JP2006506970A5 - - Google Patents
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- JP2006506970A5 JP2006506970A5 JP2004521788A JP2004521788A JP2006506970A5 JP 2006506970 A5 JP2006506970 A5 JP 2006506970A5 JP 2004521788 A JP2004521788 A JP 2004521788A JP 2004521788 A JP2004521788 A JP 2004521788A JP 2006506970 A5 JP2006506970 A5 JP 2006506970A5
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- Prior art keywords
- retinal
- cells
- culture system
- cell culture
- cell
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- 238000004113 cell culture Methods 0.000 claims 24
- 210000004027 cell Anatomy 0.000 claims 21
- 210000002569 neuron Anatomy 0.000 claims 21
- 230000002207 retinal effect Effects 0.000 claims 20
- 239000012867 bioactive agent Substances 0.000 claims 12
- 238000000034 method Methods 0.000 claims 10
- 208000017442 Retinal disease Diseases 0.000 claims 8
- 210000001116 retinal neuron Anatomy 0.000 claims 8
- 210000004240 ciliary body Anatomy 0.000 claims 7
- 239000003795 chemical substances by application Substances 0.000 claims 6
- 230000003993 interaction Effects 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 5
- 230000004083 survival effect Effects 0.000 claims 5
- 208000003098 Ganglion Cysts Diseases 0.000 claims 4
- 208000005400 Synovial Cyst Diseases 0.000 claims 4
- 210000000411 amacrine cell Anatomy 0.000 claims 4
- 210000002287 horizontal cell Anatomy 0.000 claims 4
- 230000004770 neurodegeneration Effects 0.000 claims 4
- 238000012258 culturing Methods 0.000 claims 3
- 210000000130 stem cell Anatomy 0.000 claims 3
- 208000024827 Alzheimer disease Diseases 0.000 claims 2
- 206010012689 Diabetic retinopathy Diseases 0.000 claims 2
- 208000010412 Glaucoma Diseases 0.000 claims 2
- 206010038848 Retinal detachment Diseases 0.000 claims 2
- 206010038903 Retinal vascular occlusion Diseases 0.000 claims 2
- 208000007014 Retinitis pigmentosa Diseases 0.000 claims 2
- 210000005158 embryonic retinal progenitor cell Anatomy 0.000 claims 2
- 230000002708 enhancing effect Effects 0.000 claims 2
- 208000002780 macular degeneration Diseases 0.000 claims 2
- 210000000608 photoreceptor cell Anatomy 0.000 claims 2
- 108091008695 photoreceptors Proteins 0.000 claims 2
- 230000004264 retinal detachment Effects 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
Claims (21)
- 成熟な神経細胞および毛様体から単離された細胞の混合物を含む、細胞培養系。
- 前記成熟な神経細胞が成熟な網膜神経細胞を含む、請求項１に記載の細胞培養系。
- 前記成熟な網膜神経細胞が、双極細胞、水平細胞、アマクリン細胞、神経節細胞および光受容細胞からなる群から選択される、請求項２に記載の細胞培養系。
- 成熟な網膜神経細胞および毛様体から単離された細胞の混合物を含む、網膜細胞培養系。
- 前記成熟な網膜神経細胞が、双極細胞、水平細胞、アマクリン細胞、神経節細胞および光受容体からなる群から選択される、請求項４に記載の網膜細胞培養系。
- 成熟な網膜神経細胞および毛様体から単離された細胞の混合物を含む網膜細胞培養系であって、該成熟な網膜神経細胞は、双極細胞、水平細胞、アマクリン細胞、神経節細胞および光受容細胞からなる群から選択される、網膜細胞培養系。
