JP2008504841A5 - - Google Patents

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JP2008504841A5
JP2008504841A5 JP2007520407A JP2007520407A JP2008504841A5 JP 2008504841 A5 JP2008504841 A5 JP 2008504841A5 JP 2007520407 A JP2007520407 A JP 2007520407A JP 2007520407 A JP2007520407 A JP 2007520407A JP 2008504841 A5 JP2008504841 A5 JP 2008504841A5
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JP
Japan
Prior art keywords
composition
concentration
atp
divalent cation
extractant comprises
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JP2007520407A
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English (en)
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JP2008504841A (ja
JP5255273B2 (ja
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Priority claimed from PCT/US2005/023545 external-priority patent/WO2006085972A2/en
Publication of JP2008504841A publication Critical patent/JP2008504841A/ja
Publication of JP2008504841A5 publication Critical patent/JP2008504841A5/ja
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Claims (16)

  1. （ａ）反応緩衝液、
    （ｂ）第１の濃度の二価カチオン、
    （ｃ）任意成分としての第２の濃度の二価カチオンキレート剤、
    （ｄ）少なくとも１種のＡＴＰ抽出剤、及び
    （ｅ）ルシフェラーゼ酵素
    を含んでなり、
    該少なくとも１種のＡＴＰ抽出剤は、細菌又は酵母の完全性を破壊して、ＡＴＰの抽出をもたらすことができる化合物または化合物の組み合わせを含み、
    第１の濃度と第２の濃度の間の差が５ｍＭ未満である、微生物を含有することが疑われるサンプル中のＡＴＰを検出するための組成物。
  2. 第１の濃度が２．５ｍＭ未満である、請求項１に記載の組成物。
  3. 第１の濃度が１．０ｍＭ未満である、請求項１に記載の組成物。
  4. 第２の濃度の二価カチオンキレート剤を含む、請求項１に記載の組成物。
  5. 第１の濃度が少なくとも１０ｍＭである、請求項４に記載の組成物。
  6. 第１の濃度が少なくとも２０ｍＭである、請求項４に記載の組成物。
  7. 第２の濃度が第１の濃度に少なくとも等しいかまたはそれを超える、請求項４〜６のいずれか1項に記載の組成物。
  8. 該少なくとも１種のＡＴＰ抽出剤が、グラム陰性細菌の完全性を破壊することができる化合物または化合物の組み合わせを含む、請求項１〜７のいずれか1項に記載の組成物。
  9. 該少なくとも１種のＡＴＰ抽出剤が、四級アンモニウム塩を含む請求項８に記載の組成物。
  10. 該少なくとも１種のＡＴＰ抽出剤がセチルトリメチル臭化アンモニウムを含んでなる、請求項８に記載の組成物。
  11. 該少なくとも１種のＡＴＰ抽出剤がクロロヘキシジンおよび非イオン性洗剤を含んでなる、請求項８に記載の組成物。
  12. 該少なくとも１種のＡＴＰ抽出剤が、セチルトリメチル臭化アンモニウム、クロロヘキシジン、および非イオン性洗剤を含んでなる、請求項８に記載の組成物。
  13. 二価カチオンがＭｇ2+、Ｃａ2+またはＭｎ2+である、請求項１〜１２のいずれか1項に記載の組成物。
  14. 二価カチオンキレート剤がＥＤＴＡまたはＣＤＴＡである、請求項１〜１２のいずれか1項に記載の組成物。
  15. 二価カチオンがＭｇ2+であり、二価カチオンキレート剤がＥＤＴＡである、請求項１〜１２のいずれか1項に記載の組成物。
  16. （ａ）サンプルと、請求項１〜１５のいずれか1項に記載の組成物とを接触させるステップと、
    （ｂ）ルミネセンスを検出するステップ
    を含んでなる、微生物を含有するまたは含有することが疑われるサンプル中のＡＴＰを検出する方法。
JP2007520407A 2004-07-02 2005-07-01 微生物ａｔｐの抽出及び検出システム Active JP5255273B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US58497104P 2004-07-02 2004-07-02
US60/584,971 2004-07-02
PCT/US2005/023545 WO2006085972A2 (en) 2004-07-02 2005-07-01 Compositions and processes for the extraction and detection of microbial atp

Publications (3)

Publication Number Publication Date
JP2008504841A JP2008504841A (ja) 2008-02-21
JP2008504841A5 true JP2008504841A5 (ja) 2008-08-21
JP5255273B2 JP5255273B2 (ja) 2013-08-07

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JP2007520407A Active JP5255273B2 (ja) 2004-07-02 2005-07-01 微生物ａｔｐの抽出及び検出システム

Country Status (10)

Country Link
US (2) US7422868B2 (ja)
EP (1) EP1763584B1 (ja)
JP (1) JP5255273B2 (ja)
CN (1) CN101040054A (ja)
AT (1) ATE429507T1 (ja)
CA (1) CA2572608A1 (ja)
DE (1) DE602005014110D1 (ja)
DK (1) DK1763584T3 (ja)
ES (1) ES2324952T3 (ja)
WO (1) WO2006085972A2 (ja)

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