CA2706999C - Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability - Google Patents

Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability Download PDF

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Publication number
CA2706999C
CA2706999C CA2706999A CA2706999A CA2706999C CA 2706999 C CA2706999 C CA 2706999C CA 2706999 A CA2706999 A CA 2706999A CA 2706999 A CA2706999 A CA 2706999A CA 2706999 C CA2706999 C CA 2706999C
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CA
Canada
Prior art keywords
polymerase
primer
dna polymerase
seq
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2706999A
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English (en)
French (fr)
Other versions
CA2706999A1 (en
Inventor
Keith A. Bauer
David Harrow Gelfand
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication date
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Publication of CA2706999A1 publication Critical patent/CA2706999A1/en
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Publication of CA2706999C publication Critical patent/CA2706999C/en
Expired - Fee Related legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2706999A 2007-11-28 2008-11-26 Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability Expired - Fee Related CA2706999C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US99084707P 2007-11-28 2007-11-28
US60/990,847 2007-11-28
PCT/EP2008/010036 WO2009068268A1 (en) 2007-11-28 2008-11-26 Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability

Publications (2)

Publication Number Publication Date
CA2706999A1 CA2706999A1 (en) 2009-06-04
CA2706999C true CA2706999C (en) 2013-01-22

Family

ID=40340463

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2706999A Expired - Fee Related CA2706999C (en) 2007-11-28 2008-11-26 Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability

Country Status (6)

Country Link
US (1) US8071536B2 (enExample)
EP (1) EP2215222B1 (enExample)
JP (1) JP5596554B2 (enExample)
CA (1) CA2706999C (enExample)
ES (1) ES2553893T3 (enExample)
WO (1) WO2009068268A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103025870B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
WO2012082753A2 (en) 2010-12-13 2012-06-21 Life Technologies Corporation Polymerization of nucleic acids using activation by polyphosphorolysis (app) reactions
CA2907423A1 (en) 2013-05-24 2014-11-27 Illumina Cambridge Limited Pyrophosphorolytic sequencing
US11921049B2 (en) * 2018-11-09 2024-03-05 Shaofeng Ding Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
US6329178B1 (en) * 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
DE60139762D1 (de) * 2000-02-23 2009-10-15 Hope City Pyrophosphorolyse-aktivierte polymerisierung (pap): anwendung für allel-spezifische amplifizierung und sequenzbestimmung von nukleinsäuren
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
ATE552349T1 (de) * 2002-05-10 2012-04-15 Hope City Pyrophosphorolyse-aktivierte polymerisation (pap)
SE0202831D0 (sv) * 2002-09-25 2002-09-25 Siemens Elema Ab Apparat för bestämning av rekryterbar volym i en lunga
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8026091B2 (en) 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods

Also Published As

Publication number Publication date
JP5596554B2 (ja) 2014-09-24
EP2215222B1 (en) 2015-09-16
US20090155802A1 (en) 2009-06-18
ES2553893T3 (es) 2015-12-14
CA2706999A1 (en) 2009-06-04
JP2011504732A (ja) 2011-02-17
US8071536B2 (en) 2011-12-06
WO2009068268A1 (en) 2009-06-04
EP2215222A1 (en) 2010-08-11

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Effective date: 20211126