JP2008523786A - 高忠実度合成ポリヌクレオチドのアセンブリ方法 - Google Patents

高忠実度合成ポリヌクレオチドのアセンブリ方法 Download PDF

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JP2008523786A
JP2008523786A JP2007537994A JP2007537994A JP2008523786A JP 2008523786 A JP2008523786 A JP 2008523786A JP 2007537994 A JP2007537994 A JP 2007537994A JP 2007537994 A JP2007537994 A JP 2007537994A JP 2008523786 A JP2008523786 A JP 2008523786A
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polynucleotide
oligonucleotide
oligonucleotides
sequence
pool
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JP2008523786A5 (enExample
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アフェヤン ヌーバー
チャーチ ジョージ
ジェイコブスン ジョセフ
エム．ベインズ ブライアン
ガブリエル ネスミス ケネス
アラン チャップマン ブラッド
ストラック−ローグ ベティーナ
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コドン デバイシズ インコーポレイテッド
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Priority claimed from US11/068,321 external-priority patent/US20060194214A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
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  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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JP2007537994A 2004-10-18 2005-10-18 高忠実度合成ポリヌクレオチドのアセンブリ方法 Pending JP2008523786A (ja)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US61965004P 2004-10-18 2004-10-18
US64381305P 2005-01-13 2005-01-13
US65701405P 2005-02-28 2005-02-28
US11/067,812 US20070122817A1 (en) 2005-02-28 2005-02-28 Methods for assembly of high fidelity synthetic polynucleotides
US11/068,321 US20060194214A1 (en) 2005-02-28 2005-02-28 Methods for assembly of high fidelity synthetic polynucleotides
US69856005P 2005-07-12 2005-07-12
US72720505P 2005-10-14 2005-10-14
PCT/US2005/037571 WO2006044956A1 (en) 2004-10-18 2005-10-18 Methods for assembly of high fidelity synthetic polynucleotides

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JP2008523786A5 JP2008523786A5 (enExample) 2008-11-20

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EP (1) EP1812598A1 (enExample)
JP (1) JP2008523786A (enExample)
AU (1) AU2005295351A1 (enExample)
CA (1) CA2584984A1 (enExample)
WO (1) WO2006044956A1 (enExample)

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JP2014526899A (ja) * 2011-08-26 2014-10-09 ジェン９・インコーポレイテッド 核酸の高忠実度アセンブリのための組成物および方法
JP2015532643A (ja) * 2012-08-16 2015-11-12 シンセティック ジェノミクス インコーポレーテッド デジタル生物学的コンバータ
JP2016515384A (ja) * 2013-03-19 2016-05-30 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
JP2016537003A (ja) * 2013-11-18 2016-12-01 ルビコン ゲノミクス インコーポレイテッド バックグラウンド減少のための分解可能なアダプター
JP2018532705A (ja) * 2015-09-18 2018-11-08 ツイスト バイオサイエンス コーポレーション オリゴ核酸変異体ライブラリーとその合成
JP2020518247A (ja) * 2017-05-03 2020-06-25 オックスフォード ユニバーシティ イノベーション リミテッドＯｘｆｏｒｄ Ｕｎｉｖｅｒｓｉｔｙ Ｉｎｎｏｖａｔｉｏｎ Ｌｉｍｉｔｅｄ Ｄｎａアセンブリ
JP2020537511A (ja) * 2017-09-29 2020-12-24 エボネティクス リミテッド 標的二本鎖核酸のハイブリダイゼーションの間のエラー検出
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