-22 |
| JP2012525591A5 JP2012525591A5 (https=) | 2013-08-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012508597A Pending JP2012525591A (ja) | 2009-04-27 | 2010-04-27 | プログラム可能な電気泳動ノッチフィルターシステムと方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8926817B2 (https=) |
| EP (1) | EP2425216B1 (https=) |
| JP (1) | JP2012525591A (https=) |
| CN (1) | CN102439398A (https=) |
| WO (1) | WO2010126897A1 (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015133190A1 (ja) * | 2014-03-07 | 2015-09-11 | テルモ株式会社 | ゲル電気泳動装置およびゲル電気泳動方法 |
| JP2020508472A (ja) * | 2017-02-21 | 2020-03-19 | バイオプティック インコーポレイテッドＢｉｏｐｔｉｃ， Ｉｎｃ． | 使い捨てマルチチャネルバイオ分析カートリッジ、及びそれを用いてバイオ分析を実施するためのキャピラリー電気泳動システム |
| JP2023139750A (ja) * | 2022-03-22 | 2023-10-04 | 株式会社東芝 | 抗酸化剤を用いたｆｅｔセンサ |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2014025392A1 (en) * | 2012-08-09 | 2014-02-13 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for preparing biological specimens for microscopic analysis |
| WO2014155559A1 (ja) * | 2013-03-27 | 2014-10-02 | 株式会社安川電機 | ノッチフィルタ、外力推定器、モータ制御装置およびロボットシステム |
| CA2936617C (en) * | 2014-01-17 | 2022-11-29 | Coastal Genomics Inc. | Cassettes for use in automated parallel electrophoretic assays and methods for manufacturing and using same |
| US10598631B2 (en) * | 2014-03-28 | 2020-03-24 | Ge Healthcare Bio-Sciences Ab | Electrophoresis separation method |
| EP3126037B1 (en) * | 2014-04-04 | 2020-11-18 | Massachusetts Institute Of Technology | Active transport of charged molecules into, within, and/or from charged matrices |
| CA3159833A1 (en) * | 2014-05-21 | 2015-11-26 | Unchained Labs | Systems and methods for exchange of buffer solutions |
| US10564123B2 (en) | 2014-05-25 | 2020-02-18 | United Arab Emirates University | Bioreactor system and method of operating same for cellular composition identification and quantification |
| US10436772B2 (en) | 2014-08-25 | 2019-10-08 | United Arab Emirates University | Method and system for counting white blood cells electrically |
| US10078067B2 (en) | 2014-08-25 | 2018-09-18 | United Arab Emirates University | Apparatus and method for detection and quantification of biological and chemical analytes |
| US10690627B2 (en) * | 2014-10-22 | 2020-06-23 | IntegenX, Inc. | Systems and methods for sample preparation, processing and analysis |
| WO2016175864A1 (en) | 2015-04-30 | 2016-11-03 | Hewlett-Packard Development Company, L.P. | Target constituent location and discharge |
| US20170227493A1 (en) | 2015-05-22 | 2017-08-10 | Bioptic, Inc. | Disposable multi-channel bio-analysis cartridge and capillary electrophoresis system for conducting bio-analysis using same |
| CN108026494A (zh) | 2015-06-05 | 2018-05-11 | 米罗库鲁斯公司 | 限制蒸发和表面结垢的空气基质数字微流控装置和方法 |
| US10695762B2 (en) | 2015-06-05 | 2020-06-30 | Miroculus Inc. | Evaporation management in digital microfluidic devices |
| KR101845627B1 (ko) | 2016-10-04 | 2018-04-04 | 울산과학기술원 | 전기삼투 및 유전영동을 이용한 전기화학적 바이오 센서 및 그를 포함하는 공기 중의 바이오 입자 감지 장치 |
| CN110383061A (zh) | 2016-12-28 | 2019-10-25 | 米罗库鲁斯公司 | 数字微流控设备和方法 |
| EP3658908B1 (en) | 2017-07-24 | 2025-11-12 | Integra Biosciences AG | Digital microfluidics systems and methods with integrated plasma collection device |
| CA3073058A1 (en) | 2017-09-01 | 2019-03-07 | Miroculus Inc. | Digital microfluidics devices and methods of using them |
| WO2019226919A1 (en) | 2018-05-23 | 2019-11-28 | Miroculus Inc. | Control of evaporation in digital microfluidics |
