JP2012520933A5 - - Google Patents
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- Publication number
- JP2012520933A5 JP2012520933A5 JP2012500896A JP2012500896A JP2012520933A5 JP 2012520933 A5 JP2012520933 A5 JP 2012520933A5 JP 2012500896 A JP2012500896 A JP 2012500896A JP 2012500896 A JP2012500896 A JP 2012500896A JP 2012520933 A5 JP2012520933 A5 JP 2012520933A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- alkyl
- aryl
- amount
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 150000007523 nucleic acids Chemical class 0.000 claims 26
- 108020004707 nucleic acids Proteins 0.000 claims 24
- 102000039446 nucleic acids Human genes 0.000 claims 24
- 150000001875 compounds Chemical class 0.000 claims 16
- 238000000034 method Methods 0.000 claims 14
- 230000003321 amplification Effects 0.000 claims 10
- 125000003118 aryl group Chemical group 0.000 claims 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims 10
- 125000000217 alkyl group Chemical group 0.000 claims 9
- 125000004429 atom Chemical group 0.000 claims 7
- 125000000753 cycloalkyl group Chemical group 0.000 claims 6
- 125000005843 halogen group Chemical group 0.000 claims 6
- 125000001072 heteroaryl group Chemical group 0.000 claims 6
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 5
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 5
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 claims 5
- 239000011541 reaction mixture Substances 0.000 claims 5
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 claims 4
- 125000004209 (C1-C8) alkyl group Chemical group 0.000 claims 4
- 125000002877 alkyl aryl group Chemical group 0.000 claims 4
- -1 amino, hydroxy Chemical group 0.000 claims 4
- 125000005605 benzo group Chemical group 0.000 claims 4
- 239000000203 mixture Substances 0.000 claims 4
- 125000000094 2-phenylethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])([H])* 0.000 claims 3
- 125000006201 3-phenylpropyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims 3
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 claims 3
- 125000000623 heterocyclic group Chemical group 0.000 claims 3
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 2
- XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 238000001727 in vivo Methods 0.000 claims 1
- 230000001678 irradiating effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 238000005382 thermal cycling Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16056609P | 2009-03-16 | 2009-03-16 | |
| US61/160,566 | 2009-03-16 | ||
| PCT/US2010/027514 WO2010107816A2 (en) | 2009-03-16 | 2010-03-16 | Nucleic acid binding dyes and uses therefor |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012520933A JP2012520933A (ja) | 2012-09-10 |
| JP2012520933A5 true JP2012520933A5 (https=) | 2013-05-09 |
| JP5746135B2 JP5746135B2 (ja) | 2015-07-08 |
Family
ID=42731028
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012500896A Active JP5746135B2 (ja) | 2009-03-16 | 2010-03-16 | 核酸結合色素およびその使用 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US8598198B2 (https=) |
| EP (1) | EP2408863B1 (https=) |
| JP (1) | JP5746135B2 (https=) |
| WO (1) | WO2010107816A2 (https=) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2726588C (en) | 2008-06-03 | 2019-04-16 | Karl Kossen | Compounds and methods for treating inflammatory and fibrotic disorders |
| WO2010107816A2 (en) | 2009-03-16 | 2010-09-23 | Promega Corporation | Nucleic acid binding dyes and uses therefor |
