JP2005532042A5 - - Google Patents
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- JP2005532042A5 JP2005532042A5 JP2003580487A JP2003580487A JP2005532042A5 JP 2005532042 A5 JP2005532042 A5 JP 2005532042A5 JP 2003580487 A JP2003580487 A JP 2003580487A JP 2003580487 A JP2003580487 A JP 2003580487A JP 2005532042 A5 JP2005532042 A5 JP 2005532042A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- template
- amplification product
- gene
- competitive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 30
- 108020004707 nucleic acids Proteins 0.000 claims 23
- 150000007523 nucleic acids Chemical class 0.000 claims 23
- 102000039446 nucleic acids Human genes 0.000 claims 23
- 230000002860 competitive effect Effects 0.000 claims 17
- 108090000623 proteins and genes Proteins 0.000 claims 14
- 230000003321 amplification Effects 0.000 claims 12
- 238000003199 nucleic acid amplification method Methods 0.000 claims 12
- 239000000203 mixture Substances 0.000 claims 8
- 108091034117 Oligonucleotide Proteins 0.000 claims 5
- 230000014509 gene expression Effects 0.000 claims 5
- 239000002299 complementary DNA Substances 0.000 claims 4
- 238000007865 diluting Methods 0.000 claims 3
- 108700039887 Essential Genes Proteins 0.000 claims 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
- 238000003556 assay Methods 0.000 claims 2
- 102000007469 Actins Human genes 0.000 claims 1
- 108010085238 Actins Proteins 0.000 claims 1
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims 1
- 108091081021 Sense strand Proteins 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 238000002966 oligonucleotide array Methods 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/109,349 US20030186246A1 (en) | 2002-03-28 | 2002-03-28 | Multiplex standardized reverse transcriptase-polymerase chain reacton method for assessment of gene expression in small biological samples |
| PCT/US2003/008657 WO2003083051A2 (en) | 2002-03-28 | 2003-03-20 | Multiplex standardized reverse transcriptase-polymerase chain reaction method for assessment of gene expression in small biological samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005532042A JP2005532042A (ja) | 2005-10-27 |
| JP2005532042A5 true JP2005532042A5 (https=) | 2006-05-11 |
Family
ID=28453085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003580487A Pending JP2005532042A (ja) | 2002-03-28 | 2003-03-20 | 少量の生体試料における遺伝子発現の評価のためのマルチプレックス標準化逆転写酵素‐ポリメラーゼ連鎖反応法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20030186246A1 (https=) |
| EP (1) | EP1487987A4 (https=) |
| JP (1) | JP2005532042A (https=) |
| AU (1) | AU2003222031A1 (https=) |
| CA (1) | CA2480160A1 (https=) |
| WO (1) | WO2003083051A2 (https=) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7087414B2 (en) | 2000-06-06 | 2006-08-08 | Applera Corporation | Methods and devices for multiplexing amplification reactions |
| US20040197785A1 (en) * | 2001-03-14 | 2004-10-07 | Willey James C. | Method for quantitative measurement of gene expression for indentifying individuals at risk for bronchogenic carcinoma |
| JP2006506945A (ja) * | 2002-03-28 | 2006-03-02 | メディカル・カレッジ・オブ・オハイオ | 遺伝子発現プロフィールを使用する非小細胞肺癌の診断および処置のための方法および組成物 |
| EP2031070B1 (en) | 2002-12-04 | 2013-07-17 | Life Technologies Corporation | Multiplex amplification of polynucleotides |
| US20050095600A1 (en) * | 2003-10-31 | 2005-05-05 | Xiang Yu | Methods of generating gene-specific probes for nucleic acid array detection |
| WO2005086679A2 (en) * | 2004-03-05 | 2005-09-22 | Medical College Of Ohio | Methods and compositions for assessing nucleic acids and alleles |
| WO2005106036A2 (en) * | 2004-04-12 | 2005-11-10 | Medical College Of Ohio | Methods and compositions for assaying analytes |
