JP2010539956A5 - - Google Patents
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- Publication number
- JP2010539956A5 JP2010539956A5 JP2010527295A JP2010527295A JP2010539956A5 JP 2010539956 A5 JP2010539956 A5 JP 2010539956A5 JP 2010527295 A JP2010527295 A JP 2010527295A JP 2010527295 A JP2010527295 A JP 2010527295A JP 2010539956 A5 JP2010539956 A5 JP 2010539956A5
- Authority
- JP
- Japan
- Prior art keywords
- primer
- oligonucleotide
- facilitator
- sequence
- initiator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091034117 Oligonucleotide Proteins 0.000 claims 57
- 239000013615 primer Substances 0.000 claims 57
- 238000000034 method Methods 0.000 claims 31
- 239000003999 initiator Substances 0.000 claims 22
- 230000015572 biosynthetic process Effects 0.000 claims 20
- 238000003786 synthesis reaction Methods 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 17
- 108020004707 nucleic acids Proteins 0.000 claims 16
- 150000007523 nucleic acids Chemical class 0.000 claims 16
- 102000039446 nucleic acids Human genes 0.000 claims 16
- 230000004048 modification Effects 0.000 claims 15
- 238000012986 modification Methods 0.000 claims 15
- 239000002773 nucleotide Substances 0.000 claims 15
- 230000001419 dependent effect Effects 0.000 claims 14
- 102000005877 Peptide Initiation Factors Human genes 0.000 claims 10
- 108010044843 Peptide Initiation Factors Proteins 0.000 claims 10
- 230000000295 complement effect Effects 0.000 claims 10
- 230000003321 amplification Effects 0.000 claims 9
- 238000003199 nucleic acid amplification method Methods 0.000 claims 9
- 239000003155 DNA primer Substances 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 4
- 230000002255 enzymatic effect Effects 0.000 claims 4
- 238000005382 thermal cycling Methods 0.000 claims 4
- 208000035657 Abasia Diseases 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 238000007792 addition Methods 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 2
- 238000010348 incorporation Methods 0.000 claims 2
- 125000006850 spacer group Chemical group 0.000 claims 2
- 230000007067 DNA methylation Effects 0.000 claims 1
- 108091093037 Peptide nucleic acid Proteins 0.000 claims 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 239000012472 biological sample Substances 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 239000005546 dideoxynucleotide Substances 0.000 claims 1
- 230000008034 disappearance Effects 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- -1 nucleotide amines Chemical class 0.000 claims 1
- 230000037452 priming Effects 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2007905445A AU2007905445A0 (en) | 2007-10-04 | Nucleic acid amplification | |
| AU2007905445 | 2007-10-04 | ||
| PCT/AU2008/001475 WO2009043112A1 (en) | 2007-10-04 | 2008-10-03 | Nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010539956A JP2010539956A (ja) | 2010-12-24 |
| JP2010539956A5 true JP2010539956A5 (OSRAM) | 2011-11-17 |
| JP5535921B2 JP5535921B2 (ja) | 2014-07-02 |
Family
ID=40525777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010527295A Active JP5535921B2 (ja) | 2007-10-04 | 2008-10-03 | 核酸増幅 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8455197B2 (OSRAM) |
| EP (1) | EP2207896B1 (OSRAM) |
| JP (1) | JP5535921B2 (OSRAM) |
| CN (1) | CN101889096B (OSRAM) |
| AU (1) | AU2008307153B2 (OSRAM) |
| ES (1) | ES2460896T3 (OSRAM) |
| WO (1) | WO2009043112A1 (OSRAM) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961562B2 (en) | 2016-09-06 | 2021-03-30 | Swift Biosciences, Inc. | Normalization of NGS library concentration |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2271772B1 (en) * | 2008-03-11 | 2014-07-16 | Sequenom, Inc. | Nucleic acid-based tests for prenatal gender determination |
| US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| US8962247B2 (en) * | 2008-09-16 | 2015-02-24 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses |
| EP2454381A1 (en) * | 2009-07-16 | 2012-05-23 | Abbott Laboratories | A method for amplification of nucleic acids |
| EP3088532B1 (en) | 2009-12-22 | 2019-10-30 | Sequenom, Inc. | Processes and kits for identifying aneuploidy |
| CA2834218C (en) | 2011-04-29 | 2021-02-16 | Sequenom, Inc. | Quantification of a minority nucleic acid species using inhibitory oligonucleotides |
| US20130157885A1 (en) * | 2011-11-17 | 2013-06-20 | Rheonix, Inc. | System and methods for selective molecular analysis |
| EP3401399B1 (en) | 2012-03-02 | 2020-04-22 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| MX368034B (es) | 2012-05-11 | 2019-09-17 | Clinical Genomics Pty Ltd | Panel de marcadores genéticos para el diagnóstico de cáncer colorrectal. |
| US10504613B2 (en) | 2012-12-20 | 2019-12-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
| JP2015521862A (ja) | 2012-07-13 | 2015-08-03 | セクエノム， インコーポレイテッド | 非侵襲性の出生前診断に有用な母体サンプル由来の胎児核酸のメチル化に基づく富化のためのプロセスおよび組成物 |
| US20140308710A1 (en) * | 2012-12-13 | 2014-10-16 | Synthetic Genomics, Inc. | Peg-mediated assembly of nucleic acid molecules |
| EP3597774A1 (en) | 2013-03-13 | 2020-01-22 | Sequenom, Inc. | Primers for dna methylation analysis |
| CN113832216A (zh) * | 2013-03-15 | 2021-12-24 | 莱尔·J·阿诺德 | 使用发卡寡核苷酸扩增核酸的方法 |
| EP2971134B1 (en) * | 2013-03-15 | 2023-10-25 | Aegea Biotechnologies, Inc. | Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence |
| EP3030680B1 (en) * | 2013-08-09 | 2018-10-03 | Luminex Corporation | Melt discrimination and multiplexing in nucleic acid assays |
| WO2015138774A1 (en) | 2014-03-13 | 2015-09-17 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| RU2708992C2 (ru) * | 2014-05-19 | 2019-12-12 | Уильям Марш Райс Юниверсити | Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора |
| CN104131070A (zh) * | 2014-05-22 | 2014-11-05 | 苏州工业园区为真生物医药科技有限公司 | 一种对基因甲基化检测的方法 |
| WO2016061624A1 (en) | 2014-10-20 | 2016-04-28 | Commonwealth Scientific And Industrial Research Organisation | Genome methylation analysis |
| CN105331727B (zh) * | 2015-12-01 | 2019-09-03 | 湖南宏雅基因技术有限公司 | 一种人外周血循环肿瘤DNA中septin 9基因甲基化的检测试剂盒 |
| GB201612011D0 (en) | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
| JP6999645B2 (ja) * | 2016-08-02 | 2022-02-04 | エフ．ホフマン－ラ ロシュ アーゲー | 核酸の増幅及び検出／定量の効率を改良するためのヘルパーオリゴヌクレオチド |
| DE102016120124B8 (de) * | 2016-10-21 | 2018-08-23 | Gna Biosolutions Gmbh | Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens |
| US10787699B2 (en) * | 2017-02-08 | 2020-09-29 | Microsoft Technology Licensing, Llc | Generating pluralities of primer and payload designs for retrieval of stored nucleotides |
| EP3530754A1 (de) * | 2018-02-26 | 2019-08-28 | AGCT GmbH | Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität |
| CN112074612A (zh) * | 2018-02-26 | 2020-12-11 | Agct有限公司 | 一种具有更高特异性的核酸扩增方法 |
| EP3530753A1 (de) * | 2018-02-26 | 2019-08-28 | AGCT GmbH | Verfahren zur primer-verlängerungsreaktion mit verbesserter spezifität |
| US20210071243A1 (en) * | 2018-02-26 | 2021-03-11 | Agct Gmbh | Method for primer extension reaction with improved specificity |
| EP3759246A1 (de) * | 2018-02-28 | 2021-01-06 | AGCT GmbH | Verfahren zur amplifikation einer nukleinsäure mit verbesserter spezifität |
| CN114075595B (zh) * | 2021-11-22 | 2023-11-17 | 上海交通大学 | 甲基化检测组合物、试剂盒及方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6335184B1 (en) * | 1993-07-23 | 2002-01-01 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
| US6057134A (en) * | 1996-10-07 | 2000-05-02 | Ambion, Inc. | Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides |
| EP0866071B1 (en) * | 1997-03-20 | 2004-10-20 | F. Hoffmann-La Roche Ag | Modified primers |
| US7205129B1 (en) * | 2000-02-28 | 2007-04-17 | Qiagen Gmbh | Method for reducing artifacts in nucleic acid amplification |
| JPWO2003016569A1 (ja) * | 2001-08-20 | 2004-12-02 | タカラバイオ株式会社 | 核酸の増幅方法 |
| US7629152B2 (en) * | 2002-03-01 | 2009-12-08 | Integrated Dna Technologies, Inc. | Methods for amplifying polymeric nucleic acids |
| AU2003213652A1 (en) * | 2002-03-01 | 2003-09-16 | Integrated Dna Technologies, Inc. | Polynomial amplification of nucleic acids |
| US20050153333A1 (en) * | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| US20060014183A1 (en) * | 2004-06-10 | 2006-01-19 | Pfundheller Henrik M | Extendable probes |
| CN101443458B (zh) | 2006-03-28 | 2014-04-02 | 联邦科学及工业研究组织 | 扩增dna片段 |
| WO2008064687A1 (en) * | 2006-11-27 | 2008-06-05 | Fluimedix | Fidelity enhanced allele specific amplification method |
-
2008
- 2008-10-03 EP EP08800110.2A patent/EP2207896B1/en active Active
- 2008-10-03 WO PCT/AU2008/001475 patent/WO2009043112A1/en not_active Ceased
- 2008-10-03 US US12/681,603 patent/US8455197B2/en not_active Expired - Fee Related
- 2008-10-03 AU AU2008307153A patent/AU2008307153B2/en not_active Ceased
- 2008-10-03 JP JP2010527295A patent/JP5535921B2/ja active Active
- 2008-10-03 CN CN200880119335.8A patent/CN101889096B/zh not_active Expired - Fee Related
- 2008-10-03 ES ES08800110.2T patent/ES2460896T3/es active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961562B2 (en) | 2016-09-06 | 2021-03-30 | Swift Biosciences, Inc. | Normalization of NGS library concentration |
| US12371731B2 (en) | 2016-09-06 | 2025-07-29 | Integrated Dna Technologies, Inc. | Normalization of NGS library concentration |
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