KR20110058861A - 조류 유래 항체의 클로닝 방법 - Google Patents

조류 유래 항체의 클로닝 방법 Download PDF

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Publication number
KR20110058861A
KR20110058861A KR1020117007231A KR20117007231A KR20110058861A KR 20110058861 A KR20110058861 A KR 20110058861A KR 1020117007231 A KR1020117007231 A KR 1020117007231A KR 20117007231 A KR20117007231 A KR 20117007231A KR 20110058861 A KR20110058861 A KR 20110058861A
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KR
South Korea
Prior art keywords
igy
cells
multiplex
variable region
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
KR1020117007231A
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English (en)
Korean (ko)
Inventor
라르스 소에가아르드 닐센
알란 젠센
찰스 피케
안네 마리에 발렌틴 젠센
클라우스 코에포에드
메테 쏜
Original Assignee
심포젠 에이/에스
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Publication date
Application filed by 심포젠 에이/에스 filed Critical 심포젠 에이/에스
Publication of KR20110058861A publication Critical patent/KR20110058861A/ko
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2875—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF/TNF superfamily, e.g. CD70, CD95L, CD153, CD154
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Environmental Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020117007231A 2008-08-29 2009-08-28 조류 유래 항체의 클로닝 방법 Withdrawn KR20110058861A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200801190 2008-08-29
DKPA200801190 2008-08-29
US13639008P 2008-09-02 2008-09-02
US61/136,390 2008-09-02

Publications (1)

Publication Number Publication Date
KR20110058861A true KR20110058861A (ko) 2011-06-01

Family

ID=40637072

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020117007231A Withdrawn KR20110058861A (ko) 2008-08-29 2009-08-28 조류 유래 항체의 클로닝 방법

Country Status (15)

Country Link
US (1) US20100069262A1 (https=)
EP (1) EP2331689A4 (https=)
JP (1) JP2012500634A (https=)
KR (1) KR20110058861A (https=)
CN (1) CN102137928A (https=)
AU (1) AU2009287165A1 (https=)
BR (1) BRPI0917352A2 (https=)
CA (1) CA2735020A1 (https=)
IL (1) IL210481A0 (https=)
MX (1) MX2011001930A (https=)
NZ (1) NZ590562A (https=)
RU (1) RU2011111725A (https=)
TW (1) TW201014908A (https=)
WO (1) WO2010022738A1 (https=)
ZA (1) ZA201100304B (https=)

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JP2008289483A (ja) * 2007-05-25 2008-12-04 Symphogen As 真核生物系において発現可能な形質転換体のスクリーニング
EP2750496A4 (en) * 2011-09-01 2015-06-10 Synageva Biopharma Corp CHIMÄRE HUMANE IMMUNGLOBULINE PRODUCING TRANSGENE BIRDS
EP3597038B1 (en) * 2012-02-01 2021-04-21 Regeneron Pharmaceuticals, Inc. Humanized rodents that express heavy chains containing vl domains
EP3683314A1 (en) 2013-10-07 2020-07-22 Prestige Biopharma Pte. Ltd. Bicistronic expression vector for antibody expression and method for producing antibody using same
CN104231071B (zh) * 2014-07-04 2017-10-24 东北农业大学 鹅CD3ε链胞外区单克隆抗体及其在检测鹅CD3+T淋巴细胞中的应用
ES2924402T3 (es) 2015-10-02 2022-10-06 Symphogen As Anticuerpos anti-PD-1 y composiciones
KR102702552B1 (ko) * 2016-12-23 2024-09-03 비스테라, 인크. 결합 폴리펩타이드 및 이의 제조 방법
JP7443376B2 (ja) * 2018-12-31 2024-03-05 メルス ナムローゼ フェンノートシャップ 混合結合ドメイン
CN115109794A (zh) * 2022-06-30 2022-09-27 武汉生之源生物科技股份有限公司 一种噬菌粒载体及其制备方法和应用
CN116987195B (zh) * 2023-09-28 2023-12-15 北京索莱宝科技有限公司 小鼠抗鸭IgY单克隆抗体、细胞株及其应用

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US5576195A (en) * 1985-11-01 1996-11-19 Xoma Corporation Vectors with pectate lyase signal sequence
US5906936A (en) * 1988-05-04 1999-05-25 Yeda Research And Development Co. Ltd. Endowing lymphocytes with antibody specificity
US6284471B1 (en) * 1991-03-18 2001-09-04 New York University Medical Center Anti-TNFa antibodies and assays employing anti-TNFa antibodies
US6277969B1 (en) * 1991-03-18 2001-08-21 New York University Anti-TNF antibodies and peptides of human tumor necrosis factor
US6096878A (en) * 1993-05-10 2000-08-01 Japan Tobacco Inc. Human immunoglobulin VH gene segments and DNA fragments containing the same
US6111166A (en) * 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
US6258529B1 (en) * 1994-12-01 2001-07-10 Oravax, Inc. PCR amplification of rearranged genomic variable regions of immunoglobulin genes
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
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US6194551B1 (en) * 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
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WO2001088162A2 (en) * 2000-05-16 2001-11-22 Genway Biotech, Inc. Methods and vectors for generating antibodies in avian species and uses therefor
US6849259B2 (en) * 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
US20020028488A1 (en) * 2000-06-19 2002-03-07 Sujay Singh Transgenic avian species for making human and chimeric antibodies
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Also Published As

Publication number Publication date
EP2331689A1 (en) 2011-06-15
EP2331689A4 (en) 2012-10-31
CA2735020A1 (en) 2010-03-04
WO2010022738A1 (en) 2010-03-04
NZ590562A (en) 2012-08-31
RU2011111725A (ru) 2012-10-10
AU2009287165A1 (en) 2010-03-04
US20100069262A1 (en) 2010-03-18
ZA201100304B (en) 2011-10-26
CN102137928A (zh) 2011-07-27
TW201014908A (en) 2010-04-16
JP2012500634A (ja) 2012-01-12
IL210481A0 (en) 2011-03-31
BRPI0917352A2 (pt) 2017-08-22
MX2011001930A (es) 2011-04-21

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Date Code Title Description
PA0105 International application

Patent event date: 20110329

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid