AU2005295351A1 - Methods for assembly of high fidelity synthetic polynucleotides - Google Patents

Methods for assembly of high fidelity synthetic polynucleotides Download PDF

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Publication number
AU2005295351A1
AU2005295351A1 AU2005295351A AU2005295351A AU2005295351A1 AU 2005295351 A1 AU2005295351 A1 AU 2005295351A1 AU 2005295351 A AU2005295351 A AU 2005295351A AU 2005295351 A AU2005295351 A AU 2005295351A AU 2005295351 A1 AU2005295351 A1 AU 2005295351A1
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AU
Australia
Prior art keywords
oligonucleotides
construction
pool
construct
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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AU2005295351A
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English (en)
Inventor
Noubar Afeyan
Brian M. Baynes
Brad Alan Chapman
George Church
Joseph Jacobson
Kenneth Gabriel Nesmith
Bettina Strack-Logue
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Codon Devices Inc
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Codon Devices Inc
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Publication date
Priority claimed from US11/068,321 external-priority patent/US20060194214A1/en
Priority claimed from US11/067,812 external-priority patent/US20070122817A1/en
Application filed by Codon Devices Inc filed Critical Codon Devices Inc
Publication of AU2005295351A1 publication Critical patent/AU2005295351A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2005295351A 2004-10-18 2005-10-18 Methods for assembly of high fidelity synthetic polynucleotides Abandoned AU2005295351A1 (en)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US61965004P 2004-10-18 2004-10-18
US60/619,650 2004-10-18
US64381305P 2005-01-13 2005-01-13
US60/643,813 2005-01-13
US65701405P 2005-02-28 2005-02-28
US11/068,321 US20060194214A1 (en) 2005-02-28 2005-02-28 Methods for assembly of high fidelity synthetic polynucleotides
US11/067,812 2005-02-28
US11/067,812 US20070122817A1 (en) 2005-02-28 2005-02-28 Methods for assembly of high fidelity synthetic polynucleotides
US11/068,321 2005-02-28
US60/657,014 2005-02-28
US69856005P 2005-07-12 2005-07-12
US60/698,560 2005-07-12
US72720505P 2005-10-14 2005-10-14
US60/727,205 2005-10-14
PCT/US2005/037571 WO2006044956A1 (en) 2004-10-18 2005-10-18 Methods for assembly of high fidelity synthetic polynucleotides

Publications (1)

Publication Number Publication Date
AU2005295351A1 true AU2005295351A1 (en) 2006-04-27

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2005295351A Abandoned AU2005295351A1 (en) 2004-10-18 2005-10-18 Methods for assembly of high fidelity synthetic polynucleotides

Country Status (6)

Country Link
US (1) US20070269870A1 (enExample)
EP (1) EP1812598A1 (enExample)
JP (1) JP2008523786A (enExample)
AU (1) AU2005295351A1 (enExample)
CA (1) CA2584984A1 (enExample)
WO (1) WO2006044956A1 (enExample)

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