HK1220733A1 - 2’-终止子有关的焦磷酸解作用激活的聚合 - Google Patents

2’-终止子有关的焦磷酸解作用激活的聚合 Download PDF

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Publication number
HK1220733A1
HK1220733A1 HK16108797.0A HK16108797A HK1220733A1 HK 1220733 A1 HK1220733 A1 HK 1220733A1 HK 16108797 A HK16108797 A HK 16108797A HK 1220733 A1 HK1220733 A1 HK 1220733A1
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Hong Kong
Prior art keywords
polymerase
nucleic acid
nucleotide
oligonucleotide
primer
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HK16108797.0A
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English (en)
Chinese (zh)
Inventor
Harrow Gelfand David
A. Bauer Keith
P. Gupta Amar
Bodepudi Veeraiah
Niemiec John
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F. Hoffmann-La Roche Ag
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Publication of HK1220733A1 publication Critical patent/HK1220733A1/zh

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
HK16108797.0A 2006-10-18 2016-07-22 2’-终止子有关的焦磷酸解作用激活的聚合 HK1220733A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/583,606 US7745125B2 (en) 2004-06-28 2006-10-18 2′-terminator related pyrophosphorolysis activated polymerization
US11/583606 2006-10-18

Publications (1)

Publication Number Publication Date
HK1220733A1 true HK1220733A1 (zh) 2017-05-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
HK16108797.0A HK1220733A1 (zh) 2006-10-18 2016-07-22 2’-终止子有关的焦磷酸解作用激活的聚合

Country Status (9)

Country Link
US (1) US7745125B2 (https=)
EP (1) EP2076606B1 (https=)
JP (1) JP5421112B2 (https=)
CN (2) CN105274169A (https=)
AT (1) ATE542922T1 (https=)
CA (1) CA2664229C (https=)
ES (1) ES2381505T3 (https=)
HK (1) HK1220733A1 (https=)
WO (1) WO2008046602A2 (https=)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7928207B2 (en) * 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
CN101346472B (zh) * 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
ES2553893T3 (es) * 2007-11-28 2015-12-14 F. Hoffmann-La Roche Ag ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP)
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
JP5847076B2 (ja) 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Dnaグリコシラーゼ/リアーゼおよびapエンドヌクレアーゼ基質
ES3022638T3 (en) 2009-06-05 2025-05-28 Abbott Diagnostics Scarborough Inc Recombinase polymerase amplification reagents
JP2013501523A (ja) * 2009-08-11 2013-01-17 レスポンス ジェネティクス,インコーポレイティド Braf変異の検出に有用な方法、プライマー、プローブ、及びキット
US8409802B2 (en) * 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
US8932813B2 (en) 2010-12-13 2015-01-13 Life Technologies Corporation Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions
BR112013025758B1 (pt) 2011-04-07 2021-04-06 Abbott Diagnostics Scarborough, Inc. Monitoramento de misturas de amplificação de recombinase polimerase
ES2625288T3 (es) 2011-04-15 2017-07-19 The Johns Hopkins University Sistema de secuenciación segura
MD20140044A2 (ro) 2011-11-11 2014-08-31 Pfizer Inc. 2-Tiopirimidinone şi utilizarea lor pentru tratamentul afecţiunilor cardiovasculare
KR101545848B1 (ko) * 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
AU2013338393C1 (en) 2012-10-29 2024-07-25 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US8808989B1 (en) * 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
AU2016257179A1 (en) 2015-05-05 2017-11-02 Pfizer Inc. 2-thiopyrimidinones
CN104862401B (zh) * 2015-05-29 2017-10-31 沈阳优吉诺生物科技有限公司 检测kras基因热点突变位点的引物、试剂盒及其pcr方法
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
IL319255A (en) 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
EA202091673A1 (ru) 2018-01-29 2021-02-03 Ст. Джуд Чилдрен'С Рисерч Хоспитал, Инк. Способ амплификации нуклеиновых кислот
ES2874753T3 (es) * 2018-07-19 2021-11-05 Biofidelity Ltd Método mejorado de detección de secuencia de polinucleótidos
EP4004230A4 (en) * 2019-07-31 2023-08-09 Bioskryb Genomics, Inc. GENETIC MUTATION ANALYSIS
GB201919186D0 (en) * 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
JP2023513606A (ja) 2020-02-14 2023-03-31 ザ・ジョンズ・ホプキンス・ユニバーシティー 核酸を評価するための方法および材料
US11634754B2 (en) 2021-04-15 2023-04-25 Biofidelity Ltd. Nucleic acid enrichment and detection

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL41678C (https=) 1931-07-27
US3338882A (en) 1965-06-28 1967-08-29 Upjohn Co 2'-and 3'-phosphates of 1-beta-d-arabinofuranosylcytosine
JPS5290693A (en) 1977-02-21 1977-07-30 Kyowa Hakko Kogyo Co Ltd Preparation of guanine nucleotide by fermentation
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
CA1219824A (en) 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
JPS5985298A (ja) 1982-11-04 1984-05-17 Yamasa Shoyu Co Ltd 3′−デオキシグアノシンの製造法
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
DE3803021A1 (de) 1988-02-02 1989-08-10 Geiger Reinhard Atp-derivate
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JPH0778074B2 (ja) 1989-09-29 1995-08-23 堺化学工業株式会社 ヌクレオシド―2’,5’‐及び3’,5’‐二リン酸の製造方法
ATE269870T1 (de) 1989-10-24 2004-07-15 Isis Pharmaceuticals Inc 2'-modifizierte oligonukleotide
US5914396A (en) 1990-01-11 1999-06-22 Isis Pharmaceuticals, Inc. 2'-O-modified nucleosides and phosphoramidites
US6005087A (en) 1995-06-06 1999-12-21 Isis Pharmaceuticals, Inc. 2'-modified oligonucleotides
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
AU4848793A (en) 1992-09-11 1994-04-12 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
CA2153387A1 (en) 1993-01-07 1994-07-21 Hubert Koester Dna sequencing by mass spectrometry
US5532130A (en) 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
US5798340A (en) 1993-09-17 1998-08-25 Gilead Sciences, Inc. Nucleotide analogs
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
ES2203635T3 (es) 1994-04-27 2004-04-16 Novartis Ag Nucleosidos y oligonucleotidos con grupos 2'-eter.
JPH09316093A (ja) 1996-03-28 1997-12-09 Sankyo Co Ltd ポリリン酸誘導体
CZ293215B6 (cs) 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
US6699668B1 (en) 1997-01-15 2004-03-02 Xzillion Gmbh & Co. Mass label linked hybridisation probes
GB9718921D0 (en) 1997-09-05 1997-11-12 Brax Genomics Ltd Catalytically generated mass labels
US20030083273A1 (en) 1999-04-20 2003-05-01 Tod M. Woolf Antisense oligomers
US6147200A (en) 1999-08-19 2000-11-14 Isis Pharmaceuticals, Inc. 2'-O-acetamido modified monomers and oligomers
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
AU2001241621B2 (en) 2000-02-23 2005-08-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination
US7179590B2 (en) 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
MY164523A (en) 2000-05-23 2017-12-29 Univ Degli Studi Cagliari Methods and compositions for treating hepatitis c virus
FR2810322B1 (fr) 2000-06-14 2006-11-10 Pasteur Institut PRODUCTION COMBINATOIRE D'ANALOGUES DE NUCLEOTIDES ET NUCLEOTIDES (XiTP)
WO2002057425A2 (en) 2001-01-22 2002-07-25 Merck & Co., Inc. Nucleoside derivatives as inhibitors of rna-dependent rna viral polymerase
EP1409731A2 (en) 2001-07-03 2004-04-21 ISIS Pharmaceuticals, Inc. Methods of modulating pharmacokinetics of oligonucleotides
US6921812B1 (en) 2001-07-03 2005-07-26 Isis Pharmaceuticals, Inc. Methods of modulating pharmacokinetics of oligonucleotides
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
EP1509619B1 (en) * 2002-05-10 2012-04-04 City of Hope Pyrophosphorolysis activated polymerization (pap)
JP3827629B2 (ja) 2002-08-30 2006-09-27 住友大阪セメント株式会社 光変調器
AU2003294716A1 (en) 2002-11-22 2004-06-18 F.Hoffmann-La Roche Ag Detectable labeled nucleoside analogs and methods of use thereof
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
AU2005250239A1 (en) 2004-06-02 2005-12-15 Alere San Diego, Inc. 2'-nitrobenzyl-modified ribonucleotides
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
JP4753066B2 (ja) 2005-06-29 2011-08-17 独立行政法人産業技術総合研究所 イオン複合型粘土組成物およびその固化物
WO2007039301A2 (en) 2005-10-05 2007-04-12 Roche Diagnostics Gmbh Non-fluorescent energy transfer
CN101346472B (zh) 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型

Also Published As

Publication number Publication date
ATE542922T1 (de) 2012-02-15
JP2010506573A (ja) 2010-03-04
EP2076606B1 (en) 2012-01-25
CA2664229C (en) 2012-12-18
JP5421112B2 (ja) 2014-02-19
US20070154914A1 (en) 2007-07-05
EP2076606A2 (en) 2009-07-08
US7745125B2 (en) 2010-06-29
WO2008046602A2 (en) 2008-04-24
CA2664229A1 (en) 2008-04-24
CN101528945A (zh) 2009-09-09
CN105274169A (zh) 2016-01-27
ES2381505T3 (es) 2012-05-28
WO2008046602A3 (en) 2008-06-26

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