JP2012521195A - 多価不飽和脂肪酸シンターゼの核酸分子およびポリペプチド、組成物、ならびにそれらの作製方法および使用 - Google Patents

多価不飽和脂肪酸シンターゼの核酸分子およびポリペプチド、組成物、ならびにそれらの作製方法および使用 Download PDF

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JP2012521195A
JP2012521195A JP2012501000A JP2012501000A JP2012521195A JP 2012521195 A JP2012521195 A JP 2012521195A JP 2012501000 A JP2012501000 A JP 2012501000A JP 2012501000 A JP2012501000 A JP 2012501000A JP 2012521195 A JP2012521195 A JP 2012521195A
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polypeptide
activity
nucleic acid
acid molecule
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カーク イー． アプト
レスリー リヒター
デイビッド シンプソン
ロス ザークレ
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マーテック バイオサイエンシーズ コーポレーション
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JP2012501000A 2009-03-19 2010-03-19 多価不飽和脂肪酸シンターゼの核酸分子およびポリペプチド、組成物、ならびにそれらの作製方法および使用 Withdrawn JP2012521195A (ja)

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US16174209P 2009-03-19 2009-03-19
US61/161,742 2009-03-19
US29646010P 2010-01-19 2010-01-19
US61/296,460 2010-01-19
PCT/US2010/028009 WO2010108114A2 (en) 2009-03-19 2010-03-19 Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof

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JP2015156630A Active JP6432867B2 (ja) 2009-03-19 2015-08-07 多価不飽和脂肪酸シンターゼの核酸分子およびポリペプチド、組成物、ならびにそれらの作製方法および使用
JP2017179405A Expired - Fee Related JP6659044B2 (ja) 2009-03-19 2017-09-19 多価不飽和脂肪酸シンターゼの核酸分子およびポリペプチド、組成物、ならびにそれらの作製方法および使用

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JP2017179405A Expired - Fee Related JP6659044B2 (ja) 2009-03-19 2017-09-19 多価不飽和脂肪酸シンターゼの核酸分子およびポリペプチド、組成物、ならびにそれらの作製方法および使用

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016537985A (ja) * 2013-11-26 2016-12-08 ダウ アグロサイエンシィズ エルエルシー スラウストキトリドｐｕｆａシンターゼによる油糧種子作物におけるオメガ−３長鎖多価不飽和脂肪酸の産生
JP2019514382A (ja) * 2016-05-12 2019-06-06 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． 微細藻類中でのオメガ−３多価不飽和脂肪酸産生を増加させる方法

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
KR20070073986A (ko) 2000-01-28 2007-07-10 마텍 바이오싸이언스스 코포레이션 발효기 내에서 진핵 미생물의 고밀도 배양에 의한고도불포화 지방산을 함유하는 지질의 증진된 생산 방법
WO2005120174A2 (en) * 2004-05-24 2005-12-22 Interactive Research Institute For Health Affairs Omega-3 fatty acid compositions with honey
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
US8940884B2 (en) 2009-03-19 2015-01-27 Dsm Ip Assets B.V. Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof
US9924733B2 (en) 2010-01-19 2018-03-27 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
TW201144442A (en) * 2010-05-17 2011-12-16 Dow Agrosciences Llc Production of DHA and other LC-PUFAs in plants
PT2683824T (pt) 2011-03-07 2018-06-07 Dsm Nutritional Products Ag Modificação de microorganismos thraustochytrid
AU2015252107B2 (en) * 2011-03-07 2018-01-18 Dsm Nutritional Products Ag Engineering thraustochytrid microorganisms
AU2012285806B2 (en) 2011-07-21 2017-09-14 Dsm Ip Assets B.V. Fatty acid compositions
US20140323569A1 (en) * 2011-07-21 2014-10-30 Krishna Raman Microbial oils enriched in polyunsaturated fatty acids
ES2721269T3 (es) 2014-01-28 2019-07-30 Dsm Ip Assets Bv Factores para la producción y acumulación de ácidos grasos poliinsaturados (PUFA) obtenidos con PUFA sintasas
AU2016228545A1 (en) * 2015-03-12 2017-09-21 Synthetic Genomics. Inc. Microorganisms for fatty acid production using elongase and desaturase enzymes
CN107846857B (zh) 2015-06-06 2021-07-02 帝斯曼知识产权资产管理有限公司 使用新模块二十二碳六烯酸(dha)合酶生成多不饱和脂肪酸(pufas)
KR102319845B1 (ko) 2016-07-13 2021-11-01 디에스엠 아이피 어셋츠 비.브이. 조류 숙주 세포에 대한 crispr-cas 시스템
US11248244B2 (en) 2017-05-10 2022-02-15 Nanjing University Of Technology Schizochytrium limacinum strain, building method therefor and application thereof
WO2018219171A1 (zh) * 2017-05-31 2018-12-06 厦门汇盛生物有限公司 一株生产dha和epa的细菌、该细菌基因组中的6个基因片段及它们的应用
CN107384806A (zh) * 2017-08-28 2017-11-24 厦门大学 一种基于同源重组的裂殖壶菌基因表达系统的构建及应用
BR112021002300A2 (pt) 2018-08-10 2021-05-04 Kyowa Hakko Bio Co., Ltd microrganismo que produz ácido eicosapentaenoico e método para a produção de ácido eicosapentaenoico
US20250129395A1 (en) 2022-01-25 2025-04-24 Dsm Ip Assets B.V. Media refinement and nutrient feeding approaches to increase polyunsaturated fatty acid production
WO2025238190A1 (en) 2024-05-15 2025-11-20 Dsm Ip Assets B.V. Spray-dried powders having high pufa content

