JP2010518879A - 細胞培養において気泡の形成を減少させるための装置および方法 - Google Patents
細胞培養において気泡の形成を減少させるための装置および方法 Download PDFInfo
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- JP2010518879A JP2010518879A JP2009551990A JP2009551990A JP2010518879A JP 2010518879 A JP2010518879 A JP 2010518879A JP 2009551990 A JP2009551990 A JP 2009551990A JP 2009551990 A JP2009551990 A JP 2009551990A JP 2010518879 A JP2010518879 A JP 2010518879A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/08—Flasks
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
Description
106,306,506 バリヤプレート
109,309,409 開口
110 上板
111 細胞増殖チャンバ
112 側壁
113 気体透過性膜または底面
114 端壁
117 細胞
118 気管空間
120 底部トレイ
121,521,621 首付き開口部
122,423,523 隔壁
127 細胞培地
304,504,604 マニホールド
Claims (5)
- 細胞培養容器を通して液体の流入および気体の流出を方向付けるためのマニホールドであって、
開口部および内面を有する首付き開口部、
流入肩部と流出肩部、および
細胞培養容器に取り付けられるように構築され配置されたバリヤプレート、
を備え、
前記流入肩部と流出肩部が、前記バリヤプレートおよび前記首に取り付けられ、
前記流入肩部と流出肩部の各々が内面を有し、
前記首の内面が、前記流入肩部および前記流出肩部の内面に隣接し、かつ連続しており、
前記流入肩部および前記流出肩部が、前記バリやプレートを通る少なくとも1つの開口を有し、
それによって、前記首付き開口部に導入される液体が、前記首付き開口部を通って前記流入肩部に入り、流入開口を通って前記細胞培養容器に流入し、かつ該細胞培養容器に進入する前記液体により置き換えられる気体が、該細胞培養容器から、前記流出肩部の開口を通り、該流出肩部を通り、前記首付き開口部に導入を通って流出することを特徴とするマニホールド。 - 前記細胞培養容器と一体になっていることを特徴とする請求項1記載のマニホールド。
- 前記細胞培養容器に取外し可能に取り付けられることを特徴とする請求項1記載のマニホールド。
- 前記培養容器が多層フラスコであることを特徴とする請求項1から3いずれか1項記載の培養容器。
- 前記首付き開口部がさらに隔壁を備えることを特徴とする請求項1から4いずれか1項記載の培養容器。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/710,815 US7897379B2 (en) | 2007-02-26 | 2007-02-26 | Device and method for reducing bubble formation in cell culture |
US11/710,815 | 2007-02-26 | ||
PCT/US2008/001885 WO2008106012A1 (en) | 2007-02-26 | 2008-02-13 | Device and method for reducing bubble formation in cell culture |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2010518879A true JP2010518879A (ja) | 2010-06-03 |
JP5088756B2 JP5088756B2 (ja) | 2012-12-05 |
Family
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Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009551990A Active JP5088756B2 (ja) | 2007-02-26 | 2008-02-13 | 細胞培養において気泡の形成を減少させるための装置および方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US7897379B2 (ja) |
EP (1) | EP2118259B1 (ja) |
JP (1) | JP5088756B2 (ja) |
AT (1) | ATE506430T1 (ja) |
DE (1) | DE602008006361D1 (ja) |
WO (1) | WO2008106012A1 (ja) |
Cited By (17)
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JP2013198438A (ja) * | 2012-03-26 | 2013-10-03 | Terumo Corp | 細胞培養装置 |
JP2014500709A (ja) * | 2010-10-12 | 2014-01-16 | ナルジェ・エヌユーエヌシー・インターナショナル・コーポレーション | ポートを備えたベント可能な閉塞部材 |
JP2014143998A (ja) * | 2013-01-18 | 2014-08-14 | Eppendorf Ag | 培養瓶 |
WO2014148507A1 (ja) * | 2013-03-22 | 2014-09-25 | 株式会社ジェイ・エム・エス | 細胞培養容器 |
WO2014148508A1 (ja) * | 2013-03-22 | 2014-09-25 | 株式会社ジェイ・エム・エス | 細胞培養容器 |
WO2015033507A1 (ja) * | 2013-09-06 | 2015-03-12 | 住友ベークライト株式会社 | 細胞培養フラスコおよび細胞培養フラスコの製造方法 |
KR101532282B1 (ko) * | 2013-12-13 | 2015-06-30 | 한국원자력연구원 | 입자빔을 이용한 동물 세포 사멸 실험용 용기 |
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US11345880B2 (en) | 2017-07-14 | 2022-05-31 | Corning Incorporated | 3D cell culture vessels for manual or automatic media exchange |
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JP5088756B2 (ja) | 2012-12-05 |
DE602008006361D1 (de) | 2011-06-01 |
ATE506430T1 (de) | 2011-05-15 |
WO2008106012A1 (en) | 2008-09-04 |
EP2118259B1 (en) | 2011-04-20 |
US7897379B2 (en) | 2011-03-01 |
EP2118259A1 (en) | 2009-11-18 |
US20080206857A1 (en) | 2008-08-28 |
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