| EP3917670A4 (en) | 2019-01-31 | 2022-11-02 | Miroculus Inc. | ANTIFOULING COMPOSITIONS AND METHODS FOR HANDLING AND TREATING ENCAPSULATED DROPLET |
| US12582991B2 (en) | 2019-02-28 | 2026-03-24 | Integra Biosciences Ag | Digital microfluidics devices and methods of using them |
| CN114206499B (zh) | 2019-04-08 | 2024-10-08 | 米罗库鲁斯公司 | 多盒式数字微流控装置和使用方法 |
| CN110564719B (zh) * | 2019-08-20 | 2023-10-13 | 天津普瑞思生物科技有限公司 | 一种dna提纯方法以及专用设备 |
| KR102472151B1 (ko) * | 2020-12-24 | 2022-11-28 | 국민대학교산학협력단 | 탈부착형 생체입자 정제장치 및 이를 사용한 생체입자 정제방법 |
| US11772093B2 (en) | 2022-01-12 | 2023-10-03 | Miroculus Inc. | Methods of mechanical microfluidic manipulation |
| WO2025111055A1 (en) * | 2023-11-22 | 2025-05-30 | Sage Science, Inc. | Systems, devices, and methods for performing preparative electrophoresis |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4455370A (en) * | 1982-05-17 | 1984-06-19 | E. I. Du Pont De Nemours And Company | Transferring separated components in gel electrophoresis via nylon membrane |
| JPS6098348A (ja) * | 1983-10-17 | 1985-06-01 | カ−ル シユライヒヤ− ウント シユ−ル ゲ−エムベ−ハ− ウント コンパニ カ−ゲ− | 電気的に荷電された巨大分子を電気溶離するための方法と装置 |
| JPH07508096A (ja) * | 1992-06-17 | 1995-09-07 | ベックマン インスツルメンツ インコーポレーテッド | 時間とともに変化する場の強さを利用する毛管電気泳動 |
| JP2003514829A (ja) * | 1999-11-15 | 2003-04-22 | プロテオム システムズ リミテッド | 多室電気泳動 |
| JP2003207484A (ja) * | 2001-11-08 | 2003-07-25 | Robert Bosch Gmbh | ガス測定フィーラ |
| JP2005535895A (ja) * | 2002-08-17 | 2005-11-24 | パライテック エルティディ | 光透過を検出するための光学アセンブリおよび方法 |
| WO2008041718A1 (fr) * | 2006-10-04 | 2008-04-10 | National University Corporation Hokkaido University | Micropuce, et dispositif d'électrophorèse de micropuce |
| WO2008075563A1 (ja) * | 2006-12-21 | 2008-06-26 | Panasonic Corporation | キャピラリーユニットおよびキャピラリー電気泳動方法 |
| JP2008530524A (ja) * | 2005-02-08 | 2008-08-07 | ラブ９０１ リミテッド | 分析器械 |
| US20090090630A1 (en) * | 1999-08-31 | 2009-04-09 | Changsheng Liu | Automated parallel capillary electrophoresis system with hydrodynamic sample injection |
| WO2009046526A1 (en) * | 2007-10-09 | 2009-04-16 | Dalhousie University | Apparatus for purifying molecules |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3271198A (en) | 1959-12-30 | 1966-09-06 | Ibm | Electrolytic semiconductor photocell |
| EP0155833A3 (en) * | 1984-03-15 | 1988-07-27 | Fuji Photo Film Co., Ltd. | Element for electrophoresis |
| JPS613046A (ja) * | 1984-06-18 | 1986-01-09 | Hitachi Ltd | 電気泳動装置 |
| US5217592A (en) | 1985-09-26 | 1993-06-08 | Jones Kenneth W | Electrophoresis and vacuum molecular transfer apparatus |
| JPS62184343A (ja) | 1986-02-07 | 1987-08-12 | Fuji Photo Film Co Ltd | 電気泳動装置 |
| US5066382A (en) * | 1990-01-25 | 1991-11-19 | Spectra-Physics, Inc. | Thermal control for capillary electrophoresis apparatus |
| US5373197A (en) * | 1992-11-02 | 1994-12-13 | Labconco Corporation | High voltage dual output laboratory power supply |
| CN1092524A (zh) * | 1993-03-09 | 1994-09-21 | 刘田民 | 多室连续流式电泳仪 |
| US5449446A (en) | 1994-03-09 | 1995-09-12 | Verma; Sumeet | Multi-purpose electrophoresis apparatus |
| US6399023B1 (en) | 1996-04-16 | 2002-06-04 | Caliper Technologies Corp. | Analytical system and method |
| US5810989A (en) | 1997-09-04 | 1998-09-22 | Motorola, Inc. | Photoelectric synthesis of DNA or protein probe arrays |
| AU2021099A (en) | 1997-12-30 | 1999-07-19 | Caliper Technologies Corporation | Software for the display of chromatographic separation data |
| US6167910B1 (en) | 1998-01-20 | 2001-01-02 | Caliper Technologies Corp. | Multi-layer microfluidic devices |
