JP2009190980A - 内臓脂肪蓄積抑制剤 - Google Patents
内臓脂肪蓄積抑制剤 Download PDFInfo
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- JP2009190980A JP2009190980A JP2008030220A JP2008030220A JP2009190980A JP 2009190980 A JP2009190980 A JP 2009190980A JP 2008030220 A JP2008030220 A JP 2008030220A JP 2008030220 A JP2008030220 A JP 2008030220A JP 2009190980 A JP2009190980 A JP 2009190980A
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- casein
- visceral fat
- fat accumulation
- accumulation inhibitor
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Abstract
【解決手段】κ-カゼインを有効成分とする内臓脂肪蓄積抑制剤、内臓脂肪蓄積抑制作用を賦与した飲食品又は飼料。
【選択図】なし
Description
また、κ-カゼインの機能性としては、κ-カゼインの部分配列であるグリコマクロペプチド(GMP)がコレシストキニンの分泌を促進させることにより食欲低下を招き、体重超過を防ぐという報告がなされている(例えば、特許文献3参照)。さらには、κ-カゼイン及びその加水分解物が、マクロファージの変性LDLとり込みを阻害するといった報告がされている(例えば、特許文献4参照)。
(1)κ-カゼインを有効成分とする内臓脂肪蓄積抑制剤。
(2)(1)記載の内臓脂肪蓄積抑制剤を配合した飲食品。
(3)(1)記載の内臓脂肪蓄積抑制剤を配合した飼料。
本発明において有効成分として使用されるκ-カゼインは、市販のκ-カゼイン、哺乳動物であるヒト、ウシ、ヤギ、ヒツジ等の乳から常套の手段により得られるもの、又は遺伝子組替え技術等によって得られるもの等を用いることができる。例えば、尿素と酸の存在下でκ-カゼインを分離する方法(Zittle, C. A. et al. J. Dairy Sci. 42,1897(1959) )等によりκ-カゼインを得ることができる。なお、κ-カゼインをタンパク質当たり10%以上含む濃縮物や、加水分解されたκ-カゼインを含んでいても良い。κ-カゼイン濃縮物は、イオン強度が0.02以下のpH7.0付近の緩衝液に溶解したカゼイン溶液を、ゲルろ過もしくは限外ろ過(10℃以下)を行うことにより分画する方法(特開昭59-91848号公報)等により得ることができる。
(脂肪細胞を用いた脂肪蓄積抑制作用の確認)
実施例1で得られたκ-カゼインを使用して、脂肪細胞に対する脂肪蓄積抑制作用を確認した。細胞はマウス由来前駆脂肪細胞である3T3-L1を用いた。3T3-L1をコンフルエントになるまで培養した後に、分化誘導としてIBMX、DEXを培地に添加し、さらにサンプルとして実施例1で得られたκ-カゼインを1mg/mlの濃度になるように添加した。その後2日ごとに培地交換を行い、インスリン、サンプル添加を行い、10日目に細胞中のトリグリセリド量を評価し(トリグリセリドE-テストワコー:和光純薬社製)、脂肪蓄積に対する作用を評価した。
(動物実験による内臓脂肪蓄積抑制作用の確認)
実施例1で得られたκ-カゼインについて、動物実験により内臓脂肪蓄積抑制作用を確認した。動物実験は4週齢雄のウィスター系ラットを1群8匹とし、κ-カゼイン未添加の高脂肪飼料を与えたA群(コントロール群)、κ-カゼインを0.2%配合した高脂肪飼料を与えたB群、κ-カゼインを1%配合した高脂肪飼料を与えたC群、κ-カゼインを5%配合した高脂肪飼料を与えたD群の4試験群に分け、1ヶ月間飼育した。各群で用いた飼料配合は表1に示す。給餌方法は、各群で摂食量がずれないように、制限給餌を行った。
これは、そのまま本発明の内臓脂肪蓄積抑制剤として使用しうる。
Claims (3)
- κ-カゼインを有効成分とする内臓脂肪蓄積抑制剤。
- 請求項1記載の内臓脂肪蓄積抑制剤を配合した飲食品。
- 請求項1記載の内臓脂肪蓄積抑制剤を配合した飼料。
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JP2008030220A JP5344669B2 (ja) | 2008-02-12 | 2008-02-12 | 内臓脂肪蓄積抑制剤 |
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JPH03220130A (ja) * | 1990-01-25 | 1991-09-27 | Snow Brand Milk Prod Co Ltd | 病原菌付着阻止剤及びそれを含有する飲食品等 |
JP2004521650A (ja) * | 2001-07-06 | 2004-07-22 | ハナー リサーチ インスティテュート | ミルクおよびカゼイン塩からカゼイン分画を抽出する方法、および新規生成物の製造方法 |
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JPH03220130A (ja) * | 1990-01-25 | 1991-09-27 | Snow Brand Milk Prod Co Ltd | 病原菌付着阻止剤及びそれを含有する飲食品等 |
JP2004521650A (ja) * | 2001-07-06 | 2004-07-22 | ハナー リサーチ インスティテュート | ミルクおよびカゼイン塩からカゼイン分画を抽出する方法、および新規生成物の製造方法 |
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JPN6013025671; Royle,P.J. et al.: 'Whey protein isolate and glycomacropeptide decrease weight gain and alter body composition in male W' British Journal of Nutrition Vol.100,No.1, 20071206, P.88-93 * |
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