JP2008539446A - サイトメータ細胞計数及びサイズ測定システム - Google Patents
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Abstract
【選択図】図3
Description
本発明は、2005年4月29日に出願された米国仮特許出願第60/676,403号の利益を主張する。2005年4月29日に出願された米国仮特許出願第60/676,403号は、参照により本明細書に援用される。
本発明を使用して、サイトメータチャネルにおいて光学検知を使用して細胞(たとえばRBC、PLT等)を計数して識別し、細胞サイズ(径、体積)を測定することができる。レーザ源(又は他の放射源)を、細長い線源として、或いは、2つの別個のスポット源として、サイトメータ又はフローチャネル内に集束させてもよい。細胞を、集束光を通ってサイトメータチャネルに流れるようにしてもよい。高品質集光光学系を使用して、細胞の鮮鋭像を形成し、長手方向軸がサイトメータチャネルの流れ方向に対して垂直に配置されることが好ましい1つ、2つ又はそれより多くの平行スリットを含む、マスク、プレート、又は不透明スクリーン上に照明を集束させてもよい。スリット間の距離は、たとえばおよそサイトメータチャネルに予期される平均細胞離隔距離であってもよい。スリットを含む不透明スクリーンを、1つ又は複数の検出器の正面に配置してもよい。細胞の像がスリットを横断する際、スリットに入射する光が部分的に遮られ、検出器における信号の低減をもたらす可能性があり、それにより幅が細胞径に比例するパルス波形が生成される。2つの隔置されたスリットが提供される場合、2つの波形により細胞流速及び細胞サイズの計算が可能になり得る。高い信号対雑音比を得ることができ、これにより、事象の容易な計数及び複数の細胞事象の識別が可能になる。パルス幅及び振幅により、さらに細胞タイプの識別が可能になり得る。
Cext−Csca(開口数)
に等しい可能性がある。図4bのグラフは、それぞれ4.5ミクロン及び5.5ミクロンの径を有する赤血球を表す検出器パルス61及び62を示す。2ミクロン径を有する血小板のパルスは、パルス61より大幅に小さい。図5は、さまざまな細胞タイプ、すなわち血小板(PBT)、赤血球(RBC)及びビーズに対する、ミクロン寸法での細胞径、細胞断面積及びCext−Csca(NA=0.28)を含む散乱パラメータの表を示す。Cext−Cscaは、相対パルス振幅を推定するための基礎となり得るものである。
フォーカス感度に関し、約10ミクロンの焦点変化により、細胞又は粒子径に約0.5ミクロン誤差、すなわち径に約10%誤差又は容積に約30%誤差がもたらされる可能性がある。較正は、カード毎に大幅に変化する可能性がある。たとえば、較正係数(C)を血液分析の開始時に導入される5.43ミクロンビーズを用いて計算し、それを試料の後の血球に適用してもよい。したがって、たとえば精密ビーズを使用して現場で較正係数を確定する手段が必要な場合もある。
本発明を少なくとも1つの例示的な例に関して説明したが、当業者には、本明細書を読むことにより多くの変形及び変更が明らかとなろう。したがって、添付の特許請求の範囲は、こうした変形及び変更をすべて包含するように従来技術に鑑みて可能な限り広く解釈されるべきものである。
Claims (10)
- 粒子分析システムであって、
チャネルと、
前記チャネルに近接する投影機構と、
前記チャネルに近接するスリット開口を有するスクリーンと、
前記スリット開口に近接する検出器と
を具備する、粒子分析システム。 - 請求項1に記載の粒子分析システムにおいて、
前記チャネルは、血液試料の粒子を搬送するためのものであり、
前記投影機構は、前記チャネルにおける前記粒子の像を前記スクリーンに表示するためのものであり、
前記検出器は、前記スリット開口を通過する前記像の一部を検出するためのものであり、
前記検出器は、前記スリット開口を通過する前記粒子の前記像を検出すると信号を出力する、粒子分析システム。 - 請求項2に記載の粒子分析システムにおいて、
前記信号の幅は、前記粒子の径に関する情報を提供し、
前記信号の振幅は、種類の異なる粒子を識別するための情報を提供し、且つ/又は
前記信号の前記振幅は、前記血液試料における前記粒子の数を提供する、粒子分析システム。 - 請求項3に記載の粒子分析システムにおいて、
前記粒子は赤血球及び血小板であり、
前記数は赤血球の数及び血小板の数を含み、
前記数の赤血球の該赤血球の径が概略確定され、
前記数の赤血球の平均細胞体積が概略確定され、
前記数の赤血球の赤血球分布幅が概略確定され、且つ/又は
前記赤血球の前記数と前記赤血球分布幅との積から赤血球容積率が概略確定される、粒子分析システム。 - 請求項4に記載の粒子分析システムであって、
前記血液試料におけるヘモグロビンの量を概略確定するヘモグロビンモジュールをさらに具備し、
前記ヘモグロビンの量を前記赤血球の数によって割ることにより平均細胞ヘモグロビンが概略確定され、且つ/又は
前記赤血球の数を前記赤血球容積率によって割ることにより平均細胞ヘモグロビン濃度が概略確定される、粒子分析システム。 - 粒子パラメータを確定する方法であって、
流体試料の粒子をフローチャネル内で単一列内になるように流体力学的に集束させるステップ、
前記フローチャネル内に光を投影するステップ、
前記フローチャネル内の前記粒子の像を多重スリット開口上に集束させるステップ、
前記開口からの光の遮りを検出するステップ、
前記光の遮りを、該光の遮りを表す波形に変換するステップ、及び
前記波形を前記粒子に関するデータに処理するステップ
を含む、粒子パラメータを確定する方法。 - 請求項6に記載の粒子パラメータを確定する方法において、
前記粒子に関する前記データは、
流速、
測定時間、
前記粒子の数、
時間でのパルス幅、
パルス振幅、及び/又は
粒子遷移時間
を含む、粒子パラメータを確定する方法。 - 請求項7に記載の粒子パラメータを確定する方法であって、さらに、
前記粒子のヒストグラムを作成するステップであって、それにより前記流体試料における第1のタイプの粒子の数と第2のタイプの粒子の数を得る、ステップ、
容積当りの前記第1のタイプの粒子数を計算するステップ、
容積当りの前記第2のタイプの粒子数を計算するステップ、
前記第1のタイプの粒子の平均粒子体積(MCV)を計算するステップ、及び
前記第1のタイプの粒子の分布幅(RDW)を計算するステップ
を含む、粒子パラメータを確定する方法。 - 粒子パラメータ確定システムであって、
フローチャネルと、
前記フローチャネルの第1の側に位置する光源と、
前記フローチャネルの第2の側に位置する検出器と、
前記フローチャネルと前記検出器との間に位置する開口を有するマスクと、
前記フローチャネルにおける粒子の像を前記マスクに投影する光学系構成と
を具備する、粒子パラメータ確定システム。 - 請求項9に記載の粒子パラメータ確定システムにおいて、
前記マスクは第1のスリット開口と第2のスリット開口とを備え、
前記粒子の前記像は、該粒子の該像が前記第1のスリット開口及び第2のスリット開口を横断する際に前記検出器への光を遮り、
前記検出器は、前記第1のスリット開口を横断する前記粒子の前記像を表す第1の信号と、前記第2のスリット開口を横断する前記粒子の前記像を表す第2の信号とを出力し、
前記フローチャネルにおける粒子の像の前記信号からの情報は、
第1のタイプの粒子の数、
第2のタイプの粒子の数、
少なくとも1つのタイプの粒子の平均粒子体積、及び/又は
少なくとも1つのタイプの粒子の粒子分布幅
を提供する、粒子パラメータ確定システム。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US67640305P | 2005-04-29 | 2005-04-29 | |
US60/676,403 | 2005-04-29 | ||
PCT/US2006/016440 WO2006119106A1 (en) | 2005-04-29 | 2006-04-28 | Cytometer cell counting and size measurement method |
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JP2008539446A true JP2008539446A (ja) | 2008-11-13 |
JP4965561B2 JP4965561B2 (ja) | 2012-07-04 |
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JP2008509209A Active JP4965561B2 (ja) | 2005-04-29 | 2006-04-28 | サイトメータ細胞計数及びサイズ測定システム |
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US (1) | US7688427B2 (ja) |
EP (1) | EP1875200A1 (ja) |
JP (1) | JP4965561B2 (ja) |
CN (1) | CN101438143B (ja) |
WO (1) | WO2006119106A1 (ja) |
Cited By (1)
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JP2012181124A (ja) * | 2011-03-02 | 2012-09-20 | New Industry Research Organization | 液体中の粒子のサイズの検出方法および装置 |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5966673A (en) * | 1997-01-10 | 1999-10-12 | Diamond Technologies, Inc. | System and method for computerized evaluation of gemstones |
CN102317775B (zh) * | 2009-02-16 | 2013-12-25 | 柯尼卡美能达精密光学株式会社 | 血液检查装置 |
ES2945686T3 (es) | 2009-05-13 | 2023-07-05 | Sartorius Bioanalytical Instr Inc | Medición y control de flujo para cuantificación mejorada de partículas en citometría de flujo |
JP5304456B2 (ja) * | 2009-06-10 | 2013-10-02 | ソニー株式会社 | 微小粒子測定装置 |
FR2947626B1 (fr) * | 2009-07-03 | 2012-05-11 | Centre Nat Rech Scient | Dispositif de mesure pour la caracterisation d'ecoulements diphasiques. |
US8573404B2 (en) * | 2009-12-17 | 2013-11-05 | Malvern Instruments, Ltd. | Continuous particle and macro-molecular zeta potential measurements using field flow fractionation combined micro-electrophoresis |
US8101065B2 (en) * | 2009-12-30 | 2012-01-24 | Lifescan, Inc. | Systems, devices, and methods for improving accuracy of biosensors using fill time |
US8877034B2 (en) | 2009-12-30 | 2014-11-04 | Lifescan, Inc. | Systems, devices, and methods for measuring whole blood hematocrit based on initial fill velocity |
US9057676B2 (en) | 2010-02-05 | 2015-06-16 | Cytonome/St, Llc | Multiple flow channel particle analysis system |
KR20140050606A (ko) * | 2011-05-13 | 2014-04-29 | 베크만 컬터, 인코포레이티드 | 혈액 기생충을 진단하기 위한 방법 및 장치 |
TWI708052B (zh) | 2011-08-29 | 2020-10-21 | 美商安美基公司 | 用於非破壞性檢測-流體中未溶解粒子之方法及裝置 |
EP2864760B1 (en) * | 2012-06-22 | 2024-02-28 | Malvern Panalytical Limited | Heterogeneous fluid sample characterization |
US10509976B2 (en) | 2012-06-22 | 2019-12-17 | Malvern Panalytical Limited | Heterogeneous fluid sample characterization |
US9709556B2 (en) | 2013-12-11 | 2017-07-18 | Analiza, Inc. | Devices and methods for determining and/or isolating circulating cancer cells |
US20150160246A1 (en) | 2013-12-11 | 2015-06-11 | Analiza, Inc. | Devices and methods for determining and/or isolating cells such as circulating cancer or fetal cells |
US10324020B2 (en) | 2013-12-23 | 2019-06-18 | Palo Alto Research Center Incorporated | Fluidic optical cartridge |
US9261452B2 (en) * | 2013-12-23 | 2016-02-16 | Palo Alto Research Center Incorporated | Flow cytometer |
US9207066B2 (en) | 2014-02-14 | 2015-12-08 | Palo Alto Research Center Incorporated | Spatial modulation of light to determine dimensional characteristics of objects in a flow path |
US9952033B2 (en) | 2014-02-14 | 2018-04-24 | Palo Alto Research Center Incorporated | Spatial modulation of light to determine object length |
US9528925B2 (en) | 2014-02-14 | 2016-12-27 | Palo Alto Research Center Incorporated | Spatial modulation of light to determine object position |
US10451482B2 (en) | 2014-02-14 | 2019-10-22 | Palo Alto Research Center Incorporated | Determination of color characteristics of objects using spatially modulated light |
JP6237417B2 (ja) * | 2014-03-31 | 2017-11-29 | 株式会社Jvcケンウッド | 分析装置及び分析方法 |
US9114606B1 (en) | 2014-04-07 | 2015-08-25 | Palo Alto Research Center Incorporated | Spatial light modulation method for determining droplet motion characteristics |
US9400174B2 (en) | 2014-04-07 | 2016-07-26 | Palo Alto Research Center Incorporated | Monitor for particle injector |
US10598585B2 (en) * | 2014-07-08 | 2020-03-24 | East China University Of Science And Technology | Synchronous high-speed photographing method and device for microparticle rotation in liquid cyclone field |
CN106018200A (zh) * | 2016-05-21 | 2016-10-12 | 深圳市青核桃科技有限公司 | 一种提高激光颗粒计数器测量结果精度的方法 |
US9704239B1 (en) | 2016-09-02 | 2017-07-11 | Amgen Inc. | Video trigger synchronization for improved particle detection in a vessel |
CN106404637B (zh) * | 2016-10-08 | 2018-11-13 | 重庆医科大学附属永川医院 | 一种医学检测用血细胞分析仪 |
CN106845622B (zh) * | 2017-01-22 | 2019-04-23 | 江西特康科技有限公司 | 血小板计数方法及系统 |
US10088660B2 (en) | 2017-02-10 | 2018-10-02 | Amgen Inc. | Imaging system for counting and sizing particles in fluid-filled vessels |
CN107907471A (zh) * | 2017-10-26 | 2018-04-13 | 刘峰 | 一种细胞检验计数装置 |
US10720755B2 (en) * | 2018-02-07 | 2020-07-21 | Elfi-Tech Ltd. | Ensemble-averaged measurement of stochastic motion by current-modulating of VCSEL wavelength |
US11137337B2 (en) | 2019-01-21 | 2021-10-05 | Essen Instruments, Inc. | Flow cytometry with data analysis for optimized dilution of fluid samples for flow cytometry investigation |
US11709116B2 (en) | 2020-02-04 | 2023-07-25 | Sartorius Bioanalytical Instruments, Inc. | Liquid flourescent dye concentrate for flow cytometry evaluation of virus-size particles and related products and methods |
US11852577B2 (en) * | 2021-09-29 | 2023-12-26 | Orange Biomed Ltd., Co. | Apparatus for measuring properties of particles in a solution and related methods |
US20240175799A1 (en) * | 2022-11-30 | 2024-05-30 | Eaton Intelligent Power Limited | Systems and methods for optical particle detection |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63100351A (ja) * | 1986-10-17 | 1988-05-02 | Hitachi Ltd | 粒子計数装置 |
JPH02118436A (ja) * | 1988-10-28 | 1990-05-02 | Hitachi Ltd | 粒子判別方法および細胞判別装置並びに細胞融合装置 |
Family Cites Families (111)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3390605A (en) * | 1963-10-23 | 1968-07-02 | Yanagimoto Seisakusho Co Ltd | Device for measuring simultaneously both rotatory polarization and light absorption |
US3657537A (en) | 1970-04-03 | 1972-04-18 | Bausch & Lomb | Computerized slit-scan cyto-fluorometer for automated cell recognition |
US3822095A (en) | 1972-08-14 | 1974-07-02 | Block Engineering | System for differentiating particles |
US3928094A (en) | 1975-01-16 | 1975-12-23 | Fairchild Camera Instr Co | Method of aligning a wafer beneath a mask and system therefor and wafer having a unique alignment pattern |
US3976862A (en) | 1975-03-18 | 1976-08-24 | Block Engineering, Inc. | Flow stream processor |
US4577964A (en) * | 1978-09-06 | 1986-03-25 | Ortho Diagnostics, Inc. | Apparatus and method for detecting platelets in whole blood |
US4293221A (en) | 1979-04-17 | 1981-10-06 | Research Corporation | Multidimensional slit-scan flow system |
US4284412A (en) | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
US4350892A (en) | 1980-07-31 | 1982-09-21 | Research Corporation | X'-, Y'-, Z'- axis multidimensional slit-scan flow system |
US4412004A (en) | 1981-06-26 | 1983-10-25 | Technicon Instruments Corporation | Method for treating red blood cells to effect sphering and reagent therefor |
JPS58143206A (ja) | 1982-02-19 | 1983-08-25 | Canon Inc | 位置検知信号処理装置 |
US4441816A (en) * | 1982-03-25 | 1984-04-10 | The United States Of America As Represented By The United States Department Of Energy | Optical double-slit particle measuring system |
US4478076A (en) | 1982-09-30 | 1984-10-23 | Honeywell Inc. | Flow sensor |
US4478077A (en) | 1982-09-30 | 1984-10-23 | Honeywell Inc. | Flow sensor |
US4501144A (en) | 1982-09-30 | 1985-02-26 | Honeywell Inc. | Flow sensor |
US4683159A (en) | 1982-09-30 | 1987-07-28 | Honeywell Inc. | Semiconductor device structure and processing |
US4651564A (en) | 1982-09-30 | 1987-03-24 | Honeywell Inc. | Semiconductor device |
JPS6166947A (ja) | 1984-09-10 | 1986-04-05 | Canon Inc | 粒子解析装置 |
US4695034A (en) | 1984-11-27 | 1987-09-22 | Stec Inc. | Fluid control device |
US4745279A (en) | 1986-01-02 | 1988-05-17 | American Hospital Supply Corporation | Hematocrit measuring apparatus |
US4704033A (en) | 1986-03-06 | 1987-11-03 | Micronix Corporation | Multiple wavelength linear zone plate alignment apparatus and method |
NL8601000A (nl) | 1986-04-21 | 1987-11-16 | Jan Greve T H Twente Afdeling | Het gebruik van gepolariseerd licht in stromingscytometrie. |
DE3640616A1 (de) | 1986-11-27 | 1988-06-09 | Standard Elektrik Lorenz Ag | Justiervorrichtung |
US4818263A (en) | 1987-06-11 | 1989-04-04 | Tektronix, Inc. | Method and apparatus for precisely positioning microlenses on optical fibers |
US4874949A (en) | 1987-09-14 | 1989-10-17 | Vanderbilt University | Method of measuring lung vascular function and transcapillary transport by the use of nonradioactive markers |
US4911616A (en) | 1988-01-19 | 1990-03-27 | Laumann Jr Carl W | Micro miniature implantable pump |
US5760900A (en) * | 1989-03-18 | 1998-06-02 | Canon Kabushiki Kaisha | Method and apparatus for optically measuring specimen |
US4932989A (en) | 1989-04-05 | 1990-06-12 | At&T Bell Laboratories | Method and apparatus for fabricating microlenses on optical fibers |
CH679555A5 (ja) | 1989-04-11 | 1992-03-13 | Westonbridge Int Ltd | |
CA2016699C (en) | 1989-05-15 | 2003-11-18 | Paul N. Marshall | Lytic agents and uses thereof |
ES2061042T3 (es) | 1989-06-14 | 1994-12-01 | Westonbridge Int Ltd | Microbomba perfeccionada. |
DE3926066A1 (de) | 1989-08-07 | 1991-02-14 | Ibm Deutschland | Mikromechanische kompressorkaskade und verfahren zur druckerhoehung bei extrem niedrigem arbeitsdruck |
CH681168A5 (en) | 1989-11-10 | 1993-01-29 | Westonbridge Int Ltd | Micro-pump for medicinal dosing |
US5244537A (en) | 1989-12-27 | 1993-09-14 | Honeywell, Inc. | Fabrication of an electronic microvalve apparatus |
US5082242A (en) | 1989-12-27 | 1992-01-21 | Ulrich Bonne | Electronic microvalve apparatus and fabrication |
US5171132A (en) | 1989-12-27 | 1992-12-15 | Seiko Epson Corporation | Two-valve thin plate micropump |
US5050429A (en) | 1990-02-22 | 1991-09-24 | Yamatake-Honeywell Co., Ltd. | Microbridge flow sensor |
US5096388A (en) | 1990-03-22 | 1992-03-17 | The Charles Stark Draper Laboratory, Inc. | Microfabricated pump |
US5159642A (en) * | 1990-07-13 | 1992-10-27 | Toa Medical Electronics Co., Ltd. | Particle image analyzing apparatus |
DE69104585T2 (de) | 1990-10-30 | 1995-05-18 | Hewlett Packard Co | Mikropumpe. |
DE69129260T2 (de) | 1990-11-03 | 1998-11-19 | Horiba Ltd | Gerät zur Messung der Teilchengrössenverteilung |
US5108623A (en) | 1990-11-19 | 1992-04-28 | Gould Inc. | Moving web filter assembly |
US5194909A (en) * | 1990-12-04 | 1993-03-16 | Tycko Daniel H | Apparatus and method for measuring volume and hemoglobin concentration of red blood cells |
DE4119955C2 (de) | 1991-06-18 | 2000-05-31 | Danfoss As | Miniatur-Betätigungselement |
US5176358A (en) | 1991-08-08 | 1993-01-05 | Honeywell Inc. | Microstructure gas valve control |
EP0648351B1 (en) | 1992-07-02 | 1997-10-08 | Indigo N.V. | Concentration detector for colored toner |
JP3215175B2 (ja) | 1992-08-10 | 2001-10-02 | シスメックス株式会社 | 粒子分析装置 |
US5441597A (en) | 1992-12-01 | 1995-08-15 | Honeywell Inc. | Microstructure gas valve control forming method |
DE69432410T2 (de) | 1993-05-14 | 2004-03-04 | Coulter International Corp., Miami | Retikulozyt bestimmungsverfahren und geraet, das lichtstreuungstechniken verwendet |
JPH07120375A (ja) * | 1993-10-21 | 1995-05-12 | Hitachi Ltd | フロー式粒子画像解析方法及び装置 |
US5540494A (en) * | 1994-06-03 | 1996-07-30 | Purvis, Jr.; Norman B. | Method and apparatus for determining absolute particle size, surface area and volume normalized fluorescence using forward angle light scatter intensity in flow cytometry |
US5601080A (en) | 1994-12-28 | 1997-02-11 | Coretech Medical Technologies Corporation | Spectrophotometric blood analysis |
US5793485A (en) * | 1995-03-20 | 1998-08-11 | Sandia Corporation | Resonant-cavity apparatus for cytometry or particle analysis |
US5528045A (en) | 1995-04-06 | 1996-06-18 | Becton Dickinson And Company | Particle analyzer with spatially split wavelength filter |
AU6541596A (en) * | 1995-06-16 | 1997-01-15 | University Of Washington | Microfabricated differential extraction device and method |
US5716852A (en) | 1996-03-29 | 1998-02-10 | University Of Washington | Microfabricated diffusion-based chemical sensor |
US5922210A (en) * | 1995-06-16 | 1999-07-13 | University Of Washington | Tangential flow planar microfabricated fluid filter and method of using thereof |
US5717631A (en) | 1995-07-21 | 1998-02-10 | Carnegie Mellon University | Microelectromechanical structure and process of making same |
US5633724A (en) | 1995-08-29 | 1997-05-27 | Hewlett-Packard Company | Evanescent scanning of biochemical array |
US5726751A (en) | 1995-09-27 | 1998-03-10 | University Of Washington | Silicon microchannel optical flow cytometer |
IT1280475B1 (it) * | 1995-11-09 | 1998-01-20 | Fiat Ricerche | Dispositivi a microfiltri selettivi di colori e immagini. |
DE19546570C1 (de) * | 1995-12-13 | 1997-03-27 | Inst Mikro Und Informationstec | Fluidpumpe |
JP3308441B2 (ja) * | 1995-12-19 | 2002-07-29 | シスメックス株式会社 | 尿中有形成分分析装置 |
US5863502A (en) * | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
JP3127111B2 (ja) * | 1996-02-22 | 2001-01-22 | 株式会社日立製作所 | フロー式粒子画像解析方法および装置 |
US5948684A (en) * | 1997-03-31 | 1999-09-07 | University Of Washington | Simultaneous analyte determination and reference balancing in reference T-sensor devices |
AU3570797A (en) * | 1996-06-14 | 1998-01-07 | University Of Washington | Absorption-enhanced differential extraction device |
US5764674A (en) * | 1996-06-28 | 1998-06-09 | Honeywell Inc. | Current confinement for a vertical cavity surface emitting laser |
US5799030A (en) * | 1996-07-26 | 1998-08-25 | Honeywell Inc. | Semiconductor device with a laser and a photodetector in a common container |
AU717626B2 (en) * | 1996-10-03 | 2000-03-30 | Debiotech S.A. | Micro-machined device for fluids and method of manufacture |
US6124663A (en) * | 1996-12-16 | 2000-09-26 | The Boeing Company | Fiber optic connector having a microelectromechanical positioning apparatus and an associated fabrication method |
US5683159A (en) | 1997-01-03 | 1997-11-04 | Johnson; Greg P. | Hardware mounting rail |
US6097859A (en) | 1998-02-12 | 2000-08-01 | The Regents Of The University Of California | Multi-wavelength cross-connect optical switch |
US5974867A (en) * | 1997-06-13 | 1999-11-02 | University Of Washington | Method for determining concentration of a laminar sample stream |
WO1998059233A1 (en) * | 1997-06-23 | 1998-12-30 | Luminex Corporation | Interlaced lasers for multiple fluorescence measurement |
US6082185A (en) * | 1997-07-25 | 2000-07-04 | Research International, Inc. | Disposable fluidic circuit cards |
US5880474A (en) * | 1997-08-29 | 1999-03-09 | Becton Dickinson And Company | Multi-illumination-source flow particle analyzer with inter-location emissions crosstalk cancelation |
US6007775A (en) * | 1997-09-26 | 1999-12-28 | University Of Washington | Multiple analyte diffusion based chemical sensor |
US6106245A (en) * | 1997-10-09 | 2000-08-22 | Honeywell | Low cost, high pumping rate electrostatically actuated mesopump |
US5836750A (en) * | 1997-10-09 | 1998-11-17 | Honeywell Inc. | Electrostatically actuated mesopump having a plurality of elementary cells |
US5822170A (en) * | 1997-10-09 | 1998-10-13 | Honeywell Inc. | Hydrophobic coating for reducing humidity effect in electrostatic actuators |
US5901939A (en) * | 1997-10-09 | 1999-05-11 | Honeywell Inc. | Buckled actuator with enhanced restoring force |
CA2221324A1 (en) * | 1997-11-17 | 1999-05-17 | Eagle Precision Technologies Inc. | Tub bending apparatus and method |
US6054335A (en) * | 1997-12-12 | 2000-04-25 | Xerox Corporation | Fabrication of scanning III-V compound light emitters integrated with Si-based actuators |
US6116756A (en) * | 1997-12-12 | 2000-09-12 | Xerox Corporation | Monolithic scanning light emitting devices |
JP3522535B2 (ja) | 1998-05-29 | 2004-04-26 | 忠弘 大見 | 圧力式流量制御装置を備えたガス供給設備 |
US6091197A (en) * | 1998-06-12 | 2000-07-18 | Xerox Corporation | Full color tunable resonant cavity organic light emitting diode |
JP4001436B2 (ja) * | 1998-07-23 | 2007-10-31 | 三菱電機株式会社 | 光スイッチ及び光スイッチを用いた光路切換装置 |
DE19835070B4 (de) | 1998-08-04 | 2006-03-16 | Carl Zeiss Jena Gmbh | Anordnung zur einstellbaren wellenlängenabhängigen Detektion in einem Fluoreszenzmikroskop |
US6032689A (en) * | 1998-10-30 | 2000-03-07 | Industrial Technology Research Institute | Integrated flow controller module |
US6091537A (en) * | 1998-12-11 | 2000-07-18 | Xerox Corporation | Electro-actuated microlens assemblies |
US6215221B1 (en) * | 1998-12-29 | 2001-04-10 | Honeywell International Inc. | Electrostatic/pneumatic actuators for active surfaces |
US6184607B1 (en) * | 1998-12-29 | 2001-02-06 | Honeywell International Inc. | Driving strategy for non-parallel arrays of electrostatic actuators sharing a common electrode |
