JP2004525624A - 細胞コレクションのライブラリー - Google Patents

細胞コレクションのライブラリー Download PDF

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Publication number
JP2004525624A
JP2004525624A JP2002559584A JP2002559584A JP2004525624A JP 2004525624 A JP2004525624 A JP 2004525624A JP 2002559584 A JP2002559584 A JP 2002559584A JP 2002559584 A JP2002559584 A JP 2002559584A JP 2004525624 A JP2004525624 A JP 2004525624A
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library
cells
cell
bases
promoter
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JP2004525624A5 (enExample
Inventor
ゴールドスミス,ニール
エム.ピー.サンタナ セレンセン,アレクサンドラ
ベー.エス. ニエルセン,セレン
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エボルバ バイオテック アクティーゼルスカブ
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Eye Examination Apparatus (AREA)
  • Holo Graphy (AREA)
  • Aerials With Secondary Devices (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2002559584A 2001-01-25 2002-01-25 細胞コレクションのライブラリー Withdrawn JP2004525624A (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200100128 2001-01-25
DKPA200100679 2001-05-01
US30086301P 2001-06-27 2001-06-27
PCT/DK2002/000056 WO2002059297A2 (en) 2001-01-25 2002-01-25 A library of a collection of cells

Related Child Applications (1)

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JP2009233636A Division JP4965625B2 (ja) 2001-01-25 2009-10-07 細胞コレクションのライブラリー

Publications (2)

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JP2004525624A true JP2004525624A (ja) 2004-08-26
JP2004525624A5 JP2004525624A5 (enExample) 2005-12-22

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JP2002559584A Withdrawn JP2004525624A (ja) 2001-01-25 2002-01-25 細胞コレクションのライブラリー
JP2009233636A Expired - Fee Related JP4965625B2 (ja) 2001-01-25 2009-10-07 細胞コレクションのライブラリー

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US (2) US7838287B2 (enExample)
EP (1) EP1356037B1 (enExample)
JP (2) JP2004525624A (enExample)
AT (1) ATE501251T1 (enExample)
AU (1) AU2002227883A1 (enExample)
CA (1) CA2474172C (enExample)
DE (1) DE60239386D1 (enExample)
DK (1) DK1356037T3 (enExample)
WO (1) WO2002059297A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7212982B1 (ja) 2022-10-05 2023-01-26 株式会社Logomix 細胞ライブラリおよびその製造方法
WO2024075756A1 (ja) * 2022-10-05 2024-04-11 株式会社Logomix 細胞ライブラリおよびその製造方法

