CA2735020A1 - Method for cloning avian-derived antibodies - Google Patents

Method for cloning avian-derived antibodies Download PDF

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Publication number
CA2735020A1
CA2735020A1 CA2735020A CA2735020A CA2735020A1 CA 2735020 A1 CA2735020 A1 CA 2735020A1 CA 2735020 A CA2735020 A CA 2735020A CA 2735020 A CA2735020 A CA 2735020A CA 2735020 A1 CA2735020 A1 CA 2735020A1
Authority
CA
Canada
Prior art keywords
cells
igy
multiplex
sequences
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2735020A
Other languages
English (en)
French (fr)
Inventor
Lars Soegaard Nielsen
Allan Jensen
Charles Pyke
Anne Marie Valentin Jensen
Klaus Koefoed
Mette Thorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Symphogen AS
Original Assignee
Symphogen AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen AS filed Critical Symphogen AS
Publication of CA2735020A1 publication Critical patent/CA2735020A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2875—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF/TNF superfamily, e.g. CD70, CD95L, CD153, CD154
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Environmental Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2735020A 2008-08-29 2009-08-28 Method for cloning avian-derived antibodies Abandoned CA2735020A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200801190 2008-08-29
DKPA200801190 2008-08-29
US13639008P 2008-09-02 2008-09-02
US61/136,390 2008-09-02
PCT/DK2009/050219 WO2010022738A1 (en) 2008-08-29 2009-08-28 Method for cloning avian-derived antibodies

Publications (1)

Publication Number Publication Date
CA2735020A1 true CA2735020A1 (en) 2010-03-04

Family

ID=40637072

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2735020A Abandoned CA2735020A1 (en) 2008-08-29 2009-08-28 Method for cloning avian-derived antibodies

Country Status (15)

Country Link
US (1) US20100069262A1 (https=)
EP (1) EP2331689A4 (https=)
JP (1) JP2012500634A (https=)
KR (1) KR20110058861A (https=)
CN (1) CN102137928A (https=)
AU (1) AU2009287165A1 (https=)
BR (1) BRPI0917352A2 (https=)
CA (1) CA2735020A1 (https=)
IL (1) IL210481A0 (https=)
MX (1) MX2011001930A (https=)
NZ (1) NZ590562A (https=)
RU (1) RU2011111725A (https=)
TW (1) TW201014908A (https=)
WO (1) WO2010022738A1 (https=)
ZA (1) ZA201100304B (https=)

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JP2008289483A (ja) * 2007-05-25 2008-12-04 Symphogen As 真核生物系において発現可能な形質転換体のスクリーニング
EP2750496A4 (en) * 2011-09-01 2015-06-10 Synageva Biopharma Corp CHIMÄRE HUMANE IMMUNGLOBULINE PRODUCING TRANSGENE BIRDS
EP3597038B1 (en) * 2012-02-01 2021-04-21 Regeneron Pharmaceuticals, Inc. Humanized rodents that express heavy chains containing vl domains
EP3683314A1 (en) 2013-10-07 2020-07-22 Prestige Biopharma Pte. Ltd. Bicistronic expression vector for antibody expression and method for producing antibody using same
CN104231071B (zh) * 2014-07-04 2017-10-24 东北农业大学 鹅CD3ε链胞外区单克隆抗体及其在检测鹅CD3+T淋巴细胞中的应用
ES2924402T3 (es) 2015-10-02 2022-10-06 Symphogen As Anticuerpos anti-PD-1 y composiciones
KR102702552B1 (ko) * 2016-12-23 2024-09-03 비스테라, 인크. 결합 폴리펩타이드 및 이의 제조 방법
JP7443376B2 (ja) * 2018-12-31 2024-03-05 メルス ナムローゼ フェンノートシャップ 混合結合ドメイン
CN115109794A (zh) * 2022-06-30 2022-09-27 武汉生之源生物科技股份有限公司 一种噬菌粒载体及其制备方法和应用
CN116987195B (zh) * 2023-09-28 2023-12-15 北京索莱宝科技有限公司 小鼠抗鸭IgY单克隆抗体、细胞株及其应用

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US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US5906936A (en) * 1988-05-04 1999-05-25 Yeda Research And Development Co. Ltd. Endowing lymphocytes with antibody specificity
US6284471B1 (en) * 1991-03-18 2001-09-04 New York University Medical Center Anti-TNFa antibodies and assays employing anti-TNFa antibodies
US6277969B1 (en) * 1991-03-18 2001-08-21 New York University Anti-TNF antibodies and peptides of human tumor necrosis factor
US6096878A (en) * 1993-05-10 2000-08-01 Japan Tobacco Inc. Human immunoglobulin VH gene segments and DNA fragments containing the same
US6111166A (en) * 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
US6258529B1 (en) * 1994-12-01 2001-07-10 Oravax, Inc. PCR amplification of rearranged genomic variable regions of immunoglobulin genes
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WO2001088162A2 (en) * 2000-05-16 2001-11-22 Genway Biotech, Inc. Methods and vectors for generating antibodies in avian species and uses therefor
US6849259B2 (en) * 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
US20020028488A1 (en) * 2000-06-19 2002-03-07 Sujay Singh Transgenic avian species for making human and chimeric antibodies
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JP2008289483A (ja) * 2007-05-25 2008-12-04 Symphogen As 真核生物系において発現可能な形質転換体のスクリーニング
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Also Published As

Publication number Publication date
EP2331689A1 (en) 2011-06-15
EP2331689A4 (en) 2012-10-31
WO2010022738A1 (en) 2010-03-04
NZ590562A (en) 2012-08-31
RU2011111725A (ru) 2012-10-10
AU2009287165A1 (en) 2010-03-04
US20100069262A1 (en) 2010-03-18
ZA201100304B (en) 2011-10-26
CN102137928A (zh) 2011-07-27
TW201014908A (en) 2010-04-16
KR20110058861A (ko) 2011-06-01
JP2012500634A (ja) 2012-01-12
IL210481A0 (en) 2011-03-31
BRPI0917352A2 (pt) 2017-08-22
MX2011001930A (es) 2011-04-21

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20140828