JP5746135B2 - 核酸結合色素およびその使用 - Google Patents

核酸結合色素およびその使用 Download PDF

Info

Publication number
JP5746135B2
JP5746135B2 JP2012500896A JP2012500896A JP5746135B2 JP 5746135 B2 JP5746135 B2 JP 5746135B2 JP 2012500896 A JP2012500896 A JP 2012500896A JP 2012500896 A JP2012500896 A JP 2012500896A JP 5746135 B2 JP5746135 B2 JP 5746135B2
Authority
JP
Japan
Prior art keywords
nucleic acid
alkyl
dye
fluorescence
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012500896A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012520933A (ja
JP2012520933A5 (https=
Inventor
マーク マクドゥーガル
マーク マクドゥーガル
スティーブン ドゥワイト
スティーブン ドゥワイト
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of JP2012520933A publication Critical patent/JP2012520933A/ja
Publication of JP2012520933A5 publication Critical patent/JP2012520933A5/ja
Application granted granted Critical
Publication of JP5746135B2 publication Critical patent/JP5746135B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
    • C07D513/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains two hetero rings
    • C07D513/06—Peri-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2012500896A 2009-03-16 2010-03-16 核酸結合色素およびその使用 Active JP5746135B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16056609P 2009-03-16 2009-03-16
US61/160,566 2009-03-16
PCT/US2010/027514 WO2010107816A2 (en) 2009-03-16 2010-03-16 Nucleic acid binding dyes and uses therefor

Publications (3)

Publication Number Publication Date
JP2012520933A JP2012520933A (ja) 2012-09-10
JP2012520933A5 JP2012520933A5 (https=) 2013-05-09
JP5746135B2 true JP5746135B2 (ja) 2015-07-08

Family

ID=42731028

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012500896A Active JP5746135B2 (ja) 2009-03-16 2010-03-16 核酸結合色素およびその使用

Country Status (4)

Country Link
US (3) US8598198B2 (https=)
EP (1) EP2408863B1 (https=)
JP (1) JP5746135B2 (https=)
WO (1) WO2010107816A2 (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2726588C (en) 2008-06-03 2019-04-16 Karl Kossen Compounds and methods for treating inflammatory and fibrotic disorders
WO2010107816A2 (en) 2009-03-16 2010-09-23 Promega Corporation Nucleic acid binding dyes and uses therefor
US8309059B2 (en) 2009-08-31 2012-11-13 Promega Corporation Reactive cyanine compounds
US9493824B2 (en) 2010-12-16 2016-11-15 Bio-Rad Laboratories, Inc. Universal reference dye for quantitative amplification
WO2014005125A2 (en) 2012-06-29 2014-01-03 Biotium, Inc. Fluorescent compounds and uses thereof
US9034606B2 (en) * 2012-08-23 2015-05-19 New England Biolabs, Inc. Detection of an amplification reaction product using pH-sensitive dyes
AR092742A1 (es) 2012-10-02 2015-04-29 Intermune Inc Piridinonas antifibroticas
US10557853B2 (en) * 2012-12-31 2020-02-11 Yeda Research And Development Co. Ltd. Molecular sensor and methods of use thereof
MX382781B (es) 2014-04-02 2025-03-13 Intermune Inc Piridinonas anti-fibroticas.
BR112018068721A2 (pt) * 2016-03-17 2019-01-22 Delta Instr B V método para contar células e aparelho
CN105753781B (zh) * 2016-04-06 2018-01-26 陈科 一种4‑溴甲基喹啉酮的绿色合成方法
US20180188204A1 (en) * 2017-01-01 2018-07-05 Sylvester Tumusiime Use of charged quinine sulfate or other precursors or derivatives of quinine alkaloids in visualization of nucleic acids
WO2021105887A1 (en) 2019-11-25 2021-06-03 Emp Biotech Gmbh Separation and isolation of nucleic acids using affinity ligands bound to a solid surface
JP7029841B1 (ja) * 2020-08-21 2022-03-04 国立大学法人東北大学 Ｒｎａ検出用蛍光色素及びその使用方法
US12571031B2 (en) 2020-09-02 2026-03-10 Promega Corporation Nucleic acid modifying reagents and uses thereof
JP2022132057A (ja) 2021-02-26 2022-09-07 保土谷化学工業株式会社 キサンテン色素、該色素を含有する着色組成物、カラーフィルター用着色剤およびカラーフィルター
JP2025507917A (ja) 2022-03-01 2025-03-21 プロメガ コーポレイション 核酸架橋試薬及びその使用
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
CN117164577B (zh) * 2023-08-10 2024-11-15 郑州玛特瑞斯生物科技有限公司 一种dsDNA定量用荧光化合物、试剂盒、制备方法
WO2025187095A1 (ja) * 2024-03-04 2025-09-12 国立大学法人東北大学 化合物、蛍光色素、化合物の使用、試料中のｒｎａを検出するための方法、キット、エンベロープを有するウイルスに対する感染を検出するための方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE440738A (https=) 1940-03-05
US2269234A (en) 1940-10-18 1942-01-06 Eastman Kodak Co Polymethine dye intermediates
US2398999A (en) * 1942-09-01 1946-04-23 Eastman Kodak Co Polymethine dyes
US4665024A (en) 1984-10-01 1987-05-12 Becton, Dickinson And Company Fluorescent gram stain
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
JPH05287266A (ja) * 1992-04-09 1993-11-02 Hitachi Chem Co Ltd 蛍光標識用色素、蛍光標識用色素で標識された生物由来物質、及びそれらを含有する試薬
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5534416A (en) * 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
JP3918369B2 (ja) * 1999-03-05 2007-05-23 コニカミノルタホールディングス株式会社 ハロゲン化銀乳剤、ハロゲン化銀感光材料、熱現像感光材料及びこれを用いた画像記録方法及び画像形成方法、並びに熱現像感光材料の製造方法
WO2010107816A2 (en) 2009-03-16 2010-09-23 Promega Corporation Nucleic acid binding dyes and uses therefor

Also Published As

Publication number Publication date
WO2010107816A2 (en) 2010-09-23
US9206474B2 (en) 2015-12-08
EP2408863A2 (en) 2012-01-25
US8598198B2 (en) 2013-12-03
JP2012520933A (ja) 2012-09-10
WO2010107816A3 (en) 2010-12-16
US20140178882A1 (en) 2014-06-26
US20100233710A1 (en) 2010-09-16
US9458499B2 (en) 2016-10-04
US20160083782A1 (en) 2016-03-24
EP2408863B1 (en) 2018-01-10

Similar Documents

Publication Publication Date Title
JP5746135B2 (ja) 核酸結合色素およびその使用
JP7227741B2 (ja) カルボキシｘローダミン類似体
JP4503047B2 (ja) 選択された透過性を有する置換非対称シアニン色素
US9139868B2 (en) Silicon and germanium dyes for use in genetic identity
CN106147752B (zh) 一种rna荧光探针及其制备方法和应用
JP7029841B1 (ja) Ｒｎａ検出用蛍光色素及びその使用方法
US8742114B2 (en) Nucleic acid detections and methods of their use
JP5730311B2 (ja) 反応性シアニン化合物
US20200263231A1 (en) Fluorogenic dyes for high sensitivity DNA detection

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130318

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130318

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140714

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141014

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150422

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150507

R150 Certificate of patent or registration of utility model

Ref document number: 5746135

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250