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050273883A1 (en) * 1999-01-14 2005-12-08 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US20080044871A1 (en) * 1999-01-14 2008-02-21 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof

Family Cites Families (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4762785A (en) 1982-08-12 1988-08-09 Calgene, Inc. Novel method and compositions for introducting alien DNA in vivo
ATE52800T1 (de) 1983-01-13 1990-06-15 Max Planck Gesellschaft Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren.
US6051757A (en) 1983-01-14 2000-04-18 Washington University Regeneration of plants containing genetically engineered T-DNA
WO1984002919A1 (en) 1983-01-17 1984-08-02 Monsanto Co Plasmids for transforming plant cells
NL8300699A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
GB8317248D0 (en) 1983-06-24 1983-07-27 Wyeth John & Brother Ltd Fat compositions
NL8401048A (nl) 1984-04-03 1985-11-01 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van eenzaadlobbige planten.
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
EP0265502A1 (en) 1986-04-30 1988-05-04 Boyce Thompson Institute For Plant Research, Inc. Electric field mediated dna transformation of plant cells and organelles
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5177010A (en) 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0267159A3 (de) 1986-11-07 1990-05-02 Ciba-Geigy Ag Verfahren zur genetischen Modifikation monokotyler Pflanzen
SE455438B (sv) 1986-11-24 1988-07-11 Aga Ab Sett att senka en brennares flamtemperatur samt brennare med munstycken for oxygen resp brensle
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5246841A (en) 1986-12-26 1993-09-21 Sagami Chemical Research Center Microbial process for production of eicosapentaenoic acid
ATE125418T1 (de) 1987-05-20 1995-08-15 Ciba Geigy Ag Zeamays-pflanzen und transgenetische zeamays- pflanzen, die aus protoplasten oder aus von protoplasten erhaltenen zellen regeneriert wurden.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
DE69133512T2 (de) 1990-11-23 2006-09-28 Bayer Bioscience N.V. Verfahren zur Transformation monokotyler Pflanzen
US5601860A (en) 1990-11-30 1997-02-11 American Home Products Corporation Corandomized fat compositions for infant formulas
US5141141A (en) 1990-12-21 1992-08-25 Nanette Leone Bag for use on the body
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
US5639790A (en) 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
JPH07501701A (ja) 1991-12-04 1995-02-23 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 植物由来の脂肪酸不飽和化酵素遺伝子
JPH07505531A (ja) 1992-04-15 1995-06-22 プラント・ジェネティック・システムズ・エヌ・ブイ 単子葉植物細胞の形質転換法
US5683898A (en) 1992-05-15 1997-11-04 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
EP0594868A4 (en) 1992-05-15 1997-01-02 Sagami Chem Res Gene which codes for eicosapentaenoic acid synthetase group and process for producing eicosapentaenoic acid
US5798259A (en) 1992-05-15 1998-08-25 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
DE69334225D1 (de) 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US6372965B1 (en) 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
US5469976A (en) 1993-04-30 1995-11-28 Burchell; James R. Shelf allocation and management system
DE69404385T2 (de) 1993-06-04 1998-02-19 Cavis Srl Trägheitsschalter zur Ausschaltung der Batterie eines Kraftfahrzeuges
DE4323727A1 (de) 1993-07-15 1995-03-09 Boehringer Mannheim Gmbh Verfahren zur Identifizierung von menschlichen und tierischen Zellen mit der Fähigkeit zu unbegrenzter Proliferation oder zur Tumorbildung
US5672491A (en) 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
EP0831149A4 (en) 1995-01-13 2000-12-06 Sagami Chem Res GENES CODING FOR THE GROUP OF ENZYMES FOR BIOSYNTHESIS OF EICOSAPENTIC ACID AND METHOD FOR PRODUCING EICOSAPENTIC ACID
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
US6582941B1 (en) 1995-04-17 2003-06-24 Japan As Represented By Director-General Of Agency Of Industrial Science And Technology Microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
CA2234656C (en) 1995-10-13 2008-01-08 Dow Agrosciences Llc Modified bacillus thuringiensis gene for lepidopteran control in plants
AR006830A1 (es) 1996-04-26 1999-09-29 Du Pont Aceite de soja con alta estabilidad oxidativa
EP0913473A4 (en) 1996-07-10 2000-12-06 Sagami Chem Res METHOD FOR PRODUCING EICOSAPENTIC ACID BY GENETIC RECOMBINATION
US6033883A (en) 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
AU745551B2 (en) 1997-03-27 2002-03-21 Bristol-Myers Squibb Company Use of docosahexanoic acid and arachidonic acid enhancing the growth of preterm infants