- （ｉ）成熟な網膜神経細胞；（ｉｉ）毛様体から単離された細胞；および（ｉｉｉ）胚性網膜細胞の混合物を含む、網膜細胞培養系。
- 前記胚性網膜細胞が網膜幹細胞を含む、請求項７に記載の細胞培養系。
- 前記胚性網膜細胞が胚性網膜前駆細胞を含む、請求項７に記載の細胞培養系。
- 前記成熟な網膜神経細胞が、双極細胞、水平細胞、アマクリン細胞、神経節細胞および光受容細胞からなる群から選択される、請求項７に記載の細胞培養系。
- 成熟な網膜神経細胞と、毛様体から単離された細胞とを同時に培養する工程を包含する、網膜細胞培養系を生成するための方法。
- 成熟な網膜神経細胞と、毛様体から単離された細胞とを同時に培養する工程を包含する、インビトロにおいて成熟な網膜神経細胞の生存を増強するための方法。
- （ｉ）成熟な網膜神経細胞；（ｉｉ）毛様体から単離された細胞；および（ｉｉｉ）胚性網膜細胞を同時に培養する工程を包含する、請求項１１または請求項１２のいずれかに記載の方法。
- 前記胚性網膜細胞が、網膜幹細胞および胚性網膜前駆細胞からなる群から選択される、請求項１３に記載の方法。
- 神経細胞の生存を増強し得る生物活性薬剤を同定するための方法であって、（ｉ）請求項１〜１０のいずれか１項に記載の細胞培養系の神経細胞と、候補薬剤との間の相互作用を許容する条件下で、かつ、該相互作用を許容するのに十分な時間、該候補薬剤と該細胞培養系とを接触させる工程；および（ｉｉ）該候補薬剤の存在下での該細胞培養系の神経細胞の生存と、該候補薬剤の不在下での該細胞培養系の神経細胞の生存とを比較し、そこから、該神経細胞の生存を増強し得る生物活性薬剤を同定する工程、を包含する、方法。
- 神経細胞の神経変性を阻害し得る生物活性薬剤を同定するための方法であって、（ｉ）請求項１〜１０のいずれか１項に記載の細胞培養系の神経細胞と候補薬剤との間の相互作用を許容にする条件下で、かつ、該相互作用を許容するのに十分な時間、該生物活性薬剤と該細胞培養系とを接触させる工程；および（ｉｉ）該生物活性薬剤の存在下での該細胞培養系の神経細胞の構造と、該生物活性薬剤の不在下での該細胞培養系の神経細胞の構造とを比較し、そこから、該神経細胞の神経変性を阻害し得る生物活性薬剤を同定する工程、を包含する、方法。
- 網膜疾患を処置し得る生物活性薬剤を同定するための方法であって、請求項１〜１０のいずれか１項に記載の細胞培養系の神経細胞と候補薬剤との間の相互作用を許容する条件下で、かつ、該相互作用を許容するのに十分な時間、該生物活性薬剤と該細胞培養系とを接触させる工程；および（ｉｉ）該生物活性薬剤の存在下での該細胞培養系の神経細胞の神経変性と、該生物活性薬剤の不在下での該細胞培養系の神経細胞の神経変性とを比較し、そこから、網膜疾患を処置し得る生物活性薬剤を同定する工程、を包含する、方法。
- 前記神経細胞が網膜神経細胞である、請求項１５〜１７のいずれか１項に記載の方法。
- 前記網膜疾患が、黄斑変性症、緑内障、糖尿病性網膜症、網膜剥離、網膜血管閉塞、網膜色素変性症およびアルツハイマー病に関連する網膜障害からなる群から選択される、請求項１７に記載の方法。
- 網膜疾患の処置を必要とする被験体において、該被験体の網膜組織に導入するのに適切な単離された網膜幹細胞を含む、網膜疾患を処置するための組成物。
- 前記網膜疾患が、黄斑変性症、緑内障、糖尿病性網膜症、網膜剥離、網膜血管閉塞、網膜色素変性症およびアルツハイマー病に関連する網膜障害からなる群から選択される、請求項２０に記載の組成物。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39597302P | 2002-07-12 | 2002-07-12 | |
| PCT/US2003/021968 WO2004007749A2 (en) | 2002-07-12 | 2003-07-11 | Methods and systems for extended in vitro culture of neuronal cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006506970A JP2006506970A (ja) | 2006-03-02 |
| JP2006506970A5 true JP2006506970A5 (ja) | 2006-08-31 |
Family
ID=30115948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004521788A Ceased JP2006506970A (ja) | 2002-07-12 | 2003-07-11 | 神経細胞の延長されたインビトロ培養のための方法および系 |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US7312025B2 (ja) |
| EP (1) | EP1551956A4 (ja) |
| JP (1) | JP2006506970A (ja) |
| AU (1) | AU2003249227A1 (ja) |
| CA (1) | CA2493849A1 (ja) |
| WO (1) | WO2004007749A2 (ja) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1551956A4 (en) * | 2002-07-12 | 2006-12-27 | Univ Washington | METHOD AND SYSTEMS FOR THE EXTENDED IN VITRO CULTURE OF NEURONAL CELLS |
| JP2007500509A (ja) * | 2003-07-30 | 2007-01-18 | アクセラ， インコーポレイテッド | 長期一次網膜細胞培養およびストレスモデル、ならびにこれらの使用方法 |
