JP2006512921A - 多量体タンパク質の生産 - Google Patents

多量体タンパク質の生産 Download PDF

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JP2006512921A
JP2006512921A JP2004567446A JP2004567446A JP2006512921A JP 2006512921 A JP2006512921 A JP 2006512921A JP 2004567446 A JP2004567446 A JP 2004567446A JP 2004567446 A JP2004567446 A JP 2004567446A JP 2006512921 A JP2006512921 A JP 2006512921A
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sequence
gene
protein
polynucleotide
multimeric protein
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JP2006512921A5 (enExample
Inventor
ケイ． クーパー，リチャード
シー． フィオレッティ，ウィリアム
ジー． カッド，ゲイリー
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Louisiana State University
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Louisiana State University
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
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    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/02—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from eggs
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1036—Retroviridae, e.g. leukemia viruses
    • C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
    • C07K16/1063—Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
    • C—CHEMISTRY; METALLURGY
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    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/23—Immunoglobulins specific features characterized by taxonomic origin from birds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
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    • C12N2830/00—Vector systems having a special element relevant for transcription
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/90—Vector systems having a special element relevant for transcription from vertebrates avian
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Animal Behavior & Ethology (AREA)
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  • Biodiversity & Conservation Biology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2004567446A 2003-01-21 2003-12-24 多量体タンパク質の生産 Withdrawn JP2006512921A (ja)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US44139203P 2003-01-21 2003-01-21
US44144703P 2003-01-21 2003-01-21
US44137703P 2003-01-21 2003-01-21
US44138103P 2003-01-21 2003-01-21
US44150203P 2003-01-21 2003-01-21
US44140503P 2003-01-21 2003-01-21
US10/609,019 US7527966B2 (en) 2002-06-26 2003-06-26 Gene regulation in transgenic animals using a transposon-based vector
PCT/US2003/041261 WO2004067706A2 (en) 2003-01-21 2003-12-24 Production of multimeric proteins

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JP2006512921A true JP2006512921A (ja) 2006-04-20
JP2006512921A5 JP2006512921A5 (enExample) 2007-02-15

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JP2004567446A Withdrawn JP2006512921A (ja) 2003-01-21 2003-12-24 多量体タンパク質の生産
JP2004567449A Pending JP2006512922A (ja) 2003-01-21 2003-12-24 生殖器官へのトランスポゾンベクターの投与

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US (2) US7527966B2 (enExample)
EP (2) EP1592789B1 (enExample)
JP (2) JP2006512921A (enExample)
AT (1) ATE431849T1 (enExample)
AU (3) AU2003299959A1 (enExample)
DE (1) DE60327717D1 (enExample)
WO (3) WO2004067707A2 (enExample)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US20040235011A1 (en) * 2002-06-26 2004-11-25 Cooper Richard K. Production of multimeric proteins
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
US8071364B2 (en) * 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
US7718847B2 (en) * 2004-05-27 2010-05-18 Kyoto University Method of gene introduction in in-vivo spermatogenic cell
EP1885856B1 (en) 2005-05-17 2013-07-17 Temasek Life Sciences Laboratory Limited Transposition of maize ac/ds elements in vertebrates
KR100830826B1 (ko) 2007-01-24 2008-05-19 씨제이제일제당 (주) 코리네박테리아를 이용하여 글리세롤을 포함한탄소원으로부터 발효산물을 생산하는 방법
AU2007345347B2 (en) * 2007-01-26 2013-11-07 Synageva Biopharma Corp Transgene expression in avians
EP2527445A3 (en) * 2007-05-16 2013-02-20 MAT Malta Advanced Technologies Limited Treatment and prevention of influenza
US8932861B2 (en) 2008-04-10 2015-01-13 Cj Cheiljedang Corporation Transformation vector comprising transposon, microorganisms transformed with the vector, and method for producing L-lysine using the microorganism
KR101126041B1 (ko) * 2008-04-10 2012-03-19 씨제이제일제당 (주) 트랜스포존을 이용한 형질전환용 벡터, 상기 벡터로형질전환된 미생물 및 이를 이용한 ｌ-라이신 생산방법
WO2010008562A2 (en) * 2008-07-16 2010-01-21 Recombinetics Methods and materials for producing transgenic animals
WO2010036976A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of antibodies
EP2342224A2 (en) * 2008-09-25 2011-07-13 TransGenRx, Inc. Novel vectors for production of interferon
WO2010036978A2 (en) * 2008-09-25 2010-04-01 Transgenrx, Inc. Novel vectors for production of growth hormone
US9150881B2 (en) * 2009-04-09 2015-10-06 Proteovec Holding, L.L.C. Production of proteins using transposon-based vectors
US9314005B2 (en) 2009-07-01 2016-04-19 Transposagen Biopharmaceuticals, Inc. Genetically modified rat models for severe combined immunodeficiency (SCID)
EP2462230B1 (en) * 2009-08-03 2015-07-15 Recombinetics, Inc. Methods and compositions for targeted gene modification
WO2012051615A1 (en) 2010-10-15 2012-04-19 Transgenrx, Inc. Novel vectors for production of glycosylated interferon
AU2013204327B2 (en) * 2012-04-20 2016-09-01 Aviagen Cell transfection method
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
WO2014145736A2 (en) * 2013-03-15 2014-09-18 Transposagen Biopharmaceuticals, Inc. Reproducible method for testis-mediated genetic modification (tgm) and sperm-mediated genetic modification (sgm)
WO2014189957A2 (en) 2013-05-23 2014-11-27 The Board Of Trustees Of The Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
CN114534806B (zh) 2014-04-10 2024-03-29 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
PL3164130T3 (pl) 2014-07-01 2020-02-28 Millennium Pharmaceuticals, Inc. Związki heteroarylowe użyteczne jako inhibitory enzymu aktywującego sumo
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
US10934636B2 (en) 2015-08-12 2021-03-02 CeMM—FORSCHUNGSZENTRUM FÜR MOLEKULARE MEDIZIN GmbH Methods for studying nucleic acids
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US11733248B2 (en) 2017-09-25 2023-08-22 Fred Hutchinson Cancer Center High efficiency targeted in situ genome-wide profiling
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN119020348A (zh) 2018-02-05 2024-11-26 斯坦福大学托管董事会 用于单细胞和集合细胞的多重测量的系统和方法
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
KR20220080091A (ko) 2019-09-06 2022-06-14 체엠엠 - 포르슝스첸트룸 퓨어 몰레쿨라레 메디친 게엠베하 Rna 올리고뉴클레오티드를 시퀀싱하기 위한 방법
CN114317600A (zh) * 2020-10-12 2022-04-12 上海君赛生物科技有限公司 新型PiggyBac转座子系统及其用途
CN118510910A (zh) * 2022-01-10 2024-08-16 塞提斯生物技术有限公司 用于处理含核酸样品的方法
WO2025183084A1 (ja) * 2024-02-28 2025-09-04 国立研究開発法人産業技術総合研究所 蛋白質の分泌促進方法

Family Cites Families (166)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4870009A (en) 1982-11-22 1989-09-26 The Salk Institute For Biological Studies Method of obtaining gene product through the generation of transgenic animals
US4670388A (en) 1982-12-30 1987-06-02 Carnegie Institution Of Washington Method of incorporating DNA into genome of drosophila
US4914025A (en) 1985-12-05 1990-04-03 Colin Manoil Export of intra-cellular substances
EP0279582A3 (en) 1987-02-17 1989-10-18 Pharming B.V. Dna sequences to target proteins to the mammary gland for efficient secretion
DE3854476T2 (de) 1987-07-06 1996-04-04 Univ Louisiana State Inhibierung von eukaryotischen pathogenen und neoplasmen mit lytischen peptiden.
CA1327311C (en) 1987-07-06 1994-03-01 Jesse M. Jaynes Therapeutic antimicrobial polypeptides, their use and methods for preparation
US5861478A (en) 1987-07-06 1999-01-19 Helix Biomedix, Inc. Lytic peptides
US5212080A (en) 1987-10-05 1993-05-18 Washington University Method of DNA sequencing using DNA transposon Tn5seql
US5137829A (en) 1987-10-05 1992-08-11 Washington University DNA transposon TN5SEQ1
US5162215A (en) 1988-09-22 1992-11-10 Amgen Inc. Method of gene transfer into chickens and other avian species
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5102797A (en) 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
EP0584279B1 (en) 1991-05-14 2001-03-14 The University Of Connecticut Targeted delivery of genes encoding immunogenic proteins
US5753502A (en) 1993-08-05 1998-05-19 Icos Corporation Neuron-specific ICAM-4 promoter
WO1993015185A1 (en) 1992-01-27 1993-08-05 North Carolina State University GENE TRANSFER IN POULTRY BY INTRODUCTION OF EMBRYO CELLS $i(IN OVO)
AU2146092A (en) 1992-05-28 1993-12-30 Scientific Dimensions Usa, Inc. Transgenic animal production with biolistically transformed spermatozoa
EP0669986B1 (en) 1992-11-13 2003-04-09 Idec Pharmaceuticals Corporation Fully impaired consensus kozac sequences for mammalian expression
EP0688358A4 (en) 1993-03-12 1997-10-01 Univ Creighton IMPROVED VECTORS FOR GENTHERAPY
US6607884B1 (en) 1993-03-19 2003-08-19 The Johns Hopkins University School Of Medicine Methods of detecting growth differentiation factor-8
US20030074680A1 (en) 1993-03-19 2003-04-17 Johns Hopkins University School Of Medicine Growth differentiation factor-8
US5645991A (en) 1993-05-04 1997-07-08 Univ. Of Connecticut Transposon-containing DNA cloning vector and uses thereof
US5512483A (en) 1993-05-21 1996-04-30 Mcgill University Expression vectors responsive to steroid hormones
AU7321294A (en) * 1993-06-30 1995-01-24 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College, The Transformed eukaryotic cells, and transposon-based transformation vectors
US6156568A (en) 1993-06-30 2000-12-05 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Transformed eukaryotic cells
WO1995001424A1 (en) 1993-06-30 1995-01-12 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Transformed mammalian cells capable of expressing cecropin b
US5648244A (en) * 1993-09-27 1997-07-15 President And Fellows Of Harvard College Production, purification, cleavage and use of fusion peptides
US5827690A (en) 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
EP0759087B1 (en) 1994-05-13 2001-03-28 Chiron Corporation Compositions and methods for targeting gene delivery vehicles
US5914234A (en) 1994-07-08 1999-06-22 The Johns Hopkins University School Of Medicine Methods of detecting growth differentiation factor-11
US20030167492A1 (en) 1994-07-08 2003-09-04 Johns Hopkins University School Of Medicine Transgenic non-human animals expressing a gdf-11 dominant negative polypeptide, and methods of making and using same
US5843705A (en) 1995-02-21 1998-12-01 Genzyme Transgenic Corporation Transgenically produced antithrombin III
US5998698A (en) 1995-06-07 1999-12-07 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Transgenic fish capable of expressing exogenous lytic peptides
NZ312332A (en) 1995-06-07 2000-01-28 Life Technologies Inc Recombinational cloning using engineered recombination sites
WO1997005266A1 (en) 1995-07-25 1997-02-13 Introgene B.V. Methods and means for targeted gene delivery
GB9518323D0 (en) * 1995-09-07 1995-11-08 Steidler Lothar Materials and methods relating to the attachment and display of substances on cell surfaces
US6218185B1 (en) 1996-04-19 2001-04-17 The United States Of America As Represented By The Secretary Of Agriculture Piggybac transposon-based genetic transformation system for insects
WO1997046589A2 (en) 1996-06-07 1997-12-11 Neorx Corporation Humanized antibodies that bind to the same antigen as bound by antibody nr-lu-13, and their use in pretargeting methods
US6825396B2 (en) 1996-06-12 2004-11-30 Board Of Trustees Operating Michigan State University Methods for tissue specific synthesis of protein in eggs of transgenic hens
CA2259908A1 (en) 1996-07-08 1998-01-15 Dnavec Research Inc. In vivo electroporation method for early animal embryo
WO1998007830A2 (en) 1996-08-22 1998-02-26 The Institute For Genomic Research COMPLETE GENOME SEQUENCE OF THE METHANOGENIC ARCHAEON, $i(METHANOCOCCUS JANNASCHII)
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5925545A (en) 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US6107477A (en) 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences
US6420524B1 (en) 1997-02-20 2002-07-16 Johns Hopkins University School Of Medicine Gain of function mutations in ATP-dependent transposition proteins
AU6184298A (en) 1997-02-25 1998-09-09 Genzyme Transgenics Corporation Transgenically produced non-secreted proteins
AU745049B2 (en) 1997-03-11 2002-03-07 Regents Of The University Of Minnesota DNA-based transposon system for the introduction of nucleic acid into DNA of a cell
US6080912A (en) 1997-03-20 2000-06-27 Wisconsin Alumni Research Foundation Methods for creating transgenic animals
CA2297375A1 (en) 1997-07-24 1999-02-04 Valentis, Inc. Ghrh expression system and methods of use
WO1999005295A1 (en) 1997-07-25 1999-02-04 Thomas Jefferson University Composition and method for targeted integration into cells
US20030115622A1 (en) 1997-08-04 2003-06-19 Ponce De Leon F. Abel Production of avian embryonic germ (eg) cell lines by prolonged culturing of pgc's, use thereof for cloning and chimerization
US6716823B1 (en) 1997-08-13 2004-04-06 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom, and uses thereof
EP1006790A1 (en) 1997-08-22 2000-06-14 Biotechnology and Biological Sciences Research Council Use of mariner transposon in the production of transgenic animals
US6140129A (en) 1997-09-17 2000-10-31 Wisconsin Alumni Research Foundation Chromosomal targeting in bacteria using FLP recombinase
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US7129390B2 (en) 1997-10-16 2006-10-31 Avigenics, Inc Poultry Derived Glycosylated Interferon Alpha 2b
IL135604A0 (en) 1997-10-16 2001-05-20 Univ Georgia Vectors comprising a magnum-specific promoter for avian transgenesis
US20020053092A1 (en) 1997-11-14 2002-05-02 Readhead Carol W. Nucleic acid constructs containing a cyclin A1 promoter, and kit
WO1999025863A1 (en) 1997-11-14 1999-05-27 Cedars-Sinai Medical Center Transfection and transfer of male germ cells for generation of transgenic species
EP0935385A3 (en) * 1998-02-04 2002-06-19 Hitachi, Ltd. Decoder device and receiver using the same
NZ506341A (en) 1998-02-09 2003-02-28 Tranxenogen Inc Genetic manipulation of spermatogonia
IL123411A0 (en) 1998-02-22 1998-09-24 Kimron Veterinary Inst High efficiency methods and compositions for integrating exogenous dna into genomic dna of sperm
US6022716A (en) 1998-04-10 2000-02-08 Genset Sa High throughput DNA sequencing vector
US6291214B1 (en) 1998-05-11 2001-09-18 Glaxo Wellcome Inc. System for generating recombinant viruses
US20020013955A1 (en) 1998-06-10 2002-01-31 Sharon Ogden Production of recombinant protein in transgenic fish
EP1105469B1 (en) 1998-08-11 2010-09-29 University Of Hawaii Mammalian transgenesis by intracytoplasmic sperm injection
AU5690799A (en) 1998-08-25 2000-03-14 Avigenics, Inc. Direct oviduct transgenesis
US20030217375A1 (en) 1998-08-31 2003-11-20 Eyal Zcharia Transgenic animals expressing heparanase and uses thereof
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
US20010036647A1 (en) 1998-10-22 2001-11-01 Prabhakara V. Choudary Functionally assembled antigen-specific intact recombinant antibody and a method for production thereof
EP1375654A3 (en) 1998-11-02 2008-01-16 GTC Biotherapeutics, Inc. Transgenic and cloned mammals
DE69933528T2 (de) 1998-11-09 2007-08-09 Nippon Biologicals, Inc. Verfahren zur Cytokinproduktionn unter Verwendung eines Sendai Virus Expressionssystems
US7160682B2 (en) 1998-11-13 2007-01-09 Regents Of The University Of Minnesota Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell
EP1130960A4 (en) 1998-11-19 2003-07-30 Wisconsin Alumni Res Found TRANSGENIC ANIMALS
WO2000032039A1 (en) 1998-12-04 2000-06-08 Duke University PURIFIED AND ISOLATED piwi FAMILY GENES AND GENE PRODUCTS AND METHODS EMPLOYING SAME
US20020148000A1 (en) 1998-12-04 2002-10-10 Shen Che-Kun James HS-40 enhancer-containing vector in transgenic animals
WO2000056932A2 (en) 1999-03-22 2000-09-28 The University Of Georgia Research Foundation, Inc. Germline-competent avian cells
JP2002542781A (ja) 1999-04-28 2002-12-17 ザ、ボード、オブ、トラスティーズ、オブ、ザ、リーランド、スタンフォード、ジュニア、ユニバーシティ Ｐ因子由来ベクター及びその使用法
US6518481B1 (en) 1999-08-12 2003-02-11 Exelixis, Inc. Universal markers of transgenesis
US6773914B1 (en) 1999-08-19 2004-08-10 The United States Of America As Represented By The Secretary Of Agriculture PiggyBac transformation system
CZ289464B6 (cs) 1999-09-08 2002-01-16 Biopharm Výzkumný Ústav Biofarmacie A Veterinárníc Způsob konstrukce transgenní drůbeľe
KR20020073127A (ko) 1999-09-17 2002-09-19 겐자임 트랜스제닉스 코포레이션 형질전환에 의하여 생성된 융합 단백질
WO2001023525A2 (en) 1999-09-30 2001-04-05 Genzyme Transgenics Corporation Methods of producing cloned and transgenic mammals
US20020055172A1 (en) 1999-10-07 2002-05-09 Harrington John J. Multiple promoter expression constructs and methods of use
HUP0203537A2 (hu) 1999-10-14 2003-02-28 Genzyme Transgenics Corporation Célmolekula transzgénikus állatban történő előállítására és tisztítására szolgáló eljárások
JP4609869B2 (ja) 1999-12-03 2011-01-12 独立行政法人科学技術振興機構 トランスポゾン転移酵素および遺伝子改変方法
NZ519217A (en) 1999-12-10 2004-03-26 Invitrogen Corp Use of multiple recombination sites with unique specificity in recombinational cloning
WO2001044459A2 (en) 1999-12-15 2001-06-21 Regents Of The University Of Minnesota Method to enhance agrobacterium-mediated transformation of plants
AU2103601A (en) 1999-12-17 2001-06-25 Oregon Health And Science University Methods for producing transgenic animals
BR0109269A (pt) 2000-03-17 2002-12-17 Benitec Australia Ltd Construto genético e seu uso, célula de animal vertebrado, métodos de alteração do fenótipo de uma célula de animal vertebrado e de terapia genética em animal vertebrado, animal e animal murino geneticamente modificados
CA2402924A1 (en) 2000-03-21 2001-09-27 Charalambos Savakis Method of generating transgenic organisms using transposons
US20030150007A1 (en) 2000-03-21 2003-08-07 Charalambos Savakis Method of generating transgenic organisms using transposons
US7067308B1 (en) 2000-03-28 2006-06-27 Bioagri Corporation Vector for genetically modifying non-human animals
US6589783B2 (en) 2000-04-13 2003-07-08 Novagen, Inc. Multiple host expression vector
JP4510355B2 (ja) 2000-05-01 2010-07-21 ミッドウエスト リサーチ インスティチュート 安定なチモモナスモビリスのキシロースおよびアラビノース醗酵株
US7354755B2 (en) 2000-05-01 2008-04-08 Midwest Research Institute Stable zymomonas mobilis xylose and arabinose fermenting strains
EP1156114A1 (en) 2000-05-10 2001-11-21 Europäisches Laboratorium für Molekularbiologie Vectors for use in transponson-based DNA sequencing methods
JP2003535122A (ja) 2000-06-02 2003-11-25 ザイコス インク． 生物活性物質のための送達システム
US20020028488A1 (en) 2000-06-19 2002-03-07 Sujay Singh Transgenic avian species for making human and chimeric antibodies
JP2002078491A (ja) 2000-06-22 2002-03-19 National Institute Of Agrobiological Sciences 青枯病菌由来の挿入配列因子
US6852510B2 (en) 2000-07-03 2005-02-08 Gala Design Inc Host cells containing multiple integrating vectors
US20020072097A1 (en) 2000-07-07 2002-06-13 Delcardayre Stephen Molecular breeding of transposable elements
EA007958B1 (ru) 2000-08-03 2007-02-27 Терапеутик Хьюман Поликлоналз Инк. ТРАНСГЕННЫЙ ВЕКТОР, СОДЕРЖАЩИЙ ГУМАНИЗИРОВАННЫЙ ЛОКУС Ig, И ЕГО ПРИМЕНЕНИЕ ДЛЯ ПОЛУЧЕНИЯ ТРАНСГЕННЫХ ЖИВОТНЫХ
GB2368064B (en) 2000-09-30 2002-11-13 Imp Cancer Res Tech DNA Element and Associated Protein
US7105343B1 (en) 2000-10-31 2006-09-12 University Of Notre Dame Du Lac Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector Piggybac
US7067644B2 (en) 2000-12-05 2006-06-27 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
JP2002176880A (ja) 2000-12-12 2002-06-25 Kanegafuchi Chem Ind Co Ltd 効率的な遺伝子導入鳥類の作製法及びそれによって得られる遺伝子導入鳥類
US20020108132A1 (en) 2001-02-02 2002-08-08 Avigenics Inc. Production of a monoclonal antibody by a transgenic chicken
JP2002238559A (ja) * 2001-02-16 2002-08-27 Nagoya Industrial Science Research Inst 鳥類の卵内で外来遺伝子発現産物を生産する方法
US20020119573A1 (en) 2001-02-28 2002-08-29 Shaw Karen J. Footprinting plasmid
EP1478751A4 (en) 2001-03-30 2005-10-19 Avigenics Inc LYSOZYM PROMOTER IN BIRDS
US7176300B2 (en) 2001-03-30 2007-02-13 Avigenics, Inc. Avian lysozyme promoter
JP3723839B2 (ja) 2001-06-07 2005-12-07 国立大学法人広島大学 ニワトリの白血病阻止因子（ｌｉｆ）、及びそれをコードする遺伝子
GB0119497D0 (en) 2001-08-10 2001-10-03 Viragen Inc Expression of modified antibodies in avian cells
EP1451316B1 (en) 2001-09-13 2014-08-06 California Institute Of Technology Method for producing transgenic birds
ATE513038T1 (de) 2001-09-18 2011-07-15 Synageva Biopharma Corp Produktion eines transgenen vogels mittels cytoplasmainjektion
US20030061629A1 (en) 2001-09-21 2003-03-27 Pramod Sutrave Production of transgenic birds using stage X primordial germ cells
AU2002341795A1 (en) 2001-09-21 2003-04-01 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
US20030143740A1 (en) * 2001-10-15 2003-07-31 Christine Wooddell Processes for transposase mediated integration into mammalian cells
EP1446482A4 (en) 2001-10-22 2006-03-01 Athersys Inc COMPOSITIONS AND METHODS FOR GENERATING MUTATIONS IN CELL LINES AND ANIMALS
US7972853B2 (en) 2001-10-22 2011-07-05 Abt Holding Company Compositions and methods for making mutations in cell lines and animals
AU2002365184A1 (en) * 2001-10-26 2003-07-30 Id Biomedical Corporation Of Washington Efficient protein expression system
US6875588B2 (en) 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use
US7294507B2 (en) 2001-11-30 2007-11-13 Avigenics, Inc. Ovomucoid promoters and methods of use
US7335761B2 (en) 2001-11-30 2008-02-26 Avigenics, Inc. Avian gene expression controlling regions
JP2005512598A (ja) 2001-12-21 2005-05-12 オックスフォード バイオメディカ （ユーケー） リミテッド Ｅｉａｖなどのレンチウイルス発現ベクターを使用するトランスジェニック生物の作製方法
US20030121062A1 (en) 2001-12-21 2003-06-26 Oxford Biomedica (Uk) Limited Transgenic organism
US7145057B2 (en) 2002-02-01 2006-12-05 Origen Therapeutics, Inc. Chimeric bird from embryonic stem cells
US7323618B2 (en) 2002-02-01 2008-01-29 Origen Therapeutics, Inc. Tissue specific expression of exogenous proteins in transgenic chickens
US7135562B2 (en) 2002-03-14 2006-11-14 University Of Cincinnati Avian iFABP gene expression controlling region
US20030182675A1 (en) 2002-03-22 2003-09-25 Origen Therapeutics Functional disruption of avian immunoglobulin genes
AU2003291614A1 (en) 2002-05-17 2004-04-08 New Horizons Diagnostics Corporation Identification of a phage associated lytic enzyme to rapidly and specifically detect and kill bacillus anthracis
