JP2012500634A - 鳥類から得られた抗体のクローニング方法 - Google Patents

鳥類から得られた抗体のクローニング方法 Download PDF

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Publication number
JP2012500634A
JP2012500634A JP2011524188A JP2011524188A JP2012500634A JP 2012500634 A JP2012500634 A JP 2012500634A JP 2011524188 A JP2011524188 A JP 2011524188A JP 2011524188 A JP2011524188 A JP 2011524188A JP 2012500634 A JP2012500634 A JP 2012500634A
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cells
igy
expression
multiplex
amplification
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JP2012500634A5 (https=
Inventor
ラース・セゴー・ニールセン
アラン・イェンセン
チャールズ・パイク
アンネ・マリー・ヴァレンティン・イェンセン
クラウス・ケーフェーズ
メッテ・トルン
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Symphogen AS
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Symphogen AS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2875—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF/TNF superfamily, e.g. CD70, CD95L, CD153, CD154
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Environmental Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2011524188A 2008-08-29 2009-08-28 鳥類から得られた抗体のクローニング方法 Pending JP2012500634A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200801190 2008-08-29
DKPA200801190 2008-08-29
US13639008P 2008-09-02 2008-09-02
US61/136,390 2008-09-02
PCT/DK2009/050219 WO2010022738A1 (en) 2008-08-29 2009-08-28 Method for cloning avian-derived antibodies

Publications (2)

Publication Number Publication Date
JP2012500634A true JP2012500634A (ja) 2012-01-12
JP2012500634A5 JP2012500634A5 (https=) 2012-06-07

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ID=40637072

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JP2011524188A Pending JP2012500634A (ja) 2008-08-29 2009-08-28 鳥類から得られた抗体のクローニング方法

Country Status (15)

Country Link
US (1) US20100069262A1 (https=)
EP (1) EP2331689A4 (https=)
JP (1) JP2012500634A (https=)
KR (1) KR20110058861A (https=)
CN (1) CN102137928A (https=)
AU (1) AU2009287165A1 (https=)
BR (1) BRPI0917352A2 (https=)
CA (1) CA2735020A1 (https=)
IL (1) IL210481A0 (https=)
MX (1) MX2011001930A (https=)
NZ (1) NZ590562A (https=)
RU (1) RU2011111725A (https=)
TW (1) TW201014908A (https=)
WO (1) WO2010022738A1 (https=)
ZA (1) ZA201100304B (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016533769A (ja) * 2013-10-07 2016-11-04 プレステージ バイオファーマ プライベート リミテッド 抗体発現用バイシストロニック発現ベクター及びそれを用いた抗体の生産方法
JP2020511117A (ja) * 2016-12-23 2020-04-16 ビステラ， インコーポレイテッド 結合ポリペプチドおよびそれを作製する方法

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DK2121920T3 (da) * 2007-03-01 2011-11-21 Symphogen As Fremgangsmåde til kloning af sammenhørende antistoffer
JP2008289483A (ja) * 2007-05-25 2008-12-04 Symphogen As 真核生物系において発現可能な形質転換体のスクリーニング
EP2750496A4 (en) * 2011-09-01 2015-06-10 Synageva Biopharma Corp CHIMÄRE HUMANE IMMUNGLOBULINE PRODUCING TRANSGENE BIRDS
EP3597038B1 (en) * 2012-02-01 2021-04-21 Regeneron Pharmaceuticals, Inc. Humanized rodents that express heavy chains containing vl domains
CN104231071B (zh) * 2014-07-04 2017-10-24 东北农业大学 鹅CD3ε链胞外区单克隆抗体及其在检测鹅CD3+T淋巴细胞中的应用
ES2924402T3 (es) 2015-10-02 2022-10-06 Symphogen As Anticuerpos anti-PD-1 y composiciones
JP7443376B2 (ja) * 2018-12-31 2024-03-05 メルス ナムローゼ フェンノートシャップ 混合結合ドメイン
CN115109794A (zh) * 2022-06-30 2022-09-27 武汉生之源生物科技股份有限公司 一种噬菌粒载体及其制备方法和应用
CN116987195B (zh) * 2023-09-28 2023-12-15 北京索莱宝科技有限公司 小鼠抗鸭IgY单克隆抗体、细胞株及其应用

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JP2001519824A (ja) * 1997-04-14 2001-10-23 ミクロメート・アクチエンゲゼルシャフト 抗ヒト抗原受容体を産生するための斬新な方法およびそれらの使用
WO2001088162A2 (en) * 2000-05-16 2001-11-22 Genway Biotech, Inc. Methods and vectors for generating antibodies in avian species and uses therefor
JP2005506959A (ja) * 2001-06-13 2005-03-10 オステオペップ−ファーマ ゲゼルシャフト ミット ベシュレンクテル ハフツング 腫瘍およびその転移の治療のための医薬
JP2007505611A (ja) * 2003-09-18 2007-03-15 シムフォゲン・アクティーゼルスカブ 目的とする配列を連結するための方法
US20070141049A1 (en) * 2005-08-26 2007-06-21 Reinhard Bredehorst Bivalent IgY antibody constructs for diagnostic and therapeutic applications
WO2007124755A1 (en) * 2006-05-02 2007-11-08 The Antibody Project Aps Method for immunizing an avian species

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WO2001088162A2 (en) * 2000-05-16 2001-11-22 Genway Biotech, Inc. Methods and vectors for generating antibodies in avian species and uses therefor
JP2005506959A (ja) * 2001-06-13 2005-03-10 オステオペップ−ファーマ ゲゼルシャフト ミット ベシュレンクテル ハフツング 腫瘍およびその転移の治療のための医薬
JP2007505611A (ja) * 2003-09-18 2007-03-15 シムフォゲン・アクティーゼルスカブ 目的とする配列を連結するための方法
US20070141049A1 (en) * 2005-08-26 2007-06-21 Reinhard Bredehorst Bivalent IgY antibody constructs for diagnostic and therapeutic applications
WO2007124755A1 (en) * 2006-05-02 2007-11-08 The Antibody Project Aps Method for immunizing an avian species

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016533769A (ja) * 2013-10-07 2016-11-04 プレステージ バイオファーマ プライベート リミテッド 抗体発現用バイシストロニック発現ベクター及びそれを用いた抗体の生産方法
US11046975B2 (en) 2013-10-07 2021-06-29 Prestige Biopharma Pte. Ltd. Bicistronic expression vector for antibody expression and method for producing antibody using same
JP2020511117A (ja) * 2016-12-23 2020-04-16 ビステラ， インコーポレイテッド 結合ポリペプチドおよびそれを作製する方法
JP7360324B2 (ja) 2016-12-23 2023-10-12 ビステラ， インコーポレイテッド 結合ポリペプチドおよびそれを作製する方法
JP2023169422A (ja) * 2016-12-23 2023-11-29 ビステラ， インコーポレイテッド 結合ポリペプチドおよびそれを作製する方法

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EP2331689A1 (en) 2011-06-15
EP2331689A4 (en) 2012-10-31
CA2735020A1 (en) 2010-03-04
WO2010022738A1 (en) 2010-03-04
NZ590562A (en) 2012-08-31
RU2011111725A (ru) 2012-10-10
AU2009287165A1 (en) 2010-03-04
US20100069262A1 (en) 2010-03-18
ZA201100304B (en) 2011-10-26
CN102137928A (zh) 2011-07-27
TW201014908A (en) 2010-04-16
KR20110058861A (ko) 2011-06-01
IL210481A0 (en) 2011-03-31
BRPI0917352A2 (pt) 2017-08-22
MX2011001930A (es) 2011-04-21

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