ZA201006638B - Stabilization of dehydrogenases using stable coezymes - Google Patents

Stabilization of dehydrogenases using stable coezymes

Info

Publication number
ZA201006638B
ZA201006638B ZA2010/06638A ZA201006638A ZA201006638B ZA 201006638 B ZA201006638 B ZA 201006638B ZA 2010/06638 A ZA2010/06638 A ZA 2010/06638A ZA 201006638 A ZA201006638 A ZA 201006638A ZA 201006638 B ZA201006638 B ZA 201006638B
Authority
ZA
South Africa
Prior art keywords
coezymes
dehydrogenases
stabilization
stable
stable coezymes
Prior art date
Application number
ZA2010/06638A
Other languages
English (en)
Inventor
Joachim Hoenes
Claudia Gaessler-Dietsche
Carina Horn
Dieter Heindl
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of ZA201006638B publication Critical patent/ZA201006638B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01005—L-Amino-acid dehydrogenase (1.4.1.5)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
ZA2010/06638A 2008-02-19 2010-09-16 Stabilization of dehydrogenases using stable coezymes ZA201006638B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08003054A EP2093284A1 (de) 2008-02-19 2008-02-19 Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
PCT/EP2009/001206 WO2009103540A1 (de) 2008-02-19 2009-02-19 Stabilisierung von dehydrogenasen mit stabilen coenzymen

Publications (1)

Publication Number Publication Date
ZA201006638B true ZA201006638B (en) 2011-06-29

Family

ID=39768713

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2010/06638A ZA201006638B (en) 2008-02-19 2010-09-16 Stabilization of dehydrogenases using stable coezymes

Country Status (15)

Country Link
US (2) US9896666B2 (cg-RX-API-DMAC7.html)
EP (2) EP2093284A1 (cg-RX-API-DMAC7.html)
JP (2) JP5758128B2 (cg-RX-API-DMAC7.html)
KR (3) KR101381004B1 (cg-RX-API-DMAC7.html)
CN (3) CN104450659A (cg-RX-API-DMAC7.html)
AR (1) AR070436A1 (cg-RX-API-DMAC7.html)
AU (1) AU2009216908B9 (cg-RX-API-DMAC7.html)
BR (1) BRPI0907839A2 (cg-RX-API-DMAC7.html)
CA (1) CA2721718C (cg-RX-API-DMAC7.html)
HK (1) HK1208703A1 (cg-RX-API-DMAC7.html)
MX (1) MX2010008920A (cg-RX-API-DMAC7.html)
RU (1) RU2499834C2 (cg-RX-API-DMAC7.html)
TW (1) TWI482860B (cg-RX-API-DMAC7.html)
WO (1) WO2009103540A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA201006638B (cg-RX-API-DMAC7.html)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7553615B2 (en) * 2005-07-28 2009-06-30 Roche Diagnostics Operations, Inc. Compounds, methods, complexes, apparatuses and uses relating to stabile forms of NAD/NADH
EP2093284A1 (de) 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
WO2010094632A1 (de) * 2009-02-19 2010-08-26 F. Hoffmann-La Roche Ag Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
EP2226007A1 (de) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Testelementmagazin mit abgedeckten Testfeldern
EP2226008A1 (de) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Verfahren zur Herstellung eines analytischen Magazins
EP2398909B1 (de) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
EP2398388B1 (de) 2009-02-19 2020-04-08 Roche Diabetes Care GmbH Platzsparende magazinierung analytischer hilfsmittel
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
MX2012006706A (es) * 2009-12-16 2012-07-03 Hoffmann La Roche Deteccion de la descomposicion de las enzimas en un elemento de prueba mediante la liberacion controlada de analito protegido.
EP2636750A1 (en) * 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
PL2864765T3 (pl) 2012-06-22 2021-10-11 F.Hoffmann-La Roche Ag Sposób i urządzenie do wykrywania analitu w płynie ustrojowym
US8921061B2 (en) * 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Reagent materials and associated test elements
US8920628B2 (en) 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Systems and methods for multiple analyte analysis
CA2884919C (en) 2012-12-20 2021-05-04 F. Hoffmann-La Roche Ag Method for analyzing a sample of a body fluid
HUE034313T2 (en) 2012-12-20 2018-02-28 Hoffmann La Roche Procedures for evaluating medical measurement curves
EP2781919A1 (en) 2013-03-19 2014-09-24 Roche Diagniostics GmbH Method / device for generating a corrected value of an analyte concentration in a sample of a body fluid
EP2994536B1 (en) 2013-05-08 2017-06-14 Roche Diabetes Care GmbH Stabilization of enzymes by nicotinic acid
CN105247069A (zh) * 2013-06-04 2016-01-13 豪夫迈·罗氏有限公司 用于fret的新化合物及与其相关的方法
WO2015063025A1 (en) 2013-10-29 2015-05-07 F. Hoffmann-La Roche Ag Nano-enzyme containers for test elements
WO2015078899A1 (en) 2013-11-27 2015-06-04 Roche Diagnostics Gmbh Composition comprising up-converting phosphors for detecting an analyte
EP2927319A1 (en) 2014-03-31 2015-10-07 Roche Diagnostics GmbH High load enzyme immobilization by crosslinking
WO2015158645A1 (en) 2014-04-14 2015-10-22 Roche Diagnostics Gmbh Phenazinium mediators
EP3757096B1 (en) 2014-08-22 2025-03-12 Roche Diagnostics GmbH Redoxindicators for a use in cell viability testing
CN107779459B (zh) * 2016-08-31 2021-06-08 安琪酵母股份有限公司 葡萄糖脱氢酶dna分子、载体和菌株及应用
EP4481375A3 (en) 2016-10-05 2025-04-02 F. Hoffmann-La Roche AG Detection reagents and electrode arrangements for multi-analyte diagnostic test elements, as well as methods of using the same
EP3339431A1 (en) 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
EP3759231B1 (en) 2018-02-28 2023-10-18 F. Hoffmann-La Roche AG Biocompatibility coating for continuous analyte measurement

