ES2381505T3 - Polimerización activada por pirofosforólisis relativa a un extremo en 2' - Google Patents

Polimerización activada por pirofosforólisis relativa a un extremo en 2' Download PDF

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Publication number
ES2381505T3
ES2381505T3 ES07819065T ES07819065T ES2381505T3 ES 2381505 T3 ES2381505 T3 ES 2381505T3 ES 07819065 T ES07819065 T ES 07819065T ES 07819065 T ES07819065 T ES 07819065T ES 2381505 T3 ES2381505 T3 ES 2381505T3
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ES
Spain
Prior art keywords
polymerase
nucleotide
nucleic acid
group
oligonucleotide
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Active
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ES07819065T
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English (en)
Spanish (es)
Inventor
David Harrow Gelfand
Keith A. Bauer
Amar P. Gupta
Veeraiah Bodepudi
John Niemiec
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F Hoffmann La Roche AG
Roche Diagnostics GmbH
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F Hoffmann La Roche AG
Roche Diagnostics GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
ES07819065T 2006-10-18 2007-10-17 Polimerización activada por pirofosforólisis relativa a un extremo en 2' Active ES2381505T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US583606 2006-10-18
US11/583,606 US7745125B2 (en) 2004-06-28 2006-10-18 2′-terminator related pyrophosphorolysis activated polymerization
PCT/EP2007/008997 WO2008046602A2 (en) 2006-10-18 2007-10-17 2'-terminator related pyrophosphorolysis activated polymerization

Publications (1)

Publication Number Publication Date
ES2381505T3 true ES2381505T3 (es) 2012-05-28

Family

ID=39283915

Family Applications (1)

Application Number Title Priority Date Filing Date
ES07819065T Active ES2381505T3 (es) 2006-10-18 2007-10-17 Polimerización activada por pirofosforólisis relativa a un extremo en 2'

Country Status (9)

Country Link
US (1) US7745125B2 (https=)
EP (1) EP2076606B1 (https=)
JP (1) JP5421112B2 (https=)
CN (2) CN105274169A (https=)
AT (1) ATE542922T1 (https=)
CA (1) CA2664229C (https=)
ES (1) ES2381505T3 (https=)
HK (1) HK1220733A1 (https=)
WO (1) WO2008046602A2 (https=)

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US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
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CN104862401B (zh) * 2015-05-29 2017-10-31 沈阳优吉诺生物科技有限公司 检测kras基因热点突变位点的引物、试剂盒及其pcr方法
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
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Also Published As

Publication number Publication date
CN105274169A (zh) 2016-01-27
JP2010506573A (ja) 2010-03-04
EP2076606B1 (en) 2012-01-25
WO2008046602A2 (en) 2008-04-24
ATE542922T1 (de) 2012-02-15
JP5421112B2 (ja) 2014-02-19
HK1220733A1 (zh) 2017-05-12
CN101528945A (zh) 2009-09-09
EP2076606A2 (en) 2009-07-08
WO2008046602A3 (en) 2008-06-26
CA2664229A1 (en) 2008-04-24
US7745125B2 (en) 2010-06-29
US20070154914A1 (en) 2007-07-05
CA2664229C (en) 2012-12-18

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