CN100590198C - 改良多聚核苷酸合成的方法和成分 - Google Patents
改良多聚核苷酸合成的方法和成分 Download PDFInfo
- Publication number
- CN100590198C CN100590198C CN00815973A CN00815973A CN100590198C CN 100590198 C CN100590198 C CN 100590198C CN 00815973 A CN00815973 A CN 00815973A CN 00815973 A CN00815973 A CN 00815973A CN 100590198 C CN100590198 C CN 100590198C
- Authority
- CN
- China
- Prior art keywords
- polynucleotide
- primer
- phosphoric acid
- glu
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (14)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/404,258 | 1999-09-22 | ||
US09/404,258 US6274353B1 (en) | 1999-09-22 | 1999-09-22 | Method and compositions for improved polynucleotide synthesis |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2009101511681A Division CN101649307B (zh) | 1999-09-22 | 2000-09-22 | 改良多聚核苷酸合成的成分 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1408025A CN1408025A (zh) | 2003-04-02 |
CN100590198C true CN100590198C (zh) | 2010-02-17 |
Family
ID=23598857
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN00815973A Expired - Fee Related CN100590198C (zh) | 1999-09-22 | 2000-09-22 | 改良多聚核苷酸合成的方法和成分 |
CN2009101511681A Expired - Lifetime CN101649307B (zh) | 1999-09-22 | 2000-09-22 | 改良多聚核苷酸合成的成分 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2009101511681A Expired - Lifetime CN101649307B (zh) | 1999-09-22 | 2000-09-22 | 改良多聚核苷酸合成的成分 |
Country Status (8)
Country | Link |
---|---|
US (2) | US6274353B1 (zh) |
EP (1) | EP1224295B1 (zh) |
JP (1) | JP2003510052A (zh) |
CN (2) | CN100590198C (zh) |
AT (1) | ATE357529T1 (zh) |
AU (1) | AU7710200A (zh) |
DE (1) | DE60034053D1 (zh) |
WO (1) | WO2001021813A1 (zh) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6670127B2 (en) | 1997-09-16 | 2003-12-30 | Egea Biosciences, Inc. | Method for assembly of a polynucleotide encoding a target polypeptide |
CA2438574C (en) * | 2001-02-15 | 2009-08-11 | Hiroaki Sagawa | Method of detecting nucleotide polymorphism |
EP1275735A1 (en) | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
US6667165B2 (en) * | 2001-11-13 | 2003-12-23 | Eppendorf Ag | Method and compositions for reversible inhibition of thermostable polymerases |
US6896727B2 (en) * | 2002-06-28 | 2005-05-24 | Seh America, Inc. | Method of determining nitrogen concentration within a wafer |
WO2004003228A1 (en) * | 2002-07-01 | 2004-01-08 | Unisearch Limited | Genotyping method |
US20060228786A1 (en) * | 2003-02-06 | 2006-10-12 | Salerno John C | Polymerase-based protocols for the introduction of deletions and insertions |
US20060134624A1 (en) * | 2003-02-06 | 2006-06-22 | Salerno John C | Polymerase-based protocols for the introductions of deletions and insertions |
WO2004072246A2 (en) * | 2003-02-06 | 2004-08-26 | Rensselaer Polytechnic Institute | Polymerase-based protocols for the introduction of combinatorial deletions and insertions |
US20060257876A1 (en) * | 2003-02-06 | 2006-11-16 | Rensselaer Polytechnis Institute | Polymerase-based protocols for generating chimeric and combinatorial... |
EP1615942B1 (en) | 2003-04-01 | 2016-03-16 | Luminex Corporation | Polymerase inhibitor and method of using same |
US20050244847A1 (en) * | 2003-11-26 | 2005-11-03 | Eppendorf Ag | Methods and compositions for in vitro amplification of extrachromosomal nucleic acid |
CN101426908B (zh) * | 2004-06-09 | 2014-06-11 | 纽星实验室 | 可逆性修饰的热稳定酶组合物及其制备方法和使用方法 |
DE602007013223D1 (de) * | 2006-06-01 | 2011-04-28 | Trilink Biotechnologies San Diego | Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation |
EP3150727B1 (en) * | 2008-04-30 | 2019-07-10 | Integrated DNA Technologies Inc. | Rnase-h-based assays utilizing modified rna monomers |
US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
CN102803956A (zh) * | 2009-06-12 | 2012-11-28 | 纳内克蒂斯生物技术公司 | 用于检测在aids患者的抗逆转录病毒疗法后剩余的病毒hiv dna的高灵敏方法 |
WO2011045744A2 (en) * | 2009-10-13 | 2011-04-21 | Syntezza Molecular Detection Israel Ltd. | Methods and compositions for amplifying target sequences from nucleic acid samples |
