JP2009528050A - Ｂｍｉ−１を用いた星状細胞の神経幹細胞への脱分化 - Google Patents

Ｂｍｉ−１を用いた星状細胞の神経幹細胞への脱分化 Download PDF

Info

Publication number
JP2009528050A
JP2009528050A JP2008557196A JP2008557196A JP2009528050A JP 2009528050 A JP2009528050 A JP 2009528050A JP 2008557196 A JP2008557196 A JP 2008557196A JP 2008557196 A JP2008557196 A JP 2008557196A JP 2009528050 A JP2009528050 A JP 2009528050A
Authority
JP
Japan
Prior art keywords
neural stem
bmi
astrocytes
protein
stem cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2008557196A
Other languages
English (en)
Japanese (ja)
Inventor
ユ，スングォン
フィ ムン，チェ
ソン ユン，ビョン
ドン キム，キ
パク，キュマン
ジュン ユ，スン
ギョン チョン，ウン
キム，ボナ
シク クァク，ソン
メン，イサク
Original Assignee
イムジェン カンパニー リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イムジェン カンパニー リミテッド filed Critical イムジェン カンパニー リミテッド
Publication of JP2009528050A publication Critical patent/JP2009528050A/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C12N2501/392—Sexual steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/08—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the nervous system

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
JP2008557196A 2006-02-27 2006-04-12 Ｂｍｉ−１を用いた星状細胞の神経幹細胞への脱分化 Pending JP2009528050A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20060019018 2006-02-27
PCT/KR2006/001350 WO2007097494A1 (en) 2006-02-27 2006-04-12 De-differentiation of astrocytes into neural stem cell using bmi-1

Publications (1)

Publication Number Publication Date
JP2009528050A true JP2009528050A (ja) 2009-08-06

Family

ID=38437522

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008557196A Pending JP2009528050A (ja) 2006-02-27 2006-04-12 Ｂｍｉ−１を用いた星状細胞の神経幹細胞への脱分化

Country Status (5)

Country Link
EP (1) EP1987148A4 (de)
JP (1) JP2009528050A (de)
KR (1) KR100844971B1 (de)
CN (1) CN101389761A (de)
WO (1) WO2007097494A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
EP2208786B1 (de) 2005-12-13 2018-08-01 Kyoto University Nukleärer Reprogrammierungsfaktor
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US9683232B2 (en) 2007-12-10 2017-06-20 Kyoto University Efficient method for nuclear reprogramming
WO2009075119A1 (ja) 2007-12-10 2009-06-18 Kyoto University 効率的な核初期化方法
CA2709566A1 (en) * 2007-12-17 2009-06-25 Gliamed, Inc. Stem-like cells and method for reprogramming adult mammalian somatic cells
CA2695522C (en) 2008-05-02 2019-01-15 Kyoto University Method of nuclear reprogramming
MX353245B (es) 2009-10-31 2018-01-08 New World Laboratories Inc Star Metodos para reprogramar células y sus usos.
US9453205B2 (en) 2009-10-31 2016-09-27 Genesis Technologies Limited Methods for reprogramming cells and uses thereof
KR101158402B1 (ko) * 2009-12-30 2012-06-22 주식회사 일진케이제이 Ｂｍｉ１ 및 Ｏｃｔ４를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
JP2011135864A (ja) 2009-12-30 2011-07-14 Korea Univ Research & Business Foundation Ｏｃｔ４及びＢｍｉ１、またはその上位調節子を用いて体細胞から胚幹細胞類似細胞への逆分化を誘導する組成物及びこれを用いた胚幹細胞類似細胞の製造方法
CN102242146B (zh) * 2010-05-10 2015-11-25 高丽大学校产学协力团 组合物和用其产生诱导全能干细胞的方法
CN106381280B (zh) 2010-05-13 2020-08-04 加州大学董事会 用于诱导人类多能干细胞的方法和组合物
CN106754728B (zh) * 2016-12-30 2020-05-15 中国人民解放军总医院 后肾间充质细胞系及其制备方法与应用
CN109674809B (zh) * 2018-12-27 2022-08-09 吉林大学 一种包括miR-124-3P的组合物及其在诱导神经元形成的药物中的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19516776A1 (de) * 1995-05-10 1996-11-14 Boehringer Ingelheim Int Chromatin-Regulatorgene
US6897061B1 (en) * 2000-06-16 2005-05-24 Spinal Cord Society Transdifferentiation of glial cells
US6919204B2 (en) * 2000-09-29 2005-07-19 Sangamo Biosciences, Inc. Modulation of gene expression using localization domains
JP2003033179A (ja) * 2001-07-05 2003-02-04 Asahi Kasei Corp 可逆的遺伝子導入ベクター
KR20010099203A (ko) * 2001-09-11 2001-11-09 이상훈 신경 줄기세포를 도파민성 신경세포로 분화시키는 방법

Also Published As

Publication number Publication date
EP1987148A1 (de) 2008-11-05
KR20070089089A (ko) 2007-08-30
WO2007097494A1 (en) 2007-08-30
CN101389761A (zh) 2009-03-18
EP1987148A4 (de) 2009-08-05
KR100844971B1 (ko) 2008-07-09

Similar Documents

Publication Publication Date Title
JP2009528050A (ja) Ｂｍｉ−１を用いた星状細胞の神経幹細胞への脱分化
US9249391B2 (en) Methods of generating neural stem cells
AU2014250190B2 (en) Method for producing reprogrammed derivative neuronal stem cell from non-neuronal cell by using HMGA2
US8772031B2 (en) Composition for reprogramming somatic cells to generate induced pluripotent stem cells, comprising Oct4 in combination with Bmi1 or its upstream regulator, and method for generating induced pluripotent stem cells using the same
KR100794362B1 (ko) Ｎａｎｏｇ를 이용한 아스트로사이트의 신경줄기세포로의역분화
KR100794359B1 (ko) Ｓｈｈ를 이용한 아스트로사이트의 신경줄기세포로의역분화
KR101211624B1 (ko) Ｓｈｈ, ＦＧＦＲ 티로신 키나아제 억제제, ＭＥＫ 억제제와 ＧＳＫ 억제제를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
KR101211610B1 (ko) Ｂｍｉ１, ＭＥＫ 억제제와 ＧＳＫ 억제제를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
Lubetzki et al. Clonal segregation of oligodendrocytes and astrocytes during in vitro differentiation of glial progenitor cells
KR101188878B1 (ko) 퍼모파민 및 Ｏｃｔ４를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
KR101158402B1 (ko) Ｂｍｉ１ 및 Ｏｃｔ４를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
KR101211962B1 (ko) Ｂｍｉ１, Ｇ９ａＨＭＴａｓｅ 억제제와 ＤＭＮＴ 억제제를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
KR101188877B1 (ko) 옥시스테롤 및 Ｏｃｔ４를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
KR101145376B1 (ko) Ｂｍｉ１ 유전자의 도입에 의한 리프로그래밍의 과정을 통한 상배엽 줄기세포 유사세포 제조방법 및 그 방법에 의해 제조된 역분화 줄기세포
KR101188876B1 (ko) Ｓｈｈ 및 Ｏｃｔ４를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법
KR20110124103A (ko) Ｂｍｉ１ 및 히스톤 디아세틸라아제 억제제를 이용하여 체세포로부터 배아줄기세포 유사세포로의 역분화를 유도하는 조성물 및 이를 이용한 배아줄기세포 유사세포의 제조방법

Legal Events

Date Code Title Description
A072 Dismissal of procedure [no reply to invitation to correct request for examination]

Free format text: JAPANESE INTERMEDIATE CODE: A073

Effective date: 20090728