JP5421112B2 - ２’−終止関連の加ピロリン酸分解活性化された重合 - Google Patents

２’−終止関連の加ピロリン酸分解活性化された重合 Download PDF

Info

Publication number
JP5421112B2
JP5421112B2 JP2009532720A JP2009532720A JP5421112B2 JP 5421112 B2 JP5421112 B2 JP 5421112B2 JP 2009532720 A JP2009532720 A JP 2009532720A JP 2009532720 A JP2009532720 A JP 2009532720A JP 5421112 B2 JP5421112 B2 JP 5421112B2
Authority
JP
Japan
Prior art keywords
nucleic acid
polymerase
nucleotide
group
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2009532720A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010506573A (ja
JP2010506573A5 (https=
Inventor
ハロウ ゲルファンド，デイビッド
エー． バウアー，キース
ピー． グプタ，エイマー
ボードプディ，ビーライアー
ニーミーク，ジョン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2010506573A publication Critical patent/JP2010506573A/ja
Publication of JP2010506573A5 publication Critical patent/JP2010506573A5/ja
Application granted granted Critical
Publication of JP5421112B2 publication Critical patent/JP5421112B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
JP2009532720A 2006-10-18 2007-10-17 ２’−終止関連の加ピロリン酸分解活性化された重合 Expired - Fee Related JP5421112B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/583,606 2006-10-18
US11/583,606 US7745125B2 (en) 2004-06-28 2006-10-18 2′-terminator related pyrophosphorolysis activated polymerization
PCT/EP2007/008997 WO2008046602A2 (en) 2006-10-18 2007-10-17 2'-terminator related pyrophosphorolysis activated polymerization

Publications (3)

Publication Number Publication Date
JP2010506573A JP2010506573A (ja) 2010-03-04
JP2010506573A5 JP2010506573A5 (https=) 2010-09-16
JP5421112B2 true JP5421112B2 (ja) 2014-02-19

Family

ID=39283915

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009532720A Expired - Fee Related JP5421112B2 (ja) 2006-10-18 2007-10-17 ２’−終止関連の加ピロリン酸分解活性化された重合

Country Status (9)

Country Link
US (1) US7745125B2 (https=)
EP (1) EP2076606B1 (https=)
JP (1) JP5421112B2 (https=)
CN (2) CN105274169A (https=)
AT (1) ATE542922T1 (https=)
CA (1) CA2664229C (https=)
ES (1) ES2381505T3 (https=)
HK (1) HK1220733A1 (https=)
WO (1) WO2008046602A2 (https=)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7928207B2 (en) * 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
CN101346472B (zh) * 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
ES2553893T3 (es) * 2007-11-28 2015-12-14 F. Hoffmann-La Roche Ag ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP)
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
JP5847076B2 (ja) 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
ES3022638T3 (en) 2009-06-05 2025-05-28 Abbott Diagnostics Scarborough Inc Recombinase polymerase amplification reagents
JP2013501523A (ja) * 2009-08-11 2013-01-17 レスポンス ジェネティクス，インコーポレイティド Ｂｒａｆ変異の検出に有用な方法、プライマー、プローブ、及びキット
US8409802B2 (en) * 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
US8932813B2 (en) 2010-12-13 2015-01-13 Life Technologies Corporation Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions
BR112013025758B1 (pt) 2011-04-07 2021-04-06 Abbott Diagnostics Scarborough, Inc. Monitoramento de misturas de amplificação de recombinase polimerase
ES2625288T3 (es) 2011-04-15 2017-07-19 The Johns Hopkins University Sistema de secuenciación segura
MD20140044A2 (ro) 2011-11-11 2014-08-31 Pfizer Inc. 2-Tiopirimidinone şi utilizarea lor pentru tratamentul afecţiunilor cardiovasculare
KR101545848B1 (ko) * 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
AU2013338393C1 (en) 2012-10-29 2024-07-25 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US8808989B1 (en) * 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
AU2016257179A1 (en) 2015-05-05 2017-11-02 Pfizer Inc. 2-thiopyrimidinones
CN104862401B (zh) * 2015-05-29 2017-10-31 沈阳优吉诺生物科技有限公司 检测kras基因热点突变位点的引物、试剂盒及其pcr方法
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
IL319255A (en) 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
EA202091673A1 (ru) 2018-01-29 2021-02-03 Ст. Джуд Чилдрен'С Рисерч Хоспитал, Инк. Способ амплификации нуклеиновых кислот
ES2874753T3 (es) * 2018-07-19 2021-11-05 Biofidelity Ltd Método mejorado de detección de secuencia de polinucleótidos
EP4004230A4 (en) * 2019-07-31 2023-08-09 Bioskryb Genomics, Inc. GENETIC MUTATION ANALYSIS
GB201919186D0 (en) * 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
JP2023513606A (ja) 2020-02-14 2023-03-31 ザ・ジョンズ・ホプキンス・ユニバーシティー 核酸を評価するための方法および材料
US11634754B2 (en) 2021-04-15 2023-04-25 Biofidelity Ltd. Nucleic acid enrichment and detection