| WO2005056174A2 (en) * | 2003-12-02 | 2005-06-23 | Ppg Industries Ohio, Inc. | Colloidal particle sols and methods for preparing the same |
| US7541186B2 (en) | 2006-02-22 | 2009-06-02 | University Of Washington | Method of generating human retinal progenitors from embryonic stem cells |
| US9061017B2 (en) | 2006-05-03 | 2015-06-23 | The Schepens Eye Research Institute, Inc. | Isolation and therapeutic application of adult retinal stem cells collected from extra-retinal tissues |
| WO2007130060A2 (en) | 2006-05-03 | 2007-11-15 | Schepens Eye Research | Isolation and therapeutic application of adult retinal stem cells collected from extra-retinal tissues |
| RU2494089C2 (ru) | 2007-04-20 | 2013-09-27 | Акьюсела Инк. | Соединения, представляющие собой стиролильные производные, для лечения офтальмических заболеваний и расстройств |
| CN101784188B (zh) | 2007-06-29 | 2014-02-12 | 奥克塞拉有限公司 | 用于治疗眼科疾病和紊乱的炔基苯基衍生化合物 |
| EP3210966B1 (en) | 2007-10-05 | 2020-01-08 | Acucela, Inc. | Alkoxyphenylpropylamines for the treatment of age-related macular degeneration |
| WO2010101587A1 (en) * | 2009-03-04 | 2010-09-10 | Children's Medical Center Corporation | Use of hypoxanthine for promotion of neuronal outgrowth |
| WO2011127616A1 (en) * | 2010-04-13 | 2011-10-20 | M-Lab Ag | Diagnostic methods for glaucoma |
| KR20140147805A (ko) * | 2011-12-07 | 2014-12-30 | 아바스템 바이오메디컬 코퍼레이션 | 지방조직으로부터 비지방 세포의 단리를 위한 방법 및 장치 |
| WO2013109991A1 (en) | 2012-01-20 | 2013-07-25 | Acucela Inc. | Substituted heterocyclic compounds for disease treatment |
| CA2903483A1 (en) | 2013-03-12 | 2014-10-09 | Acucela Inc. | Substituted 3-phenylpropylamine derivatives for the treatment of ophthalmic diseases and disorders |
| US11241460B2 (en) | 2013-03-15 | 2022-02-08 | Astellas Institute For Regenerative Medicine | Photoreceptors and photoreceptor progenitors produced from pluripotent stem cells |
| WO2015107738A1 (ja) | 2014-01-17 | 2015-07-23 | 住友化学株式会社 | 毛様体周縁部幹細胞の製造方法 |
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-
2003
- 2003-07-11 EP EP03764619A patent/EP1551956A4/en not_active Withdrawn
- 2003-07-11 CA CA002493849A patent/CA2493849A1/en not_active Abandoned
- 2003-07-11 US US10/618,076 patent/US7312025B2/en not_active Expired - Fee Related
- 2003-07-11 WO PCT/US2003/021968 patent/WO2004007749A2/en active Search and Examination
- 2003-07-11 JP JP2004521788A patent/JP2006506970A/ja not_active Ceased
- 2003-07-11 AU AU2003249227A patent/AU2003249227A1/en not_active Abandoned
-
2007
- 2007-11-16 US US11/985,856 patent/US20080175826A1/en not_active Abandoned
-
2010
- 2010-08-23 US US12/861,754 patent/US20110044957A1/en not_active Abandoned
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