| US6494614B1 (en) | 1998-07-27 | 2002-12-17 | Battelle Memorial Institute | Laminated microchannel devices, mixing units and method of making same |
| DE60011390T2 (de) | 1999-01-28 | 2004-10-14 | Université De Genève | Verfahren und vorrichtung zur identifikation oder karakterisierung von polypeptiden |
| US7150994B2 (en) | 1999-03-03 | 2006-12-19 | Symyx Technologies, Inc. | Parallel flow process optimization reactor |
| US20020008058A1 (en) | 1999-06-03 | 2002-01-24 | Kerry D. Nugent | Tapered bore column for high performance liquid chromatography |
| US6811668B1 (en) | 1999-06-22 | 2004-11-02 | Caliper Life Sciences, Inc. | Apparatus for the operation of a microfluidic device |
| CA2375034C (en) | 1999-07-26 | 2012-01-03 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health | Method and device for analysis of biological specimens |
| US6495104B1 (en) | 1999-08-19 | 2002-12-17 | Caliper Technologies Corp. | Indicator components for microfluidic systems |
| US6455007B1 (en) | 2000-06-13 | 2002-09-24 | Symyx Technologies, Inc. | Apparatus and method for testing compositions in contact with a porous medium |
| US7270730B2 (en) | 2000-08-04 | 2007-09-18 | Essen Instruments, Inc. | High-throughput electrophysiological measurement system |
| US20020122747A1 (en) | 2000-09-19 | 2002-09-05 | Mingqi Zhao | Integrated microdevices for conducting chemical operations |
| US6716629B2 (en) | 2000-10-10 | 2004-04-06 | Biotrove, Inc. | Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof |
| WO2002052098A1 (en) | 2000-12-26 | 2002-07-04 | Mitsubishi Materials Corporation | Metal-coated fiber and electroconductive composition comprising the same, and method for production thereof and use thereof |
| US6998251B2 (en) | 2001-01-12 | 2006-02-14 | Syngenta Participations Ag | Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics |
| CA2434699A1 (en) | 2001-01-17 | 2002-10-17 | Randall W. Nelson | An integrated high throughput system for the analysis of biomolecules |
| US6919046B2 (en) | 2001-06-07 | 2005-07-19 | Nanostream, Inc. | Microfluidic analytical devices and methods |
| GB0120131D0 (en) | 2001-08-17 | 2001-10-10 | Micromass Ltd | Maldi target plate |
| GB0122200D0 (en) | 2001-09-14 | 2001-10-31 | James Peter | Concentration of protein and/or peptide samples |
| WO2003062815A1 (en) * | 2002-01-18 | 2003-07-31 | Biocal Technology, Inc. | Multi-segment cartridge for bio-separation with multiplexed fluorescence detection |
| US7622083B2 (en) * | 2002-01-28 | 2009-11-24 | Biocal Technology, Inc. | Multi-capillary electrophoresis cartridge interface mechanism |
| US7090757B2 (en) | 2002-02-15 | 2006-08-15 | Ut-Battelle Llc | Photoelectrochemical molecular comb |
| US7166441B2 (en) | 2002-03-12 | 2007-01-23 | Perseptive Biosystems Inc. | Method and apparatus for the identification and quantification of biomolecules |
| US7419784B2 (en) | 2002-04-02 | 2008-09-02 | Dubrow Robert S | Methods, systems and apparatus for separation and isolation of one or more sample components of a sample biological material |
| US20050000811A1 (en) | 2003-05-06 | 2005-01-06 | Janos Luka | Electrophoretically enhanced methods |
| US7824623B2 (en) | 2003-06-24 | 2010-11-02 | Millipore Corporation | Multifunctional vacuum manifold |
| WO2004113924A2 (en) | 2003-06-25 | 2004-12-29 | Charuk Jeffrey H M | Method and system for generating and capturing peptides on a target surface |