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
WO2002059297A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
US20050164162A1 (en) * 2002-01-25 2005-07-28 Evolva Ltd., C/O Dr. Iur. Martin Eisenring Methods for multiple parameters screening and evolution of cells to produce small molecules with multiple functionalities
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
NZ537277A (en) 2002-07-18 2008-04-30 Crucell Holland Bv Recombinant production of mixtures of antibodies
DK1529110T3 (da) * 2002-08-01 2012-01-30 Evolva Ltd Fremgangsmåde til blanding af store antal af heterologe gener
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
EP1737971B1 (en) * 2004-01-20 2017-08-16 Merus N.V. Mixtures of binding proteins
AU2006227165B2 (en) 2005-03-18 2011-11-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
BRPI0923225A2 (pt) 2008-12-02 2016-10-04 Chiralgen Ltd metodo para sintese de acidos nucleicos modificados no atomo de fosforo
KR101153866B1 (ko) 2009-05-21 2012-06-27 대한민국 형질전환용 운반체, 이의 제조방법, 이를 이용한 형질전환방법 및 형질전환체
EP2451461A4 (en) 2009-07-06 2013-05-29 Ontorii Inc NOVEL NUCLEIC ACID PRODRUGS AND METHOD OF USE THEREOF
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
CN105671108A (zh) 2010-06-02 2016-06-15 沃维公司 甜菊糖苷的重组生产
DK2620428T3 (da) 2010-09-24 2019-07-01 Wave Life Sciences Ltd Asymmetrisk hjælpegruppe
CN107365339A (zh) 2011-07-19 2017-11-21 波涛生命科学有限公司 合成官能化核酸的方法
EP2742142B2 (en) 2011-08-08 2020-03-25 Evolva SA Recombinant production of steviol glycosides
WO2013157953A1 (en) 2012-04-20 2013-10-24 Merus B.V. Methods and means for the production of ig-like molecules
US10829772B2 (en) 2012-06-22 2020-11-10 Monsanto Technology Llc Unique modular vector design
SG11201500243WA (en) 2012-07-13 2015-04-29 Shin Nippon Biomedical Lab Ltd Chiral nucleic acid adjuvant
WO2014010250A1 (en) 2012-07-13 2014-01-16 Chiralgen, Ltd. Asymmetric auxiliary group
DK2872147T3 (da) 2012-07-13 2023-02-20 Wave Life Sciences Ltd Fremgangsmåde til fremstilling af kirale oligonukleotider
US20150293076A1 (en) * 2012-10-22 2015-10-15 Bolt Threads, Inc. Cellular Reprogramming for Product Optimization
CA2899276C (en) 2013-02-06 2022-11-01 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
US10017804B2 (en) 2013-02-11 2018-07-10 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
CA2924343C (en) 2013-09-17 2025-09-16 Bolt Threads, Inc. PROCESSES AND COMPOSITIONS FOR SYNTHESIZING IMPROVED SILK FIBERS
US9580758B2 (en) 2013-11-12 2017-02-28 Luc Montagnier System and method for the detection and treatment of infection by a microbial agent associated with HIV infection
EP3095459A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having antitumor effect and antitumor agent
WO2015108046A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤
EP3095461A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
EP3094728B1 (en) 2014-01-16 2022-03-09 Wave Life Sciences Ltd. Chiral design
MX2017001859A (es) 2014-08-11 2017-04-11 Evolva Sa Produccion de glicosidos de esteviol en hospederos recombinantes.
JP2017528134A (ja) 2014-09-09 2017-09-28 エヴォルヴァ エスアー.Evolva Sa. 組み換え宿主におけるステビオールグリコシドの生産
BR112017016338A2 (en) 2015-01-30 2018-03-27 Evolva Sa production of steviol glycosides in recombinant hosts
CA3255837A1 (en) 2015-03-16 2025-10-30 Dsm Ip Assets B.V. Udp-glycosyltransferases
JP2018522569A (ja) 2015-08-07 2018-08-16 エヴォルヴァ エスアー.Evolva Sa. 組換え宿主内でのステビオールグリコシドの産生
MY198001A (en) 2016-04-13 2023-07-25 Evolva SA Production of steviol glycoside in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
US11447532B2 (en) 2016-09-14 2022-09-20 Bolt Threads, Inc. Long uniform recombinant protein fibers
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2024137877A1 (en) * 2022-12-21 2024-06-27 Clara Foods Co. Systems and methods for high yielding recombinant microorganisms and uses thereof

