AU2003222031A1 - Multiplex standardized reverse transcriptase-polymerase chain reaction method for assessment of gene expression in small biological samples - Google Patents

Multiplex standardized reverse transcriptase-polymerase chain reaction method for assessment of gene expression in small biological samples

Info

Publication number
AU2003222031A1
AU2003222031A1 AU2003222031A AU2003222031A AU2003222031A1 AU 2003222031 A1 AU2003222031 A1 AU 2003222031A1 AU 2003222031 A AU2003222031 A AU 2003222031A AU 2003222031 A AU2003222031 A AU 2003222031A AU 2003222031 A1 AU2003222031 A1 AU 2003222031A1
Authority
AU
Australia
Prior art keywords
assessment
gene expression
chain reaction
polymerase chain
reverse transcriptase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003222031A
Other languages
English (en)
Other versions
AU2003222031A8 (en
Inventor
Erin L. Crawford
James C. Willey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical College of Ohio
Original Assignee
Medical College of Ohio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical College of Ohio filed Critical Medical College of Ohio
Publication of AU2003222031A8 publication Critical patent/AU2003222031A8/xx
Publication of AU2003222031A1 publication Critical patent/AU2003222031A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AU2003222031A 2002-03-28 2003-03-20 Multiplex standardized reverse transcriptase-polymerase chain reaction method for assessment of gene expression in small biological samples Abandoned AU2003222031A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/109,349 2002-03-28
US10/109,349 US20030186246A1 (en) 2002-03-28 2002-03-28 Multiplex standardized reverse transcriptase-polymerase chain reacton method for assessment of gene expression in small biological samples
PCT/US2003/008657 WO2003083051A2 (en) 2002-03-28 2003-03-20 Multiplex standardized reverse transcriptase-polymerase chain reaction method for assessment of gene expression in small biological samples

Publications (2)

Publication Number Publication Date
AU2003222031A8 AU2003222031A8 (en) 2003-10-13
AU2003222031A1 true AU2003222031A1 (en) 2003-10-13

Family

ID=28453085

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003222031A Abandoned AU2003222031A1 (en) 2002-03-28 2003-03-20 Multiplex standardized reverse transcriptase-polymerase chain reaction method for assessment of gene expression in small biological samples

Country Status (6)

Country Link
US (1) US20030186246A1 (https=)
EP (1) EP1487987A4 (https=)
JP (1) JP2005532042A (https=)
AU (1) AU2003222031A1 (https=)
CA (1) CA2480160A1 (https=)
WO (1) WO2003083051A2 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
US20040197785A1 (en) * 2001-03-14 2004-10-07 Willey James C. Method for quantitative measurement of gene expression for indentifying individuals at risk for bronchogenic carcinoma
JP2006506945A (ja) * 2002-03-28 2006-03-02 メディカル・カレッジ・オブ・オハイオ 遺伝子発現プロフィールを使用する非小細胞肺癌の診断および処置のための方法および組成物
EP2031070B1 (en) 2002-12-04 2013-07-17 Life Technologies Corporation Multiplex amplification of polynucleotides
US20050095600A1 (en) * 2003-10-31 2005-05-05 Xiang Yu Methods of generating gene-specific probes for nucleic acid array detection
WO2005086679A2 (en) * 2004-03-05 2005-09-22 Medical College Of Ohio Methods and compositions for assessing nucleic acids and alleles
WO2005106036A2 (en) * 2004-04-12 2005-11-10 Medical College Of Ohio Methods and compositions for assaying analytes
JP2008514900A (ja) 2004-07-30 2008-05-08 アデザ・バイオメデイカル・コーポレイシヨン 疾病および他の状態のマーカーとしての癌胎児性フィブロネクチンならびに癌胎児性フィブロネクチンの検出のための方法
WO2006079048A2 (en) * 2005-01-21 2006-07-27 Gene Express, Inc. Databases for assessing nucleic acids
ATE548467T1 (de) * 2005-09-02 2012-03-15 Univ Toledo Verfahren zur identifizierung von biomarkern mit eignung bei der diagnose biologischer zustände
WO2009039190A1 (en) * 2007-09-17 2009-03-26 Gene Express, Inc. Cancer risk biomarker
WO2010045462A1 (en) * 2008-10-15 2010-04-22 Biotrove, Inc. System for identification of multiple nucleic acid targets in a single sample and use thereof
WO2014063051A2 (en) 2012-10-18 2014-04-24 Idexx Laboratories, Inc. Nucleic acid amplification controls and kits and methods of use thereof
CN103952484B (zh) * 2014-04-23 2016-05-04 深圳市慢性病防治中心 一种基于qPCR分析大鼠硒蛋白基因表达谱的方法
US11655510B2 (en) 2015-04-20 2023-05-23 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
US10975440B2 (en) * 2015-04-20 2021-04-13 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
WO2020036926A1 (en) 2018-08-17 2020-02-20 Cellecta, Inc. Multiplex preparation of barcoded gene specific dna fragments
CN110452959B (zh) * 2019-07-01 2023-06-16 昆明理工大学 一种黄草乌实时定量pcr内参基因的筛选方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0391232B1 (en) * 1989-04-05 1994-06-08 Central Glass Company, Limited Fluoroolefin-alkene base copolymer useful as paint vehicle
US5876978A (en) * 1993-04-06 1999-03-02 Medical College Of Ohio Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5643765A (en) * 1993-04-06 1997-07-01 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5639606A (en) * 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5639600A (en) * 1994-08-05 1997-06-17 The Regents Of The University Of California Diagnosis and treatment of cell proliferative disease having clonal macrophage involvement
JP2869006B2 (ja) * 1994-10-13 1999-03-10 インターナショナル・ビジネス・マシーンズ・コーポレイション 映像信号処理装置及び映像信号処理方法
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US20040197785A1 (en) * 2001-03-14 2004-10-07 Willey James C. Method for quantitative measurement of gene expression for indentifying individuals at risk for bronchogenic carcinoma
JP2006506945A (ja) * 2002-03-28 2006-03-02 メディカル・カレッジ・オブ・オハイオ 遺伝子発現プロフィールを使用する非小細胞肺癌の診断および処置のための方法および組成物
WO2005086679A2 (en) * 2004-03-05 2005-09-22 Medical College Of Ohio Methods and compositions for assessing nucleic acids and alleles
WO2005106036A2 (en) * 2004-04-12 2005-11-10 Medical College Of Ohio Methods and compositions for assaying analytes
WO2006079048A2 (en) * 2005-01-21 2006-07-27 Gene Express, Inc. Databases for assessing nucleic acids
ATE548467T1 (de) * 2005-09-02 2012-03-15 Univ Toledo Verfahren zur identifizierung von biomarkern mit eignung bei der diagnose biologischer zustände
WO2009039190A1 (en) * 2007-09-17 2009-03-26 Gene Express, Inc. Cancer risk biomarker

Also Published As

Publication number Publication date
JP2005532042A (ja) 2005-10-27
AU2003222031A8 (en) 2003-10-13
WO2003083051A3 (en) 2004-03-25
WO2003083051A2 (en) 2003-10-09
EP1487987A4 (en) 2006-04-05
CA2480160A1 (en) 2003-10-09
US20030186246A1 (en) 2003-10-02
EP1487987A2 (en) 2004-12-22

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase