JP2005532042A - 少量の生体試料における遺伝子発現の評価のためのマルチプレックス標準化逆転写酵素‐ポリメラーゼ連鎖反応法 - Google Patents

少量の生体試料における遺伝子発現の評価のためのマルチプレックス標準化逆転写酵素‐ポリメラーゼ連鎖反応法 Download PDF

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JP2005532042A
JP2005532042A JP2003580487A JP2003580487A JP2005532042A JP 2005532042 A JP2005532042 A JP 2005532042A JP 2003580487 A JP2003580487 A JP 2003580487A JP 2003580487 A JP2003580487 A JP 2003580487A JP 2005532042 A JP2005532042 A JP 2005532042A
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gene
pcr
cdna
gene expression
target gene
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ウィリー,ジェイムズ・シー
クロフォード,エリン・エル
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メディカル・カレッジ・オブ・オハイオ
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification

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  • Chemical & Material Sciences (AREA)
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JP2003580487A 2002-03-28 2003-03-20 少量の生体試料における遺伝子発現の評価のためのマルチプレックス標準化逆転写酵素‐ポリメラーゼ連鎖反応法 Pending JP2005532042A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/109,349 US20030186246A1 (en) 2002-03-28 2002-03-28 Multiplex standardized reverse transcriptase-polymerase chain reacton method for assessment of gene expression in small biological samples
PCT/US2003/008657 WO2003083051A2 (en) 2002-03-28 2003-03-20 Multiplex standardized reverse transcriptase-polymerase chain reaction method for assessment of gene expression in small biological samples

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JP2005532042A true JP2005532042A (ja) 2005-10-27
JP2005532042A5 JP2005532042A5 (https=) 2006-05-11

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JP2003580487A Pending JP2005532042A (ja) 2002-03-28 2003-03-20 少量の生体試料における遺伝子発現の評価のためのマルチプレックス標準化逆転写酵素‐ポリメラーゼ連鎖反応法

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US (1) US20030186246A1 (https=)
EP (1) EP1487987A4 (https=)
JP (1) JP2005532042A (https=)
AU (1) AU2003222031A1 (https=)
CA (1) CA2480160A1 (https=)
WO (1) WO2003083051A2 (https=)

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* Cited by examiner, † Cited by third party
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US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
US20040197785A1 (en) * 2001-03-14 2004-10-07 Willey James C. Method for quantitative measurement of gene expression for indentifying individuals at risk for bronchogenic carcinoma
JP2006506945A (ja) * 2002-03-28 2006-03-02 メディカル・カレッジ・オブ・オハイオ 遺伝子発現プロフィールを使用する非小細胞肺癌の診断および処置のための方法および組成物
EP2031070B1 (en) 2002-12-04 2013-07-17 Life Technologies Corporation Multiplex amplification of polynucleotides
US20050095600A1 (en) * 2003-10-31 2005-05-05 Xiang Yu Methods of generating gene-specific probes for nucleic acid array detection
WO2005086679A2 (en) * 2004-03-05 2005-09-22 Medical College Of Ohio Methods and compositions for assessing nucleic acids and alleles
WO2005106036A2 (en) * 2004-04-12 2005-11-10 Medical College Of Ohio Methods and compositions for assaying analytes
JP2008514900A (ja) 2004-07-30 2008-05-08 アデザ・バイオメデイカル・コーポレイシヨン 疾病および他の状態のマーカーとしての癌胎児性フィブロネクチンならびに癌胎児性フィブロネクチンの検出のための方法
WO2006079048A2 (en) * 2005-01-21 2006-07-27 Gene Express, Inc. Databases for assessing nucleic acids
ATE548467T1 (de) * 2005-09-02 2012-03-15 Univ Toledo Verfahren zur identifizierung von biomarkern mit eignung bei der diagnose biologischer zustände
WO2009039190A1 (en) * 2007-09-17 2009-03-26 Gene Express, Inc. Cancer risk biomarker
WO2010045462A1 (en) * 2008-10-15 2010-04-22 Biotrove, Inc. System for identification of multiple nucleic acid targets in a single sample and use thereof
WO2014063051A2 (en) 2012-10-18 2014-04-24 Idexx Laboratories, Inc. Nucleic acid amplification controls and kits and methods of use thereof
CN103952484B (zh) * 2014-04-23 2016-05-04 深圳市慢性病防治中心 一种基于qPCR分析大鼠硒蛋白基因表达谱的方法
US11655510B2 (en) 2015-04-20 2023-05-23 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
US10975440B2 (en) * 2015-04-20 2021-04-13 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
WO2020036926A1 (en) 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
CN110452959B (zh) * 2019-07-01 2023-06-16 昆明理工大学 一种黄草乌实时定量pcr内参基因的筛选方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0391232B1 (en) * 1989-04-05 1994-06-08 Central Glass Company, Limited Fluoroolefin-alkene base copolymer useful as paint vehicle
US5876978A (en) * 1993-04-06 1999-03-02 Medical College Of Ohio Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5643765A (en) * 1993-04-06 1997-07-01 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5639606A (en) * 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5639600A (en) * 1994-08-05 1997-06-17 The Regents Of The University Of California Diagnosis and treatment of cell proliferative disease having clonal macrophage involvement
JP2869006B2 (ja) * 1994-10-13 1999-03-10 インターナショナル・ビジネス・マシーンズ・コーポレイション 映像信号処理装置及び映像信号処理方法
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US20040197785A1 (en) * 2001-03-14 2004-10-07 Willey James C. Method for quantitative measurement of gene expression for indentifying individuals at risk for bronchogenic carcinoma
JP2006506945A (ja) * 2002-03-28 2006-03-02 メディカル・カレッジ・オブ・オハイオ 遺伝子発現プロフィールを使用する非小細胞肺癌の診断および処置のための方法および組成物
WO2005086679A2 (en) * 2004-03-05 2005-09-22 Medical College Of Ohio Methods and compositions for assessing nucleic acids and alleles
WO2005106036A2 (en) * 2004-04-12 2005-11-10 Medical College Of Ohio Methods and compositions for assaying analytes
WO2006079048A2 (en) * 2005-01-21 2006-07-27 Gene Express, Inc. Databases for assessing nucleic acids
ATE548467T1 (de) * 2005-09-02 2012-03-15 Univ Toledo Verfahren zur identifizierung von biomarkern mit eignung bei der diagnose biologischer zustände
WO2009039190A1 (en) * 2007-09-17 2009-03-26 Gene Express, Inc. Cancer risk biomarker

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Publication number Publication date
AU2003222031A8 (en) 2003-10-13
WO2003083051A3 (en) 2004-03-25
AU2003222031A1 (en) 2003-10-13
WO2003083051A2 (en) 2003-10-09
EP1487987A4 (en) 2006-04-05
CA2480160A1 (en) 2003-10-09
US20030186246A1 (en) 2003-10-02
EP1487987A2 (en) 2004-12-22

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