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1043568A1 (ru) * 1981-06-11 1983-09-23 Московский Ордена Ленина,Ордена Октябрьской Революции И Ордена Трудового Красного Знамени Государственный Университет Им.М.В.Ломоносова Способ определени активности дегидрогеназ крови
DE19543493A1 (de) * 1995-11-22 1997-05-28 Boehringer Mannheim Gmbh Stabilisierte Coenzym-Lösungen und deren Verwendung zur Bestimmung von Dehydrogenasen bzw. deren Substrate im alkalischen Milieu
US5801006A (en) 1997-02-04 1998-09-01 Specialty Assays, Inc. Use of NADPH and NADH analogs in the measurement of enzyme activities and metabolites
US6380380B1 (en) 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
WO2001094370A1 (en) 2000-06-07 2001-12-13 Wako Pure Chemical Industries, Ltd. Coenzyme derivatives and enzymes appropriate therefor
WO2002036779A1 (fr) 2000-10-31 2002-05-10 Koji Sode Nouvelle glucose deshydrogenase et procede de production de la deshydrogenase
JP2003310274A (ja) * 2002-04-30 2003-11-05 Amano Enzyme Inc グルコース脱水素酵素およびそれをコードする遺伝子
US8846132B2 (en) 2002-05-16 2014-09-30 Roche Diagnostics Operations, Inc. Method for producing polymer layers
CA2535147A1 (en) * 2003-08-11 2005-05-19 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides
ES2572775T3 (es) 2004-03-06 2016-06-02 F. Hoffmann-La Roche Ag Dispositivo para la extracción de líquidos corporales
US7172890B2 (en) * 2004-10-28 2007-02-06 Roche Diagnostics Gmbh Inactivated enzyme variants and associated process and reagent system
DE102005035461A1 (de) * 2005-07-28 2007-02-15 Roche Diagnostics Gmbh Stabile NAD/NADH-Derivate
US7553615B2 (en) 2005-07-28 2009-06-30 Roche Diagnostics Operations, Inc. Compounds, methods, complexes, apparatuses and uses relating to stabile forms of NAD/NADH
EP2093284A1 (de) * 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
US20110313009A1 (en) 2008-07-17 2011-12-22 Horizon Pharma Usa, Inc. Nsaid dose unit formulations with h2-receptor antagonists and methods of use
EP2398909B1 (de) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten

Also Published As

Publication number Publication date
RU2010138610A (ru) 2012-03-27
TW200951222A (en) 2009-12-16
AU2009216908A1 (en) 2009-08-27
EP2245152B1 (de) 2019-05-01
AU2009216908B9 (en) 2012-07-05
HK1208703A1 (en) 2016-03-11
KR20130081300A (ko) 2013-07-16
US9896666B2 (en) 2018-02-20
CA2721718C (en) 2017-11-28
KR20120134142A (ko) 2012-12-11
MX2010008920A (es) 2010-09-07
US20180265849A1 (en) 2018-09-20
JP2012517816A (ja) 2012-08-09
CN101945999A (zh) 2011-01-12
US20110143416A1 (en) 2011-06-16
JP6113405B2 (ja) 2017-04-12
EP2245152A1 (de) 2010-11-03
JP5758128B2 (ja) 2015-08-05
WO2009103540A1 (de) 2009-08-27
KR101381004B1 (ko) 2014-04-08
EP2093284A1 (de) 2009-08-26
AU2009216908B2 (en) 2012-04-19
JP2011514153A (ja) 2011-05-06
US11220674B2 (en) 2022-01-11
RU2499834C2 (ru) 2013-11-27
TWI482860B (zh) 2015-05-01
AR070436A1 (es) 2010-04-07
CN101945999B (zh) 2016-06-08
KR20100118995A (ko) 2010-11-08
CA2721718A1 (en) 2009-08-27
BRPI0907839A2 (pt) 2015-08-11
CN104450659A (zh) 2015-03-25
CN102325896A (zh) 2012-01-18

Similar Documents

Publication Publication Date Title
ZA201006638B (en) Stabilization of dehydrogenases using stable coezymes
IL210462A0 (en) Pyrimidine compounds, compositions and methods of use
EP2370956A4 (en) DYNAMIC DETAIL LEVEL
ZA201103926B (en) Stable sulfoximine-insecticide compositions
GB0813384D0 (en) Stabilisation
EP2327459A4 (en) SKATEBOARD HINGE STRUCTURE
GB0807612D0 (en) New use of surfactant
GB2459968B (en) Efficiency-based determination of operational characteristics
IL213547A0 (en) Compounds and methods of use
EP2340244A4 (en) COMPOUNDS AND APPLICATION PROCEDURES
IL215823A0 (en) Stabilization of radiopharmaceuticalcompositions using ascorbic acid
IL212119A0 (en) Alkylcyclohexylethers of dihydrotetraazabenzoazulenes
PL2133419T3 (pl) Zastosowania beta-dipeptydów cyjanoficyny
IL199941A0 (en) Compositions of stable tiacumicins
EP2337520A4 (en) DEVICE FOR STABILIZING AMPUTATIONS
GB0800762D0 (en) Novel pyridazine derivatives
IL213711A0 (en) Pharmaceuticl formulation of nanonised fenofibrate
PL2145547T3 (pl) Zwiększona trwałość związków smakowo-zapachowych
TWM344943U (en) Retention structure of socket
IL189405A0 (en) Stabilization of siva2
GB201107973D0 (en) Correction of aberrations
EP2331490A4 (en) PREPARATION OF DIBUTOXYMETHANE
ZA201100141B (en) Stable crystal modifications of dopc
GB0822020D0 (en) Uses of clenbuterol
GB0821710D0 (en) Derivatives of dihydroindolone