SA98190002B1 (ar) 1997-03-28 2006-06-20 ذي يونيفيرسيتي اوف تننيسي ريسيرش كوربوريشن استخدام أحماض دهنية تحتوي على عدة روابط غير مشبعة polyunsaturated fatty acids لتقليل حدوث الالتهاب المعوي القولوني الناخر incidence of necrotizing enterocolitis
BR9808506A (pt) 1997-04-11 2000-05-23 Calgene Llc Métodos e composições para sìntese de ácidos graxos poliinsaturados de cadeia longa em plantas
US6051754A (en) 1997-04-11 2000-04-18 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
CA2283422A1 (en) 1997-06-04 1998-12-10 Calgene, Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
US6566583B1 (en) 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US6013665A (en) 1997-12-16 2000-01-11 Abbott Laboratories Method for enhancing the absorption and transport of lipid soluble compounds using structured glycerides
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US7271315B2 (en) 1999-01-14 2007-09-18 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7217856B2 (en) 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
AU2001268296B2 (en) 2000-06-08 2006-05-25 Miami University Fatty acid elongase 3-ketoacyl coa synthase polypeptides
US20040010817A1 (en) 2000-07-21 2004-01-15 Washington State University Research Foundation Plant acyl-CoA synthetases
EP1911837B1 (en) 2000-09-28 2011-05-25 Bioriginal Food & Science Corp. FAD5-2 fatty acid desaturase family member and uses thereof
US6912470B2 (en) 2001-05-21 2005-06-28 Ecopia Biosciences, Inc. Genes and proteins involved in the biosynthesis of enediyne ring structures
TWI337619B (en) 2001-04-16 2011-02-21 Martek Biosciences Corp Pufa polyketide synthase systems and uses thereof
TWI377253B (en) 2001-04-16 2012-11-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US20040005672A1 (en) 2002-02-22 2004-01-08 Santi Daniel V. Heterologous production of polyketides
GB2385852A (en) 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
EP1487985B1 (en) 2002-03-16 2011-01-12 The University Of York Transgenic plants expressing enzymes involved in fatty acid biosynthesis
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
CN101389213B (zh) 2003-03-26 2014-05-28 Dsmip资产公司 Pufa聚酮化合物合酶系统及其用途
EP1613746B1 (de) 2003-03-31 2013-03-06 University Of Bristol Neue pflanzliche acyltransferasen spezifisch für langkettige, mehrfach ungesättigte fettsäuren
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7208590B2 (en) 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
US7700320B2 (en) 2004-02-13 2010-04-20 Martek Biosciences Corporation Schizochytrium fatty acid synthase (FAS) and products and methods related thereto
DE102004017370A1 (de) * 2004-04-08 2005-10-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh PUFA-PKS Gene aus Ulkenia
DE102004017369A1 (de) 2004-04-08 2005-11-03 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Screeningverfahren zur Identifizierung von PUFA-PKS in Proben
US20050255440A1 (en) * 2004-05-12 2005-11-17 Downing Linda P System and method of integrating levels of educational programs
DE102004060340A1 (de) 2004-07-16 2006-02-09 Basf Plant Science Gmbh Verfahren zur Erhöhung des Gehalts an mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
AR050881A1 (es) 2004-09-20 2006-11-29 Basf Plant Science Gmbh Genes de arabidopsis que codifican proteinas que participan en el metabolismo de azucares y lipidos y metodos de uso
US7355100B2 (en) 2004-09-30 2008-04-08 Dow Agrosciences Llc Canola cultivar NQC02CNX12
US7456340B2 (en) 2005-04-04 2008-11-25 Dow Agrosciences Llc Canola cultivar NQC02CNX21
US7348473B2 (en) 2004-09-30 2008-03-25 Dow Agrosciences Llc Canola cultivar NQC02CNX25
GB0421937D0 (en) 2004-10-02 2004-11-03 Univ York Acyl CoA synthetases
CA2613294A1 (en) 2005-05-17 2006-11-23 Photon Therapy Systems (Proprietary) Limited A photon therapy device
WO2006135866A2 (en) * 2005-06-10 2006-12-21 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
JPWO2007017952A1 (ja) 2005-08-11 2009-02-19 富士通株式会社 光学素子
JP2009530622A (ja) 2006-03-15 2009-08-27 クゥアルコム・インコーポレイテッド 全地球的航法衛星システム
AU2013251201A1 (en) 2006-03-15 2013-11-21 Dsm Ip Assets B.V. Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
WO2007106905A2 (en) 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
US8940884B2 (en) 2009-03-19 2015-01-27 Dsm Ip Assets B.V. Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, compositions, and methods of making and uses thereof
US9924733B2 (en) * 2010-01-19 2018-03-27 Dsm Ip Assets B.V. Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
TW201525136A (zh) * 2013-11-26 2015-07-01 Dow Agrosciences Llc 利用破囊壺菌ＰＵＦＡ合成酶於油籽作物中生成ω－３長鏈多不飽和脂肪酸