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL41678C (https=) 1931-07-27
US3338882A (en) 1965-06-28 1967-08-29 Upjohn Co 2'-and 3'-phosphates of 1-beta-d-arabinofuranosylcytosine
JPS5290693A (en) 1977-02-21 1977-07-30 Kyowa Hakko Kogyo Co Ltd Preparation of guanine nucleotide by fermentation
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
CA1219824A (en) 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
JPS5985298A (ja) 1982-11-04 1984-05-17 Yamasa Shoyu Co Ltd ３′−デオキシグアノシンの製造法
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
DE3803021A1 (de) 1988-02-02 1989-08-10 Geiger Reinhard Atp-derivate
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JPH0778074B2 (ja) 1989-09-29 1995-08-23 堺化学工業株式会社 ヌクレオシド―２’，５’‐及び３’，５’‐二リン酸の製造方法
ATE269870T1 (de) 1989-10-24 2004-07-15 Isis Pharmaceuticals Inc 2'-modifizierte oligonukleotide
US5914396A (en) 1990-01-11 1999-06-22 Isis Pharmaceuticals, Inc. 2'-O-modified nucleosides and phosphoramidites
US6005087A (en) 1995-06-06 1999-12-21 Isis Pharmaceuticals, Inc. 2'-modified oligonucleotides
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
AU4848793A (en) 1992-09-11 1994-04-12 Isis Pharmaceuticals, Inc. Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
CA2153387A1 (en) 1993-01-07 1994-07-21 Hubert Koester Dna sequencing by mass spectrometry
US5532130A (en) 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
US5798340A (en) 1993-09-17 1998-08-25 Gilead Sciences, Inc. Nucleotide analogs
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
ES2203635T3 (es) 1994-04-27 2004-04-16 Novartis Ag Nucleosidos y oligonucleotidos con grupos 2'-eter.
JPH09316093A (ja) 1996-03-28 1997-12-09 Sankyo Co Ltd ポリリン酸誘導体
CZ293215B6 (cs) 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
US6699668B1 (en) 1997-01-15 2004-03-02 Xzillion Gmbh & Co. Mass label linked hybridisation probes
GB9718921D0 (en) 1997-09-05 1997-11-12 Brax Genomics Ltd Catalytically generated mass labels
US20030083273A1 (en) 1999-04-20 2003-05-01 Tod M. Woolf Antisense oligomers
US6147200A (en) 1999-08-19 2000-11-14 Isis Pharmaceuticals, Inc. 2'-O-acetamido modified monomers and oligomers
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
AU2001241621B2 (en) 2000-02-23 2005-08-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination
US7179590B2 (en) 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
MY164523A (en) 2000-05-23 2017-12-29 Univ Degli Studi Cagliari Methods and compositions for treating hepatitis c virus
FR2810322B1 (fr) 2000-06-14 2006-11-10 Pasteur Institut PRODUCTION COMBINATOIRE D'ANALOGUES DE NUCLEOTIDES ET NUCLEOTIDES (XiTP)
WO2002057425A2 (en) 2001-01-22 2002-07-25 Merck & Co., Inc. Nucleoside derivatives as inhibitors of rna-dependent rna viral polymerase
EP1409731A2 (en) 2001-07-03 2004-04-21 ISIS Pharmaceuticals, Inc. Methods of modulating pharmacokinetics of oligonucleotides
US6921812B1 (en) 2001-07-03 2005-07-26 Isis Pharmaceuticals, Inc. Methods of modulating pharmacokinetics of oligonucleotides
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
EP1509619B1 (en) * 2002-05-10 2012-04-04 City of Hope Pyrophosphorolysis activated polymerization (pap)
JP3827629B2 (ja) 2002-08-30 2006-09-27 住友大阪セメント株式会社 光変調器
AU2003294716A1 (en) 2002-11-22 2004-06-18 F.Hoffmann-La Roche Ag Detectable labeled nucleoside analogs and methods of use thereof
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
AU2005250239A1 (en) 2004-06-02 2005-12-15 Alere San Diego, Inc. 2'-nitrobenzyl-modified ribonucleotides
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
JP4753066B2 (ja) 2005-06-29 2011-08-17 独立行政法人産業技術総合研究所 イオン複合型粘土組成物およびその固化物
WO2007039301A2 (en) 2005-10-05 2007-04-12 Roche Diagnostics Gmbh Non-fluorescent energy transfer
CN101346472B (zh) 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型

Also Published As

Publication number Publication date
ATE542922T1 (de) 2012-02-15
JP2010506573A (ja) 2010-03-04
EP2076606B1 (en) 2012-01-25
CA2664229C (en) 2012-12-18
US20070154914A1 (en) 2007-07-05
HK1220733A1 (zh) 2017-05-12
EP2076606A2 (en) 2009-07-08
US7745125B2 (en) 2010-06-29
WO2008046602A2 (en) 2008-04-24
CA2664229A1 (en) 2008-04-24
CN101528945A (zh) 2009-09-09
CN105274169A (zh) 2016-01-27
ES2381505T3 (es) 2012-05-28
WO2008046602A3 (en) 2008-06-26

Similar Documents

Publication Publication Date Title
JP5421112B2 (ja) ２’−終止関連の加ピロリン酸分解活性化された重合
CA2740913C (en) Improved allele-specific amplification
US6818420B2 (en) Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
CN102119226B (zh) 校对引物延伸
CN101506384B (zh) 专一性寡核苷酸和其在核酸扩增与检测中的用途
JP2010527580A (ja) ２’−ターミネーター・ヌクレオチドを含む核酸の合成及び組成物
JP6001674B2 (ja) 非特異的増幅を減少させるための方法及び試薬
CN104838015B (zh) 在等位基因特异性pcr中具有经修饰的磷酸酯和碱基的引物
US10150990B2 (en) Ribonucleotide tag nucleic acid detection
JP2017538429A (ja) 野生型の抑制のためのブロッキングオリゴヌクレオチドを使用する、核酸の対立遺伝子特異的増幅
HK1136606A (en) 2'-terminator related pyrophosphorolysis activated polymerization
HK1154388A (en) Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides
HK1159192B (en) Proofreading primer extension

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100729

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20100729

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130129

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130425

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20131022

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20131121

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees