JP2007525661A - 大分子その他の分析物の検出用生物センサー - Google Patents

大分子その他の分析物の検出用生物センサー Download PDF

Info

Publication number
JP2007525661A
JP2007525661A JP2006543991A JP2006543991A JP2007525661A JP 2007525661 A JP2007525661 A JP 2007525661A JP 2006543991 A JP2006543991 A JP 2006543991A JP 2006543991 A JP2006543991 A JP 2006543991A JP 2007525661 A JP2007525661 A JP 2007525661A
Authority
JP
Japan
Prior art keywords
aptamer
epitope
polypeptide
construct
label
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2006543991A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007525661A5 (https=
Inventor
ヘイダック、トマーズ
ヘイダック、エワ
クノール、エリック
Original Assignee
セントルイス ユニバーシティー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by セントルイス ユニバーシティー filed Critical セントルイス ユニバーシティー
Publication of JP2007525661A publication Critical patent/JP2007525661A/ja
Publication of JP2007525661A5 publication Critical patent/JP2007525661A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2006543991A 2003-12-12 2004-12-10 大分子その他の分析物の検出用生物センサー Pending JP2007525661A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52907603P 2003-12-12 2003-12-12
PCT/US2004/041315 WO2005059509A2 (en) 2003-12-12 2004-12-10 Biosensors for detecting macromolecules and other analytes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2011284014A Division JP2012115269A (ja) 2003-12-12 2011-12-26 大分子その他の分析物の検出用生物センサー

Publications (2)

Publication Number Publication Date
JP2007525661A true JP2007525661A (ja) 2007-09-06
JP2007525661A5 JP2007525661A5 (https=) 2008-01-31

Family

ID=34699935

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2006543991A Pending JP2007525661A (ja) 2003-12-12 2004-12-10 大分子その他の分析物の検出用生物センサー
JP2011284014A Pending JP2012115269A (ja) 2003-12-12 2011-12-26 大分子その他の分析物の検出用生物センサー

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2011284014A Pending JP2012115269A (ja) 2003-12-12 2011-12-26 大分子その他の分析物の検出用生物センサー

Country Status (6)

Country Link
US (2) US7939313B2 (https=)
EP (1) EP1692486B1 (https=)
JP (2) JP2007525661A (https=)
CN (1) CN101208437B (https=)
CA (1) CA2545006C (https=)
WO (1) WO2005059509A2 (https=)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009149549A (ja) * 2007-12-19 2009-07-09 Japan Science & Technology Agency ペプチドアプタマーライブラリーの作製方法および用途
JP2011505549A (ja) * 2007-11-21 2011-02-24 エラスムス ユニバーシティ メディカル センター ロッテルダム 改良型ｆｒｅｔプローブおよびその使用
JP2014504299A (ja) * 2010-12-23 2014-02-20 エフ．ホフマン−ラ ロシュ アーゲー ポリペプチド−ポリヌクレオチド複合体、およびエフェクター成分の標的化された送達におけるその使用
JP2014533364A (ja) * 2011-11-11 2014-12-11 イーライ・エヌ・グレザー コバインダー補助アッセイ法
WO2016047068A1 (ja) * 2014-09-22 2016-03-31 国立研究開発法人科学技術振興機構 ターゲット分子の検出方法及びこれに用いられるキット
JP2017533417A (ja) * 2014-09-30 2017-11-09 コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． 第１及び第２のエピトープの空間的近接を検出する方法
WO2019103131A1 (ja) * 2017-11-24 2019-05-31 国立大学法人 東京大学 アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法
JP2020162612A (ja) * 2013-10-28 2020-10-08 ドッツ テクノロジー コーポレイションＤＯＴＳ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｒｐ． アレルゲン検出のためのシグナル発生ポリヌクレオチド
JP2022551736A (ja) * 2019-10-15 2022-12-13 ユニバーシティ オブ フロリダ リサーチ ファウンデーション，インコーポレイティド 血中のアンチトロンビンｉｉｉを定量するためのアプタマーベースのシステム
US11525825B2 (en) 2014-05-15 2022-12-13 Meso Scale Technologies, Llc. Assay kits
US11697840B2 (en) 2013-03-13 2023-07-11 Meso Scale Technologies, Llc. Method of detecting analyte in a sample with binding reagent, first detection reagent, and second detection reagent
JP2023168019A (ja) * 2022-05-13 2023-11-24 国立大学法人北海道大学 フォン・ヴィレブランド因子の情報を取得する方法、測定試料の調製方法及び試薬キット
US12163954B2 (en) 2013-03-13 2024-12-10 Meso Scale Technologies, Llc. Assay methods
JP2025072414A (ja) * 2019-06-25 2025-05-09 アクトーメ ゲーエムベーハー ２成分系の分析物を測定するための方法およびキットならびにそれらの使用
US12553887B2 (en) 2019-03-01 2026-02-17 Meso Scale Technologies, Llc. Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101208437B (zh) 2003-12-12 2012-07-04 圣路易斯大学 检测大分子和其它分析物的生物传感器
KR20080028886A (ko) * 2005-05-26 2008-04-02 앙상블 디스커버리 코포레이션 핵산-템플레이팅된 화학 반응에 의한 생검출
US7795009B2 (en) 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
EP1907410B1 (en) 2005-06-10 2012-07-04 Saint Louis University Methods for the selection of aptamers
US7811809B2 (en) 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
BRPI0614961A2 (pt) * 2005-08-26 2016-09-13 Archemix Corp aptâmero, método, e, composição
EP2046998B1 (en) * 2006-08-09 2013-10-02 Saint Louis University Molecular biosensors for detecting macromolecules and other analytes
US8664364B2 (en) * 2007-01-24 2014-03-04 Carnegie Mellon University Optical biosensors
DE102007063902B3 (de) * 2007-08-31 2017-11-23 Rheinische Friedrich-Wilhelms-Universität Bonn Aptamere, die an ein an der Hämostase beteiligtes Zielmolekül binden
DE102007041476B4 (de) 2007-08-31 2017-03-30 Rheinische Friedrich-Wilhelms-Universität Bonn Aptamere, die an ein an der Hämostase beteiligtes Zielmolekül binden
US8426153B2 (en) 2007-12-03 2013-04-23 Carnegie Mellon University Linked peptides fluorogenic biosensors
US20140128280A1 (en) * 2008-04-23 2014-05-08 Arizona Board of Regents, a body corporate of the State of Arizona, acting for and on behaif of Ariz Synthetic Antibodies
BRPI0920985A2 (pt) * 2008-11-21 2015-08-18 Univ Columbia Enzima de dna dividida para tipificação visual de polimorfismo de nucleotídeo único
EP2350657A4 (en) * 2008-11-21 2013-06-12 Univ Saint Louis BIOSENSOR FOR DETECTING MULTIPLE EPITOPES ON ONE TARGET
US9040287B2 (en) 2010-02-12 2015-05-26 Mediomics, Llc Molecular biosensors capable of signal amplification
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
HRP20151294T1 (hr) 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
TWI497076B (zh) 2010-06-25 2015-08-21 Univ Nat Cheng Kung 適合體及ｃ－反應蛋白檢測方法
EP2479914B1 (en) * 2011-01-21 2015-03-04 Alcatel Lucent Method and transmitter element for transmitting channel information for link adaption, method and receiver element for receiving the channel information
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
FR2977674B1 (fr) * 2011-07-06 2015-08-14 Cisbio Bioassays Methode amelioree de detection et/ou de quantification d'un analyte present a la surface d'une cellule
CN103160512B (zh) * 2011-12-15 2014-09-24 国家纳米科学中心 一种核酸及药物载体和药物组合物及其制备方法
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2014102803A1 (en) * 2012-12-31 2014-07-03 Yeda Research And Development Co. Ltd. Molecular sensor and methods of use thereof
CN105378477B9 (zh) * 2013-03-11 2021-08-06 梅索磅秤技术有限公司 用于进行多路测定法的改进方法
EP3013983B1 (en) 2013-06-25 2023-02-15 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
CN103630517B (zh) * 2013-11-15 2015-12-09 南京邮电大学 一种基于拆分适配体和水溶性共轭聚合物的凝血酶检测方法
CN104020293A (zh) * 2014-03-25 2014-09-03 李根喜 基于多肽的黑色素瘤生物标记物传感器
US11639929B2 (en) 2014-04-29 2023-05-02 Yeda Research And Development Co. Ltd. Universal histidine-tag binding compounds and methods of use thereof as fluorescent probes and sensors
US12332246B2 (en) 2014-04-29 2025-06-17 Yeda Research And Development Co. Ltd. Quinoline based cyanine dye turn-on fluorescent probes and methods of use thereof
EP3139942B1 (en) 2014-05-05 2019-12-18 Bioventures, Llc COMPOSITIONS AND METHODS FOR INHIBITING ANTIAPOPTOTIC Bcl-2 PROTEINS AS ANTI-AGING AGENTS
US10071087B2 (en) 2014-07-22 2018-09-11 Bioventures, Llc Compositions and methods for selectively depleting senescent cells
EP2980218A1 (en) * 2014-07-30 2016-02-03 Rheinische Friedrich-Wilhelms-Universität Bonn Aptamer thrombin complex for use as an antidote to direct acting thrombin inhibitors
WO2016040638A2 (en) 2014-09-10 2016-03-17 Washington University Compositions and methods for treatment of pre-cancerous skin lesions
WO2016040830A1 (en) 2014-09-12 2016-03-17 Mediomics, Llc Molecular biosensors with a modular design
US10545158B2 (en) 2014-12-29 2020-01-28 North Carolina State University Multiplexed diagnostic to recognize concentrations of related proteins and peptides
CN104569430B (zh) * 2015-01-04 2017-01-04 深圳市艾瑞生物科技有限公司 一种快速定量检测心脏型脂肪酸结合蛋白的均相荧光免疫试剂组及其制备方法
US10774374B2 (en) 2015-04-10 2020-09-15 Spatial Transcriptomics AB and Illumina, Inc. Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US20180251765A1 (en) * 2015-09-08 2018-09-06 BellBrook Labs High-throughput split aptamer screening assay
WO2017120589A1 (en) 2016-01-08 2017-07-13 Washington University Compositions comprising chemerin and methods of use thereof
CN105543231B (zh) * 2016-01-11 2019-07-05 河南省农业科学院农业质量标准与检测技术研究所 伏马毒素b1核酸适配体链置换探针的筛选及应用
ES2924495T3 (es) 2016-01-29 2022-10-07 Univ Washington GDF15 en glaucoma y métodos de uso de este
JP6936498B2 (ja) 2016-04-21 2021-09-15 バイオベンチャーズ・リミテッド・ライアビリティ・カンパニーＢｉｏＶｅｎｔｕｒｅｓ， ＬＬＣ 抗アポトーシス性Ｂｃｌ−２ファミリータンパク質の分解を誘導する化合物及びその使用
WO2017189846A1 (en) 2016-04-27 2017-11-02 Thaddeus Stappenbeck Methods for prognosing crohn's disease comprising human defensin 5 (hd5)
JP2019532301A (ja) * 2016-07-15 2019-11-07 イ，ジフン 血液凝固因子検出のための化学発光バイオセンサー
US11058653B2 (en) 2016-10-26 2021-07-13 Washington University Compositions comprising desaminotyrosine and uses thereof to enhance type I interferon stimulation
US11175296B2 (en) 2016-10-26 2021-11-16 Washington University Methods of diagnosing and treating cancer comprising ME1
EP3562953A1 (en) 2016-12-30 2019-11-06 Quidel Corporation Phage-mediated immunoassay and methods for determining susceptibility of bacteria to antibiotic or probiotic agents
US20200000934A1 (en) 2017-02-28 2020-01-02 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Conjugates comprising ocular angiogenesis growth factor aptamers and uses thereof in the detection and treatment of ophthalmological angiogenesis indications
EP3743069B1 (en) 2018-01-22 2025-08-06 BioVentures, LLC Bcl-2 proteins degraders for cancer treatment
US11085935B2 (en) 2018-05-03 2021-08-10 Washington University Methods of treating based on site-specific tau phosphorylation
US12084423B2 (en) 2018-05-18 2024-09-10 Bioventures, Llc Piperlongumine analogues and uses thereof
CN108956991B (zh) * 2018-07-25 2021-04-27 南通大学 一种荧光共振能量转移生物传感器及其应用
CN109444103B (zh) * 2018-12-18 2021-09-28 咸阳师范学院 一种pei功能化绿色荧光碳点制备方法及基于该碳点的凝血酶检测方法
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
KR20220041072A (ko) 2019-06-05 2022-03-31 엘아이브이 프로세스, 인크. 클로스트리듐 디피실에 대한 앱타머
US20220236257A1 (en) * 2019-06-05 2022-07-28 Yeda Research And Development Co. Ltd. Artificial receptors, recombinant cells comprising thereof, methods for their preparation, and method of using thereof
WO2020252086A1 (en) 2019-06-10 2020-12-17 Washington University Microbiota-directed foods to repair a subject's gut microbiota
EP4028775A4 (en) 2019-09-10 2023-04-12 Washington University BLOOD-BASED TEST FOR DIAGNOSIS AND TREATMENT BASED ON SITE-SPECIFIC TAU PHOSPHORYLATION
CN110672567B (zh) * 2019-09-26 2022-01-11 南通大学 低分子量肝素金纳米材料及其在乙酰肝素酶检测中的应用
EP4058810A4 (en) * 2019-11-15 2023-12-20 President And Fellows Of Harvard College DEVICE AND METHOD FOR ANALYTE DETECTION
WO2021150578A1 (en) * 2020-01-20 2021-07-29 Arizona Board Of Regents On Behalf Of The University Of Arizona Fluorescently-labeled f-actin protein biosensors and methods of high-throughput drug discovery
CN113624724A (zh) * 2020-05-07 2021-11-09 廖世奇 一种适配体分子信标对靶分子的多元检测分析方法
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
EP4158054B1 (en) 2020-06-02 2025-04-16 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US11898146B2 (en) 2020-12-03 2024-02-13 Liv Process, Inc. Aptamers against Clostridium difficile, compositions comprising aptamers against Clostridium difficile and methods of using the same
CN113702308B (zh) * 2021-08-25 2024-01-30 青岛大学 用于大肠杆菌检测的适配体纳米比色生物传感器及应用
NL2032916B1 (en) * 2022-08-31 2024-03-15 Univ Delft Tech Single-molecule aptamer FRET for protein identification and structural analysis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002056024A2 (en) * 2001-01-12 2002-07-18 Affibody Ab Detection methods
JP2003508729A (ja) * 1999-05-14 2003-03-04 ブランディーズ・ユニバーシティ 核酸をベースとする検出
WO2003078449A2 (en) * 2001-08-13 2003-09-25 Saint Louis University A rapid and sensitive proximity-based assay for the detection and quantification of dna binding proteins

Family Cites Families (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6001577A (en) * 1998-06-08 1999-12-14 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US6127119A (en) * 1990-06-11 2000-10-03 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US6147204A (en) * 1990-06-11 2000-11-14 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand complexes
US5683867A (en) * 1990-06-11 1997-11-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: blended SELEX
US5496938A (en) * 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US5874218A (en) * 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound in a substance using a nucleic acid ligand
US6261774B1 (en) * 1990-06-11 2001-07-17 Gilead Sciences, Inc. Truncation selex method
US6030776A (en) * 1990-06-11 2000-02-29 Nexstar Pharmaceuticals, Inc. Parallel SELEX
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5270163A (en) * 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5789157A (en) * 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5567588A (en) * 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5853984A (en) * 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
US5705337A (en) * 1990-06-11 1998-01-06 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US5650275A (en) * 1990-06-11 1997-07-22 Nexstar Pharmacueticals Inc Target detection method using spectroscopically detectable nucleic acid ligands
US6083696A (en) * 1990-06-11 2000-07-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands exponential enrichment: blended selex
US5637459A (en) * 1990-06-11 1997-06-10 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chimeric selex
US5864026A (en) * 1990-06-11 1999-01-26 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5641629A (en) * 1990-06-11 1997-06-24 Nexstar Pharmacueticals Inc Spectroscopically detectable nucleic acid ligands
EP0786469B1 (en) * 1990-06-11 2006-03-01 Gilead Sciences, Inc. Methods of use of nucleic acid ligands
US5723289A (en) * 1990-06-11 1998-03-03 Nexstar Pharmaceuticals, Inc. Parallel selex
US6011020A (en) * 1990-06-11 2000-01-04 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand complexes
US5763566A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US5476766A (en) * 1990-06-11 1995-12-19 Nexstar Pharmaceuticals, Inc. Ligands of thrombin
US5543293A (en) * 1990-06-11 1996-08-06 Nexstar Pharmaceuticals, Inc. DNA ligands of thrombin
US5712375A (en) * 1990-06-11 1998-01-27 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5962219A (en) * 1990-06-11 1999-10-05 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-selex
US5750342A (en) * 1990-06-11 1998-05-12 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US5688935A (en) * 1990-06-11 1997-11-18 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US5861254A (en) * 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US6465188B1 (en) * 1990-06-11 2002-10-15 Gilead Sciences, Inc. Nucleic acid ligand complexes
US5840867A (en) * 1991-02-21 1998-11-24 Gilead Sciences, Inc. Aptamer analogs specific for biomolecules
US5582981A (en) * 1991-08-14 1996-12-10 Gilead Sciences, Inc. Method for identifying an oligonucleotide aptamer specific for a target
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US5998142A (en) * 1993-09-08 1999-12-07 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US6048698A (en) * 1994-09-20 2000-04-11 Nexstar Pharmaceuticals, Inc. Parallel SELEX™
US6013443A (en) * 1995-05-03 2000-01-11 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
DE69633227T2 (de) * 1995-05-03 2005-09-15 Gilead Sciences, Inc., Foster City Systematische evoultion von liganden durch exponentielle anreicherung: gewebe-selex
US5582961A (en) * 1995-06-06 1996-12-10 Eastman Kodak Company Photographic elements which achieve colorimetrically accurate recording
SE504798C2 (sv) 1995-06-16 1997-04-28 Ulf Landegren Immunanalys och testkit med två reagens som kan tvärbindas om de adsorberats till analyten
US6001570A (en) * 1997-02-18 1999-12-14 Invitro Diagnostics, Inc. Compositions, methods, kits and apparatus for determining the presence or absence of target molecules
US5989823A (en) * 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
EP1049803A4 (en) * 1997-12-15 2002-08-21 Nexstar Pharmaceuticals Inc HOMOGENEOUS DETECTION OF TARGET MOLECULES BY LIGAND NUCLEIC ACID AND LIGAND BEACON INTERACTION
US6225058B1 (en) * 1998-01-13 2001-05-01 Invitro Diagnostics, Inc. Compositions, methods, kits and apparatus for determining the presence or absence of target molecules
WO1999037806A2 (en) * 1998-01-27 1999-07-29 Cytocell Limited Modified nucleic acid probes and uses thereof
US6287772B1 (en) * 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6399302B1 (en) * 1998-08-21 2002-06-04 University Of Virginia Patent Foundation Signal generating oligonucleotide-based biosensor
US6867289B1 (en) * 1998-10-26 2005-03-15 Board Of Regents, The University Of Texas Systems Thio-modified aptamer synthetic methods and compositions
US6329145B1 (en) * 1999-02-09 2001-12-11 Gilead Science, Inc. Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
US6680377B1 (en) * 1999-05-14 2004-01-20 Brandeis University Nucleic acid-based detection
US6506887B1 (en) * 1999-07-29 2003-01-14 Somalogic, Incorporated Conditional-selex
US20030232388A1 (en) * 1999-09-27 2003-12-18 Kreimer David I. Beads having identifiable Raman markers
US7306904B2 (en) * 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
US20020037506A1 (en) * 2000-04-18 2002-03-28 Yun Lin Aptamer based two-site binding assay
US20020022224A1 (en) * 2000-04-28 2002-02-21 Hornby David P. Method for isolating single-stranded DNA
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US6451588B1 (en) * 2000-06-30 2002-09-17 Pe Corporation (Ny) Multipartite high-affinity nucleic acid probes
US20030207271A1 (en) * 2000-06-30 2003-11-06 Holwitt Eric A. Methods and compositions for biological sensors
US6380377B1 (en) 2000-07-14 2002-04-30 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US7125660B2 (en) * 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
EP1354062A2 (en) * 2000-09-13 2003-10-22 Archemix Corporation Target activated nucleic acid biosensor and methods of using same
US6376190B1 (en) * 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
DE10048944A1 (de) * 2000-10-03 2002-04-18 Andreas Kage Verfahren zur Selektion hochaffin an ein Target bindender Nukleinsäuren durch zweidimensionale Auftrennung
CA2423729A1 (en) * 2000-10-06 2002-04-11 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
AU2775402A (en) * 2001-06-29 2003-01-02 Medimolecular Pty Ltd Nucleic acid ligands to complex targets
US7172865B2 (en) 2001-08-13 2007-02-06 Saint Louis University Rapid and sensitive assay for the detection and quantification of coregulators of nucleic acid binding factors
US6593091B2 (en) * 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US20030232383A1 (en) * 2001-11-02 2003-12-18 Sylvia Daunert Novel reagentless sensing system for measuring carbohydrates based on the galactose/glucose binding protein
DE10161424A1 (de) * 2001-12-13 2003-07-03 Winkler & Duennebier Ag Verfahren und Vorrichtung zum Erkennen von Produktfehlern bei der Herstellung von Briefhüllen, Versandtaschen und dergleichen
US6688081B2 (en) * 2001-12-18 2004-02-10 Schmalbach-Lubeca Ag Method for reducing headspace gas
CN1314810C (zh) 2002-01-31 2007-05-09 圣路易大学 一种用来检测和定量核酸结合因子辅调节物的快速灵敏测定法
US20030224435A1 (en) * 2002-05-16 2003-12-04 Scott Seiwert Detection of abused substances and their metabolites using nucleic acid sensor molecules
US7435542B2 (en) * 2002-06-24 2008-10-14 Cornell Research Foundation, Inc. Exhaustive selection of RNA aptamers against complex targets
JP4632788B2 (ja) * 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
AU2003294437A1 (en) * 2002-11-21 2004-06-18 Archemix Corporation Multivalent aptamer therapeutics with improved pharmacodynamic properties and methods of making and using the same
AU2004232976B2 (en) 2003-04-18 2011-02-10 Becton, Dickinson And Company Immuno-amplification
US20050106594A1 (en) * 2003-08-22 2005-05-19 Andrew Ellington In vitro selection of aptamer beacons
US20050095627A1 (en) * 2003-09-03 2005-05-05 The Salk Institute For Biological Studies Multiple antigen detection assays and reagents
US7419835B2 (en) * 2003-11-21 2008-09-02 Palo Alto Research Center Incorporated Screening for ligand binding at specific target sites
CN101208437B (zh) 2003-12-12 2012-07-04 圣路易斯大学 检测大分子和其它分析物的生物传感器
WO2005089524A2 (en) 2004-03-19 2005-09-29 U.S. Genomics, Inc. Compositions and methods for detection of single molecules
US7795009B2 (en) * 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
EP1907410B1 (en) 2005-06-10 2012-07-04 Saint Louis University Methods for the selection of aptamers
US7811809B2 (en) * 2005-06-15 2010-10-12 Saint Louis University Molecular biosensors for use in competition assays
JP5142290B2 (ja) 2006-09-27 2013-02-13 国立大学法人東京農工大学 試料中の被検物質の測定方法並びにアプタマー分子及びその作出方法
EP2350657A4 (en) 2008-11-21 2013-06-12 Univ Saint Louis BIOSENSOR FOR DETECTING MULTIPLE EPITOPES ON ONE TARGET

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003508729A (ja) * 1999-05-14 2003-03-04 ブランディーズ・ユニバーシティ 核酸をベースとする検出
WO2002056024A2 (en) * 2001-01-12 2002-07-18 Affibody Ab Detection methods
WO2003078449A2 (en) * 2001-08-13 2003-09-25 Saint Louis University A rapid and sensitive proximity-based assay for the detection and quantification of dna binding proteins

Cited By (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011505549A (ja) * 2007-11-21 2011-02-24 エラスムス ユニバーシティ メディカル センター ロッテルダム 改良型ｆｒｅｔプローブおよびその使用
JP2009149549A (ja) * 2007-12-19 2009-07-09 Japan Science & Technology Agency ペプチドアプタマーライブラリーの作製方法および用途
JP2014504299A (ja) * 2010-12-23 2014-02-20 エフ．ホフマン−ラ ロシュ アーゲー ポリペプチド−ポリヌクレオチド複合体、およびエフェクター成分の標的化された送達におけるその使用
US11242570B2 (en) 2011-11-11 2022-02-08 Meso Scale Technologies, Llc. Co-binder assisted assay methods
JP2020126068A (ja) * 2011-11-11 2020-08-20 イーライ・エヌ・グレザー コバインダー補助アッセイ法
US12359264B2 (en) 2011-11-11 2025-07-15 Meso Scale Technologies, Llc. Co-binder assisted assay methods
US9777338B2 (en) 2011-11-11 2017-10-03 Meso Scale Technologies, Llc. Co-binder assisted assay methods
JP2018054629A (ja) * 2011-11-11 2018-04-05 イーライ・エヌ・グレザー コバインダー補助アッセイ法
JP2014533364A (ja) * 2011-11-11 2014-12-11 イーライ・エヌ・グレザー コバインダー補助アッセイ法
US12163954B2 (en) 2013-03-13 2024-12-10 Meso Scale Technologies, Llc. Assay methods
US11697840B2 (en) 2013-03-13 2023-07-11 Meso Scale Technologies, Llc. Method of detecting analyte in a sample with binding reagent, first detection reagent, and second detection reagent
JP6992126B2 (ja) 2013-10-28 2022-02-03 ドッツ テクノロジー コーポレイション アレルゲン検出のためのシグナル発生ポリヌクレオチド
JP2020162612A (ja) * 2013-10-28 2020-10-08 ドッツ テクノロジー コーポレイションＤＯＴＳ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｒｐ． アレルゲン検出のためのシグナル発生ポリヌクレオチド
US11268136B2 (en) 2013-10-28 2022-03-08 Dots Technology Corp. Allergen detection
US12411131B2 (en) 2014-05-15 2025-09-09 Meso Scale Technologies, Llc. Assay methods
US11525825B2 (en) 2014-05-15 2022-12-13 Meso Scale Technologies, Llc. Assay kits
JP2025020158A (ja) * 2014-05-15 2025-02-12 メソ スケール テクノロジーズ エルエルシー 改善されたアッセイ方法
KR102461615B1 (ko) * 2014-09-22 2022-11-01 고쿠리츠켄큐카이하츠호진 카가쿠기쥬츠신코키코 타겟 분자의 검출 방법 및 이것에 사용되는 키트
WO2016047068A1 (ja) * 2014-09-22 2016-03-31 国立研究開発法人科学技術振興機構 ターゲット分子の検出方法及びこれに用いられるキット
JPWO2016047068A1 (ja) * 2014-09-22 2017-07-06 国立研究開発法人科学技術振興機構 ターゲット分子の検出方法及びこれに用いられるキット
KR20170057241A (ko) * 2014-09-22 2017-05-24 고쿠리츠켄큐카이하츠호진 카가쿠기쥬츠신코키코 타겟 분자의 검출 방법 및 이것에 사용되는 키트
JP2017533417A (ja) * 2014-09-30 2017-11-09 コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． 第１及び第２のエピトープの空間的近接を検出する方法
WO2019103131A1 (ja) * 2017-11-24 2019-05-31 国立大学法人 東京大学 アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法
US12553887B2 (en) 2019-03-01 2026-02-17 Meso Scale Technologies, Llc. Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same
JP2025072414A (ja) * 2019-06-25 2025-05-09 アクトーメ ゲーエムベーハー ２成分系の分析物を測定するための方法およびキットならびにそれらの使用
JP7621666B2 (ja) 2019-10-15 2025-01-27 ユニバーシティ オブ フロリダ リサーチ ファウンデーション，インコーポレイティド 血中のアンチトロンビンｉｉｉを定量するためのアプタマーベースのシステム
JP2022551736A (ja) * 2019-10-15 2022-12-13 ユニバーシティ オブ フロリダ リサーチ ファウンデーション，インコーポレイティド 血中のアンチトロンビンｉｉｉを定量するためのアプタマーベースのシステム
JP2023168019A (ja) * 2022-05-13 2023-11-24 国立大学法人北海道大学 フォン・ヴィレブランド因子の情報を取得する方法、測定試料の調製方法及び試薬キット
JP7832621B2 (ja) 2022-05-13 2026-03-18 国立大学法人北海道大学 フォン・ヴィレブランド因子の情報を取得する方法、測定試料の調製方法及び試薬キット

Also Published As

Publication number Publication date
EP1692486A2 (en) 2006-08-23
US20110091893A1 (en) 2011-04-21
CN101208437B (zh) 2012-07-04
US7939313B2 (en) 2011-05-10
CN101208437A (zh) 2008-06-25
CA2545006A1 (en) 2005-06-30
EP1692486A4 (en) 2008-05-21
WO2005059509A3 (en) 2007-11-15
HK1120568A1 (en) 2009-04-03
US20060110739A1 (en) 2006-05-25
US8592202B2 (en) 2013-11-26
CA2545006C (en) 2013-09-17
EP1692486B1 (en) 2015-10-28
WO2005059509A2 (en) 2005-06-30
JP2012115269A (ja) 2012-06-21

Similar Documents

Publication Publication Date Title
EP1692486B1 (en) Biosensors for detecting macromolecules and other analytes
US7795009B2 (en) Three-component biosensors for detecting macromolecules and other analytes
US7811809B2 (en) Molecular biosensors for use in competition assays
US8956857B2 (en) Three-component biosensors for detecting macromolecules and other analytes
Tang et al. In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection
Ilgu et al. Aptamers in analytics
Ebrahimi et al. Forced intercalation (FIT)-aptamers
Tombelli et al. Analytical applications of aptamers
AU784335B2 (en) Signaling aptamers that transduce molecular recognition to differential signal
JP2007525661A5 (https=)
Nie et al. A self-assemble aptamer fragment/target complex based high-throughput colorimetric aptasensor using enzyme linked aptamer assay
CA2660129C (en) Molecular biosensors for detecting macromolecules and other analytes
US20220243196A1 (en) Screening method of aptamer and immunoassay using the aptamer
Tombelli et al. Aptamers biosensors for pharmaceutical compounds
CN112424371A (zh) 蛋白质-多核苷酸缀合物的检测测定
HK1120568B (en) Biosensors for detecting macromolecules and other analytes
AU2006226844A1 (en) Elucidation of high affinity, high specificity oligonucleotides and derivatized oligonucleotide sequences for target recognition

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20071127

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20071127

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100223

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20100524

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20100531

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100823

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20101124

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110223

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110302

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110524

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20110823

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111226