US10378051B2 (en) | 2011-09-29 | 2019-08-13 | Illumina Cambridge Limited | Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing |
CN103205425B (zh) * | 2012-01-17 | 2015-07-01 | 北京万达因生物医学技术有限责任公司 | 抑制引物非特异性扩增的反义干扰寡核苷酸 |
AU2013232131B2 (en) * | 2012-03-13 | 2018-09-20 | Integrated Dna Technologies, Inc. | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
SG11201507512QA (en) * | 2013-03-15 | 2015-10-29 | Integrated Dna Tech Inc | Rnase h-based assays utilizing modified rna monomers |
WO2021120095A1 (zh) * | 2019-12-19 | 2021-06-24 | 深圳华大生命科学研究院 | 抗聚合酶的单克隆抗体及其应用 |
US20240102067A1 (en) * | 2022-09-26 | 2024-03-28 | Illumina, Inc. | Resynthesis Kits and Methods |
CN117070514B (zh) * | 2023-10-16 | 2024-04-26 | 吉林凯莱英医药化学有限公司 | 非天然rna的制备方法及产品 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2112302T3 (es) * | 1991-10-11 | 1998-04-01 | Behringwerke Ag | Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos. |
US5516664A (en) | 1992-12-23 | 1996-05-14 | Hyman; Edward D. | Enzymatic synthesis of repeat regions of oligonucleotides |
US5939257A (en) | 1994-04-18 | 1999-08-17 | Amersham Life Science, Inc. | Thermostable alkaline phosphatases |
CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
WO1997028279A2 (en) * | 1996-02-01 | 1997-08-07 | Behringwerke Aktiengesellschaft | Positive controls for use in polynucleotide amplification |
CA2257863A1 (en) * | 1996-06-19 | 1997-12-24 | Diversa Corporation | Thermostable phosphatases |
US6482590B1 (en) * | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
US5853997A (en) * | 1997-06-11 | 1998-12-29 | Incyte Pharmaceuticals, Inc. | Human protein phosphatase |
US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
-
1999
- 1999-09-22 US US09/404,258 patent/US6274353B1/en not_active Expired - Fee Related
-
2000
- 2000-09-22 EP EP00966814A patent/EP1224295B1/en not_active Expired - Lifetime
- 2000-09-22 WO PCT/US2000/026107 patent/WO2001021813A1/en active IP Right Grant
- 2000-09-22 AU AU77102/00A patent/AU7710200A/en not_active Abandoned
- 2000-09-22 CN CN00815973A patent/CN100590198C/zh not_active Expired - Fee Related
- 2000-09-22 AT AT00966814T patent/ATE357529T1/de not_active IP Right Cessation
- 2000-09-22 JP JP2001525371A patent/JP2003510052A/ja active Pending
- 2000-09-22 DE DE60034053T patent/DE60034053D1/de not_active Expired - Lifetime
- 2000-09-22 CN CN2009101511681A patent/CN101649307B/zh not_active Expired - Lifetime
-
2001
- 2001-05-14 US US09/853,701 patent/US6699981B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CN101649307A (zh) | 2010-02-17 |
ATE357529T1 (de) | 2007-04-15 |
US20020182686A1 (en) | 2002-12-05 |
WO2001021813A1 (en) | 2001-03-29 |
US6274353B1 (en) | 2001-08-14 |
DE60034053D1 (de) | 2007-05-03 |
US6699981B2 (en) | 2004-03-02 |
CN1408025A (zh) | 2003-04-02 |
EP1224295A1 (en) | 2002-07-24 |
CN101649307B (zh) | 2011-11-30 |
JP2003510052A (ja) | 2003-03-18 |
EP1224295B1 (en) | 2007-03-21 |
AU7710200A (en) | 2001-04-24 |
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PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: GUANGZHOU FULENGEN CO., LTD. Free format text: FORMER OWNER: GENECOPOEIA INC. Effective date: 20110608 |
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C41 | Transfer of patent application or patent right or utility model | ||
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: STATE OF MARYLAND, THE USA TO: 510663 8/F, AREA D, INTERNATIONAL ENTERPRISE INCUBATOR, GUANGZHOU SCIENCE CITY, GUANGZHOU CITY, GUANGDONG PROVINCE |
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TR01 | Transfer of patent right |
Effective date of registration: 20110608 Address after: 510663, Guangdong City, Guangzhou province Guangzhou Science City International Business Incubator D District 8 floor Patentee after: Guangzhou FulenGen Co., Ltd. Address before: Ma Li Lanzhou USA Patentee before: Jinkebei Gene Technology Co. |
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100217 Termination date: 20160922 |
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CF01 | Termination of patent right due to non-payment of annual fee |