| JP2008514900A (ja) | 2004-07-30 | 2008-05-08 | アデザ・バイオメデイカル・コーポレイシヨン | 疾病および他の状態のマーカーとしての癌胎児性フィブロネクチンならびに癌胎児性フィブロネクチンの検出のための方法 |
| WO2006079048A2 (en) * | 2005-01-21 | 2006-07-27 | Gene Express, Inc. | Databases for assessing nucleic acids |
| ATE548467T1 (de) * | 2005-09-02 | 2012-03-15 | Univ Toledo | Verfahren zur identifizierung von biomarkern mit eignung bei der diagnose biologischer zustände |
| WO2009039190A1 (en) * | 2007-09-17 | 2009-03-26 | Gene Express, Inc. | Cancer risk biomarker |
| WO2010045462A1 (en) * | 2008-10-15 | 2010-04-22 | Biotrove, Inc. | System for identification of multiple nucleic acid targets in a single sample and use thereof |
| WO2014063051A2 (en) | 2012-10-18 | 2014-04-24 | Idexx Laboratories, Inc. | Nucleic acid amplification controls and kits and methods of use thereof |
| CN103952484B (zh) * | 2014-04-23 | 2016-05-04 | 深圳市慢性病防治中心 | 一种基于qPCR分析大鼠硒蛋白基因表达谱的方法 |
| US11655510B2 (en) | 2015-04-20 | 2023-05-23 | Cellecta, Inc. | Experimentally validated sets of gene specific primers for use in multiplex applications |
| US10975440B2 (en) * | 2015-04-20 | 2021-04-13 | Cellecta, Inc. | Experimentally validated sets of gene specific primers for use in multiplex applications |
| WO2020036926A1 (en) | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| CN110452959B (zh) * | 2019-07-01 | 2023-06-16 | 昆明理工大学 | 一种黄草乌实时定量pcr内参基因的筛选方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| EP0391232B1 (en) * | 1989-04-05 | 1994-06-08 | Central Glass Company, Limited | Fluoroolefin-alkene base copolymer useful as paint vehicle |
| US5876978A (en) * | 1993-04-06 | 1999-03-02 | Medical College Of Ohio | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| US5643765A (en) * | 1993-04-06 | 1997-07-01 | University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| US5639606A (en) * | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| US5639600A (en) * | 1994-08-05 | 1997-06-17 | The Regents Of The University Of California | Diagnosis and treatment of cell proliferative disease having clonal macrophage involvement |
| JP2869006B2 (ja) * | 1994-10-13 | 1999-03-10 | インターナショナル・ビジネス・マシーンズ・コーポレイション | 映像信号処理装置及び映像信号処理方法 |
| US6605451B1 (en) * | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
| US20040197785A1 (en) * | 2001-03-14 | 2004-10-07 | Willey James C. | Method for quantitative measurement of gene expression for indentifying individuals at risk for bronchogenic carcinoma |
| JP2006506945A (ja) * | 2002-03-28 | 2006-03-02 | メディカル・カレッジ・オブ・オハイオ | 遺伝子発現プロフィールを使用する非小細胞肺癌の診断および処置のための方法および組成物 |
| WO2005086679A2 (en) * | 2004-03-05 | 2005-09-22 | Medical College Of Ohio | Methods and compositions for assessing nucleic acids and alleles |
| WO2005106036A2 (en) * | 2004-04-12 | 2005-11-10 | Medical College Of Ohio | Methods and compositions for assaying analytes |
| WO2006079048A2 (en) * | 2005-01-21 | 2006-07-27 | Gene Express, Inc. | Databases for assessing nucleic acids |
| ATE548467T1 (de) * | 2005-09-02 | 2012-03-15 | Univ Toledo | Verfahren zur identifizierung von biomarkern mit eignung bei der diagnose biologischer zustände |
| WO2009039190A1 (en) * | 2007-09-17 | 2009-03-26 | Gene Express, Inc. | Cancer risk biomarker |
-
2002
- 2002-03-28 US US10/109,349 patent/US20030186246A1/en not_active Abandoned
-
2003
- 2003-03-20 AU AU2003222031A patent/AU2003222031A1/en not_active Abandoned
- 2003-03-20 JP JP2003580487A patent/JP2005532042A/ja active Pending
- 2003-03-20 CA CA002480160A patent/CA2480160A1/en not_active Abandoned
- 2003-03-20 EP EP03718015A patent/EP1487987A4/en not_active Ceased
- 2003-03-20 WO PCT/US2003/008657 patent/WO2003083051A2/en not_active Ceased
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