| EP1676292A4 (en) | 2003-10-10 | 2009-01-07 | Protein Discovery Inc | METHOD AND DEVICES FOR CONCENTRATING AND CLEANING ANALYTES FOR CHEMICAL ANALYSIS USING MATRIX SUPPORTED LASER DESORPTION / IONIZATION (MALDI) MASS SPECTROMETRY (MS) |
| US6953928B2 (en) | 2003-10-31 | 2005-10-11 | Applera Corporation | Ion source and methods for MALDI mass spectrometry |
| US7030373B2 (en) | 2003-12-19 | 2006-04-18 | Applera Corporation | MALDI plate construction with grid |
| CA2567537A1 (en) | 2004-05-21 | 2005-12-01 | Caliper Life Sciences, Inc. | Automated system for handling microfluidic devices |
| AU2006235320A1 (en) | 2005-04-05 | 2006-10-19 | Protein Discovery, Inc. | Improved methods and devices for concentration and fractionation of analytes for chemical analysis including Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry (MS) |
| EP1963480A2 (en) | 2005-12-08 | 2008-09-03 | Protein Discovery, Inc. | Methods and devices for concentration and fractionation of analytes for chemical analysis |
| CN100412540C (zh) * | 2005-12-15 | 2008-08-20 | 西安工业学院 | 电泳梯度凝胶胶片的全自动制作装置及制作方法 |
-
2010
- 2010-04-27 JP JP2012508597A patent/JP2012525591A/ja active Pending
- 2010-04-27 WO PCT/US2010/032598 patent/WO2010126897A1/en not_active Ceased
- 2010-04-27 CN CN2010800188453A patent/CN102439398A/zh active Pending
- 2010-04-27 EP EP10770207.8A patent/EP2425216B1/en active Active
- 2010-12-14 US US12/967,890 patent/US8926817B2/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4455370A (en) * | 1982-05-17 | 1984-06-19 | E. I. Du Pont De Nemours And Company | Transferring separated components in gel electrophoresis via nylon membrane |
| JPS6098348A (ja) * | 1983-10-17 | 1985-06-01 | カ−ル シユライヒヤ− ウント シユ−ル ゲ−エムベ−ハ− ウント コンパニ カ−ゲ− | 電気的に荷電された巨大分子を電気溶離するための方法と装置 |
| JPH07508096A (ja) * | 1992-06-17 | 1995-09-07 | ベックマン インスツルメンツ インコーポレーテッド | 時間とともに変化する場の強さを利用する毛管電気泳動 |
| US20090090630A1 (en) * | 1999-08-31 | 2009-04-09 | Changsheng Liu | Automated parallel capillary electrophoresis system with hydrodynamic sample injection |
| JP2003514829A (ja) * | 1999-11-15 | 2003-04-22 | プロテオム システムズ リミテッド | 多室電気泳動 |
| JP2003207484A (ja) * | 2001-11-08 | 2003-07-25 | Robert Bosch Gmbh | ガス測定フィーラ |
| JP2005535895A (ja) * | 2002-08-17 | 2005-11-24 | パライテック エルティディ | 光透過を検出するための光学アセンブリおよび方法 |
| JP2008530524A (ja) * | 2005-02-08 | 2008-08-07 | ラブ９０１ リミテッド | 分析器械 |
| WO2008041718A1 (fr) * | 2006-10-04 | 2008-04-10 | National University Corporation Hokkaido University | Micropuce, et dispositif d'électrophorèse de micropuce |
| WO2008075563A1 (ja) * | 2006-12-21 | 2008-06-26 | Panasonic Corporation | キャピラリーユニットおよびキャピラリー電気泳動方法 |
| WO2009046526A1 (en) * | 2007-10-09 | 2009-04-16 | Dalhousie University | Apparatus for purifying molecules |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015133190A1 (ja) * | 2014-03-07 | 2015-09-11 | テルモ株式会社 | ゲル電気泳動装置およびゲル電気泳動方法 |
| JP2020508472A (ja) * | 2017-02-21 | 2020-03-19 | バイオプティック インコーポレイテッドＢｉｏｐｔｉｃ， Ｉｎｃ． | 使い捨てマルチチャネルバイオ分析カートリッジ、及びそれを用いてバイオ分析を実施するためのキャピラリー電気泳動システム |
| JP2023139750A (ja) * | 2022-03-22 | 2023-10-04 | 株式会社東芝 | 抗酸化剤を用いたｆｅｔセンサ |
Also Published As
| Publication number | Publication date |
|---|---|
| US20110220501A1 (en) | 2011-09-15 |
| US8926817B2 (en) | 2015-01-06 |
| CN102439398A (zh) | 2012-05-02 |
| EP2425216A1 (en) | 2012-03-07 |
| WO2010126897A1 (en) | 2010-11-04 |
| EP2425216B1 (en) | 2019-10-23 |
| EP2425216A4 (en) | 2013-10-09 |
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