JP2011514153A5 - - Google Patents

Info

Publication number

JP2011514153A5

JP2011514153A5 JP2010547110A JP2010547110A JP2011514153A5 JP 2011514153 A5 JP2011514153 A5 JP 2011514153A5 JP 2010547110 A JP2010547110 A JP 2010547110A JP 2010547110 A JP2010547110 A JP 2010547110A JP 2011514153 A5 JP2011514153 A5 JP 2011514153A5

Authority

JP

Japan

Prior art keywords

enzyme

dehydrogenase

stabilized

independently

stable coenzyme

Prior art date

2009-02-19

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 238000000034 method Methods 0.000 claims 24
• 108090000790 Enzymes Proteins 0.000 claims 18
• 102000004190 Enzymes Human genes 0.000 claims 18
• 239000005515 coenzyme Substances 0.000 claims 12
• 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims 6
• 229910052717 sulfur Inorganic materials 0.000 claims 5
• 125000004122 cyclic group Chemical group 0.000 claims 4
• 229910052760 oxygen Inorganic materials 0.000 claims 4
• 101710088194 Dehydrogenase Proteins 0.000 claims 3
• BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims 3
• XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 claims 3
• 150000001875 compounds Chemical class 0.000 claims 3
• 229910052739 hydrogen Inorganic materials 0.000 claims 3
• 229950006238 nadide Drugs 0.000 claims 3
• 108010021809 Alcohol dehydrogenase Proteins 0.000 claims 2
• 102000007698 Alcohol dehydrogenase Human genes 0.000 claims 2
• 108030000198 L-amino-acid dehydrogenases Proteins 0.000 claims 2
• 125000004429 atom Chemical group 0.000 claims 2
• 239000003153 chemical reaction reagent Substances 0.000 claims 2
• 239000002274 desiccant Substances 0.000 claims 2
• 238000001514 detection method Methods 0.000 claims 2
• 125000005842 heteroatom Chemical group 0.000 claims 2
• 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims 2
• 229910052757 nitrogen Inorganic materials 0.000 claims 2
• 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 2
• BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims 1
• GVNVAWHJIKLAGL-UHFFFAOYSA-N 2-(cyclohexen-1-yl)cyclohexan-1-one Chemical compound O=C1CCCCC1C1=CCCCC1 GVNVAWHJIKLAGL-UHFFFAOYSA-N 0.000 claims 1
• 108010050201 2-hydroxybutyrate dehydrogenase Proteins 0.000 claims 1
• 229930024421 Adenine Natural products 0.000 claims 1
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
• 101150065749 Churc1 gene Proteins 0.000 claims 1
• 108020005199 Dehydrogenases Proteins 0.000 claims 1
• 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 claims 1
• 108700040097 Glycerol dehydrogenases Proteins 0.000 claims 1
• 108010009384 L-Iditol 2-Dehydrogenase Proteins 0.000 claims 1
• 102000003855 L-lactate dehydrogenase Human genes 0.000 claims 1
• 108700023483 L-lactate dehydrogenases Proteins 0.000 claims 1
• 102100038239 Protein Churchill Human genes 0.000 claims 1
• JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical group C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims 1
• 102100026974 Sorbitol dehydrogenase Human genes 0.000 claims 1
• 229960000643 adenine Drugs 0.000 claims 1
• BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims 1
• 150000001413 amino acids Chemical class 0.000 claims 1
• 239000012491 analyte Substances 0.000 claims 1
• 229910052799 carbon Inorganic materials 0.000 claims 1
• 125000004432 carbon atom Chemical group C* 0.000 claims 1
• 125000006297 carbonyl amino group Chemical group [H]N([*:2])C([*:1])=O 0.000 claims 1
• 230000000694 effects Effects 0.000 claims 1
• 230000003301 hydrolyzing effect Effects 0.000 claims 1
• 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 1
• 239000007791 liquid phase Substances 0.000 claims 1
• 235000011090 malic acid Nutrition 0.000 claims 1
• 239000001630 malic acid Substances 0.000 claims 1
• BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims 1
• 125000000962 organic group Chemical group 0.000 claims 1
• 150000003839 salts Chemical class 0.000 claims 1
• 230000000087 stabilizing effect Effects 0.000 claims 1
• 125000001424 substituent group Chemical group 0.000 claims 1