JP2011504732A5 - - Google Patents

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Publication number
JP2011504732A5
JP2011504732A5 JP2010535283A JP2010535283A JP2011504732A5 JP 2011504732 A5 JP2011504732 A5 JP 2011504732A5 JP 2010535283 A JP2010535283 A JP 2010535283A JP 2010535283 A JP2010535283 A JP 2010535283A JP 2011504732 A5 JP2011504732 A5 JP 2011504732A5
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JP
Japan
Prior art keywords
dna polymerase
primer
group
nucleotide
polymerase
Prior art date
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Application number
JP2010535283A
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English (en)
Japanese (ja)
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JP5596554B2 (ja
JP2011504732A (ja
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Publication date
Application filed filed Critical
Priority claimed from PCT/EP2008/010036 external-priority patent/WO2009068268A1/en
Publication of JP2011504732A publication Critical patent/JP2011504732A/ja
Publication of JP2011504732A5 publication Critical patent/JP2011504732A5/ja
Application granted granted Critical
Publication of JP5596554B2 publication Critical patent/JP5596554B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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JP2010535283A 2007-11-28 2008-11-26 向上したピロリン酸分解活性化重合（ｐａｐ）能力を有する変異ｄｎａポリメラーゼ Expired - Fee Related JP5596554B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US99084707P 2007-11-28 2007-11-28
US60/990,847 2007-11-28
PCT/EP2008/010036 WO2009068268A1 (en) 2007-11-28 2008-11-26 Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability

Publications (3)

Publication Number Publication Date
JP2011504732A JP2011504732A (ja) 2011-02-17
JP2011504732A5 true JP2011504732A5 (enExample) 2011-08-18
JP5596554B2 JP5596554B2 (ja) 2014-09-24

Family

ID=40340463

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010535283A Expired - Fee Related JP5596554B2 (ja) 2007-11-28 2008-11-26 向上したピロリン酸分解活性化重合（ｐａｐ）能力を有する変異ｄｎａポリメラーゼ

Country Status (6)

Country Link
US (1) US8071536B2 (enExample)
EP (1) EP2215222B1 (enExample)
JP (1) JP5596554B2 (enExample)
CA (1) CA2706999C (enExample)
ES (1) ES2553893T3 (enExample)
WO (1) WO2009068268A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103025870B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
WO2012082753A2 (en) 2010-12-13 2012-06-21 Life Technologies Corporation Polymerization of nucleic acids using activation by polyphosphorolysis (app) reactions
CA2907423A1 (en) 2013-05-24 2014-11-27 Illumina Cambridge Limited Pyrophosphorolytic sequencing
US11921049B2 (en) * 2018-11-09 2024-03-05 Shaofeng Ding Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US6329178B1 (en) * 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
DE60139762D1 (de) * 2000-02-23 2009-10-15 Hope City Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
ATE552349T1 (de) * 2002-05-10 2012-04-15 Hope City Pyrophosphorolyse-aktivierte polymerisation (pap)
SE0202831D0 (sv) * 2002-09-25 2002-09-25 Siemens Elema Ab Apparat för bestämning av rekryterbar volym i en lunga
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8026091B2 (en) 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods

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