JP2010506573A5 - - Google Patents
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- Publication number
- JP2010506573A5 JP2010506573A5 JP2009532720A JP2009532720A JP2010506573A5 JP 2010506573 A5 JP2010506573 A5 JP 2010506573A5 JP 2009532720 A JP2009532720 A JP 2009532720A JP 2009532720 A JP2009532720 A JP 2009532720A JP 2010506573 A5 JP2010506573 A5 JP 2010506573A5
- Authority
- JP
- Japan
- Prior art keywords
- polymerase
- nucleotide
- oligonucleotide
- biocatalyst
- dna polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002773 nucleotide Substances 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 20
- 108091034117 Oligonucleotide Proteins 0.000 claims 14
- 102000004190 Enzymes Human genes 0.000 claims 11
- 108090000790 Enzymes Proteins 0.000 claims 11
- 239000011942 biocatalyst Substances 0.000 claims 11
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 10
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 10
- 238000000034 method Methods 0.000 claims 9
- 229910052760 oxygen Inorganic materials 0.000 claims 8
- 229910052717 sulfur Inorganic materials 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 7
- 102000039446 nucleic acids Human genes 0.000 claims 7
- 150000007523 nucleic acids Chemical class 0.000 claims 7
- 102100034343 Integrase Human genes 0.000 claims 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 4
- 102200120949 rs199517715 Human genes 0.000 claims 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- 229910052711 selenium Inorganic materials 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 108010006785 Taq Polymerase Proteins 0.000 claims 2
- 239000007853 buffer solution Substances 0.000 claims 2
- -1 clinical specimen Substances 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 239000011541 reaction mixture Substances 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 108091093088 Amplicon Proteins 0.000 claims 1
- 108010017826 DNA Polymerase I Proteins 0.000 claims 1
- 102000004594 DNA Polymerase I Human genes 0.000 claims 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- 101001066878 Homo sapiens Polyribonucleotide nucleotidyltransferase 1, mitochondrial Proteins 0.000 claims 1
- 241001655327 Micrococcales Species 0.000 claims 1
- 102000002681 Polyribonucleotide nucleotidyltransferase Human genes 0.000 claims 1
- 102000009572 RNA Polymerase II Human genes 0.000 claims 1
- 108010009460 RNA Polymerase II Proteins 0.000 claims 1
- 108010065868 RNA polymerase SP6 Proteins 0.000 claims 1
- 108091028664 Ribonucleotide Proteins 0.000 claims 1
- 241000555745 Sciuridae Species 0.000 claims 1
- 101710137500 T7 RNA polymerase Proteins 0.000 claims 1
- 241000589596 Thermus Species 0.000 claims 1
- 241000589497 Thermus sp. Species 0.000 claims 1
- 241000589499 Thermus thermophilus Species 0.000 claims 1
- 125000003172 aldehyde group Chemical group 0.000 claims 1
- 125000003342 alkenyl group Chemical group 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 125000000304 alkynyl group Chemical group 0.000 claims 1
- 125000003118 aryl group Chemical group 0.000 claims 1
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000006184 cosolvent Substances 0.000 claims 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims 1
- 235000011180 diphosphates Nutrition 0.000 claims 1
- 125000000623 heterocyclic group Chemical group 0.000 claims 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 claims 1
- 239000000138 intercalating agent Substances 0.000 claims 1
- 238000004020 luminiscence type Methods 0.000 claims 1
- 229910021645 metal ion Inorganic materials 0.000 claims 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 239000003607 modifier Substances 0.000 claims 1
- 125000000896 monocarboxylic acid group Chemical group 0.000 claims 1
- 125000002950 monocyclic group Chemical group 0.000 claims 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 239000002336 ribonucleotide Substances 0.000 claims 1
- 125000002652 ribonucleotide group Chemical group 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 230000002000 scavenging effect Effects 0.000 claims 1
- 230000026683 transduction Effects 0.000 claims 1
- 238000010361 transduction Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/583,606 | 2006-10-18 | ||
| US11/583,606 US7745125B2 (en) | 2004-06-28 | 2006-10-18 | 2′-terminator related pyrophosphorolysis activated polymerization |
| PCT/EP2007/008997 WO2008046602A2 (en) | 2006-10-18 | 2007-10-17 | 2'-terminator related pyrophosphorolysis activated polymerization |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010506573A JP2010506573A (ja) | 2010-03-04 |
| JP2010506573A5 true JP2010506573A5 (https=) | 2010-09-16 |
| JP5421112B2 JP5421112B2 (ja) | 2014-02-19 |
Family
ID=39283915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009532720A Expired - Fee Related JP5421112B2 (ja) | 2006-10-18 | 2007-10-17 | ２’−終止関連の加ピロリン酸分解活性化された重合 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7745125B2 (https=) |
| EP (1) | EP2076606B1 (https=) |
| JP (1) | JP5421112B2 (https=) |
| CN (2) | CN105274169A (https=) |
| AT (1) | ATE542922T1 (https=) |
| CA (1) | CA2664229C (https=) |
