ES2553893T3 - ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP) - Google Patents

ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP) Download PDF

Info

Publication number
ES2553893T3
ES2553893T3 ES08855304.5T ES08855304T ES2553893T3 ES 2553893 T3 ES2553893 T3 ES 2553893T3 ES 08855304 T ES08855304 T ES 08855304T ES 2553893 T3 ES2553893 T3 ES 2553893T3
Authority
ES
Spain
Prior art keywords
nucleic acid
dna
polymerase
primer
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08855304.5T
Other languages
English (en)
Spanish (es)
Inventor
Keith A. Bauer
David Harrow Gelfand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2553893T3 publication Critical patent/ES2553893T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES08855304.5T 2007-11-28 2008-11-26 ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP) Active ES2553893T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US99084707P 2007-11-28 2007-11-28
US990847P 2007-11-28
PCT/EP2008/010036 WO2009068268A1 (en) 2007-11-28 2008-11-26 Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability

Publications (1)

Publication Number Publication Date
ES2553893T3 true ES2553893T3 (es) 2015-12-14

Family

ID=40340463

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08855304.5T Active ES2553893T3 (es) 2007-11-28 2008-11-26 ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP)

Country Status (6)

Country Link
US (1) US8071536B2 (enExample)
EP (1) EP2215222B1 (enExample)
JP (1) JP5596554B2 (enExample)
CA (1) CA2706999C (enExample)
ES (1) ES2553893T3 (enExample)
WO (1) WO2009068268A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103025870B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
WO2012082753A2 (en) 2010-12-13 2012-06-21 Life Technologies Corporation Polymerization of nucleic acids using activation by polyphosphorolysis (app) reactions
CA2907423A1 (en) 2013-05-24 2014-11-27 Illumina Cambridge Limited Pyrophosphorolytic sequencing
US11921049B2 (en) * 2018-11-09 2024-03-05 Shaofeng Ding Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US6329178B1 (en) * 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
DE60139762D1 (de) * 2000-02-23 2009-10-15 Hope City Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
ATE552349T1 (de) * 2002-05-10 2012-04-15 Hope City Pyrophosphorolyse-aktivierte polymerisation (pap)
SE0202831D0 (sv) * 2002-09-25 2002-09-25 Siemens Elema Ab Apparat för bestämning av rekryterbar volym i en lunga
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8026091B2 (en) 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods

Also Published As

Publication number Publication date
JP5596554B2 (ja) 2014-09-24
EP2215222B1 (en) 2015-09-16
US20090155802A1 (en) 2009-06-18
CA2706999C (en) 2013-01-22
CA2706999A1 (en) 2009-06-04
JP2011504732A (ja) 2011-02-17
US8071536B2 (en) 2011-12-06
WO2009068268A1 (en) 2009-06-04
EP2215222A1 (en) 2010-08-11

Similar Documents

Publication Publication Date Title
Nossal DNA synthesis on a double-stranded DNA template by the T4 bacteriophage DNA polymerase and the T4 gene 32 DNA unwinding protein
CN101778952B (zh) 使用寡核苷酸和显著缺乏5’-3’核酸外切酶活性的聚合酶抑制扩增
RU2235773C2 (ru) Модифицированная термостабильная днк-полимераза, способ её получения и применение
TW513439B (en) Altered thermostable DNA polymerases for sequencing
CN1578841B (zh) 退火控制引物及该退火控制引物的使用
EP2208789B1 (en) DNA polymerases having improved labeled nucleotide incorporation properties
Ramadan et al. Human DNA polymerase λ possesses terminal deoxyribonucleotidyl transferase activity and can elongate RNA primers: implications for novel functions
CN100590198C (zh) 改良多聚核苷酸合成的方法和成分
WO2001094562A2 (en) Cold sensitive mutant dna polymerases
AU2001266726A1 (en) Cold sensitive mutant DNA polymerases
MXPA97005961A (en) Thermostable dna polymerase, modification
EP2164955B1 (en) Mutant dna polymerases and related methods
ES2553893T3 (es) ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP)
US4401759A (en) Detection and isolation of glucagon mRNA using a synthetic oligodeoxynucleotide
CN112703248B (zh) 具有改良链置换能力的突变型dna聚合酶
EP2292767B1 (en) Polypepdides having DNA polymerase activity
CN118434848A (zh) B族dna聚合酶变体以及包含其的试剂套组
EP3768832B1 (en) Dna polymerases for efficient and effective incorporation of methylated-dntps
CN113789368A (zh) 核酸检测试剂盒、反应体系及方法
Alberts Studies on the replication of DNA
CN118086243A (zh) 一种Taq DNA聚合酶突变体及其应用
Gene Recombinant DNA technology
HK1146094B (en) Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' exonuclease activity
Dasgupta DNA/RNA sequencing
Drebin et al. Recombinant DNA Technology