US20040172667A1 (en) 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US20040235011A1 (en) 2002-06-26 2004-11-25 Cooper Richard K. Production of multimeric proteins
US7527966B2 (en) 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
WO2004009792A2 (en) 2002-07-24 2004-01-29 Vanderbilt University Transposon-based vectors and methods of nucleic acid integration
US7700356B2 (en) 2002-11-08 2010-04-20 The United States Of America As Represented By The Secretary Of Agriculture System for gene targeting and producing stable genomic transgene insertions
GB0227645D0 (en) 2002-11-27 2003-01-08 Viragen Inc Protein production in transgenic avians
WO2004065581A2 (en) 2003-01-15 2004-08-05 Discovery Genomics, Inc. Transposon-insulator element delivery systems
CN1980571A (zh) 2003-01-24 2007-06-13 阿维季尼克斯股份有限公司 禽类及禽蛋中表达的外源蛋白质
EP1594971B1 (en) 2003-02-10 2011-02-02 Max-Delbrück-Centrum für Molekulare Medizin (MDC) Transposon-based targeting system
DK1594973T3 (da) 2003-02-10 2012-02-13 Max Delbrueck Centrum Transposonbaseret målretningssystem
US20050198700A1 (en) 2003-03-07 2005-09-08 Avigenics, Inc. Genomic modification
WO2004080162A2 (en) 2003-03-07 2004-09-23 Avigenics, Inc. Integrase mediated avian transgenesis
US20040255345A1 (en) 2003-03-07 2004-12-16 Rapp Jeffrey C. Production of transgenic avians
US20050034186A1 (en) 2003-03-07 2005-02-10 Harvey Alex J. Site specific nucleic acid integration
US20050273873A1 (en) 2003-03-07 2005-12-08 Avigenics, Inc. Genomic modification
WO2004092351A2 (en) 2003-03-27 2004-10-28 Avigenics, Inc. Production of a transgenic avian by cytoplasmic injection
US7083980B2 (en) 2003-04-17 2006-08-01 Wisconsin Alumni Research Foundation Tn5 transposase mutants and the use thereof
US7381712B2 (en) 2003-05-09 2008-06-03 Avigenics, Inc. In vivo transfection in avians
US20050014932A1 (en) 2003-05-15 2005-01-20 Iogenetics, Llc Targeted biocides
EP1633781A2 (en) 2003-06-06 2006-03-15 Avigenics, Inc. Ovomucoid promoters and methods of use
AU2003252483A1 (en) 2003-07-08 2005-01-21 Japan Science And Technology Corporation Method and system of constructing transgenic organism
US20060218852A1 (en) * 2003-09-12 2006-10-05 Graham David E Controlling the formation of crystalline hydrates in fluid systems
US20080104723A1 (en) 2003-11-21 2008-05-01 Osaka Industrial Promojtion Organization C/O Mydom Development of Mammalian Genome Modification Technique Using Retrotransposon
US8071364B2 (en) 2003-12-24 2011-12-06 Transgenrx, Inc. Gene therapy using transposon-based vectors
US20060123504A1 (en) 2004-12-07 2006-06-08 Avigenics, Inc. Methods of producing polyclonal antibodies
US7569223B2 (en) 2004-03-22 2009-08-04 The Rockefeller University Phage-associated lytic enzymes for treatment of Streptococcus pneumoniae and related conditions
EP1812568A2 (en) 2004-08-25 2007-08-01 Avigenics, Inc. Rna interference in avians
GB0419424D0 (en) 2004-09-02 2004-10-06 Viragen Scotland Ltd Transgene optimisation
US20060160220A1 (en) 2004-11-12 2006-07-20 Iogenetics Retroviral vectors with introns
WO2006055931A2 (en) 2004-11-18 2006-05-26 Stratatech Corporation Vectors for stable gene expression
WO2006055040A2 (en) 2004-11-19 2006-05-26 Government Of The United States Of America, Department Of Health And Human Services Identification of proteins in a genome
EP1819823A2 (en) 2004-12-01 2007-08-22 Bayer Schering Pharma Aktiengesellschaft Generation of replication competent viruses for therapeutic use
US20060153800A1 (en) 2004-12-14 2006-07-13 Roland Buelow DNA immunization with recombinase/transposase
WO2006093847A1 (en) 2005-02-28 2006-09-08 Avigenics, Inc. Artificial chromosomes and transchromosomic avians
US7657816B2 (en) * 2005-07-13 2010-02-02 Leanics Corporation Low-complexity hybrid LDPC code encoder

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