JP2008206521A5 - - Google Patents
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- Publication number
- JP2008206521A5 JP2008206521A5 JP2008077932A JP2008077932A JP2008206521A5 JP 2008206521 A5 JP2008206521 A5 JP 2008206521A5 JP 2008077932 A JP2008077932 A JP 2008077932A JP 2008077932 A JP2008077932 A JP 2008077932A JP 2008206521 A5 JP2008206521 A5 JP 2008206521A5
- Authority
- JP
- Japan
- Prior art keywords
- endonuclease
- fen
- nucleic acid
- kit
- cleavage structure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004150 Flap endonucleases Human genes 0.000 claims 16
- 108090000652 Flap endonucleases Proteins 0.000 claims 16
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- 238000003776 cleavage reaction Methods 0.000 claims 7
- 238000000034 method Methods 0.000 claims 7
- 230000007017 scission Effects 0.000 claims 7
- 241000894007 species Species 0.000 claims 4
- 108091034117 Oligonucleotide Proteins 0.000 claims 3
- 241000203407 Methanocaldococcus jannaschii Species 0.000 claims 2
- 241001302042 Methanothermobacter thermautotrophicus Species 0.000 claims 2
- 241000205160 Pyrococcus Species 0.000 claims 2
- 241000205156 Pyrococcus furiosus Species 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 241000203069 Archaea Species 0.000 claims 1
- 102000004533 Endonucleases Human genes 0.000 claims 1
- 108010042407 Endonucleases Proteins 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 108020004511 Recombinant DNA Proteins 0.000 claims 1
- 238000010306 acid treatment Methods 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/757,653 US5843669A (en) | 1996-01-24 | 1996-11-29 | Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases |
| US08/758,314 US6090606A (en) | 1996-01-24 | 1996-12-02 | Cleavage agents |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007184453A Division JP4191228B2 (ja) | 1996-11-29 | 2007-07-13 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009185376A Division JP2009297032A (ja) | 1996-11-29 | 2009-08-10 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008206521A JP2008206521A (ja) | 2008-09-11 |
| JP2008206521A5 true JP2008206521A5 (https=) | 2009-02-05 |
Family
ID=27116427
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP52404398A Expired - Lifetime JP4362150B2 (ja) | 1996-11-29 | 1997-11-26 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
| JP2007184453A Expired - Lifetime JP4191228B2 (ja) | 1996-11-29 | 2007-07-13 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
| JP2008077932A Pending JP2008206521A (ja) | 1996-11-29 | 2008-03-25 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
| JP2009185376A Pending JP2009297032A (ja) | 1996-11-29 | 2009-08-10 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP52404398A Expired - Lifetime JP4362150B2 (ja) | 1996-11-29 | 1997-11-26 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
| JP2007184453A Expired - Lifetime JP4191228B2 (ja) | 1996-11-29 | 2007-07-13 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009185376A Pending JP2009297032A (ja) | 1996-11-29 | 2009-08-10 | Fen−1エンドヌクレアーゼ、混合物、および開裂方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US6562611B1 (https=) |
| EP (3) | EP2055781B1 (https=) |
| JP (4) | JP4362150B2 (https=) |
| AT (2) | ATE530652T1 (https=) |
| AU (1) | AU737449B2 (https=) |
| CA (1) | CA2273204C (https=) |
| DE (1) | DE69739108D1 (https=) |
| DK (1) | DK0966542T3 (https=) |
| ES (2) | ES2375764T3 (https=) |
| WO (1) | WO1998023774A1 (https=) |
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1997
- 1997-11-26 WO PCT/US1997/021783 patent/WO1998023774A1/en not_active Ceased
- 1997-11-26 DK DK97952237T patent/DK0966542T3/da active
- 1997-11-26 JP JP52404398A patent/JP4362150B2/ja not_active Expired - Lifetime
- 1997-11-26 ES ES08019704T patent/ES2375764T3/es not_active Expired - Lifetime
- 1997-11-26 DE DE69739108T patent/DE69739108D1/de not_active Expired - Lifetime
- 1997-11-26 EP EP08019704A patent/EP2055781B1/en not_active Expired - Lifetime
- 1997-11-26 AU AU55898/98A patent/AU737449B2/en not_active Expired
- 1997-11-26 US US09/308,825 patent/US6562611B1/en not_active Expired - Lifetime
- 1997-11-26 CA CA002273204A patent/CA2273204C/en not_active Expired - Lifetime
- 1997-11-26 EP EP10012561A patent/EP2374890B1/en not_active Expired - Lifetime
- 1997-11-26 ES ES97952237T patent/ES2317658T3/es not_active Expired - Lifetime
- 1997-11-26 AT AT08019704T patent/ATE530652T1/de not_active IP Right Cessation
- 1997-11-26 AT AT97952237T patent/ATE414167T1/de not_active IP Right Cessation
- 1997-11-26 EP EP97952237A patent/EP0966542B1/en not_active Expired - Lifetime
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2000
- 2000-10-06 US US09/684,938 patent/US6555357B1/en not_active Expired - Lifetime
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2007
- 2007-07-13 JP JP2007184453A patent/JP4191228B2/ja not_active Expired - Lifetime
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2008
- 2008-03-25 JP JP2008077932A patent/JP2008206521A/ja active Pending
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2009
- 2009-08-10 JP JP2009185376A patent/JP2009297032A/ja active Pending
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