US6249341B1 (en) * | 1999-01-25 | 2001-06-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US6097485A (en) * | 1999-03-08 | 2000-08-01 | Integrated Waveguides, Inc. | Microchip optical transport technology for use in a personal flow cytometer |
US6179586B1 (en) * | 1999-09-15 | 2001-01-30 | Honeywell International Inc. | Dual diaphragm, single chamber mesopump |
US6240944B1 (en) * | 1999-09-23 | 2001-06-05 | Honeywell International Inc. | Addressable valve arrays for proportional pressure or flow control |
US6813017B1 (en) * | 1999-10-20 | 2004-11-02 | Becton, Dickinson And Company | Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer |
US6281975B1 (en) * | 2000-03-07 | 2001-08-28 | Eldex Laboratories, Inc. | Capillary flow cell with bulbous ends |
US20010030743A1 (en) | 2000-03-10 | 2001-10-18 | Carlos Araujo | Laser alignment system with plural lasers for impingement on a single target |
DE20008228U1 (de) | 2000-05-09 | 2002-01-03 | Busch Dieter & Co Prueftech | Vorrichtung zur quantitativen Beurteilung der fluchtenden Lage zweier Maschinenteile, Werkstücke o.dgl. |
US7978329B2 (en) * | 2000-08-02 | 2011-07-12 | Honeywell International Inc. | Portable scattering and fluorescence cytometer |
US7553453B2 (en) * | 2000-06-02 | 2009-06-30 | Honeywell International Inc. | Assay implementation in a microfluidic format |
US8071051B2 (en) * | 2004-05-14 | 2011-12-06 | Honeywell International Inc. | Portable sample analyzer cartridge |
US7641856B2 (en) | 2004-05-14 | 2010-01-05 | Honeywell International Inc. | Portable sample analyzer with removable cartridge |
US6549275B1 (en) * | 2000-08-02 | 2003-04-15 | Honeywell International Inc. | Optical detection system for flow cytometry |
US6597438B1 (en) * | 2000-08-02 | 2003-07-22 | Honeywell International Inc. | Portable flow cytometry |
US6382228B1 (en) * | 2000-08-02 | 2002-05-07 | Honeywell International Inc. | Fluid driving system for flow cytometry |
US6778263B2 (en) * | 2000-08-25 | 2004-08-17 | Amnis Corporation | Methods of calibrating an imaging system using calibration beads |
EP1687626A4 (en) * | 2003-06-23 | 2009-11-25 | Sewon Meditech Inc | APPARATUS FOR MEASURING THE DEFORMATION CAPACITY OF BLOOD CELLS |
US6907859B1 (en) * | 2004-05-11 | 2005-06-21 | Barnett Joel Robinson | Internal combustion engine with elevated expansion ratio |
-
2006
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63100351A (ja) * | 1986-10-17 | 1988-05-02 | Hitachi Ltd | 粒子計数装置 |
JPH02118436A (ja) * | 1988-10-28 | 1990-05-02 | Hitachi Ltd | 粒子判別方法および細胞判別装置並びに細胞融合装置 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2012181124A (ja) * | 2011-03-02 | 2012-09-20 | New Industry Research Organization | 液体中の粒子のサイズの検出方法および装置 |
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CN101438143B (zh) | 2013-06-12 |
US20060244964A1 (en) | 2006-11-02 |
CN101438143A (zh) | 2009-05-20 |
JP4965561B2 (ja) | 2012-07-04 |
US7688427B2 (en) | 2010-03-30 |
EP1875200A1 (en) | 2008-01-09 |
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