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050273883A1 (en) * 1999-01-14 2005-12-08 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US20080044871A1 (en) * 1999-01-14 2008-02-21 Martek Biosciences Corporation Pufa polyketide synthase systems and uses thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6013017410; Ｒ・Ｗ・オールド著: 遺伝子操作の原理 第３版, 1987, ｐ．１１５, 培風館 *
JPN7014002687; Ｓａｍｂｒｏｏｋ　＆　Ｒｕｓｓｅｌｌ: Ｍｏｌｅｃｕｌａｒ　Ｃｌｏｎｉｎｇ，　Ａ　Ｌａｂｏｒａｔｏｒｙ　Ｍａｎｕａｌ，３ｒｄ．　Ｅｄ Ｖｏｌ．２, 2001, １０．４７，　式（３） *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016537985A (ja) * 2013-11-26 2016-12-08 ダウ アグロサイエンシィズ エルエルシー スラウストキトリドｐｕｆａシンターゼによる油糧種子作物におけるオメガ−３長鎖多価不飽和脂肪酸の産生
JP2019514382A (ja) * 2016-05-12 2019-06-06 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． 微細藻類中でのオメガ−３多価不飽和脂肪酸産生を増加させる方法
US11987819B2 (en) 2016-05-12 2024-05-21 Dsm Ip Assets B.V. Method of increasing omega-3 polyunsaturated fatty acids production in microalgae

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