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341302C (en) 1983-02-22 2001-10-09 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
AU3556789A (en) 1988-04-22 1989-11-24 Massachusetts Institute Of Technology Process for forming and using microdroplets
ES2109238T3 (es) 1989-07-07 1998-01-16 Unilever Nv Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
US5316922A (en) * 1992-04-15 1994-05-31 The United States Of America As Represented By Department Of Health And Human Services Method for indentifying and expressing proteins that recognize and adhere to specific probes
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
WO1995011986A1 (en) 1993-10-27 1995-05-04 The Trustees Of Columbia University In The City Of New York METHOD FOR GENERATING A SUBTRACTED cDNA LIBRARY AND USES OF THE GENERATED LIBRARY
US6268140B1 (en) * 1994-02-03 2001-07-31 Neugenesis Combinatorial metabolic libraries
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) * 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5824485A (en) * 1995-04-24 1998-10-20 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6004788A (en) * 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US6030779A (en) * 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) * 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US20010041333A1 (en) * 1997-06-16 2001-11-15 Short Jay M. High throughput screening for a bioactivity or biomolecule
US5939250A (en) * 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
WO2000053744A2 (en) 1999-03-09 2000-09-14 Diversa Corporation End selection in directed evolution
GB9603803D0 (en) * 1996-02-22 1996-04-24 Zeneca Ltd Production of proteins, plasmids coding therefor and organisms containing such plasmids
US6025155A (en) 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
EP0922109A1 (en) 1996-05-21 1999-06-16 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
US5763239A (en) * 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6294325B1 (en) * 1996-07-05 2001-09-25 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of thermostable multi genes and proteins and uses thereof
WO1998001573A1 (en) 1996-07-09 1998-01-15 The Government Of The United States Of America Represented By The Secretary, Department Of Health And Human Services Transformation-associated recombination cloning
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP1717322B1 (en) 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution of whole cells and organisms by recursive sequence recombination
DE69839501D1 (de) 1997-02-27 2008-06-26 Cellomics Inc System für Screening biologischer Zellen
CA2284066A1 (en) 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
EP1015612B1 (en) 1997-05-27 2005-01-26 Hanil Synthetic Fiber Co., Ltd. Process for preparing recombinant proteins using highly efficient expression vector from saccharomyces cerevisiae
GB9727512D0 (en) 1997-12-31 1998-02-25 Adprotech Plc Fuzzy genes and their application in molecular adjuvants
AU744699B2 (en) 1998-03-04 2002-02-28 Verenium Corporation Production and use of normalized DNA libraries
AU5217599A (en) 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
WO2000008212A1 (en) 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis
WO2000012680A1 (en) 1998-08-31 2000-03-09 Maxygen, Inc. Transformation, selection, and screening of sequence-shuffled polynucleotides for development and optimization of plant phenotypes
AU6048599A (en) 1998-09-18 2000-04-10 Cellomics, Inc. A system for cell-based screening
JP2004500019A (ja) 1999-03-26 2004-01-08 ディベルサ コーポレーション 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合
GB9908814D0 (en) * 1999-04-16 1999-06-09 Celltech Therapeutics Ltd Process
AU7646000A (en) 1999-11-05 2001-05-14 Novozymes A/S Methods to screen microorganisms or gene libraries for products secreted from a cell
EP1276861A2 (en) * 2000-03-24 2003-01-22 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
WO2002059297A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
CA2474161C (en) 2001-01-25 2012-03-27 Evolva Ltd. Concatemers of differentially expressed multiple genes
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
DK1364005T3 (da) 2001-01-25 2009-02-02 Evolva Ltd En metode til udvikling af en celle der har en önsket fænotype og udviklede celler
WO2002059330A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S Artificial chromosomes comprising concatemers of expressible nucleotide sequences
US20050164162A1 (en) * 2002-01-25 2005-07-28 Evolva Ltd., C/O Dr. Iur. Martin Eisenring Methods for multiple parameters screening and evolution of cells to produce small molecules with multiple functionalities
WO2003106639A2 (en) 2002-06-14 2003-12-24 Dyax Corporation Recombination of nucleic acid library members
US20030235823A1 (en) * 2002-06-24 2003-12-25 The University Of Alabama Nucleotide sequences that code for torsin genes, torsin proteins, and methods of using the same to treat protein-aggregation
DK1529110T3 (da) 2002-08-01 2012-01-30 Evolva Ltd Fremgangsmåde til blanding af store antal af heterologe gener

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7212982B1 (ja) 2022-10-05 2023-01-26 株式会社Logomix 細胞ライブラリおよびその製造方法
WO2024075756A1 (ja) * 2022-10-05 2024-04-11 株式会社Logomix 細胞ライブラリおよびその製造方法
JP2024054652A (ja) * 2022-10-05 2024-04-17 株式会社Logomix 細胞ライブラリおよびその製造方法

Also Published As

Publication number Publication date
WO2002059297A8 (en) 2004-04-15
WO2002059297A3 (en) 2003-04-10
DE60239386D1 (de) 2011-04-21
CA2474172A1 (en) 2002-08-01
DK1356037T3 (da) 2011-06-27
US7838287B2 (en) 2010-11-23
US20120094870A1 (en) 2012-04-19
AU2002227883A1 (en) 2002-08-06
CA2474172C (en) 2012-07-10
US20040241672A1 (en) 2004-12-02
EP1356037B1 (en) 2011-03-09
EP1356037A2 (en) 2003-10-29
JP2010043108A (ja) 2010-02-25
JP4965625B2 (ja) 2012-07-04
WO2002059297A2 (en) 2002-08-01
ATE501251T1 (de) 2011-03-15

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