| US8309059B2 (en) | 2009-08-31 | 2012-11-13 | Promega Corporation | Reactive cyanine compounds |
| US9493824B2 (en) | 2010-12-16 | 2016-11-15 | Bio-Rad Laboratories, Inc. | Universal reference dye for quantitative amplification |
| WO2014005125A2 (en) | 2012-06-29 | 2014-01-03 | Biotium, Inc. | Fluorescent compounds and uses thereof |
| US9034606B2 (en) * | 2012-08-23 | 2015-05-19 | New England Biolabs, Inc. | Detection of an amplification reaction product using pH-sensitive dyes |
| AR092742A1 (es) | 2012-10-02 | 2015-04-29 | Intermune Inc | Piridinonas antifibroticas |
| US10557853B2 (en) * | 2012-12-31 | 2020-02-11 | Yeda Research And Development Co. Ltd. | Molecular sensor and methods of use thereof |
| MX382781B (es) | 2014-04-02 | 2025-03-13 | Intermune Inc | Piridinonas anti-fibroticas. |
| BR112018068721A2 (pt) * | 2016-03-17 | 2019-01-22 | Delta Instr B V | método para contar células e aparelho |
| CN105753781B (zh) * | 2016-04-06 | 2018-01-26 | 陈科 | 一种4‑溴甲基喹啉酮的绿色合成方法 |
| US20180188204A1 (en) * | 2017-01-01 | 2018-07-05 | Sylvester Tumusiime | Use of charged quinine sulfate or other precursors or derivatives of quinine alkaloids in visualization of nucleic acids |
| WO2021105887A1 (en) | 2019-11-25 | 2021-06-03 | Emp Biotech Gmbh | Separation and isolation of nucleic acids using affinity ligands bound to a solid surface |
| JP7029841B1 (ja) * | 2020-08-21 | 2022-03-04 | 国立大学法人東北大学 | Ｒｎａ検出用蛍光色素及びその使用方法 |
| US12571031B2 (en) | 2020-09-02 | 2026-03-10 | Promega Corporation | Nucleic acid modifying reagents and uses thereof |
| JP2022132057A (ja) | 2021-02-26 | 2022-09-07 | 保土谷化学工業株式会社 | キサンテン色素、該色素を含有する着色組成物、カラーフィルター用着色剤およびカラーフィルター |
| JP2025507917A (ja) | 2022-03-01 | 2025-03-21 | プロメガ コーポレイション | 核酸架橋試薬及びその使用 |
| EP4602344A1 (en) | 2022-10-14 | 2025-08-20 | 10X Genomics, Inc. | Methods for analysis of biological samples |
| CN117164577B (zh) * | 2023-08-10 | 2024-11-15 | 郑州玛特瑞斯生物科技有限公司 | 一种dsDNA定量用荧光化合物、试剂盒、制备方法 |
| WO2025187095A1 (ja) * | 2024-03-04 | 2025-09-12 | 国立大学法人東北大学 | 化合物、蛍光色素、化合物の使用、試料中のｒｎａを検出するための方法、キット、エンベロープを有するウイルスに対する感染を検出するための方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE440738A (https=) | 1940-03-05 | |||
| US2269234A (en) | 1940-10-18 | 1942-01-06 | Eastman Kodak Co | Polymethine dye intermediates |
| US2398999A (en) * | 1942-09-01 | 1946-04-23 | Eastman Kodak Co | Polymethine dyes |
| US4665024A (en) | 1984-10-01 | 1987-05-12 | Becton, Dickinson And Company | Fluorescent gram stain |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| JPH05287266A (ja) * | 1992-04-09 | 1993-11-02 | Hitachi Chem Co Ltd | 蛍光標識用色素、蛍光標識用色素で標識された生物由来物質、及びそれらを含有する試薬 |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5534416A (en) * | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
| JP3918369B2 (ja) * | 1999-03-05 | 2007-05-23 | コニカミノルタホールディングス株式会社 | ハロゲン化銀乳剤、ハロゲン化銀感光材料、熱現像感光材料及びこれを用いた画像記録方法及び画像形成方法、並びに熱現像感光材料の製造方法 |
| WO2010107816A2 (en) | 2009-03-16 | 2010-09-23 | Promega Corporation | Nucleic acid binding dyes and uses therefor |
-
2010
- 2010-03-16 WO PCT/US2010/027514 patent/WO2010107816A2/en not_active Ceased
- 2010-03-16 JP JP2012500896A patent/JP5746135B2/ja active Active
- 2010-03-16 EP EP10709944.2A patent/EP2408863B1/en active Active
- 2010-03-16 US US12/725,251 patent/US8598198B2/en active Active
-
2013
- 2013-11-26 US US14/090,782 patent/US9206474B2/en active Active
-
2015
- 2015-12-07 US US14/960,589 patent/US9458499B2/en active Active
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