| ES (1) | ES2381505T3 (https=) |
| HK (1) | HK1220733A1 (https=) |
| WO (1) | WO2008046602A2 (https=) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60322044D1 (de) | 2002-02-21 | 2008-08-21 | Asm Scient Inc | Rekombinase-polymerase-amplifikation |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US7947817B2 (en) * | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US7572581B2 (en) * | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| US7928207B2 (en) * | 2004-06-28 | 2011-04-19 | Roche Molecular Systems, Inc | Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides |
| JP5027126B2 (ja) | 2005-07-25 | 2012-09-19 | アリーア サン ディエゴ， インコーポレイテッド | リコンビナーゼポリメラーゼ増幅を多重化するための方法 |
| CN101346472B (zh) * | 2005-12-21 | 2013-04-24 | 霍夫曼—拉罗奇有限公司 | 用可逆2’-修饰的核苷酸进行的序列测定和基因分型 |
| US8071308B2 (en) | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| ES2553893T3 (es) * | 2007-11-28 | 2015-12-14 | F. Hoffmann-La Roche Ag | ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP) |
| US10150990B2 (en) | 2008-04-21 | 2018-12-11 | Roche Molecular Systems, Inc. | Ribonucleotide tag nucleic acid detection |
| JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質 |
| ES3022638T3 (en) | 2009-06-05 | 2025-05-28 | Abbott Diagnostics Scarborough Inc | Recombinase polymerase amplification reagents |
| JP2013501523A (ja) * | 2009-08-11 | 2013-01-17 | レスポンス ジェネティクス，インコーポレイティド | Ｂｒａｆ変異の検出に有用な方法、プライマー、プローブ、及びキット |
| US8409802B2 (en) * | 2009-08-14 | 2013-04-02 | Roche Molecular Systems, Inc. | Format of probes to detect nucleic acid differences |
| US8932813B2 (en) | 2010-12-13 | 2015-01-13 | Life Technologies Corporation | Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions |
| BR112013025758B1 (pt) | 2011-04-07 | 2021-04-06 | Abbott Diagnostics Scarborough, Inc. | Monitoramento de misturas de amplificação de recombinase polimerase |
| ES2625288T3 (es) | 2011-04-15 | 2017-07-19 | The Johns Hopkins University | Sistema de secuenciación segura |
| MD20140044A2 (ro) | 2011-11-11 | 2014-08-31 | Pfizer Inc. | 2-Tiopirimidinone şi utilizarea lor pentru tratamentul afecţiunilor cardiovasculare |
| KR101545848B1 (ko) * | 2012-04-09 | 2015-08-21 | (주)바이오니아 | 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법 |
| AU2013338393C1 (en) | 2012-10-29 | 2024-07-25 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| US11384377B2 (en) | 2013-04-02 | 2022-07-12 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| US8808989B1 (en) * | 2013-04-02 | 2014-08-19 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| US9771613B2 (en) | 2013-04-02 | 2017-09-26 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acid |
| US10683536B2 (en) | 2013-04-02 | 2020-06-16 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| US11331643B2 (en) | 2013-04-02 | 2022-05-17 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| US9279149B2 (en) | 2013-04-02 | 2016-03-08 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| AU2016257179A1 (en) | 2015-05-05 | 2017-11-02 | Pfizer Inc. | 2-thiopyrimidinones |
| CN104862401B (zh) * | 2015-05-29 | 2017-10-31 | 沈阳优吉诺生物科技有限公司 | 检测kras基因热点突变位点的引物、试剂盒及其pcr方法 |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| IL319255A (en) | 2017-08-07 | 2025-04-01 | Univ Johns Hopkins | Methods and materials for cancer assessment and treatment |
| EA202091673A1 (ru) | 2018-01-29 | 2021-02-03 | Ст. Джуд Чилдрен'С Рисерч Хоспитал, Инк. | Способ амплификации нуклеиновых кислот |
| ES2874753T3 (es) * | 2018-07-19 | 2021-11-05 | Biofidelity Ltd | Método mejorado de detección de secuencia de polinucleótidos |
| EP4004230A4 (en) * | 2019-07-31 | 2023-08-09 | Bioskryb Genomics, Inc. | GENETIC MUTATION ANALYSIS |
| GB201919186D0 (en) * | 2019-12-23 | 2020-02-05 | Biofidelity Ltd | Simplified polynucleotide sequence detection method |
| JP2023513606A (ja) | 2020-02-14 | 2023-03-31 | ザ・ジョンズ・ホプキンス・ユニバーシティー | 核酸を評価するための方法および材料 |
| US11634754B2 (en) | 2021-04-15 | 2023-04-25 | Biofidelity Ltd. | Nucleic acid enrichment and detection |
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| NL41678C (https=) | 1931-07-27 | |||
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| US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
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| CN101346472B (zh) | 2005-12-21 | 2013-04-24 | 霍夫曼—拉罗奇有限公司 | 用可逆2’-修饰的核苷酸进行的序列测定和基因分型 |
-
2006
- 2006-10-18 US US11/583,606 patent/US7745125B2/en not_active Expired - Fee Related
-
2007
- 2007-10-17 AT AT07819065T patent/ATE542922T1/de active
- 2007-10-17 ES ES07819065T patent/ES2381505T3/es active Active
- 2007-10-17 EP EP20070819065 patent/EP2076606B1/en not_active Not-in-force
- 2007-10-17 CN CN201510684383.3A patent/CN105274169A/zh active Pending
- 2007-10-17 JP JP2009532720A patent/JP5421112B2/ja not_active Expired - Fee Related
- 2007-10-17 CN CNA2007800386518A patent/CN101528945A/zh active Pending
- 2007-10-17 CA CA 2664229 patent/CA2664229C/en not_active Expired - Fee Related
- 2007-10-17 WO PCT/EP2007/008997 patent/WO2008046602A2/en not_active Ceased
-
2016
- 2016-07-22 HK HK16108797.0A patent/HK1220733A1/zh unknown
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